冠心病患者血清肿瘤坏死因子、白细胞介素-6水平及其关系

目的 :观察冠心病 （CHD）患者血清肿瘤坏死因子 （TNF）、白细胞介素 - 6 （IL- 6 ）的变化与临床意义。方法 :采用放射免疫分析法 （RIA）测定 45例 CHD患者及 30例健康者 （对照组 ）血清 TNF,IL- 6水平。结果 :CHD患者血清TNF,IL- 6水平均显著高于对照组 （P<0 .0 1） ,尤以心肌梗死患者为明显 （P<0 .0 1） ;心肌梗死组血清 TNF,IL- 6水平较心肌缺血组显著升高 （P<0 .0 1） ;急性心肌梗死患者血清 TNF,IL- 6水平高于陈旧性心肌梗死患者 （P<0 .0 5 ） ;心肌缺血组心功能 ～ 级患者血清 TNF,IL- 6水平较心功能 ～ 级患者升高显著 （P<0 .0 1） ;血清TNF水平与 IL- 6之间呈明显正相关 （r=0 .73,P<0 .0 1）。结论 :血清 TNF,IL- 6水平与 CHD病情严重程度密切相关 ,TNF,IL- 6可能参与 CHD的发生与发展。     

肿瘤坏死因子-α、白细胞介素-6、白细胞介素-8与肝纤维化的关系

目的　研究肿瘤坏死因子- α(TNF- α)、白细胞介素6(IL- 6),白细胞介素8(IL -8)与肝纤维化的关系。方法　采用放射免疫分析法测定1999-01~2000-12沈阳市传染病院203例慢性肝病患者血清TNF- a、IL -8、IL- 6水平,并用电子胃镜检查了其中64例患者的食管静脉情况。结果　慢性肝病患者血清TNF- a、IL- 6、IL -8、透明质酸(HA)、层连蛋白(LN)、IV胶原C(IV- C)水平较正常对照组均明显升高(P<0. 05)。TNF -α、IL- 6、IL -8与HA、LN、IV -C水平间均呈显著正相关(P<0 .01),与血清总胆红素(TBiL)、凝血酶原活动度(PTA)及白、球蛋白比值(A/G)均密切相关(P<0. 01 )。食管静脉曲张患者血清TNF- α、IL- 6、IL -8水平较无食管静脉曲张患者升高,但现两组间差异无显著性(P>0 .05)。结论　提示TNF- α、IL -6、IL- 8与肝纤维化密切相关,并可能反映肝脏损害程度。     

大黄对老年冠心病患者肿瘤坏死因子-α和白细胞介素-6水平的影响

目的观察大黄对老年冠心病患者(CHD)血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)表达水平的影响。方法收集40例老年CHD患者,随机分为大黄干预组(21例)和常规治疗组(19例)。常规治疗组给予常规治疗,大黄干预组在常规治疗基础上加用中药大黄,观察2个月。另有体检正常的17例为健康对照组纳入研究。采用ELISA法检测各组治疗前后血清TNF-α、IL-6的表达水平,并对治疗后两组临床疗效进行比较。结果两组患者治疗前TNF-α、IL-6表达水平显著高于健康体检者。治疗后大黄干预组和常规治疗组血清TNF-α、IL-6含量较治疗前显著降低(P0.01),大黄干预组治疗后与常规治疗组比较差异有显著统计学意义(P0.01),且临床疗效优于常规治疗组(P0.05)。结论大黄能够有效抑制老年冠心病患者血清TNF-α、IL-6的表达水平,而且临床疗效好。     

心绞痛患者血浆白细胞介素-6和肿瘤坏死因子-α的变化及临床意义

目的 研究心绞痛患者白细胞介素-6（IL-6）及肿瘤坏死因子-α（TNF-α）的变化趋势及临床意义.方法 纳入冠状动脉造影确诊的心绞痛患者70例,包括稳定型心绞痛（SAP）组34例,不稳定型心绞痛（UAP）组36例,另外纳入30例虽有不典型心绞痛症状但冠状动脉造影正常者作为对照组.采用酶联免疫吸附法检测各组血浆IL-6、TNF-α水平.结果 对照组、SAP组和UAP组血浆IL-6和TNF-α水平均呈逐渐增加的趋势,IL-6的表达水平分别为：（11.64±2.49）pg/L、（14.79±2.53）pg/L、（18.87±2.58）pg/L;TNF-α的表达水平分别为：（16.08±4.23）ng/L、（19.87±4.43）ng/L和（22.91±4.51）ng/L,组间差异均有统计学意义（P均＜0.01）.结论 血浆IL-6与TNF-α在反映冠状动脉斑块稳定性及冠状动脉病变的严重程度方面有重要的参考意义.     

肿瘤坏死因子-α、白细胞介素-1 β、-6水平与充血性心力衰竭关系的临床研究

目的探讨肿瘤坏死因子α(TNFα)、白细胞介素1β(IL1β)、白细胞介素6(IL6)与充血性心力衰竭(CHF)的关系。方法采用放射免疫分析法(RIA)测定三组入选者血清TNFα、IL1β、IL6的含量,CHF心功能Ⅰ、Ⅱ级组(30例),CHF心功能Ⅲ、Ⅳ级组(30例),对照组(28例)。结果心功能Ⅲ、Ⅳ级组患者血清TNFα、IL1β、IL6含量显著高于对照组(P<0.01)和心功能Ⅰ、Ⅱ级组(P<0.05),心功能Ⅰ、Ⅱ级组显著高于对照组(P<0.01);TNFα与IL1β、IL6呈显著正相关(r1=0.763,r2=0.684均P<0.01)。结论TNFα、IL1β、IL6水平与CHF发生发展密切有关。     

肿瘤坏死因子-α、白细胞介素-6与溃疡性结肠炎的关系

部分研究显示细胞因子改变在溃疡性结肠炎(UC)的发生机制中可能起重要作用.不同的细胞因子产物与基因启动子、信号序列、基因内含子中的单核苷酸多态现象有关.目的:测定UC患者肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的基因多态性及其血清浓度,从而探讨TNF-α、IL-6与UC的关系.方法:以聚合酶链反应-序列特异性引物(PCR-SSP)法检测60例UC患者和60名正常对照者的TNF-α、IL-6基因型,以酶联免疫吸附测定(ELISA)检测血清TNF-α、IL-6浓度.结果:UC患者TNF-α-308、IL-6-174位点基因型频率和等位基因频率与正常对照组相比均无明显差异;UC病变位于全结肠者的TNF-α-308GG基因型频率显著高于左半结肠组和直肠组(P<0.05).UC患者血清TNF-α、IL-6浓度显著高于正常对照组(P<0.05);UC患者不同基因型间血清TNF-α和IL-6浓度相比无明显差异,而正常对照组之间差异有统计学意义(P<0.05).结论:TNF-α-308、IL-6-174位点基因多态性与UC发病的易感性无关;TNF-α-308GG基因型可能与UC病变范围有关;健康人中TNF-α、IL-6基因型是其血清水平的决定性因素,而UC患者血清TNF-α、IL-6含量可能还受其他因素的影响.     

冠状动脉斑块形态与外周血肿瘤坏死因子、内皮素和白细胞介素-6的关系

目的通过观察冠心病患者冠状动脉粥样硬化斑块形态与血清肿瘤坏死因子(tumornecrosis factor-alpha,TNF-α)、内皮素(endothelin,ET)和白细胞介素6(Interleukin-6,IL-6)的关系,探讨斑块发生破裂的原因,进而为临床上防治不稳定斑块的破裂提供理论依据。方法测定经冠状动脉造影(Coronary angiography,CAG)确诊的230例“冠心病、心绞痛”患者以及230例CAG正常者的血清TNF-α,ET和IL-6水平,并比较其与不同冠状动脉斑块形态之间的关系。结果冠心病患者血清TNF-α,ET和IL-6水平明显高于正常对照组,2型斑块组血清上述指标水平显著高于1型3、型斑块组,且3型斑块组明显高于1型斑块组。结论冠状动脉粥样硬化的过程中有炎症反应参与,炎症因子TNF-α,ET和IL-6水平与冠状动脉病变斑块形态均是导致冠状动脉斑块破裂的重要因素。     

老年脑出血患者血清肿瘤坏死因子-α、白细胞介素-6、-8与脑组织水肿相关性

目的探究老年脑出血患者血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6及IL-8与脑组织水肿之间的相关性。方法 76例老年脑出血患者中观察组41例为脑出血合并脑水肿患者,对照组35例为脑出血不伴有脑水肿患者。结果观察组血清中TNF-α在12 h~3 d出现逐渐升高的趋势,在第3天水平达到峰值,然后逐渐降低,12、24 h、3 d血清TNF-α峰明显高于对照组(P0.05)。观察组血清IL-6水平在12~24 h内逐渐上升,在24 h内达到峰值,3~7 d水平开始逐渐降低,12、24 h血清IL-6水平明显高于对照组,3~7 d血清IL-6水平则低于对照组(P0.05)。观察组血清中IL-8水平呈现逐渐升高的趋势,第7天血清中IL-8水平最高,观察组12 h~7 d血清中IL-8水平显著高于对照组(P0.05);观察组12~24 h出血量、水肿量出现升高的趋势,24 h出血量和水肿量出现最大值,随着病情发展,3~7 d出血量和水肿量呈现下降的趋势,不同发病时间之间均具有统计学意义(P0.05);Pearson相关性检验分析,观察组24 h水肿量与TNF-α(r=0.326,P=0.038)、IL-6(r=0.394,P=0.011)、IL-8(r=0.351,P=0.024)及24 h出血量(r=0.612,P0.001)呈现显著正相关。结论 TNF-α、IL-6及IL-8可能参与了老年脑出血水肿的发病机制,可作为老年脑出血水肿患者病情严重程度的监测指标。     

类风湿关节炎患者血清肿瘤坏死因子α、白细胞介素-6的检测及其临床意义

目的：探讨类风湿关节炎（RA）患者血清瘤坏死因子（TNF）α、白细胞介素-6（IL6）水平变化及其临床意义。方法；应用酶联免疫吸附法（ELISA）检测RA患者治疗前后血清TNFα、IL－6水平，并与部分临床指标作相关性分析，结果：RA患者血清TNFα、IL－6水平明显高于正常对照组，且与部分临床指标是正相关，重度活动期RA患者TNFα、IL－6水平明显高于轻、中度活动期，治疗后随着病情好转，患者血清TNFα、IL－6水平有明显下降。结论：RA的发病与血清TNFα、IL－6介导的细胞免疫调节异常有着明显的关系。检则血清TNFα、IL－6水平对RA的病情活动观察、药物的选择具有重要的临床价值。     

C-反应蛋白白细胞介素-6和肿瘤坏死因子-α在冠心病中的变化

目的 :探讨炎症标记物与冠心病的关系及临床意义。方法 :选择健康体检者 (正常对照组 ) 30例、稳定型心绞痛 (SAP组 )患者 2 0例、不稳定型心绞痛 (UAP)患者 2 5例和急性心肌梗死 (AMI)患者 30例 ,分别测定血清中C 反应蛋白 (CRP)、白细胞介素 6 (IL 6 )和肿瘤坏死因子 α(TNF α)的浓度。结果 :①SAP、UAP、AMI与正常对照组比较 ,血清中CRP、IL 6、TNF α均显著增高 ,差异均具有统计学意义 (均P <0 .0 1) ;②UAP与SAP比较 ,血清中CRP、TNF α均显著增高 (P <0 .0 1) ,但与IL 6比较差异无统计学意义 ;③AMI与SAP比较 ,血清中CRP、IL 6、TNF α均显著增高 ,差异均具有统计学意义 (P <0 .0 1) ;④AMI与UAP比较 ,血清中CRP、IL 6、TNF α均显著增高 ,差异均具有统计学意义 (P <0 .0 5 ) ;⑤血清中CRP、IL 6、TNF α在AMI发病的 72h以内有明显动态变化。结论 :炎症参与了冠心病的发病过程 ,血清中炎症因子水平的高低与冠心病的严重程度有关 ,AMI时炎症因子的动态演变可以作为病情稳定性的指标 ,对判断病情和预后具有临床价值。     

冠心病患者血清IL-1β和IL--6含量与冠脉病变的关系

目的：探讨血清自细胞介素（IL）-1β、IL--6水平对冠心病发病和严重度的预测作用。方法：70例冠心病患者，按临床诊断分为2组：急性冠脉综合症组32例和稳定型心绞痛组38例，另有对照组患者30例。ELISA检测各组患者血清中IL--1β、IL--6水平，并比较各组间的差异。冠脉造影术对冠脉病变进行Gensini评分，并了解其与血清中IL-1β、IL--6水平的相关性。结果：冠心病患者血清中的IL-1β、IL-6水平显著高于正常对照组（P〈O．05）；在冠心病组内，急性冠脉综合症组的血清IL-6水平显著高于稳定型心绞痛患者（P〈0.05）。冠脉病变Gensini评分与血清中IL-6水平呈正相关（r=0．358，P〈0．05）。结论：IL-1β、IL-6可能参与了冠状动脉粥样硬化的发生和发展过程，其血清水平可能是冠状动脉粥样硬化的-个炎性标志。     

血清IL-6、IL-8和TNF—α在重症急性胰腺炎早期诊断中的临床意义

目的 对血清白介素-6(IL-6)、白介素-8(IL-8)及肿瘤坏死因子-α(TNF-α)等细胞因子在早期诊断重症急性胰腺炎(severe acute pancreatitis，SAP)中的价值进行研究。方法 以Ranson和APACHE—Ⅱ评分为标准，把36例急性胰腺炎患者分成轻症胰腺炎组(MAP)及重症胰腺炎组(SAP)两组，选择年龄匹配的13名健康志愿者为对照组(Con)；在患者入院24h内采静脉血并检测血清中IL-6、IL-8和TNF-α水平。结果 三种细胞因子血清水平均为SAP组最高，Con组最低，其中IL-6水平在三组之间、两两之间均有显著差异，IL-8和TNF—α水平在Con组与SAP组之间、MAP组与SAP组之间有显著差异，而Con组与MAP组之间无显著差异。结论 IL-6、IL-8、TNF—α对早期诊断鉴别轻症胰腺炎和重症胰腺炎均有重要的临床意义。     

冠心病患者血清白介素-6、白介素-8和肿瘤坏死因子的变化及临床意义

目的探讨冠心病患者血清炎症因子白介素-6（IL-6）、白介素-8（L-8）和肿瘤坏死因子（TNF）含量在冠心病发展过程中的意义。方法测定81例冠心病患者血清炎症因子IL-6、IL-8和TNF含量,观察冠心病患者冠脉狭窄程度与血清IL-6、IL-8及TNF含量变化的关系。结果40例冠脉重度狭窄者血清炎症因子IL-6、IL-8和TNF分别为（163．41±64．18）pg／ml、（0．41±0．11）ng／ml、（2．54±0．63）ng／ral,41例冠脉轻中度狭窄者血清炎症因子IL-6、IL-8和TNF分别为（97．05±27．29）pg／ml、（0．34±0．16）ng／ml、（1．86±0．33）ng／ml,两组间差异存在统计学意义（P〈0．05）。结论血清炎症因子IL-6、IL-8和TNF与冠心病的严重程度有关。冠脉狭窄越明显,炎症因子IL-6、IL-8及TNF含量越高。     

心脏停跳与不停跳手术TNF-α、IL-6和IL-8的动态变化及对比研究

目的探讨先天性心脏病体外循环心脏停跳与不停跳手术围术期血浆肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的动态变化及对比研究.方法30例患者随机分成两组,分别在体外循环心脏停跳下和不停跳下行心脏手术,在5个时间点中心静脉采血,用放免法测定TNF-α、IL-6和IL-8的血浆含量.结果两组患者术前血浆TNF-α、IL-6和IL-8平均水平无明显差异,体外循环开始30 min、结束2 h及术后6 h,均比术前明显升高,术后24 h IL-8与TNF-α基本回落到术前水平,而IL-6仍保持较高水平.A组患者在体外循环结束2 h及术后6 h的TNF-α、IL-6和IL-8水平显著高于B组.结论体外循环和心肌缺血-再灌注损伤会引起患者血浆致炎因子,如TNF-α、IL-6和IL-8水平的升高;心脏不停跳手术方法因为没有心肌缺血-再灌注的过程,减少了心肌损伤,故具有良好的心肌保护作用.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.