尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

目的 探讨辅助性T淋巴细胞(Th细胞)和细胞毒性T淋巴细胞(Tc细胞)的极化情况在尖锐湿疣(CA)发病机理中的作用及其与疾病复发的关系.方法 采用三色荧光抗体染色流式细胞术检测细胞内细胞因子的方法 ,检测30例CA患者和20名健康对照者外周血CD3+CD8-/IFN-γ+(Th1)、CD3+CD8-/IL4+(Th2)、CD3+CD8+/IFN-γ+(Tc1)和CD3+CD8+,IL-4+(Tc2)细胞比例.结果 与健康对照组比较,CA患者外周血中Thl细胞含量显著减少(P<0.01),Tc1细胞含量、Th1/Th2比值、Tc1/Tc2比值均减少(P<0.05);其中15例复发CA患者的Th1/Th2比值较正常对照显著减少(P<0.01),而Te1/Te2比值与健康对照组比较差异无统计学意义.结论 CA患者外周血Th1和Tc1细胞数减少,而Th2和Tc2细胞相对占优势,复发的CA患者这种失衡更趋严重.CA患者机体免疫状态可能存在Th1→Th2、Tc1→Tc2方向的漂移,这种漂移可能是人乳头瘤病毒(HPV)不易被有效清除或CA反复发生的一种免疫机制.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

系统性红斑狼疮患者CD28表达及其意义

ＣＤ２８是Ｔ细胞激活中重要的共刺激分子。为了解Ｂ７－Ｃ礤刺激途径在系统性红斑狼疮（ＳＬＥ）中的作用，我们对３０例期ＳＬＥ２外周血Ｔ细胞ＣＤ２８的表达进行了检测，并分析了其激活后凋亡情况。结果表明，ＳＬＥ组的ＣＤ２８Ｔ细胞低于正常对照组，在抗ＣＤ３单抗刺激后ＣＤ２８细胞凋亡率增加。这提示ＳＬＥ中Ｂ７－ＣＤ２８共刺激途径介导的ＡＩＣＤ可能导致ＳＬＥ中的Ｔ细胞淋巴细胞贫血症。     

SLE患者外周血T细胞亚群ICOS与CD28共表达水平与疾病的关系

目的观察系统性红斑狼疮(systemic lupus erythematosus，SLE)病人外周血CD4^ 及CD8^ T细胞表面可诱导共刺激分子(inducible co-stimulator，ICOS)及CD28共表达水平，探讨ICOS和CD28共表达水平与SLE疾病的关系。方法采用三色流式细胞术检测SLE病人(n=51)及正常人(n=30)外周血CD4^ 及CD8^ T细胞表面ICOS与CD28共表达水平，并结合SLE病人疾病活动程度、临床表现等进行分析。结果与正常人相比，SLE活动期和稳定期病人外周血CD4^ T细胞中仅表达ICOS不表达CD28(即CD28^-ICOS^ )的细胞比例明显升高，但活动期病人和稳定期病人之间并无差异；活动期病人外周血CD8^ T细胞上同时表达CD28和ICOS的细胞(即CD28^ ICOS^ 细胞)和CD28^-ICOS^ 细胞比例都明显升高；同一SLE病人在疾病活动期CD4^ 和CD8^ T细胞中CD28^ ICOS^ 的细胞比例和CD8^ T细胞中CD28-ICOS^ 细胞比例明显高于该病人经过治疗病情稳定时；初发未治疗SLE病人仅CD4^ T细胞中CD28^ ICOS^ 细胞的比例比复发病人明显升高；血清抗dsDNA抗体( )病人和血清免疫球蛋白含量(IsG、IgA和IgM中任何一种)异常升高的病人外周血CD4^ T细胞中CD28^ ICOS^ 细胞和CD8^ T细胞中CD28-ICOS^ 细胞比例分别明显高于血清抗dsDNA抗体(-)和血清免疫球蛋白含量正常的病人。结论CD28和ICOS在SLE病人外周血CD4^ 和CD8^ T细胞上共表达水平与SLE疾病的活动程度、病程及临床表现等存在一定的关联。     

系统性红斑狼疮外周血单个核细胞CD28/B7分子mRNA的表达

目的 :探讨CD2 8 B7分子在系统性红斑狼疮 (SLE)发病机制中的作用及其临床意义。方法 :应用逆转录 聚合酶链反应 (RT PCR)检测 35例活动期SLE患者和 30例正常人外周血单个核细胞 (PBMC)中CD2 8、B7 1和B7 2mRNA的表达水平。结果 :35例活动期SLE患者PBMC中CD2 8的阳性表达率 (2 2 86 % )明显低于正常人对照组 (70 0 0 % ) ,差异非常显著 (P <0 0 0 1) ;B7 2的阳性表达率 (82 86 % )明显高于正常对照组 (5 3 33% ) ,差异显著 (P <0 0 1) ;活动期SLE组CD2 8的平均表达水平 (0 194 5± 0 2 0 74 )明显低于正常对照组 (0 4 2 38± 0 10 5 3) ,差异显著 (P <0 0 5 ) ;B7 2的平均表达水平 (0 86 75± 0 2 5 75 )明显高于正常人对照组 (0 4 898± 0 30 72 ) ,差异非常显著 (P <0 0 1) ;35例活动期SLE患者中仅有 2例B7 1呈阳性表达。结论 :CD2 8 B7分子的异常表达可能与SLE患者淋巴细胞和抗原呈递细胞 (APC)的功能变化有关。B7 1低水平与B7 2的高水平表达表明 ,SLE患者T细胞的活化可能主要是通过CD2 8与B7 2的交联传递共刺激信号 ,介导以Th2型反应为主的免疫应答反应 ;B7 2的表达水平可能与SLE疾病的活动性有一定的相关性。CD2 8mRNA的低水平表达可能与外周血CD2 8 T细胞凋亡增加或迁移到炎症部     

伴颈动脉粥样硬化脑梗患者CD4+CD28null T细胞CD158j表达和ERK磷酸化水平的关系

目的 探讨伴颈动脉粥样硬化(CA)脑梗患者CD4+CD28null T细胞上CD158j的表达百分率和ERK磷酸化(p-ERK)水平的关系及对颈动脉粥样斑块不稳定的影响.方法 采用流式细胞术检测106例伴CA脑梗、33例颈动脉正常脑梗患者和50例健康人外周血CD4+CD28null T细胞数量,以及CD4+CD28null T细胞CD158j和穿孔素(perforin)的表达,36例斑块不稳定患者CD4+T细胞p-ERK水平.ELISA法检测血清IFN-γ水平.B超检查以上人群颈动脉血管内壁粥样硬化情况.结果 脑梗患者CD4+CD28nullT细胞数量、CD4+CD28null T细胞CD158j和perforin的表达,以及血清IFN-γ水平,均明显高于健康对照组(P均<0.01);CD4+CD28null T细胞数量、CD4+CD28null T细胞CD158j和perforin的表达,以及血清IFN-γ水平,在脑梗患者中由高到低依次为颈动脉不稳定斑块组、颈动脉稳定斑块组、颈动脉内-中膜厚度(IMT)增厚组和颈动脉正常组.颈动脉不稳定斑块脑梗患者CD4+CD28null T细胞CD158j的表达与p-ERK水平呈显著性正相关(P<0.01).结论 伴CA脑梗患者CD4+CD28null T细胞数量明显增多.CD158j可能通过上调p-ERK水平,促进CD4+CD28null淋巴细胞增殖,产生细胞毒效应和分泌细胞因子,最终导致颈动脉粥样斑块不稳定.

Abstract：

Objective To investigate the relationship between CD158j expression and phosphorylated ERK (p-ERK) in CD4+ CD28null T cells in cerebral infarction (CI) patients- with carotid atherosclerosis and its effects on carotid atherosclerotic plaque stability. Methods Percentage of peripheral CD4+ CD28null and the expression of CD158j and perform on CD4+ CD28null cells was analyzed with flow cytometry in 106 CI patients with carotid atherosclerosis, 33 CI patients with normal carotid arteries and in 50 normal controls, respectively; p-ERK expression was assayed with flow cytometry in 36 CI patients with unstable plaque, and serum IFN-γ was detected with ELISA. The intima-media thickness (IMT) of bilateral carotid arteries in all subjects was confirmed by the colour Doppler ultrasonograph imagingResults Percentage of the CD4+ CD28null T cells, expression of CD158j and perform on CD4+ CD28null T cells and the serum IFN-γ levels was dramatically higher in CI patients than that in normal controls, respectively (all P <0.01), which was decreased in an order of CI patients with patients with unstable plaque, stable plaque, carotid artery IMT and with normal carotid artery. A strong positive correlation was observed between the CD158j expression and degree of p-ERK in CI patients with unstable plaque (P < 0. 01). Conclusion CD4+ CD28null T cells were significantly increased in CI patients with carotid atherosclerosis. CD158j might up-regulate p-ERK expression and induce the proliferation of the CD4+ CD28nullT cells; consequently, higher cytokine production such as IFN-γ produced by CD4+ CD28null T cells may cause the formation of unstable plaque.     

伴颈动脉粥样硬化脑梗患者CD4+CD28null T细胞CD158j表达和ERK磷酸化水平的关系

Objective To investigate the relationship between CD158j expression and phosphorylated ERK (p-ERK) in CD4+ CD28null T cells in cerebral infarction (CI) patients- with carotid atherosclerosis and its effects on carotid atherosclerotic plaque stability. Methods Percentage of peripheral CD4+ CD28null and the expression of CD158j and perform on CD4+ CD28null cells was analyzed with flow cytometry in 106 CI patients with carotid atherosclerosis, 33 CI patients with normal carotid arteries and in 50 normal controls, respectively; p-ERK expression was assayed with flow cytometry in 36 CI patients with unstable plaque, and serum IFN-γ was detected with ELISA. The intima-media thickness (IMT) of bilateral carotid arteries in all subjects was confirmed by the colour Doppler ultrasonograph imagingResults Percentage of the CD4+ CD28null T cells, expression of CD158j and perform on CD4+ CD28null T cells and the serum IFN-γ levels was dramatically higher in CI patients than that in normal controls, respectively (all P <0.01), which was decreased in an order of CI patients with patients with unstable plaque, stable plaque, carotid artery IMT and with normal carotid artery. A strong positive correlation was observed between the CD158j expression and degree of p-ERK in CI patients with unstable plaque (P < 0. 01). Conclusion CD4+ CD28null T cells were significantly increased in CI patients with carotid atherosclerosis. CD158j might up-regulate p-ERK expression and induce the proliferation of the CD4+ CD28nullT cells; consequently, higher cytokine production such as IFN-γ produced by CD4+ CD28null T cells may cause the formation of unstable plaque.     

伴颈动脉粥样硬化脑梗患者CD4+CD28null T细胞CD158j表达和ERK磷酸化水平的关系

Objective To investigate the relationship between CD158j expression and phosphorylated ERK (p-ERK) in CD4+ CD28null T cells in cerebral infarction (CI) patients- with carotid atherosclerosis and its effects on carotid atherosclerotic plaque stability. Methods Percentage of peripheral CD4+ CD28null and the expression of CD158j and perform on CD4+ CD28null cells was analyzed with flow cytometry in 106 CI patients with carotid atherosclerosis, 33 CI patients with normal carotid arteries and in 50 normal controls, respectively; p-ERK expression was assayed with flow cytometry in 36 CI patients with unstable plaque, and serum IFN-γ was detected with ELISA. The intima-media thickness (IMT) of bilateral carotid arteries in all subjects was confirmed by the colour Doppler ultrasonograph imagingResults Percentage of the CD4+ CD28null T cells, expression of CD158j and perform on CD4+ CD28null T cells and the serum IFN-γ levels was dramatically higher in CI patients than that in normal controls, respectively (all P <0.01), which was decreased in an order of CI patients with patients with unstable plaque, stable plaque, carotid artery IMT and with normal carotid artery. A strong positive correlation was observed between the CD158j expression and degree of p-ERK in CI patients with unstable plaque (P < 0. 01). Conclusion CD4+ CD28null T cells were significantly increased in CI patients with carotid atherosclerosis. CD158j might up-regulate p-ERK expression and induce the proliferation of the CD4+ CD28nullT cells; consequently, higher cytokine production such as IFN-γ produced by CD4+ CD28null T cells may cause the formation of unstable plaque.     

Upregulated BclGL expression enhances apoptosis of peripheral blood CD4 T lymphocytes in patients with systemic lupus erythematosus

Increased lymphocyte apoptosis has been suggested to contribute to the development of systemic lupus erythematosus (SLE), but the critical factors involved in the apoptotic pathways are still unknown. By long serial analysis of gene expression (LongSAGE) profiles and microarray analyses, a novel apoptosis-related gene BclG_L expression was found significantly increased in peripheral blood CD4⁺ T cells of SLE patients, which was correlated with the enhanced CD4⁺ T cells apoptosis, anti-nuclear antibody (ANA) titer and proteinuria. In vitro, BclG_L expression could be specially upregulated by SLE serum stimulation and positively correlated with induced CD4⁺ T cell apoptosis. Enforcing BclG_L overexpression by lentivirus could directly enhance CD4⁺ T cell apoptosis, but these apoptosis-inducing effects could be partially inhibited by knockdown of BclG_L expression. Collectively, these results indicate that increased BclG_L expression may contribute to the aberrant CD4⁺ T cell apoptosis which causes an inappropriate immune response and impaired homeostasis in SLE.     

系统性红斑狼疮患者CD4+T细胞上CD137的表达

目的:探讨系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者CD4 T细胞上共刺激分子CD137的表达及其作用机制。方法:应用流式细胞术检测30例系统性红斑狼疮患者和20例正常对照者外周血T细胞活化前后CD137的表达。结果:活动期SLE患者CD4 T细胞表达的CD137明显高于稳定期及正常对照组(表达百分率分别为21.56±4.08、3.01±0.09和1.24±0.12,P<0.01),稳定期SLE患者表达的CD137与正常对照组比较无统计学差异(P>0.05)。但是活动期和稳定期SLE患者的CD4 T细胞用抗CD3单抗体外刺激活化后表达的CD137均显著高于正常对照组(表达百分率分别为56.25±9.11、27.26±3.41和13.17±1.54,P<0.01)。另外,活动期SLE患者CD4 T细胞活化后表达的CD137与补体呈负相关关系(r=-0.447,P<0.05),与IgG和24小时尿蛋白定量呈正相关关系(r=0.451,P<0.05,r=0.245,P<0.05)。结论:活动期系统性红斑狼疮患者T细胞活化前后CD137的表达均显著增高,而且CD4 T细胞活化后CD137表达水平可能提示病情和肾脏受累程度。     

系统性红斑狼疮患者外周血CD4+和CD8+T细胞PD-1的表达和意义

目的 探讨程序性死亡分子1 (PD-1)在系统性红斑狼疮(SLE)患者外周血CD4+和CD8+T细胞上的表达及临床意义.方法 应用流式细胞仪检测51例SLE患者和38例健康对照者外周血T细胞亚群表面PD-1表达水平,比较SLE稳定组、活动组和健康对照组以及狼疮肾炎组和无狼疮肾炎组之间CD4+和CD8+T细胞表面PD-1表达的百分比,并分析其与临床表现及实验室检查数据的相关性.结果 SLE活动组CD4+T细胞PD-1表达水平高于健康对照组和不活动组,差异均有统计学意义(P＜0.05).SLE活动组、稳定组CD8+T细胞PD-1表达水平均高于健康对照组,差异有统计学意义(P＜0.05).狼疮肾炎患者CD4+PD-1+和CD8+PD-1+T细胞分别高于无狼疮肾炎患者(P＜0.01).SLE患者中抗dsDNA抗体、抗Sm抗体、抗核小体抗体阳性组外周血CD4+和CD8+T细胞PD-1表达水平均高于对应阴性组.SLE患者CD4+和CD8+T细胞PD-1表达百分率与SLE疾病活动度指数(SLEDAI)、尿蛋白定量呈正相关,与补体C3呈负相关.结论 SLE患者外周血CD4+和CD8+T细胞PD-1表达异常,与SLEDA1和自身抗体产生有明确的相关性.     

系统性红斑狼疮外周血单个核细胞CD40L的表达增高

目的:了解系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)的白细胞分化抗原40配体(CD40L)表达,探讨其在发病中的作用。方法:分离SLE患者和正常人PBMCs,采用流式细胞术,检测其在正常状况和应用植物凝集素(PHA)及地塞米松(Dex)后,CD40L的表达水平,并进行比较;分析SLE患者CD40L的表达水平和狼疮活动指数(SLEDAI)的相关性。结果:活动期SLE患者PBMCs的CD40L阳性细胞百分率(%)明显高于对照组,且高于静止期SLE患者;应用PHA处理24h后,3组PBMC表达CD40L均明显增加,但活动期SLE患者增加更明显;应用地塞米松后,SLE患者(活动期和静止期)PBMCs的CD40L表达明显减少,对照组无明显改变;SLE患者(活动期和静止期)CD40L的表达水平和SLEDAI均呈明显正相关。结论:CD40L在SLE患者PBMCs的表达增加,和疾病活动度有关;其受PHA和Dex调控,在SLE发病和病程中起重要作用。