EXPRESSION OF T CELL RECEPTOR V _α GENE FAMILIES IN INTRATHYROIDAL T CELLS OF CHINESE PATIENTS WITH GRAVES’ DISEASE

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ｇｒａｖｅｓ’ｄｉｓｅａｓｅ(ＧＤ)ｉｓｏｎｅｏｆｔｈｅａｕｔｏｉｍｍｕｎｅｔｈｙｒｏｉｄｄｉｓｅａｓｅｓ(ＡＩＴＤ) ,ａｎｄｉｓｃｈａｒａｃｔｅｒｉｚｅｄｂｙｈｙｐｅｒｍｅｔａｂｏｌｉｃａｎｄｈｙｐｅｒｔｈｙｒｏｉｄｓｙｎｄｒｏｍｅｃａｕｓｅｄｂｙＴＳＨｒｅｃｅｐｔｏｒ ｓｔｉｍｕｌａｔｉｎｇａｎｔｉｂｏｄｙ ＴｈｅｅｔｉｏｌｏｇｙａｎｄｐａｔｈｏｇｅｎｅｓｉｓｏｆＧＤａｒｅｓｔｉｌｌｎｏｔｆｕｌｌｙｃｌａｒｉｆｉｅｄ ＴｈｅｒｏｌｅｏｆＴｃｅｌｌｓｗａｓｗｉｄｅｌｙｓｔ…     

Relationship between the Mutation of IRS-1 Gene and β_3-adrenergic Receptor Gene

Ｏｂｅｓｉｔｙｉｓａｓｓｏｃｉａｔｅｄｗｉｔｈｉｎｓｕｌｉｎｒｅｓｉｓ ｔａｎｃｅ (ＩＲ) ,ｗｈｉｃｈｐｌａｙｓａｃｒｉｔｉｃａｌｒｏｌｅｉｎｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｔｙｐｅ 2ｄｉａｂｅｔｅｓｍｅｌｌｉｔｕｓ.ＴｈｅｍｅｃｈａｎｉｓｍｏｆＩＲｉｓｒｅｌａｔｅｄｔｏｔｈｅａｂｎｏｒｍａｌｉｔｉｅｓｏｆｉｎｓｕｌｉｎｒｅｃｅｐｔｏｒ,ｐｒｅ ｒｅｃｅｐｔｏｒ,ｐｏｓｔ ｒｅｃｅｐ ｔｏｒ ,ｒｅｓｕｌｔｉｎｇｉｎｄｅｆｅｃｔｉｖｅｍｅｔａｂｏｌｉｃｐａｔｈｗａｙｏｆｇｌｕｃｏｓｅ,ｌｉｐｉｄａｎｄ ｐｒ…     

Autoradiographic Analysis of β-Adrenoceptors in Endomyocardial Biopsy Samples

ＡｕｔｏｒａｄｉｏｇｒａｐｈｉｃＡｎａｌｙｓｉｓｏｆβ－ＡｄｒｅｎｏｃｅｐｔｏｒｓｉｎＥｎｄｏｍｙｏｃａｒｄｉａｌＢｉｏｐｓｙＳａｍｐｌｅｓＺｈａｏＭｉｎｇｍｉｎｇ（赵明明）ＭａＷｅｎｚｈｕ（马文珠）Ｚｈａｎｇｊｉｎａｎ（张寄南）ＷａｎｇＪｉｎｇｌｉ...     

Relationship between bone mineral density and polymorphism of the estrogen receptor gene in healthy postmenopausal women in China

Ｏｂｊｅｃｔｉｖｅ　Ｔｏｉｎｖｅｓｔｉｇａｔｅｔｈｅｐｏｓｓｉｂｌｅｒｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｂｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙａｎｄｐｏｌｙｍｏｒｐｈｉｓｍｏｆｔｈｅｅｓｔｒｏｇｅｎｒｅｃｅｐｔｏｒ（ＥＲ）ｇｅｎｅｉｎＳｈａｎｇｈａｉｈｅａｌｔｈｙｐｏｓｔｍｅｎｏｐａｕｓａｌｗｏｍｅｎＭｅｔｈｏｄｓ　２５０ｕｎｒｅｌａｔｅｄｈｅａｌｔｈｙｐｏｓｔｍｅｎｏｐａｕｓａｌｗｏｍｅｎｗｅｒｅｓｅｌｅｃｔｅｄｆｏｒｂｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙ（ＢＭＤ）ｄｅｔｅｒｍｉｎａｔｉｏｎｂ…     

Agonist-directed sorting of β_2-adrenergic receptor signaling and its therapeutic implication in heart

ＰｒｏｍｉｓｃｕｏｕｓｃｏｕｐｌｉｎｇｏｆａＧ ｐｒｏｔｅｉｎｃｏｕｐｌｅｄｒｅｃｅｐ ｔｏｒｔｏｍｏｒｅｔｈａｎｏｎｅｃｌａｓｓｏｆＧｐｒｏｔｅｉｎｓｈａｓｒｅｃｅｎｔｌｙｂｅｅｎｄｅｍｏｎｓｔｒａｔｅｄｉｎａｒｔｉｆｉｃｉａｌｓｙｓｔｅｍｓ ,ｉｎｃｌｕｄｉｎｇｌｉｐｉｄｖｅｓｉｃｌｅｓａｎｄｔｒａｎｓｆｅｃｔｅｄｃｅｌｌｌｉｎｅｓ[1,2 ] ,ａｎｄｉｎｎａｔｉｖｅｔｉｓｓｕｅｓａｎｄｃｅｌｌｓ[3～ 5] .Ａｆｕｎｄａｍｅｎｔａｌｑｕｅｓｔｉｏｎａｒｉｓｅｎｉｓｈｏｗｔｈｅｒｅ ｃｅｐｔｏｒｓｔｉｍｕｌ…     

Gene transfer of a β2-adrenergic receptor kinase inhibitor up-regulates the level of β2-adrenergic receptor and cAMP in the asthmatic murine lung

Objective: To investigate the effects of gene transfer of a β-adrenergic receptor(β-AR) kinase inhibitor(β ARKct)on pulmonary β2-adrenergic receptor and cAMP following β2-AR agonist treatment in asthmatic mice, and to analyze the relationship between the routes of gene delivery and the changes of β2AR and cAMP. Methods: BALB/c mice were sensitized and challenged by ovalbumin to establish the asthmatic model treated with βAR agonist ( salbutamol injected intramuscularly). The plasmid with the expression of βARKct was constructed and βARKct gene transfer was performed through intravenous injection or intratracheal instillation in asthmatic mice.The gene expression was measured with Western blot analysis, and the changes of pulmonary β-AR and cAMP evaluated by Radioimmunoassay. Results: The expression of tranfered βARKct gene was detectable in lungs and it was expressed more in the lungs of the mice receiving intratracheally plasmid than those receiving intravenously. The levels of βAR and cAMP were upregulated after using plasmid-βARKct to the asthmatic mice treated with β AR agonist. Conclusion: Our results indicated that there were down-regulation of βAR and cAMP in asthmatic mice treated with βAR agonist. Gene transfer of βARKct could inhibit the extent of the down-regulation of βAR and cAMP. The route of gene delivery could also affect the degree of up-regulation of βAR and cAMP. Gene transfer βARKct may provide a novel approach to the therapeutic strategy for asthma.     

Expression of Human Vascular Endothelial Growth Factor Receptor Flt-1 Extracellular Domain 1-3 Loop cDNA in Pichia pastoris, Purification of the Expressed Product and Detection of Its Biological Activity

ＩＮＴＲＯＤＵＣＴＩＯＮＶａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ (ＶＥＧＦ)ｒｅｃｅｐｔｏｒＦｌｔ 1ｉｓａｋｉｎｄｏｆｇｌｕｃｏｐｒｏｔｅｉｎｗｉｔｈａｍｏｌｅｃｕｌａｒｗｅｉｇｈｔｏｆ 1 80ＫＤ .Ａｓａｆａｍｉｌｙｍｅｍｂｅｒｏｆｔｙｒｏｓｉｎｅｐｒｏｔｅｉｎｋｉｎａｓｅｒｅｃｅｐｔｏｒｓｕｂ ｔｙｐｅⅢ ,ｉｔｃｏｎｓｉｓｔｓｏｆｅｘｔｒａｃｅｌｌｕｌａｒ,ｔｒａｎｓｍｅｍｂｒａｎｅａｎｄｉｎｔｒａｃｅｌｌｕｌａｒｄｏｍａｉｎｓ.ＴｈｅｒｅａｒｅｓｅｖｅｎＩｇ ｌｉｋｅｌｏｏｐ…     

Modulation by Divalent Cations of GABAρ1 Receptor From Human Retina Expressed in Xenopus Oocytes

ＩＮＴＲＯＤＵＣＴＩＯＮγ ａｍｉｎｏｂｕｔｙｒｉｃａｃｉｄ (ＧＡＢＡ)ｆｕｎｃｔｉｏｎｓａｓａｎｉｎｈｉｂｉｔｏｒｙｎｅｕｒｏｔｒａｎｓｍｉｔｔｅｒｉｎｔｈｅｍａｍ ｍａｌｉａｎｃｅｎｔｒａｌｎｅｒｖｏｕｓｓｙｓｔｅｍ .Ｉｎｉｔｉａｌｌｙ,ＧＡＢＡｒｅｃｅｐｔｏｒｓｗｅｒｅｄｉｖｉｄｅｄｉｎｔｏｔｗｏｗｅｌｌ ｃｈａｒａｃ ｔｅｒｉｚｅｄｒｅｃｅｐｔｏｒｔｙｐｅｓ,ＧＡＢＡＡａｎｄＧＡＢＡＢｒｅｃｅｐｔｏｒｓ.Ａｔｈｉｒｄｔｙｐｅｗａｓｄｉｓｃｏｖｅｒｅｄｉｎｂｒａｉｎａｎｄｒｅｔｉｎａ,ａｎｄｗａｓ…     

Vitamin D receptor gene polymorphism in postmenopausal women of the Han and Uygur nationalities in China

Ｔｈｅｒｉｓｋｏｆｏｓｔｅｏｐｏｒｏｓｉｓｉｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｂｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙ (ＢＭＤ ) ,ｗｈｉｃｈｉｓｕｎｄｅｒｓｔｒｏｎｇｇｅｎｅｔｉｃｃｏｎｔｒｏｌ Ｍｏｒｒｉｓｏｎｅｔａｌ1　ｈａｖｅｓｈｏｗｎａｓｔｒｏｎｇｒｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｐｏｌｙｍｏｒｐｈｉｓｍａｔｔｈｅｖｉｔａｍｉｎＤｒｅｃｅｐｔｏｒ (ＶＤＲ)ｇｅｎｅａｎｄＢＭＤ ＩｎａｄｄｉｔｉｏｎｔｏＢｓｍⅠ ,ｒｅｓｔｒｉｃｔｉｏｎｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒｐｈｉｓｍａｔｔｈ…     

P2Z purinoceptor, a special receptor for apoptosis induced by ATP in human leukemic lymphocytes

ＯｂｊｅｃｔｉｖｅＴｏｉｎｖｅｓｔｉｇａｔｅｔｈｅｒｏｌｅｏｆｐｕｒｉｎｅｒｇｉｃＰ２Ｚｒｅｃｅｐｔｏｒｓｆｏｒａｐｏｐｔｏｓｉｓｏｆｈｕｍａｎｌｅｕｋｅｍｉｃｌｙｍｐｈｏｃｙｔｅｓｍｅｄｉａｔｅｄｂｙｅｘｔｒａｃｅｌｕｌａｒａｄｅｎｏｓｉｎｅｔｒｉ...     

Clinical Measurement of Antibodies against Acetylcholine Receptor（AchR），SOD and LPO in Patients with Myasthenia Gravis（MG） be

ＣｌｉｎｉｃａｌＭｅａｓｕｒｅｍｅｎｔｏｆＡｎｔｉｂｏｄｉｅｓａｇａｉｎｓｔＡｃｅｔｙｌｃｈｏｌｉｎｅＲｅｃｅｐｔｏｒ（ＡｃｈＲ），ＳＯＤａｎｄＬＰＯｉｎＰａｔｉｅｎｔｓｗｉｔｈＭｙａｓｔｈｅｎｉａＧｒａｖｉｓ（ＭＧ）ｂｅｆｏｒｅａｎｄａｆｔｅｒＴｈ...     

Evaluation of cardiac beta-adrenergic receptor function in children by dobutamine stress echocardiography

ＯｂｊｅｃｔｉｖｅＴｏｉｎｖｅｓｔｉｇａｔｅｔｈｅｒｏｌｅｏｆｄｏｂｕｔａｍｉｎｅｓｔｒｅｅｅｃｈｏｃａｒｄｉｏｇｒａｐｈｙｉｎｅｖａｌｕａｔｉｎｇｃａｒｄｉａｃｂｅｔａａｄｒｅｎｅｒｇｉｃｒｅｃｅｐｔｏｒ（βＡＲ）ｆｕｎｃｔｉｏｎａｎｄｒｅｓｐ...     

Signaling-selective β_2-adrenergic stimulation:therapeutic implications in chronic heart failure

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ａｐｐｒｏｘｉｍａｔｅｌｙ 5ｍｉｌｌｉｏｎＡｍｅｒｉｃａｎｓｈａｖｅｃｈｒｏｎｉｃｈｅａｒｔｆａｉｌｕｒｅ(ＨＦ) .Ｉｔｉｓｔｈｅｍｏｓｔｃｏｍｍｏｎｃａｕｓｅｏｆｈｏｓｐｉ ｔａｌｉｚａｔｉｏｎａｍｏｎｇＭｅｄｉｃａｒｅｐａｔｉｅｎｔｓ ,ａｔａｎａｎｎｕａｌｃｏｓｔｏｆａｐｐｒｏｘｉｍａｔｅｌｙ＄ 12ｂｉｌｌｉｏｎｄｏｌｌａｒｓ[1,2 ] .Ｄｅｓｐｉｔｅｒｅｃｅｎｔｔｈｅｒａｐｅｕｔｉｃａｄｖａｎｃｅｓ ,ｔｈｅｓｉｘｙｅａｒｍｏｒｔａｌｉｔｙｒａｔｅｉｎｐａｔｉｅｎｔｓｗｉ…     

DYNAMIC CHANGES OF SERUM VASCULAR ENDOTHELIAL GROWTH FACTOR LEVELS IN A RAT MYOCARDIAL INFARCTION MODEL

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ(ＶＥＧＦ) ,ａｈｏｍｏｄ ｉｍｅｒｉｃ 34 ｔｏ 46 ｋＤａｈｅｐａｒｉｎ ｂｉｎｄｉｎｇｇｌｙｃｏｐｒｏｔｅｉｎ ,ｉｓａｐｏ ｔｅｎｔｓｔｉｍｕｌａｔｏｒｏｆｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｒｅｐｌｉｃａｔｉｏｎａｎｄｉｓｒｅｇａｒｄｅｄａｓｏｎｅｏｆｔｈｅｍｏｓｔｓｐｅｃｉｆｉｃｏｆｔｈｅｋｎｏｗｎａｎｇｉｏｇｅｎｉｃｍｅｄｉａｔｏｒｓｂｅｃａｕｓｅｔｈｅｌｏｃａｌｉｚａｔｉｏｎｏｆｉｔｓｒｅｃｅｐｔｏｒｓｉ…     

Acting via A_2 receptors，adenosine inhibits H_2O_2 production and elastase releaseon FMLP一stimulated

ＡｃｔｉｎｇｖｉａＡ２ｒｅｃｅｐｔｏｒｓ，ａｄｅｎｏｓｉｎｅｉｎｈｉｂｉｔｓＨ２Ｏ２ｐｒｏｄｕｃｔｉｏｎａｎｄｅｌａｓｔａｓｅｒｅｌｅａｓｅｏｎＦＭＬＰ一ｓｔｉｍｕｌａｔｅｄｎｅｕｔｒｏｐｈｉｌｓＺｈａｎｇＹｕ，ＢａＴｕ（ＤｅｐａｒｔｍｅｎｔｏｆＣａ...     

Effect of 1,25(OH)_2D_3 on the growth and apoptosis of breast cancer cell line MCF-7

Ｔｈｅｓｔｅｒｏｉｄｈｏｒｍｏｎｅ 1,2 5 ｄｉｈｙｄｒｏｘｙｖｉｔａｍｉｎＤ3 (1,2 5(ＯＨ) 2 Ｄ3 )ｉｓｂｉｏｌｏｇｉｃａｌｌｙｔｈｅｍｏｓｔａｃｔｉｖｅｆｏｒｍｏｆｖｉｔａｍｉｎＤ3 ａｎｄｐｌａｙｓａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｔｈｅｒｅｇｕｌａｔｉｏｎｏｆｃａｌｃｉｕｍａｎｄｂｏｎｅｍｅｔａｂｏｌｉｓｍ Ｔｈｅｅｆｆｅｃｔｓｏｆ1,2 5(ＯＨ) 2 Ｄ3 ａｒｅｍｅｄｉａｔｅｄｂｙｖｉｔａｍｉｎＤｒｅｃｅｐｔｏｒｓ (ＶＤＲ)ｉｎｔａｒｇｅｔｔｉｓｓｕｅｓ Ｉｎｔｈｅｌａｓｔｄｅｃａｄｅ,ＶＤＲｗａｓｎｏ…     

Detection of Circulating Autoantibodiestoβ_2-Adrenergic Receptors in Patients with Asthma

ＤｅｔｅｃｔｉｏｎｏｆＣｉｒｃｕｌａｔｉｎｇＡｕｔｏａｎｔｉｂｏｄｉｅｓｔｏβ２┐ＡｄｒｅｎｅｒｇｉｃＲｅｃｅｐｔｏｒｓｉｎＰａｔｉｅｎｔｓｗｉｔｈＡｓｔｈｍａＷｕＪｉａｎｑｉｎｇ（吴剑卿）ＹｉｎＫａｉｓｈｅｎｇ（殷凯生）＊ＹａｎｇＹｕ（杨玉）＊Ｄｅ...     

Multiple splice variants of mRNA for human FMS like tyrosine kinase 3 ligand from fetal liver

ＯｂｊｅｃｔｉｖｅＦＭＳｌｉｋｅｔｙｒｏｓｉｎｅｋｉｎａｓｅ３（ＦＬＴ３）／Ｆｅｔａｌｌｉｖｅｒｋｉｎａｓｅ２（ＦＬＫ２）,ＫｉｔａｎｄＦＭＳａｒｅｃｌａｓⅢｔｙｒｏｓｉｎｅｋｉｎａｓｅｒｅｃｅｐｔｏｒｓｅｘｐｒｅｓｅｄｉｎｔｈｅｈｅｍａｔｏｐｏｉｅ...     

Sex hormone receptors of hemangiomas in children.

ＳｅｘｈｏｒｍｏｎｅｒｅｃｅｐｔｏｒｓｏｆｈｅｍａｎｇｉｏｍａｓｉｎｃｈｉｌｄｒｅｎＬｉｕＷｅｎｙｉｎｇ刘文英，ＺｈａｎｇＳｈａｎｇｆｕ张尚福，ＨｕＴｉｎｇｚｅ胡廷泽，ＷｅｉＦｕｋａｎｇ韦福康ＧａｏＹａｎ高艳ａｎｄＣｈｅｎｇＮｉａｎｇ成娘Ｏｂｊｅｃｔ...     

Development of cytotoxic cerebral edema in rats following intracaudatumin jection of tACPD,an agonist of metabotropic glutamate receptors

Ｏｂｊｅｃｔｉｖｅ　Ｔｏｅｘｐｌｏｒｅｔｈｅｉｎｖｏｌｖｅｍｅｎｔｏｆｍｅｔａｂｏｔｒｏｐｉｃｇｌｕｔａｍａｔｅｒｅｃｅｐｔｏｒｓ (ｍＧｌｕＲｓ)ｉｎｔｈｅｆｏｒｍａｔｉｏｎｏｆｃｅｒｅｂｒａｌｅｄｅｍａ Ｍｅｔｈｏｄｓ　ＭａｌｅＷｉｓｔａｒｒａｔｓｗｅｉｇｈｉｎｇｆｒｏｍ 2 50ｇｔｏ 30 0ｇｗｅｒｅｕｓｅｄ Ｔｒａｎｓ 1 ａｍｉｎｏｃｙｃｌｏｐｅｎｔａｎｅ 1,3 ｄｉａｃａｒｂｏｘｙｌｉｃａｃｉｄ (ｔＡＣＰＤ) ,ａｎａｇｏｎｉｓｔｏｆｍＧｌｕＲｓ ,ｗａｓｍｉｃｒｏｉｎｊｅｃｔｅｄｉｎｔｏｔｈｅｒｉｇｈｔ…