水芹总酚酸胶囊质量控制方法的研究

目的初步建立水芹总酚酸胶囊的质量控制标准。方法采用薄层色谱法对水芹总酚酸进行定性鉴别;采用Folin酚法测定水芹总酚酸含量;采用高效液相色谱法测定总酚酸中绿原酸含量,色谱条件：DiamonsilC18柱,流动相：A：乙腈-甲醇（10∶1）;B：0.4%磷酸,梯度洗脱;检测波长：325nm;流速：1ml/min;柱温：25℃。结果按现有生产工艺,水芹总酚酸胶囊总酚酸含量最高为32.42%,绿原酸最高为9.3%。结论 Folin酚法和高效液相色谱法能精确测定水芹总酚酸胶囊中总酚酸和绿原酸含量,可作为水芹总酚酸胶囊的质量控制方法 。     

连花解毒颗粒质量标准研究

目的 建立连花解毒颗粒的质量标准.方法 采用薄层色谱法对连花解毒颗粒中金银花、牛蒡子及芦根等药材进行定性鉴别.采用高效液相色谱法测定连花解毒颗粒中绿原酸和牛蒡苷的含量.结果 定性鉴别分离度好,专属性强,阴性样品无干扰.绿原酸和牛蒡苷的含量测定线性范围分别为10.4～104 μg/ml,r=0.9999、11.1～222...     

高效液相色谱法测定通关藤药材中绿原酸的含量

目的 建立通关藤药材中绿原酸的含量测定方法.方法 采用高效液相色谱法测定绿原酸的含量[分析柱Agilent ZORBAX SB C18(5μm,4.6 mm×250 mm)],流动相:乙腈-水-冰醋酸(8:92:1),检测波长:327 nm.结果 绿原酸进样浓度在8.01～32.03μg/ml范围内线性关系良好(r=0...     

高效液相色谱法测定水芹中金丝桃苷的含量

目的测定水芹中金丝桃苷的含量。方法采用高效液相色谱法,色谱柱为DiamonsilC18柱(150mm×4.6mm,5μm);乙腈甲醇(10∶1)0.4%磷酸梯度洗脱为流动相;检测波长360nm;流速1.0ml/min。结果金丝桃苷在0.0419～0.2094μg范围内呈良好线性关系,r=0.9993。平均回收率为99.2%,RSD为1.35%。结论本法简便、准确、重现性好,为水芹药材和提取物提供质量控制依据。     

高效液相色谱法测定不同产地茵陈中绿原酸的含量

目的采用高效液相色谱法测定不同产地茵陈中绿原酸的含量,考察不同产地的茵陈的质量。方法采用高效液相色谱法,色谱柱为SunFire^TM C18柱（4．6mm×150μm,5μm）;检测波长为327nm;流动相为乙腈-0．4％磷酸溶液（13：87）;流速为1ml／min,柱温为室温。结果本实验测定采集时间为2006年3月份产地为山西苛岚、河北承德、山东胶南的茵陈中绿原酸含量分别为0．320％、0．280％、0．297％,并且测定绿原酸含量的线性范围为0．1～0．6μg,r=0．9997（n=6）,平均加样回收率为96.02％,RSD为0．28％。结论不同产地的茵陈中绿原酸的含量存在差异;该方法简便、准确、重现性好,可作为茵陈中绿原酸的含量测定方法。     

HPLC法测定清咽口服液中绿原酸的含量

目的建立高效液相色谱法测定清咽口服液中绿原酸含量的方法。方法采用HPLC法,色谱柱：Wondasi C18（150mm×4.6mm,5μm）柱;流动相：甲醇-水-醋酸（20∶80∶1）;检测波长：327nm;流速：1.0mL/min。结果绿原酸在0.074～1.184μg间有良好的线性关系（r=0.999 9）,平均回收率为99.30%,RSD为1.17%（n=5）。结论本方法简便、准确、灵敏、重复性好,可用于清咽口服液的质量控制。     

茵陈五苓丸的质量标准研究

目的建立茵陈五苓丸的质量控制方法。方法采用显微鉴别、薄层色谱法对方中猪苓、茵陈、泽泻进行鉴别;采用高效液相色谱法测定绿原酸的含量。色谱条件：色谱柱为CAPCELL PAK C18（250mm×4.6mm,5μm）;流动相：乙腈-0.4%磷酸溶液（8：92）;检测波长：327nm;流速：1.0mL/min;柱温：40℃。结果采用显微鉴别和薄层色谱法鉴别方中猪苓、茵陈、泽泻。绿原酸在2.232～44.64mg·mL^-1范围内呈良好的线性关系（r=0.9999）,平均回收率为97.45%,RSD为0.68%（n=6）。结论本方法操作简单、准确、重复性好,可用于茵陈五苓丸的质量控制方法。     

高效液相色谱法考察胰岛素在能量合剂中的稳定性

目的：考察室温下8h以内胰岛素在输液用能量合剂中的稳定性情况。方法：采用高效液相色谱法，结合外观与pH值的观察，以高效液相色谱法来定量表达胰岛素的含量变化情况。结果：能量合剂在室温8h以内无色澄明，pH值较稳定。胰岛素的含量变化〈5．O％。结论：室温8h以内，胰岛素在能量合剂配伍中稳定。     

高效液相色谱法测定柴银口服液中绿原酸含量

目的 建立柴银口服液中绿原酸的含量测定方法.方法 采用高效液相色谱法,以为C18色谱柱；以甲醇-水-冰醋酸-三乙胺(15∶85∶1∶0.3)为流动相,检测波长为324nm；流速:1.0 ml/min.结果绿原酸在23.4～304.2μg峭范围内线性关系良好,平均回收率为99.84%,RSD为1.88%.结论 该法简便、...     

咽炎糖浆质量标准研究

目的制定咽炎糖浆质量标准。方法采用薄层色谱法对咽炎糖浆中金银花、玄参定性鉴别;高效液相色谱法测定绿原酸的含量,采用Symm etry C18色谱柱（3.9 mm×150 mm,5μm）,以乙腈-水-醋酸（25∶225∶5）为流动相;流速：1.0m l/min;检测波长：327 nm。结果金银花与玄参鉴别斑点清晰,分离良好。绿原酸对照品在0.1081.080μg范围内与峰面积值呈良好的线性关系。r=0.9998（n=5）,平均加样回收率为98.02%,RSD为1.36%。结论方法简便、准确、重复性好,能很好地控制咽炎糖浆的质量。     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.