性病门诊378例女性宫颈分泌物的HPV基因型分析

目的检测和分析性病门诊女性患者宫颈分泌物中人乳头瘤病毒(HPV)的基因型别。方法收集我院性病门诊就诊的女性患者378例,按患者有无高危接触和宫颈口有无尖锐湿疣症状划分为3组,即有接触有症状组(161例)、有接触无症状组(117例)和无接触无症状组(100例)。取3组患者脱落细胞,采用反向分子杂交法进行HPV基因分型检测。结果性病门诊378例女性宫颈分泌物HPV阳性率为56.61%(214/378),有接触有症状组、有接触无症状组和无接触无症状组的阳性率分别为98.75%(159/161)、40.17%(47/117)和8.00%(8/100),3组分别检出22种、14种和5种HPV基因型别,检出的主要型别是HPV6、11、16、58、56、18、33、68。有接触有症状组和有接触无症状组单一感染和复合感染的感染率差异无统计学意义(P0.05),在多重复合感染中,以二重和三重感染为主,最多发现八重感染。有接触有症状组低危型121例次(76.10%),高危型99例次(62.26%),有接触无症状组低危型35人次(74.46%),高危型26例次(55.32%),两组感染率较高,但差异无统计学意义(P0.05)。结论 HPV6、11、16、58、56、18、33、68是性病门诊女性患者宫颈感染HPV的主要型别,健康体检者中亦能查出,临床应加强对性病门诊女性就诊者宫颈HPV基因的检测,及对HPV阳性患者进行定期的跟踪随访,尤其是高危型和复合感染患者。     

海口市某性病门诊男性就诊者梅毒感染状况及影响因素分析

目的 分析性病门诊男性就诊者梅毒的感染状况和影响因素,为梅毒及性病防控提供依据。方法 选取2016年—2020年海南省第五人民医院性病门诊诊治的男性就诊者作为研究对象。对其开展艾滋病知识知晓、高危性行为情况调查,以及梅毒、人类免疫缺陷病毒(HIV)、丙型肝炎病毒(HCV)抗体检测,分析其影响因素。结果 2016年—2020年,共监测性病门诊男性就诊者2 028例,平均年龄(35.46±12.96)岁;在婚占56.71%;本省户籍占85.70%。其梅毒阳性率为11.39%;艾滋病知识知晓率为78.55%;近3个月与女性性工作者、临时性伴发生性行为者分别占17.31%、20.51%;与同性发生肛交性行为占2.91%,近1年曾患过性病占15.83%。多因素Logistic回归分析结果显示,性病门诊男性就诊者梅毒抗体阳性与近1年患有性病(OR=3.51,95%CI:2.48～4.98)和感染HIV(OR=6.33,95%CI:2.96～13.54)存在相关性(P<0.05)。结论 海口地区性病门诊男性就诊者的艾滋病知识知晓率偏低,存在一定的高危性行为,梅毒感染率高。应针对该人群制定针对性...     

应用FQ-PCR对性病门诊男性就诊者进行HPV-DNA分型检测

目的 应用荧光定量聚合酶链反应技术(FQ-PCR)对性传播疾病(STD)门诊男性就诊者进行人乳头瘤病毒-DNA(HPV-DNA)分型检测并对结果　进行分析.方法 采用FQ-PCR技术对232例男性STD门诊就诊者进行HPV-DNA分型检测.结果 125例可见疣体者HPV单型感染中16型、18型、6/11型分别为5例、1例、74例,多重型别复合感染中16+18型、16+6/11型、18+6/11型分别为1例、15例、4例.107例分泌物检测[配偶患尖锐湿疣(CA)、配偶患其他STD、CA治疗后复查、不洁性行为史自检、患其他性病]HPV单型感染中16型、18型、6/11型分别为6例、2例、13例,多重型别复合感染中16+18型、16+6/11型、18+6/11型分别为0例、3例、2例.结论　性病门诊男性就诊者的疣体和分泌物中的HPV-DNA分型检测结果　均以低危的6/11型为主,高危的16型和18型也可检出,要大力加强对此类人群的HPV常规检测.     

性病门诊女性就诊者及性伴口腔、生殖器HPV感染情况

目的 明确性病门诊女性就诊者口腔和生殖器HPV的检出率及其性伴HPV感染对女性就诊者口腔、生殖器HPV感染的影响.方法 招募性病门诊女性就诊者200人,最终完成入组并获得本人及其固定男性伴侣口腔、生殖器HPV标本分别各167份,采用PCR法扩增HPV MY通用引物扩增L1片段检测HPV检出率.结果 性病门诊女性口腔HP...     

性病门诊男性就诊者沙眼衣原体感染及危险因素研究

目的:了解性病门诊男性就诊者中沙眼衣原体感染的严重程度及其相关影响因素.方法:采用随机抽样法对佛山市性病门诊男性就诊者进行沙眼衣原体抗原的检查,采用面对面调查方式,利用国家艾滋病哨点监测性病门诊男性就诊者相关问卷对其行为学进行调查.使用免疫层析法试剂盒定性检测沙眼衣原体抗原.单因素分析采用2 检验,多因素分析采用二分类logistic回归模型分析.结果:调查408名男性就诊者,其中沙眼衣原体感染82人,感染率为20.1%.对沙眼衣原体感染的危险因素进行单因素分析,经多因素非条件logistic回归分析筛选出年龄(OR=3.26)、性伴数(OR=7.24)、同性性行为史(OR=18.49)为沙眼衣原体感染的主要危险因素.结论:性病门诊男性就诊者沙眼衣原体感染率偏高,应开展综合防治手段,加强门诊医疗工作和防治工作的优化结合,控制性病的传播.     

天津市性病门诊男性就诊者性病感染状况分析

目的:了解天津市性病门诊男性就诊者性病感染流行趋势和高危行为发生情况,为制定防治策略提供依据。方法:依照《全国AIDS哨点监测方案》,对性病门诊就诊者进行问卷调查,同时采集静脉血进行HIV、梅毒血清学检测。结果:2010至2014年共调查性病门诊男性就诊者4001人,最近3个月与暗娼、临时性伴、同性发生性行为比例分别为48.0%(1921/4001)、37.6%(1505/4001)、10.5%(422/4001),而既与女性发生过性行为又与男性发生过性行为者占7.4%(297/4001)。最近1年内性病患病率为10.32%(413/4001)。HIV、梅毒抗体阳性率分别为1.55%(62/4001)、19.90%(796/4001)。结论:天津市性病门诊男性就诊者性病感染率较高,存在快速传播的危险因素,应加强监测并对性病门诊就诊者开展健康教育和行为干预。     

2008～2012年惠州市艾滋病监测性病门诊哨点男性就诊者性病感染情况分析

目的:了解惠州市性病门诊男性就诊者艾滋病、梅毒及其他性病流行现状及趋势,为开展干预工作以及效果评价提供依据。方法:按照《全国艾滋病哨点监测方案》要求,收集惠州市艾滋病监测性病门诊哨点男性就诊者相关信息,数据用Excel 2003进行统计分析。结果:2008～2012年惠州市皮肤病防治研究所性病门诊每年调查400人,共2 000人,发现HIV抗体阳性21例(1.05%),梅毒64例(3.20%),HIV、梅毒阳性率呈逐年上升趋势,淋病等其他性病也有增加。结论:惠州市性病门诊男性就诊者HIV和梅毒感染率呈上升趋势,尤其是男男同性恋就诊者HIV的检出率比较高,应加强性病门诊就诊者的HIV检测及监测。     

性病门诊人乳头瘤病毒感染的流行病学调查

目的：了解性病门诊就诊者中人乳头瘤病毒（human papillomavirus,HPV）及其它性传播疾病（sexuallytransmitted disease,STD）病原体混合感染的流行情况。方法：对90例临床样本采用聚合酶链反应（polymerase chain reac-tion,PCR）技术检测尿道或宫颈拭子HPV DNA,同时检测其它STD病原体。结果：在90例就诊者中,HPV DNA阳性13例（14.4%）,女性、混合感染数多者及教育程度低者HPV感染率高（P〈0.05）,HPV感染者至少同时伴有1种其它STD病原体感染。结论：HPV在性病门诊就诊者中存在一定的感染率,HPV感染与性别、混合感染数、教育程度等相关,HPV与其它STD病原体的混合感染多见。     

妇科门诊妇女人乳头瘤病毒感染情况分析

目的:调查我院妇科门诊就诊患者人乳头瘤病毒(HPV)的感染状况、基因分型,为预防HPV感染和宫颈癌防治提供理论依据。方法:采用HPV核酸扩增分型检测试剂盒对我院980例门诊女性进行HPV检测,并对其亚型感染情况进行分析。结果:980例受试者中,220例(22.45%)HPV阳性,均按各基因型HPV阳性率计算,单一基因型感染率为16.12%(158/980),多重感染率为6.33%(62/980);其中单纯高危型感染率为17.53%(170/980),单纯低危型感染率为3.57%(35/980),高危型/低危型感染率为1.53%(15/980)。高危型最高为HPV-16(6.12%),其次为HPV-52(3.57%)、HPV-58(2.96%)、HPV-56(2.14%),低危型主要为HPV-6(2.65%)与HPV-11(2.04%)。对220例HPV阳性感染者进行液基细胞学检测(LCT)检查,LCT阳性率为38.18%,同期102例HPV阴性的LCT阳性率为1.96%,HPV阳性与同期HPV阴性妇女的LCT结果的阳性率有显著差别(χ~2=20.343,P=0.01)。结论:本地区妇科门诊就诊患者HPV阳性率较高,以高危型为主,且HPV阳性率与宫颈癌变发生率有相关性。     

2008-2012年惠州市艾滋病监测性病门诊哨点男性就诊者性病感染情况分析

目的：了解惠州市性病门诊男性就诊者艾滋病、梅毒及其他性病流行现状及趋势,为开展干预工作以及效果评价提供依据。方法：按照《全国艾滋病哨点监测方案》要求,收集惠州市艾滋病监测性病门诊哨点男性就诊者相关信息,数据用Excel 2003进行统计分析。结果：2008-2012年惠州市皮肤病防治研究所性病门诊每年调查400人,共2000人,发现HIV抗体阳性21例（1．05％）,梅毒64例（3．20％）,HIV、梅毒阳性率呈逐年上升趋势,淋病等其他性病也有增加。结论：惠州市性病门诊男性就诊者HIV和梅毒感染率呈上升趋势,尤其是男男同性恋就诊者HIV的检出率比较高,应加强性病门诊就诊者的HIV检测及监测。     

Screening HIV-infected women for cervical cancer in Thailand: findings from a demonstration project

OBJECTIVES: Although cervical cancer is an AIDS-defining illness, few HIV-infected women are routinely screened for cervical cancer in Thailand. We screened HIV-infected women for cervical cancer as a component of HIV care and assessed high-risk human papillomavirus (HPV) and cervical cancer prevalence. METHODS: From July 2003 through February 2004, HIV-infected women attending either an infectious disease clinic or a sexually transmitted infection (STI) clinic in Bangkok were tested for high-risk HPV types by Hybrid Capture 2 and screened for cervical cancer by Pap test; those with abnormal cervical cytology were referred for diagnosis and treatment. RESULTS: Two hundred ten HIV-infected women at an infectious disease clinic (n = 150) and an STI clinic (n = 60) received cervical cancer screening. The high-risk HPV prevalence was 38.6% and the prevalence of abnormal cervical cytology was 20.4%. Abnormal cervical cytology and high-risk HPV detection were associated (P < 0.001). We received pathology reports for 23 (53.5%) of 43 women, including all those with a Pap test showing high-grade squamous intraepithelial lesions; the cervical cancer prevalence was 1.9% (4 of 210; 95% confidence interval, 0.5-4.8%). CONCLUSION: The estimated prevalence of high-risk HPV and cervical cancer among HIV-infected women in Thailand was high. This emphasizes the need to integrate cervical cancer screening into HIV care.     

Gender differences in sexual biomarkers and behaviors associated with human papillomavirus-16, -18, and -33 seroprevalence

BACKGROUND: The elevated risk for incident head and neck cancer among human papillomavirus (HPV)-16-seropositive individuals has substantiated a role for HPV in the etiology of head and neck cancers. The relationship between HPV seroreactivity and prevalent oral HPV infection in men and women without cancer has yet to be investigated. GOAL: The goal of this study was to evaluate a possible association between oral HPV infection and HPV seroreactivity after adjustment for gender, sexual behaviors, and sexually transmitted disease. STUDY DESIGN: A cross-sectional study of factors associated with HPV-16, -18, and -33 seroreactivity was performed in a population of 586 men and women with and without HIV infection. Antibodies in sera were measured by use of a virus-like protein (VLP)-based enzyme-linked immunosorbent assay. Exfoliated cells from the tonsillar and oral mucosa were analyzed for the presence of 38 mucosal HPV types by polymerase chain reaction. RESULTS: Women had significantly greater seroreactivity for all HPV types investigated when compared with men (odds ratio, 4.3; 95% confidence interval, 3.0-6.0). Seroprevalence was greatest in men and women aged 35 to 45 years. Tonsillar HPV infection, oral sex with men, and HIV infection were independently associated with HPV seroreactivity in men after adjustment for age and number of sexual partners. In women, HSV-2 seropositivity and a history of sexually transmitted diseases were similarly important. Oral and tonsillar HPV infection were not associated with HPV seroreactivity in women. CONCLUSION: HPV seropositivity is associated with sexually transmitted diseases among women and possibly mucosal HPV exposures in men. Tonsillar HPV infection could impact seroprevalence, particularly in men.     

Distribution of human papillomavirus types in cervicovaginal washings from women evaluated in a sexually transmitted diseases clinic

BACKGROUND: Women evaluated for sexually transmitted diseases (STDs) may be at increased risk for human papillomavirus (HPV) infection, a condition associated with cervical dysplasia. The distribution of HPV types in such a population is unknown. GOAL: The goal was to determine the prevalence of HPV infection, the distribution of HPV types, and the type distribution in relation to cervical dysplasia in women in an STD clinic. STUDY DESIGN: Cervicovaginal lavage and Papanicolaou smear specimens were obtained from 295 women. Lavage specimens were analyzed for HPV by polymerase chain reaction/reverse blot strip assay. RESULTS: Cervical cytologic findings were abnormal for 19.7% of women. HPV DNA was detected in 49.2% of women (high-risk HPV in 42.4%). HPV positivity correlated with the degree of cytologic abnormality. In women with dysplasia, HPV types 16, 66, 83, 56, 52, and 59 were commonly detected. Specimens containing abundant HPV DNA occurred most often in women with dysplasia. CONCLUSIONS: HPV infection was common in women attending an STD clinic. Numerous individual HPV types were associated with cervical dysplasia, including "low-risk" types.     

Human papillomavirus infection in men who have sex with men participating in a Dutch gay-cohort study

BACKGROUND: To develop strategies for prevention and early treatment of human papillomavirus (HPV) anal and penile cancer, a better understanding of related sexual behavior risk factors is needed. GOAL: The goal of this study was to establish the prevalence of anal and coronal sulcus HPV in a group of men who have sex with men participating in a Dutch gay-cohort study, to identify risk factors associated with HPV infection in this group, and to investigate the presence of identical HPV types in couples with stable relationships. STUDY DESIGN: A cross-sectional study of 241 HIV-negative and 17 HIV-positive men who have sex with men visiting the sexually transmitted disease clinic of the Erasmus MC for a regular and scheduled examination. Participants underwent a routine venereological examination including HIV serologic analysis, and swabs were taken from the coronal sulcus and anus for HPV DNA testing. All subjects were asked to complete a questionnaire on sexual risk behavior. RESULTS: HPV DNA was detected at the coronal sulcus in 23.5% of the HIV-positive men and in 15.8% of the HIV-negative men (P=0.492). In anal specimens, HPV DNA was detected in 64.7% of the HIV-positive men and 32.8% of the HIV-negative men (P=0.015). High-risk HPV types (P=0.007) and 2 or more different HPV genotypes (P=0.006) were seen more often in anal specimens of HIV-positive persons than in specimens of HIV-negative persons. A factor possibly associated with the presence of anal HPV infection was a concomitant anal infection with Chlamydia trachomatis, gonococci, or herpes simplex virus (P=0.059). In only 16.7% of HPV-positive steady couples, both companions showed the presence of one or more identical HPV genotypes. CONCLUSION: In this study, anal HPV DNA was detected more often than HPV DNA at the coronal sulcus. HIV positivity was associated with a higher prevalence of high-risk, but not with low-risk HPV types, at the anus. No association was found between HIV positivity and presence of high-risk HPV at the coronal sulcus. No sexual behavioral determinants for the presence of HPV could be identified. Concomitant anal infection with C trachomatis, gonococci, or herpes simplex virus may be associated with HPV infection. In the majority of steady couples, partners were infected with different HPV types.     

Genital human papillomavirus infection among patients attending an STD clinic.

OBJECTIVES--To determine the prevalence of human papillomavirus (HPV) infection in patients, male and female, attending a clinic for sexually transmitted diseases (STDs). DESIGN--A randomly selected group of patients representative of the population studied and first-time visitors to the STD clinic, were asked to participate in the study. Samples from the skin and mucous membranes of the lower genital region were taken for cytological analysis by the polymerase chain reaction (PCR) method for HPV DNA. The patients then underwent colposcopy or peniscopy after acetic acid application. SETTING--Department of Dermatology and Venereology, University Hospital, Uppsala, Sweden. SUBJECTS--A total of 131 patients, 66 women and 65 men, attending the clinic for various reasons. RESULTS--At colposcopy/peniscopy, 18 patients (10 men and 8 women) had lesions typical of, and 24 (12 men and 12 women) suspicious of HPV infection. With the PCR technique HPV DNA was detected in 72% of the patients with typical lesions and in 54% of those with suspicious lesions. CONCLUSION--Nearly one-third or 30.5% of these randomly selected patients in a Swedish STD clinic were infected by HPV. The diagnosis was made by clinical inspection and/or by HPV DNA analysis with PCR.     

Sentinel surveillance of human immunodeficiency virus infection in sexually transmitted disease clinics in the United States.

From April 1988 through December 1989, sera obtained for syphilis testing from consecutive patients attending 98 sexually transmitted disease (STD) clinics in 37 metropolitan areas were tested for antibodies to human immunodeficiency virus (HIV) in an unlinked (blinded) survey. HIV seroprevalence in STD clinics ranged from 0 to 38.5% (median, 2.3%), with the highest rates found in the Mid-Atlantic states, Florida, and Puerto Rico. The highest median rates were found in men who have sex with men (36.1%) and heterosexual intravenous (IV) drug users (4.1%). For heterosexual persons who do not report IV drug use, median rates were highest in the 35- to 39-year-old age group for men (6.4%) and the 30- to 34-year-old age group for women (0.9%). Among persons who do not report risk behaviors for HIV infection, men had substantially higher median rates of HIV infection than women (P less than 0.001, Wilcoxon Signed Rank test), and rates were positively correlated with HIV infection rates in IV drug users in the same clinic (Pearson correlation coefficient [r] = 0.8; P less than 0.001). Among heterosexual STD clinic patients who do not report IV drug use, the median HIV infection rate for blacks (1.8%) was at least 2 times higher than the median infection rates for hispanics (0.9%) and whites (0.7%). The results of this study show that HIV infection in STD clinic patients varies by geographic area, sex, race and ethnic group, and risk behavior.(ABSTRACT TRUNCATED AT 250 WORDS)     

The association between sexually transmitted pathogens and cervical intra-epithelial neoplasia in a developing community.

OBJECTIVE--To determine the association of sexually transmitted pathogens in women with cervical intra-epithelial neoplasia (CIN). SETTING--An urban tertiary referral hospital serving a large indigent developing community. PARTICIPANTS--48 women attending a colposcopy clinic and 49 women attending a family planning clinic. METHODS--Vaginal, endocervical, rectal swab specimens and sera were collected for the detection of sexually transmitted pathogens. Cervical cytology was performed on all patients. Women attending the colposcopy clinic had confirmation of abnormal cervical cytology by colposcopic directed biopsy. RESULTS--The mean age of women with CIN (33 years) was significantly greater than that of the women without CIN (28 years) and that of the family planning group (26 years). There was a high prevalence of sexually transmitted pathogens in all women. A significantly higher prevalence of bacterial vaginosis was found in women with CIN compared to those without (50% vs 20%; p = 0.034). The human papilloma virus (HPV) was detected in 46% of women with CIN and 65% of those without CIN. Chlamydia trachomatis (21%) and Trichomonas vaginalis (39%) were detected frequently in women with CIN. C. trachomatis (14%-21%) was detected more frequently than Neisseria gonorrhoeae (3-5%) in all asymptomatic women studied. CONCLUSION--This study demonstrates a high prevalence of sexually transmitted pathogens in women with and without CIN as well as family planning clinic attenders. Bacterial vaginosis was a significant finding in women with CIN. C. trachomatis was detected in a high proportion of all women studied and found more commonly than N. gonorrhoeae. We therefore recommend that all women attending gynaecological services in a developing community be investigated and treated for sexually transmitted diseases.     

Risk factors for genital HPV DNA in men resemble those found in women: a study of male attendees at a Danish STD clinic

OBJECTIVES: Genital infection with certain types of human papillomavirus (HPV) is the most important risk factor for cervical cancer. The male sexual partner is supposed to be the vector of the infection. However, the knowledge of risk factors for genital HPV DNA in men is limited. The objective of this paper is to study the risk factors for HPV infection in men and to compare them with those found in women, including the study of whether there are different risk profiles for oncogenic and non-oncogenic HPV types. METHODS: From a sexually transmitted diseases (STD) clinic in Denmark, 216 men were consecutively included. A personal interview was done and material for genital HPV DNA detection was obtained with swabs. HPV DNA was detected by polymerase chain reaction (PCR). Odds ratios (OR) for HPV as well as for oncogenic and non-oncogenic types separately were computed with a 95% confidence interval (CI) by means of unconditional multiple logistic regresssion. RESULTS: The most important predictors of any HPV were lifetime number of sex partners (OR = 4.3; 95% CI 1.4 to 13.1 for 25-39 v 1-9 partners), young age, and being uncircumcised. The most important risk factor for oncogenic HPV types was lifetime number of partners, whereas number of partners in the past year and ever having genital warts were risk factors for the non-oncogenic HPV types. Young age predicted risk of both oncogenic and non-oncogenic HPV types. CONCLUSIONS: Most risk factors for HPV DNA detection in men resemble those found in women. As in women, the risk factor profile for the oncogenic HPV types was different from that of the non-oncogenic HPV types.     

Effect of prior sexually transmitted disease on the isolation of Chlamydia trachomatis

In developed nations, Chlamydia trachomatis is the most common sexually transmitted pathogen. To determine whether prior disease affects the probability of subsequent chlamydial infection, we took culture specimens from 2,546 men and 1,998 women attending a sexually transmitted diseases clinic. The men had nongonococcal urethritis and the women were contacts of men who had a positive chlamydial culture or nongonococcal urethritis. Significantly lower isolation rates for those with a history of sexually transmitted diseases were found for both men (29% vs. 38%; P less than 0.0001) and women (27% vs. 36%; P less than 0.0001). In addition, both men and women with previously documented chlamydial infections had a lower isolation rate at the index visit, if the previous infection occurred less than, as opposed to more than, six months earlier (men: 20% vs. 41%; P = 0.0006; women: 14% vs. 35%; P = 0.003). These relationships were found to be independent of age. However, the effect of partial immunity due to prior infection could not be distinguished from that of prior antibiotic therapy, and if such immunity does confer protection against reinfection, that protection appears to be both partial and of relatively short duration.     

Isolation of Chlamydia trachomatis from women attending a clinic for sexually transmitted diseases.

Attempts were made to isolate Chlamydia trachomatis from the cervix of 300 women attending a clinic for sexually transmitted diseases in Leeds. The women were divided into four groups; (1) 130 were consorts of men suffering from non-specific urethritis; (2) 66 were suffering from gonorrhoea, or were consorts of men suffering from this disease; (3) 56 were suffering from other sexually transmitted diseases; (4) 48 had no evidence of STD. The overall isolation rate of Chlamydia trachomatis was 20%. Positive results were obtained in 30%. of Group 1, in 27-3%. of Group 2, in 3-6%. of Group 3, and in 2-1%. of Group 4. No pathogenic sign or symptom of Chlamydia trachomatis infection of the cervix was detected.