中药青龙衣镇痛机制研究

本文观察了青龙衣无机盐及其模拟成分对小鼠脑内钾、钙离子的影响。结果表明，Ｋ＾＋＿／Ｃａ＾＋＋比值与痛阈之间存在着相关性。青龙衣无机盐及模拟成分硫酸钾均有较强的镇痛效果；均能增加小鼠脑内钾离子含理，同时降低钙离子含量，Ｄ＾＋／Ｃａ＾＋＋比值明显增高；其镇痛作用均因注射氯化钙而被拮抗。研究表明，青龙衣无机盐是镇痛的有效成分。     

中药青龙衣镇痛机制研究：青龙衣无机盐及其模拟成分对小鼠…

用荧光分光光度计观察了青龙衣无机盐和模拟成分硫酸钾等对小鼠脑内５－ＨＴ含量变化的影响。结果表明，青龙衣无机盐和硫酸钾均能使小鼠脑内５－ＨＴ含量明显增加。     

中药青龙衣镇痛机制研究──青龙衣无机盐及其模拟成分对小鼠脑内5－羟色胺含量的影响

用荧光分光光度计观察了青龙衣无机盐和模拟成分硫酸钾等对小鼠脑内５－ＨＴ含量变化的影响。结果表明，青龙衣无机盐和硫酸钾均能使小鼠脑内５－ＨＴ含量明显增加。     

中药青龙衣镇痛机制的研究

钾盐是公认的实验用致痛物质，但青龙衣镇痛作用的活性成分是钾盐，本文通过整体动物镇痛实验及对神经干传导的影响等项药理实验表明，青龙衣无机盐，氯化钾，溴化钾及明矾均有较明显的镇痛作用，能提高小鼠基础痛阈，抑制扭体反应，并能阻断神经干及感觉神经末梢的传导，作用强度与剂量相关。     

中药青龙衣镇痛机制的研究——青龙衣无机盐与三种钾盐的镇痛作用

钾盐是公认的实验用致痛物质。但青龙衣镇痛作用的活性成分是钾盐。本文通过整体动物镇痛实验及对神经干传导的影响等项药理实验表明，青龙衣无机盐、氯化钾、溴化钾及明矾均有较明显的镇痛作用，能提高小鼠基础痛阈，抑制扭体反应，并能阻断神经干及感觉神经末梢的传导，作用强度与剂量相关     

CaCl_2对发热家兔的降温作用与CSF Na~＋／Ca~（＋＋）比值变化的关系

观察了静脉注射ＣａＣｌ２对发热家兔的降温作用与血浆和脑脊液（ＣＳＦ）中Ｎａ＋，Ｃａ＋＋含量及ＣＳＦ中Ｎａ＋／Ｃａ＋＋比值变化的关系。结果发现内毒素（ＥＴ）性发热家兔组血浆和ＣＳＦ中的Ｃａ＋＋含量明显低于正常家兔组，ＣＳＦ中的Ｎａ＋／Ｃａ＋＋比值明显高于正常家兔组。静脉注射ＣａＣＩ２引起降温效应的家兔组，除了血浆中Ｃａ＋＋含量升高外，而且ＣＳＦ中的Ｃａ＋＋含量升高到正常水平，同时使Ｎａ＋／Ｃａ＋＋比值降低到接近于正常家兔组的水平。这提示，静脉注射ＣａＣｌ２使ＥＴ性发热家兔的体温降低，可能是通过增加脑内的Ｃａ＋＋含量，降低Ｎａ＋／Ｃａ＋＋比值而实现的。     

青龙衣不同提取部位的抗肿瘤作用研究

目的 观察青龙衣不同提取部位的抗肿瘤作用。方法 制备S180和H22荷瘤小鼠模型，青龙衣不同提取部位ip给药，观察其对S180小鼠的抑瘤作用及对胸腺、脾脏器官指数的影响，对S180和H22荷瘤小鼠癌细胞膜和红细胞膜表面唾液酸(SA)含量的影响。结果 青龙衣冷、热醇提取部位对S180荷瘤小鼠均有明显的抑瘤作用，并呈良好的剂量效应关系；对H22荷瘤小鼠可延长生存期；冷、热醇提取部位能显著降低S180和H22小鼠肿瘤细胞膜表面SA含量，同时显著提高荷瘤小鼠红细胞膜表面SA含量。结论 青龙衣冷、热醇提取部位具有抗肿瘤作用。     

自发性高血压大鼠组织和心肾缺血组织中Na~＋／Ca~（2＋）交换体基因的表达

自发性高血压和心肾缺血组织中钙离子水平明显升高。为探讨Ｎａ＋／Ｃａ２＋交换体在其中的作用，我们应用半定量逆转录一多聚酶链反应（ＲＴ一ＰＣＲ）技术，比较了自发性高血压大鼠（ＳＨＲ）部分组织、缺血时心肌和肾脏与其正常对照组织中Ｎａ＋／Ｃａ２＋交换体ｍＲＮＡ的水平。结果表明，ＳＨＲ所测组织中Ｎａ＋／Ｃａ２＋交换体ｍＲＮＡ较正常ＷＫＹ大鼠均降低。结扎４８小时引起的缺血肾脏Ｎａ＋／Ｃａ２＋交换体ｍＲＮＡ亦降低。而因异丙基肾上腺素引起的心肌缺血组织中，Ｎａ＋／Ｃａ２＋交换体基因的表达上升。     

青龙衣胡桃醌类成分富集

目的 以胡桃醌类成分转移率为指标,通过静态吸附和解吸附筛选最佳树脂,建立青龙衣胡桃醌类成分的富集工艺.方法 比较4种不同极性的大孔吸附树脂对青龙衣胡桃醌类成分的静态吸附与解吸性能,筛选出AB8树脂为最佳树脂,优化其工艺参数.结果 最佳工艺参数为:上样液浓度0.0760 g/ml、上样pH2.0、上样流速3BV/h,洗脱浓度70%、洗脱体积3BV、洗脱流速2BV/h,径高比1:10.工艺优化后,青龙衣中胡桃醌类成分的含量由1.026%提升到11.08%,转移率为72.08%.结论 AB8型大孔树脂能有效地富集纯化青龙衣胡桃醌类成分.     

不同年龄自发性高血压大鼠血管平滑肌细胞Ca~（2＋）转运功能障碍及某些相关因素的变化

应用不同年龄自发性高血压大鼠（ＳＨＲ）血管平滑肌原代与传代细胞（ＶＳＭＣ），观察Ｃａ２＋转运功能障碍及某些相关因素的变化。结果表明：（１）在血压未升高的ＶＳＭＣＣａ２＋内流已较同龄对照组ＷＫＹ大鼠明显增加，而Ｃａ２＋外流量较ＷＫＹ大鼠显著降低。表明ＳＨＲＶＳＭＣ膜Ｃａ２＋转运功能障碍在血压升高前就已发生，并随年龄及血压增高有加重趋势；（２）ＶＳＭＣｃＡＭＰ及钙调素（ＣａＭ）含量变化与细胞膜Ｃａ２＋转运功能障碍基本同步，提示二者异常与细胞膜Ｃａ２＋转运功能障碍有密切关系；（３）血压升高前ＳＨＲＶＳＭＣ内ＡＮＧⅡ含量与ＷＫＹ大鼠相比无明显差异，但１６周龄５ＨＲＶＳＭＣ内ＡＮＧⅡ含量明显高于其幼鼠（Ｐ＜０．００１），与血压呈正相关。提示ＶＳＭＣ内ＡＮＧⅡ在血压升高过程中可能具有一定作用。以上结果表明高血压时ＶＳＭＣ膜Ｃａ２＋转运功能障碍有明显遗传倾向，ＶＳＭＣ内ＣＡＭＰ、ＣａＭ及ＡＮＧⅡ含量异常与上述障碍密切相关。     

Calcium mediation of the pig jejunal secretory response

The involvement of Ca++ ions as secretory mediators in pig jejunal epithelia has been investigated with an in vitro system. Omission of Ca++ from the Ringer-HCO3 bathing media on both sides of the tissue had minor effects on the basal electrical activity of pig jejunal mucosa. There were only slight decreases in transepithelial potential difference and increases in conductance with Ca++ free media. Low EGTA concentrations which reversibly blocked potential difference responses to secretory agents also had minimal effects on basal electrical activity. The in vitro secretory responses to A23187, to theophylline, and to Escherichia coli heat-stable enterotoxin were all eliminated by Ca++ depletion and restored by replacing normal Ca++ concentrations in the bathing media. Dantrolene prevented the secretory response but not the potential difference increases caused by heat-stable enterotoxin and A23187, suggesting that intracellular Ca++ stores may be reservoirs of secretory signal agent. Verapamil only blocked the secretory response to heat-stable enterotoxin. Chlorpromazine had negligible effects on basal conditions, but totally blocked both the secretory response and the Ca++-dependent effects of A23187 and heat-stable enterotoxin on potential difference. The response to theophylline was only partially inhibited by chlorpromazine, implying some involvement of both cAMP and Ca++ as secretory signals for theophylline. Cytoplasmic Ca++ concentrations appear to be at least as important as cyclic nucleotides in regulating the secretory effects of pig jejunum.     

急性吗啡成瘾大鼠模型的建立

目的　研究建立急性吗啡成瘾大鼠模型及对血液离子成份的影响。方法　将 6 0只大鼠随机分成吗啡成瘾组和对照组 ,比较成瘾组和对照组大鼠的戒断症状和血液分析。结果　成瘾组纳络酮诱发戒断症状结果是(34.82± 0 .5 3)次 ,对照组的结果是 (1.0 1± 0 .6 4)次 ,统计学处理显示组间差异很显著 (P <0 .0 1)。在血液离子成份上 ,统计学处理显示组间差异不显著 (P >0 .0 5 )。结论　急性吗啡成瘾大鼠模型的建立是切实可行的     

增力祛疲口服液对游泳致疲劳大鼠骨骼肌和血液生化指标的影响

目的 :探讨增力祛疲口服液抗运动性疲劳作用的现代机制。方法 :选用游泳训练导致大鼠运动性疲劳模型 ,用生化测定和细胞化学分析的方法 ,研究大鼠骨骼肌中糖原、SDH、LDH、LPO、Ca+ + 、K+ 的含量变化以及血清睾酮 (T)、皮质醇 (C)、T/C值、SOD活性、MDA含量的变化 ,观察增力祛疲口服液对其变化的影响。结果 :与正常组比较 ,对照组大鼠骨骼肌组织糖原含量、Ca+ + 、K+ 含量和血清T含量、SOD含量及T/C比值显著降低 ,而骨骼肌组织中LDH活性增强、LPO含量增加 ,血清中MDA和C含量显著增加 ,(P <0 .0 5 ) ;与对照组比较 ,实验组大鼠上述指标均显著改善。结论 :该药有利于骨骼肌组织疲劳后的功能恢复 ,有增强抗疲劳的良好作用 ,其机理与改善骨骼肌的新陈代谢和提高清除自由基的能力有关。     

Verapamil does not block the spastic response of Praziquantel on the larvae of Taenia pisiformis.

This paper describes that the previous addition of Verapamil does not block the spastic response to Praziquantel (Pz) in larvae of Taenia pisiformis kept in vitro, where opposite results were found in the literature using mammalian tissue. It is possible that Pz stimulates other Ca++ transport channels not sensitive to Verapamil action and promotes Ca++ liberation from calcareous corpuscles stimulating phospholipase C of the tegument surface generating inositol triphosphate. These hypotheses require experimental approaches to define the exact mechanism of action.     

家兔化学裸露心肌标本制备及其张力-pCa关系

家兔右室乳头肌在含3mM EDTA、5mM Na_2ATP、10mM Tris、140 mM KCl的溶液中浸浴150min,其Ca~(2+)通透性显著增高。Ca~(2+)浓度为10~(-7)M时,便可产生张力,10~(-4.6)M时,张力达到最大。相对张力-pCa(Ca~(2+)浓度的负对数)关系近似一S形曲线,产生50％最大张力的Ca~(2+)(pCa_(50))约为10~(-6.6)M。同法制备的大鼠乳头肌Ca~(2+)通透性未见增高。     

The QT interval and serum ionized calcium

The correlation between serum ionized calcium (Ca++) levels and three ECG QT intervals (Q-OTC, Q-ATC, and Q-ETC) was assessed in 20 adult patients. The relationship between each QT interval and Ca++ level, based on 209 Ca++ determinations through a range of 1.0 to 4.0 mEq/liter, is best described by a hyperbolic function. Although Q-OTC and Q-ATC predict Ca++ levels more accurately than Q-ETC, all QT intervals are clinically unreliable as guides to the presence of hypercalcemia. Similarly, the usefulness of the QT intervals in the diagnosis of hypocalcemia is limited by the wide distribution of normal values.     

云台山矿泉水水质研究

广东省云台山矿泉来自深层地下水,每日流量近300吨.泉水成分。流量 温度都十分稳定,不受季节与气候条件的影响.矿泉水主要含有氡碳酸钙,此外还含有人体必需的16种微量元素和12种稀土元素.矿泉水的主要成份为:Rn3.077ME.HCO_3-85.4mg/L、SiO_212.0mg/L.Ca~(++)30.0mg/L、Na~+14.0mg/L、K~+12.5mg/L、Mg~(++)2.30mg/L、Li~+0.01mg/L,水的pH为7.0。     

Comparison of effect of granules and herbs of Bu-Shen-Yi-Qi-Tang on airway inflammation in asthmatic mice

Background Bu-Shen-Yi-Qi-Tang （BSYQT）,which is prescribed on the basis of clinical experience,is commonly used in clinics of traditional Chinese medicine （TCM） for asthma treatment.The components of BSYQT include Radix Astragali （RA）,Herba Epimedii （HE） and Radix Rehmanniae （RR）.The aim of this study was to compare the effect of granules and herbs of BSYQT on airway inflammation in asthmatic mice.Methods Sixty female BALB/c mice were randomly divided into the normal control （NC） group,asthmatic group （A）,decoction of granules of BSYQT treatment group （GD）,decoction of herbs of BSYQT treatment group （HD）,and dexamethasone treatment group （DEX）.The mouse asthmatic model was induced by ovalbumin （OVA） sensitization and challenge.GD and HD of BSYQT as well as DEX were prepared and administered by intragastric infusion.Airway hyperresponsiveness （AHR） to methacholine （Mch）,lung histopathology analysis,inflammatory mediators in serum （IL-4,IL-5,IL-17A,IFN-γ,and eotaxin） and in lung （IL-4,IL-5,IFN-γ,and eotaxin） were selected for investigation and comparison.Results Both GD and HD treatment could decrease airway resistance （RL） and increase dynamic compliance （Cdyn） to Mch compared with the A group （P ＜0.05）.HD treatment was more effective in RL reduction than Mch at doses of 3.125 and 6.25 mg/ml （P ＜0.05） and in Cdyn increase at Mch doses of 6.25 and 12.5 mg/ml （P ＜0.05）.There were no marked differences in RL reduction and Cdyn improvement between mice in HD and DEX groups （P ＞0.05）.Both GD and HD treatment markedly attenuated lung inflammation （P ＜0.05）,and HD treatment demonstrated more significant therapeutic function in alleviating lung inflammation than that of GD and DEX treatment （P ＜0.05）.Both GD and HD treatment resulted in a significant reduction in IL-4 and IL-17A levels and an increase in the IFN-γ level in serum compared with the A group （P ＜0.05）.The effect of HD in lowering the IL-4 and IL-17A     

低钾型周期性瘫痪患者血钾水平与肢体瘫痪和补钾治疗的关系

目的研究低钾型周期性瘫痪（HoPP）患者血清钾离子浓度与肢体瘫痪程度的关系及对补钾治疗的影响.方法对95例HoPP患者的临床资料进行回顾性研究.结果 HoPP患者多表现为四肢弛缓性瘫痪,以双下肢及肢体近端为重,补钾治疗效果显著,但血钾降低水平与肢体瘫痪程度不平行,且各血钾水平组患者补钾治疗后平均治愈时间相似,经统计学处理P＞0.05,无明显差异.结论血钾水平与HoPP患者肢体瘫痪程度无明显相关性,对补钾治疗也无明显影响.