急性脑卒中患者血清SOD及LPO变化的临床研究

测定78例急性脑卒中患者,且病程在一月以上者的血清超氧化物歧化酶(SOD)及血清脂质过氧化物酶(LPO)的活性.结果发现:血清SOD在脑出血患者发病后第1、7、14天明显降低,而脑出血患者发病后第 1、7、14天及脑梗塞患者第 1、7天血清LPO较对照组明显升高,尤以病情严重者为著.     

脑血管疾病微血管学指标改变的临床意义研究

目的 观察急性脑血管病患者微血管学相关指标改变.方法 选择急性脑血管意外患者,检测其血液流变学、血小板指标、ET及SOD等指标并作统计学处理.结果 血液流变学4项指标,脑出血组三项指标均明显高于对照组和脑梗死组;脑梗塞死组3项指标均高于对照组.脑出血和脑梗死组血小板计数和SOD均低于对照组,而ET则高于对照组.结论 急性脑血管病患者微血管学指标有明显改变,血液有流变性障碍.     

氧自由基与胎儿生长受限关系的研究

目的 探讨氧自由基(OFR)与胎儿生长受限(FGR)发生的相关性。方法 抽取70例胎儿生长受限的孕妇(FGR组)及70例正常孕妇(对照组)静脉血，用联苯三酚自氧化法检测红细胞超氧化物歧化酶(SOD)；DTNB直接法测定全血谷胱甘肽过氧化物酶(GSH-Px)活力；采用改良的硫代巴比妥酸(TBA)法测定血清脂质过氧化物(LPO)水平。结果 FGR组SOD及GSH-Px活力较对照组明显下降，而LPO水平明显增高。结论 氧自由基与胎儿宫内生长受限的发生有关，检测孕妇血SOD，GSH-Px及LPO可作为胎儿生长受限诊断依据之一。     

急性脑血管病血清心肌酶谱的变化及其意义

目的　探讨急性脑血管病血清心肌酶谱的变化特点。方法　应用日本岛津CL -72 0 0全自动生化分析仪测定2 69例急性脑血管病患者血清天冬氨酸氨基转移酶 (AST)、乳酸脱氢酶 (LDH )、磷酸肌酸激酶 (CK )、α -羟丁酸脱氢酶 (α-HBDH)。结果　脑梗塞组CK、α -HBDH值显著高于对照组 (P均 <0 .0 1) ,脑出血组AST、LDH值高于对照组 (P <0 .0 5或 <0 .0 1) ,脑出血组CK、α -HBDH值高于对照组 (P均 <0 .0 1) ,脑出血组LDH、α -HBDH值显著高于脑梗塞组(P均 <0 .0 1)。结论　脑梗塞和脑出血血清心肌酶谱变化有差异 ,心肌变化与急性脑血管病的病变性质和程度、心脏受损情况及预后有关 ,心肌酶谱可作为判断病情轻重及预后的早期参考指标之一     

急性缺血性中风血中自由基含量的变化

为探讨自由基与缺血性中风之关系,我们对46例急性缺血性中风患者血中过氧化脂质(LPO)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH—P_x)进行了测定。结果表明,急性缺血性中风病人血浆LPO均值均高于对照组,红细胞SOD及全血GSH—P_x均值均低于对照组。表明急性缺血性中风病人体内曾发生明显的脂质过氧化反应,说明自由基曾参与急性缺血性中风致使的继发性脑损伤。     

苦碟子注射液对26例肺心病患者抗脂质过氧化损伤的影响

目的　探讨苦碟子注射液对肺心病患者抗脂质过氧化损伤的影响。方法　肺心病患者在常规治疗基础上加用苦碟子注射液20ml/d静点治疗,连用2周,监测治疗前后血清脂质过氧化物(LPO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的变化及临床疗效并与对照组对照分析。结果　两组患者治疗前LPO、SOD高于正常对照组,GSH-Px明显低于正常对照组。苦碟子注射液治疗后,GSH-Px增加,LPO、SOD降低,均恢复正常。常规治疗组GSH-Px、LPO及SOD治疗前后无显著性差异(P>0. 05),苦碟子治疗组疗效优于常规治疗组,总有效率差异有显著性(χ2 =5 03,P<0. 05)。结论　苦碟子注射液有减轻肺心病患者抗脂质过氧化损伤的作用,是治疗肺心病的一种有效的辅助治疗药物。     

脂质与脑血管病关系的探讨

为了揭示脂质代谢在动脉血栓性脑梗塞和脑出血之间的并同;及其与不同部位梗塞的关系,评价脂质在脑血管病中的作用。采用酶法对544例不同民族的急性脑血管病(梗塞463例,出血81例)做了血脂分析,并与对照组407例进行比较。结果显示:脑梗塞组和脑出血组的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL—C)及总胆固醇与高密度脂蛋白胆固醇的比值(TC/HDL—C)均非常显著高于对照组(P<0.001),脑梗塞组的HDL—C非常显著低于对照组(P<0.001);梗塞组和出血组之间、及梗塞组中深穿支闭塞亚组与皮层支闭塞亚组之间各血脂成分对比,无显著差异(P>0.05);单纯脑血管病组及合并高血压、糖尿病、冠心病组分别与正常组对比、单纯脑血管病组与脑血管病各合并症组对比,差异不一,提示血脂异常是脑血管病不可忽视的因素。脂质与脑血管病的关系,复杂而有待于深入研究。     

脑血栓与脑出血血脂及脂蛋白改变的研究

为了揭示脂质代谢在动脉血栓性脑梗塞和脑出血之间的并同;及其与不同部位梗塞的关系,评价脂质在脑血管病中的作用。采用酶法对544例不同民族的急性脑血管病(梗塞463例,出血81例)做了血脂分析,并与对照组407例进行比较。结果显示:脑梗塞组和脑出血组的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL—C)及总胆固醇与高密度脂蛋白胆固醇的比值(TC/HDL—C)均非常显著高于对照组(P<0.001),脑梗塞组的HDL—C非常显著低于对照组(P0.05);单纯脑血管病组及合并高血压、糖尿病、冠心病组分别与正常组对比、单纯脑血管病组与脑血管病各合并症组对比,差异不一,提示血脂异常是脑血管病不可忽视的因素。脂质与脑血管病的关系,复杂而有待于深入研究。     

急性脑梗塞病人血超氧岐化酶及脂质过氧化物测定

测定25例急性脑梗塞患者和66例正常老年人的血清超氧岐化酶(SOD),脂质过氧化物(LPO),并对其中9例患者作治疗前后对照观察。结果显示:急性脑梗塞患者SOD水干较正常老年人明显下降(P<0.05),而LPO则明显升高(P<0.01)。治疗后SOD水平继续下降(P<0.05),而LPO水平已有下降(P<0.05)。表明急性脑梗塞患者体内自由基产生增加,使SOD等抗氧化剂大量消耗,其含量下降。而脂质过氧化作用加强,LOP水平升高,治疗后下降。     

脑血管病患者血清高密度脂蛋白亚组分的测定

255例不同时期脑血管病惠者,急性脑梗塞惠者101例,男55例,女46例,平均年龄59.67±10.42岁;高血压脑出血43例,男31例,女12例,平均年龄60.44±7.93岁;恢复期脑梗塞患者28例,男17例,女11例,平均年龄59.26±6.49岁;脑动脉硬化惠者83例,男46例,女37例,平均年龄56.44±5.37岁。对其血清中的高密度脂蛋白胆固醇(HDL-C)及亚组分进行了测定。结果发现,脑动脉硬化组、急性脑梗塞组、高血压脑出血组、恢复期脑梗塞组患者血清中的HDL-C 及HDL-2C 均较正常对照组明显降低(P<0.001),HDL-3C则变化不大。而不同时期脑血管病患者的低密度脂蛋白(LDL-C)较正常对照组明显升高。作者认为HDL-C 尤其是HDL-2C 的下降或许是发生脑血管病的危险因素。     

脂质过氧化与冠状动脉粥样硬化性心脏病

对31名心绞痛或陈旧性心肌梗塞的男性冠心病患者及42名健康男性进行血液检测,冠心病患者的血清过氧化脂质(LPO) 含量较健康者显著增高,而血铜,锌,硒含量及谷胱甘肽过氧化物酶(GSH—Px) 和超氧化物岐化酶(SOD) 活力均较健康者显著降低。多因素分析结果,冠心病患者血LPO与GSH-Px,SOD及硒均呈负相关;血硒含量与GSH-Px活力及血铜含量与SOD活力均呈正相关。脂质过氧化增强在冠心病的发生发展过程中可能起着重要的促进作用。     

Preventive effect of re du qing on hepatocytes and mitochondria damaged by lipid peroxidation in experimental rabbits with endotoxin-induced disseminated intravascular coagulation

In this study, the general Shwartzman reaction of rabbits induced by Escherichia Coli endotoxin was made as DIC models. The experiments showed that the levels of lipid peroxide (LPO) in hepatic tissue and mitochondria in the model group were increased significantly compared with the control group (P less than 0.01), while superoxide dismutase (SOD) activity in hepatic tissue and glutathione peroxidase (GSH-Px) activity in hepatic tissue and mitochondria were decreased significantly (P less than 0.01). The levels of LPO in hepatic tissue and mitochondria in Re Du Qing (RDQ) group and vitamin E (VE) group were decreased significantly (P less than 0.01 and P less than 0.05 respectively) compared with the model group. The levels of LPO in the RDQ group did not differ from the control group (P greater than 0.05), but the levels of LPO in the VE group were still higher than those in the control group significantly (P less than 0.05). The SOD activity in hepatic tissue and GSH-Px activity in hepatic tissue and mitochondria in both RDQ group and VE group were also significantly higher than those in the model group (P less than 0.01). These data suggest that the levels of oxygen free radicals were increased in hepatocytes and mitochondria. This is related to the decreased activities of SOD and GSH-Px in the course of pathogenesis of endotoxin-induced DIC. This study indicates that lipid peroxidation might be one of the important mechanisms resulting in hepatocellular and mitochondria from oxidative damage.     

肺良恶性疾病患者血LPO、SOD、GSH-Px测定及临床意义

测定了10例健康人、20例肺良性疾病及15例肺癌患者血LPO含量、GSH-Px活性、SOD活性及SOD/LP0比值,结果①肺癌组SOD活性,SOD/LPO比值均显著低于正常组及良性组(P<0.01;<0.05;<0.01;<0.01);②正常组及良性组SOD活性及SOD/LPO比值差别无显著性(分别P>0.05);③LPO含量及GSH-Px活性三组之间差别无显著性(分别P>0.05);④若将正常组SOD及SOD/LPO比值的均数分别减去一个标准差(x-s)作为肺癌的诊断试验阳性标准,其灵敏度分别为73.3％和73.3％,特异度为80％和70％;诊断符合率为77.1％和71.4％。上述结果提示肺癌的发生发展可能与氧自由基损伤有关。通过SOD活性及SOD/LPO比值的测定可作为鉴别肺部良恶性疾病的辅助诊断手段之一,对肺癌的辅助诊断也有一定价值。     

Effects of compound Salvia Injection on lipid peroxidation antioxidant enzymes activity in patients with chronic cor pulmonale

The effects of Compound Salvia Injection (CSI) on plasma lipid peroxidation (LPO), RBC superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activity in patients with chronic cor pulmonale were studied. Thirty patients were randomly divided into two groups: the group treated with routine therapy (routine group) and the group treated with routine therapy plus CSI (CSI group). The 4 parameters mentioned above have been measured in patients of the two groups and in 22 healthy subjects for control. The results showed: (1) Before treatment, both the treatment groups had significantly higher LPO and SOD and markedly lower GSH-Px and CAT level than those of the healthy control (P<0.001). (2) After treatment the 4 parameters have been normalized in CSI group. (3) In the routine group, the parameters were still abnormal, that is, the increased levels of GSH-Px and CAT and the decreased LPO were singificantly greater in CSI group than that in routine group. These results indicated that CSI could obviously lower the enhanced lipid peroxidation and adjust the imbalance of antioxidant enzymes.     

EffectsofCompoundSalviaInjectianonLipidPeroxidationAntioxidantEnzymesActivityinPatientswithChronicCorPulmonale

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Clinical Study on Zhuyu Tongfu (逐瘀通腑) Serial Recipe Combined with Acupuncture and Massotherapy in Treating Hypertensive Cerebral Hemorrhage

Hypertensive cerebral hemorrhage is acommon ail ment that seriouslythreatens hu-man being's life and health,the mortalityand disability is very high.Integrated clini-cal and experi mental study proved that thebasic pathogenesis of this ail ment is intrace-rebral blood stasis,so the principle of acti-vating blood circulation and removing stasisto dispel blood stasis was effective to a cer-tain extent.Since3years before,we haveused Zhuyu Tongfu(逐瘀通腑,ZYTF)Seri-al Recipe combined with acupu…     

自由基损伤对乳腺癌患者抗氧化能力影响

对１４例乳腺癌患者全血谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）、红细胞超氧化物歧化酶（ＳＯＤ）的活性以及血浆脂质过氧化物（ＬＰＯ）含量进行测定，结果表明乳腺癌患者ＧＳＨ－Ｐｘ及ＳＯＤ活性均比正常人明显降低，ＬＰＯ含量明显升高，ＧＳＨ－ＰＸ／ＬＰＯ、ＳＯＤ／ＬＰＯ比值也明显降低，由此可见乳腺癌患者抗氧化能力降低，清除自由基功能受损，机体受自由基过氧化损伤的威胁。     

Clinical study on Zhuyu Tongfu Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage

Objective: To observe the clinical efficacy and mechanism of Zhuyu Tongfu (ZYTF) Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage (HCH).Methods: One hundred and eighteen patients with hypertensive cerebral hemorrhage, on the basis of conventional Western medicine treatment, were randomly divided into ZYTF combined with acupuncture and massotherapy group (treated group) and simple Western medicine group (control group); the clinical efficacy, neurofunction deficit scoring (NDS) alterations and hematoma absorption rate of both groups were observed, and also the plasma Superoxide dismutase (SOD) activity, plasma lipid peroxidase (LPO) content, erythrocyte glutathion peroxidase (GSH-Px) activity, hematocrit (Ht) and the whole blood viscosity (Va) change were also observed.Results: In the treated group, the clinical efficacy, NDS improvement and hematoma absorption rate were superior to that of the control group; comparison between the two groups after treatment showed that plasma SOD activity and GSH-Px activity got more elevated and plasma LPO content, Ht and Va more lowered in the the treated group than those in the control group.Conclusion: ZYTF combined with acupuncture and massotherapy has better effect, its therapeutic mechanism was possibly correlated to the elevation of plasma SOD activity, GSH-Px activity and lowering of plasma LPO content, Ht and Va. Supported by the Programs Foundation of Cerebrovascular Disease Treated by Integrative Traditional Chinese and Western Medicine of the Key Subject of TCM of Zhejiang Province (No. 2001-18)     

魔芋葡甘聚糖对糖尿病大鼠脂质过氧化物和抗氧自由基酶类活性的影响

目的 探讨魔芋葡甘聚糖治疗病的作用机制。方法 观察魔芋甘聚糖对四氧嘧啶实验性糖尿病大鼠血溏水平以及脂质过氧化物（LPO）含量和抗氧自由基酶类活性的影响。对正常对照组、糖尿病对照组和喂饲魔芋葡甘聚糖组大鼠体内的上述指标进行检测。结果 魔芋葡甘聚糖组大鼠血糖水平明显低于对照组（P＜0．01）;血浆、胰腺和肌肉LPO含量显著低于糖尿病对照组（P＜0．05）;超氧化物歧化酶（SOD）活性在肝、胰腺和肌肉内明显高于糖尿病对照组（P＜0．05）;红细胞、肝和肌肉内的谷胱甘肽过氧化物酶（GSH－Px）活性明显高于糖尿病对照组（P＜0．05）;红细胞、肝和肌肉的谷胱甘肽过氧化物酶（GSH－Px）活性明显高于糖尿病对照组（P＜0．05）。结论 魔芋葡甘聚糖具有降低实验性糖尿病大鼠血糖的作用,其与提高抗氧自由基酶类活性及抗过氧化损伤有密切关系。     

川芎嗪治疗胎儿宫内生长迟缓中氧自由基的变化

测定212例妇女氧自由基（OFR）的变化,其中正常非孕妇18例、孕妇136例;胎儿宫内生长迟缓（IUGR）58例,分为川芎嗪冶疗组（川组）47例,氨基酸治疗组（氨组）11例。测定母血红细胞超氧化物歧化酶（SOD）、全血谷胱甘肽过氧化物酶（GSH-Px）及血清脂质过氧化物（LPO）。结果显示生理妊娠时随孕龄增加,SOD、GSH-Px活力及LPO水平均同步上升;IUGR者LPO异常增高而SOD及GSH-Px活力显著下降,治疗后上述变化可接近或恢复正常,治疗总有效率川组达95.74％,显著高于氨组（8l.82％）,P<0.05,差异有显著性意义。研究表明妊娠正常与否与体内氧化及抗氧化系统的平衡有关,IUGR者此平衡紊乱;氧自由基异常增多及其清除剂减少,即过氧化、氧化与抗氧化系统失衡。川芎嗪是治疗IUGR的有效药物之一,可抑制OFR的生成,增强SOD、GSH-Px活力,促进胎儿生长发育。