视网膜脱离时视网膜下液的乳酸脱氢酶同功酶的测定

用立式电泳方法分析视网膜脱离和视网膜脱离伴有高度近视患者血清和视网膜下液(SRF)的乳酸脱氢酶(DDH)同功酶,发现视网膜脱离病人血清中的LDH同功酶与正常人血清的基本相同,都有LDH_1、LDH_2、LDH_3、但SRF的LDH随视网膜脱离病程的延长,同功酶的种类及含量增多。病程在6个月以上的病人,其SRF的LDH含量高于自身血清的含量。表明SRF的LDH不仅来源于血浆,而且也来自视网膜组织。     

视网膜下液中结缔组织生长因子的含量与增生性玻璃体视网膜病变的关系

目的:探讨孔源性视网膜脱离患者视网膜下液(SRF)中结缔组织生长因子(CTGF)的含量与增生性玻璃体视网膜疾病(PVR)的关系。方法:采集38例孔源性视网膜脱离患者的视网膜下液,用ELISA试剂盒测定SRF中CTGF的含量,收集的视网膜下液按PVR的严重程度、病程长短、视网膜脱离范围及裂孔大小分组后进行统计学分析。结果:38例患者视网膜下液中均含有CTGF,伴PVR的患者SRF中CTGF的平均浓度比无PVR的患者高,随着PVR的加重SRF中CTGF的浓度增高,经比较差异均有统计学意义(P<0.01),CTGF的含量与病程的长短、视网膜脱离范围相关,与性别和视网膜裂孔的大小无关。结论:在PVR形成的过程中,视网膜下液中CTGF的含量随着PVR的严重程度而增加,这可能与其参与了细胞的迁移、增殖、膜收缩有关,它为将来PVR的治疗策略提供新的思路。     

孔源性视网膜脱离患者视网膜下液维A酸含量的分析

采用高效液相色谱法研究 5 6例孔源性视网膜脱离患者的视网膜下液 (SRF)及血清维A酸的含量 ,并观察部分患者服用维生素A对其的影响。结果示 ,患者SRF中维A酸的含量与增殖性玻璃体视网膜病变 (PVR)程度、视网膜脱离范围和术前服用维生素A的天数呈正相关 (r1=0 .85 1,P <0 .0 0 ;r2 =0 .4 5 2 ,P <0 .0 1;r3=0 .4 3 1,P <0 .0 5 ) ;血清维A酸的含量与眼部病变无关 ;SRF中维A酸在口服维生素服药第 4d时显著增高。结果表明视网膜脱离后 ,色素上皮和神经上皮之间代谢关系发生紊乱 ,造成维A酸循环利用障碍。服用维生素A有利于阻止PVR的发生和发展。     

载脂蛋白E与视网膜疾病关系的研究

目的 :通过检测中国汉族人视网膜色素变性患者的载脂蛋白E表型及孔源性视网膜脱离病人视网膜下液(SRF)及血液中载脂蛋白E(apoE)的含量 ,探讨载脂蛋白E与视网膜疾病的关系。方法 :采用等电聚焦及免疫印迹方法检测正常人和视网膜色素变性患者的aopE表型及ELISA方法测定孔源性视网膜脱离病人视网膜下液(SRF)及血液中载脂蛋白E(apoE)的含量。结果 :aopE的ε4 等位基因与视网膜色素变性的发病有关联 (RR =2 5 0 71P <0 0 1)。SRF中apoE的含量与视网膜脱离的范围及时间相关 (P <0 0 1)。结论 :aopE的ε4 基因可能是视网膜色素变性发病的又一遗传因素 ;apoE与孔源性视网膜脱离的损伤修复有关     

孔源性视网膜脱离患者视网膜下液TGF-β1定量检测的意义

目的:定量测定孔源性视网膜脱离(RRD)患者视网膜下液(SRF)中转化生长因子β1(TGF-β1)的含量,探讨TGF-β1 在RRD中的临床意义.方法:采集30例行RRD复位术患者的SRF,用TGF-β1试剂盒进行检测.结果:30例RRD患者SRF中均含有TGF-β1,其含量随着网脱范围、网膜裂孔的增大而增加.结论:TGF-β1参与RRD的病理过程,可能是影响网脱发展和转归的重要细胞因子之一.     

孔源性视网膜脱离视网膜下积液中神经元特异性烯醇酶和S100B浓度的研究

目的通过对视网膜下液(SRF)中神经元特异性烯醇酶(NSE)和S100B蛋白浓度的测定,评估孔源性视网膜脱离(RRD)时视网膜的神经损伤程度。方法 92份视网膜下液的样本取自孔源性视网膜脱离患者的外路手术中,同时抽取患者血清样本作为实验组,另外抽取19份角膜捐献病人的玻璃体和20份正常人的血清样本作为对照组,采用双夹心酶联免疫吸附法(ELISA)测定实验组和对照组中NSE和S100B的浓度。结果本实验视网膜下液中的NSE和S100B蛋白浓度与这些患者及正常人血清NSE和S100B蛋白浓度的差异有统计学意义(P0.05);在病程和网脱范围间的差异均有统计学意义(P0.05)。结论在RRD的发生发展过程中存在神经元和神经胶质细胞的损伤,这种损伤随网脱范围的增大而增大,随病程延长而增大,且在2周内增长最为迅速。     

孔源性视网膜脱离患者视网膜下液蛋白质液相色谱分析

运用快速液相色谱仪对孔源性视网膜脱离患者视网膜下液蛋白质进行检测。结果表明经凝胶作用色谱柱后视网膜下液蛋白质可分成４个不同分子量级分，即：＞７０Ｋｄ，＞１０Ｋｄ，＜１０Ｋｄ及＜１ｋｄ；视网膜下液蛋白质浓度随增殖性玻璃体视网膜病变的加重而增加。     

孔源性视网膜脱离患者视网膜下液蛋白质液相色谱分析

运用忆速液相色谱仪对孔源性视网膜脱离患者视网膜下液蛋白质进行检测。结果表明，经表明经凝胶作用色谱柱后视网膜下液蛋白质可分成４个不同分子量级分，即：〉７０Ｋｄ，〉１０Ｋｄ，〈１０Ｋｄ及〈１ｋｄ，视网膜下液蛋白质随增压殖性下班体视网膜病挛加重崦增加。     

Study of NSE and S100B concentration in subretinal nuid of rhegmatogenous retinal detachment

目的 通过对视网膜下液(SRF)中神经元特异性烯醇酶(NSE)和S100B蛋白浓度的测定,评估孔源性视网膜脱离(RRD)时视网膜的神经损伤程度.方法 92份视网膜下液的样本取自孔源性视网膜脱离患者的外路手术中,同时抽取患者血清样本作为实验组,另外抽取19份角膜捐献病人的玻璃体和20份正常人的血清样本作为对照组,采用双夹心酶联免疫吸附法(ELISA)测定实验组和对照组中NSE和S100B的浓度.结果 本实验视网膜下液中的NSE和S100B蛋白浓度与这些患者及正常人血清NSE和S100B蛋白浓度的差异有统计学意义(P＜0.05) ;在病程和网脱范围间的差异均有统计学意义(P＜0.05).结论 在RRD的发生发展过程中存在神经元和神经胶质细胞的损伤,这种损伤随网脱范围的增大而增大,随病程延长而增大,且在2周内增长最为迅速.     

孔源性视网膜脱离患者视网膜下液维A酸含量的分析

采用高效液相色谱法研究５６例孔源性视网膜脱离患者的视网膜下液（ＳＲＦ）及血清维Ａ酸的含量,并观察部分患者服用维生素Ａ对其的影响。结果示,患者ＳＲＦ中维Ａ酸的含量与增殖性玻璃体视网膜病变（ＰＶＲ０程度,视网膜脱离范围和术前服用维生素Ａ的天数呈正相关;血清维Ａ酸的含量与眼部病变无关;ＳＲＦ中维Ａ酸在口服维生素服药第４ｄ时显著增高。     

高度近视伴视网膜脱离患者的锌、铜代谢

30例高度近视伴原发性视网膜脱离患者血清锌、铜测定值分别为11.87±2.57μmol/L和10.94±3.90μmol/L。两者与对照组(15例)相比均有高度显著性(P<0.01).而男女之间锌、铜含量差异无显著性。本文探讨了微量元素锌,铜在高度近视伴有原发性视网膜脱离中的作用。     

Persistent Subretinal Fluid after Successful Scleral Buckle Surgery for Macula-off Retinal Detachment

Background  After successful scleral buckle and cryotherapy for macular-off retinal detachment (RD), some patients have poor visual acuity without any clinically detected macular change, and the unsatisfactory postoperative visual acuity is difficult to explain. The purpose of this study was to determine the characteristics of subretinal fluid (SRF) after successful scleral buckle surgery for macula-off RD.

Methods  Twenty-eight eyes from 28 patients were included in this study. The patients underwent scleral buckle surgery combined with cryopexy for macular-off RD. After surgery, all eyes underwent thorough ophthalmologic examinations including slit-lamp biomicroscopy, best-corrected visual acuity (BCVA) test, and binocular indirect ophthalmoscopy. The BCVA prior to the operation, duration of RD, the duration of postoperative retinal reattachment, BCVA when SRF was observed, period required for the SRF to become undetectable, and the BCVA at the final follow-up were included in the clinical data for this study. Optical coherence tomography (OCT) and B-ultrasonography were used to confirm SRF, and fluorescein angiography (FFA) was carried out in several patients. BCVA when SRF was observed and BCVA at the final follow-up were evaluated using a paired t test. Correlations between BCVA before the operation and duration of RD and BCVA at the final follow-up were analyzed using the Pearson correlation test. The type of SRF under OCT and BCVA at the final follow-up were compared using one-way analysis of variance (ANOVA).

Results  Postoperative retinal reattachment was achieved in 1.0–7.0 days (average, (2.7±2.1) days). After retinal reattachment, SRF was detected in all eyes by OCT 2 weeks postoperative, while B ultrasonography found no changes. The mean logMAR BCVA was 0.35±0.27 at the time of the detection of SRF. The period for SRF to become undetectable ranged from 2.0 to 11.0 months (average, (6.3±2.3) months). The time of follow-up ranged from 24.0 to 36.0 months (average, (28.9±3.4) months). The mean logMAR BCVA improved to 0.30±0.23 at the final follow-up, which was significantly different from the mean logMAR BCVA at the time of SRF detection (paired t-test, t=3.82, P <0.05). Postoperative OCT images were classified into three categories. FFA was carried out in 10 eyes and revealed no leakage or strain in the lesions. BCVA before the operation was significantly correlated with BCVA at the final follow-up (r=0.56, P <0.05). No significant correlation was observed between BCVA at the final follow-up and duration of RD (r=0.23, P=0.22). One-way analysis of variance (ANOVA) showed no significant difference between the type of SRF under OCT and BCVA at the final follow-up (F=0.21, P=0.81).

Conclusions  The presence of persistent SRF after successful scleral buckle surgery for macular-off rhegmatogenous RD may influence the BCVA or anatomic attachment. SRF was detected in all eyes by OCT at 2 weeks after the operation. Residual detachment persisted for almost a year after surgery in certain patients. Fluorescein angiography revealed no leakage or strain in the lesions.

     

The relationship between serum interleukins and T-lymphocyte subsets in patients with severe acute respiratory syndrome

Severeacuterespiratorysyndrome (SARS) ,alsoknownasinfectiousatypicalpneumonia (IAP)inChina ,iscausedbyacoronavirusmutant Thisrespiratorycontagiousdiseaseistransmittedmainlybyrespiratorydropletswithinaneardistanceandclosecontactwiththepatients Sofar ,thediseasehasbeenreportedinover 30countriesworldwide SARSpatientshavebeenadmittedintoourhospitalsinceMarch 11,2 0 0 3 Inthispaper ,wepresentthelaboratoryresultsofseruminterleukins (IL) ,T lymphocytesubsets ,whitebloodcell (WBC)countsof 35pa…     

新生豚鼠胸腺T、B淋巴细胞、吞噬细胞的定量与动态研究

采用木瓜蛋白酶处理的兔红细胞花环形成试验和兔抗豚鼠免疫球蛋白标记的免疫微球花环形成试验及乳胶颗粒吞噬试验分别测定了2～4用龄豚鼠胸腺的T淋巴细胞、B淋巴细胞和吞噬细胞。结果显出:T淋巴细胞和B淋巴细胞分别占胸腺细胞的69～78％和0.7～2.6％。胸腺吞噬细胞含量甚少。胸腺T、B淋巴细胞总数随动物周龄、胸腺重量的增加而增加。第2～3周龄平均每日增加T细胞4029万、B细胞14万;第3～4周龄平均每日增加T细胞4986万、B细胞71万。第3～4周龄比第2～3周龄动物平均每日多增加T、B淋巴细胞各57万。     

裂孔源性视网膜脱离手术中不放液的选择与疗效分析

目的：探讨视网膜裂孔冷凝、巩膜环扎、外加压、手术中不放液治疗裂孔源性视网膜脱离（ＲＲＤ）的手术适应证及疗效。方法：对７８例（８０眼）ＲＲＤ采用视网膜裂孔冷凝、巩膜环扎、外加压、术中不放液的手术方法,并对治疗结果进行分析。结果：８０眼ＲＲＤ一次手术成功率为９３．８％,视网膜下液（ＳＲＦ）在术后１～２ｄ吸收为５１．３％,３～７ｄ吸收为３５％,术后视力明显提高。结论：ＲＲＤ术中不放液为手术步骤的重要改进。因其可保持眼内液体及眼压相对稳定,手术成功率高,并发症少,适应证可适当放宽。