奥拉西坦联合丁苯酞治疗卒中后血管性痴呆的效果及血清脑源性神经营养因子、同型半胱氨酸水平观察

目的 分析奥拉西坦联合丁苯酞治疗卒中后血管性痴呆（VD）的临床效果,并分析对患者血清因子指标的影响。方法 选取泰州市第三人民医院2021年1月至2023年2月收治的68例卒中后VD患者,按随机数字表法将其分为对照组（34例）和研究组（34例）。在常规治疗（营养脑神经、维持水和电解质平衡,稳定控制血糖、血脂及血压,以及康复训练）的基础上,对照组患者采用奥拉西坦胶囊进行治疗,研究组患者在对照组的基础上加用丁苯酞软胶囊进行治疗。两组患者均治疗2个月。比较两组患者治疗前后美国国立卫生研究院卒中量表（NIHSS）、Barthel指数（BI）、临床痴呆程度量表（CDR）、简易智能精神状态检查量表（MMSE）评分及脑血管功能、血清因子水平,以及治疗期间不良反应的发生情况。结果 与治疗前比,治疗后两组患者的CDR、NIHSS评分均降低,且研究组均较对照组更低;两组患者的MMSE、BI评分均升高,且研究组均较对照组更高;与治疗前比,治疗后两组患者颅脑中动脉搏动指数均降低,且研究组较对照组更低;两组患者收缩期血流速度、平均血流速度均增快,且研究组较对照组更快;与治疗前比,治疗后两组患者血清脑源性神经营养因...     

脑源性神经营养因子研究进展及在儿科的应用

阐述脑源性神经营养因子通过与其相应的受体trkB结合发挥生理作用 ,及其促进和维持神经细胞功能的生物学效应的研究 ,进一步探讨脑源性神经营养因子在儿科临床的应用。     

脑源性神经营养因子研究进展及在儿科的应用

阐述脑源性神经营养因子通过与其相应的受体trkB结合发挥生理作用，及其促进和维持神经细胞功能的生物学效应的研究，进一步探讨脑源性神经营养因子在儿科临床的应用。     

脑源性神经营养因子与新生儿缺氧缺血性脑损伤

新生儿缺氧缺血性脑损伤是人类致残的主要原因之一。虽然目前有多种方法对此疾病进行干预，但是尚未发现真正治疗此疾患的方案。越来越多的证据显示脑源性神经营养因子利于神经细胞的正常生长发育，而且能够刺激缺氧缺血性脑损伤所致受损的神经细胞康复。该文就脑源性神经营养因子对缺氧缺血性脑损伤的保护机理，动物实验疗效，临床应用的挑战及前景进行综述，为临床应用脑源性神经营养因子治疗缺氧缺血性脑损伤提供可靠的理论依据。     

脑源性神经营养因子对阿尔茨海默病的作用

脑源性神经营养因子(BDNF)是神经营养因子家族中的一员,BDNF对周围和中枢神经元有广泛的生物学作用。阿尔茨海默病(AD)是以认知功能障碍和记忆损害为特征的神经退行性疾病,是老年人中常见的一种痴呆症。目前对BDNF与AD的关系和对AD的作用的研究已成为热点。本文重点综述了近年BDNF对AD作用的研究进展。     

三磷酸胞苷对脑缺血大鼠脑源性神经营养因子的影响

目的:观察CTP对局灶脑缺血大鼠BANF表达的影响.方法:应用免疫组化方法观察不同缺血时间CTP治疗组及脑缺血组BDNF阳性细胞数,并进行图像分析.结果:CTP组及缺血组BDNF两组细胞形态无明显不同,但CTP治疗组阳性细胞数显著高于相应缺血对照组.结论:三磷酸胞苷可提高大鼠局灶脑缺血半暗带区BDNF的表达水平.     

三磷酸胞苷对脑缺血大鼠脑源性神经营养因子的影响

目的:观察CTP对局灶脑缺血大鼠BANF表达的影响。方法:应用免疫组化方法观察不同缺血时间CTP治疗组及脑缺血组BDNF阳性细胞数,并进行图像分析。结果:CTP组及缺血组BDNF两组细胞形态无明显不同,但CTP治疗组阳性细胞数显著高于相应缺血对照组。结论:三磷酸胞苷可提高大鼠局灶脑缺血半暗带区BDNF的表达水平。     

脑源性神经营养因子与卵巢

脑源性神经营养因子(BDNF)是广泛分布于神经系统的一种生长因子,近年研究发现BDNF及其受体在卵巢中也有表达。 BDNF参与颗粒细胞功能的完成和卵母细胞体外发育的调控。对于BDNF及其受体TrkB在人卵巢的表达、生物学作用、信号转导机制还有待进一步研究。     

脑源性神经营养因子与卵巢

脑源性神经营养因子(BDNF)是广泛分布于神经系统的一种生长因子,近年研究发现BDNF及其受体在卵巢中也有表达.BDNF参与颗粒细胞功能的完成和卵母细胞体外发育的调控.对于BDNF及其受体TrkB在人卵巢的表达、生物学作用、信号转导机制还有待进一步研究.     

脑源性神经营养因子与卵巢

脑源性神经营养因子(BDNF)是广泛分布于神经系统的一种生长因子，近年研究发现BDNF及其受体在卵巢中也有表达。BDNF参与颗粒细胞功能的完成和卵母细胞体外发育的调控。对于BDNF及其受体TrkB在人卵巢的表达、生物学作用、信号转导机制还有待进一步研究。     

Decreased plasma brain-derived neurotrophic factor levels in institutionalized elderly with depressive disorder

ObjectivesTo compare the differences in plasma brain-derived neurotrophic factor (BDNF) levels among institutionalized ethnic Chinese elderly participants with major depression, those with subclinical depression, and a nondepressed control group.DesignA cross-sectional study.SettingThe veterans' home in southern Taiwan.ParticipantsOne hundred sixty-seven residents.MeasurementsQuestionnaires including the Minimum Data Set Nursing Home 2.1, Chinese-language version, and the short-form Geriatric Depression Scale, Chinese-language version. Depressive disorder was diagnosed by a well-trained psychiatrist using DSM-IV-TR (Diagnostic and Statistical Manual of Mental Disorders, 4th edition, text revision) criteria. We measured plasma BDNF levels in the following 3 groups: nondepressive subjects (n = 122), subclinically depressive subjects (n = 33), and subjects with major depression (n = 12). Plasma BDNF was assayed using the sandwich ELISA method.ResultsWe noted a significantly negative association between age and plasma BDNF in the regression model. There was no significant correlation between BDNF plasma levels and body weight or platelet counts. We found that plasma BDNF was significantly lower in the major depressive group (mean, 115.1 pg/mL; SD, 57.2) than in the nondepressive group (mean, 548.8 pg/mL; SD, 370.6; P < .001). The BDNF plasma concentrations in the subclinically depressive group (mean, 231.8 pg/mL; SD, 92.4; P < .001) and control group were also significantly different.ConclusionsOur findings revealed that plasma BDNF levels were reduced not only in ethnic Chinese elderly patients with major depressive disorder but also in those with subclinical depression. This makes the plasma BDNF level a potential biological marker for clinical or subclinical depression.     

Alterations of serum brain-derived neurotrophic factor levels in early alcohol withdrawal

AIMS: Alcohol withdrawal-enhanced neurotoxicity contributes to the addictive process. Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal plasticity and learning. In this study, we explored the changes of serum BDNF levels in alcoholic patients at baseline and after one-week alcohol withdrawal. METHODS: Twenty-five alcoholic patients were admitted for alcohol detoxification treatment, and 22 healthy control subjects were also enrolled. We collected blood samples of the patient group on the first and seventh day of alcohol withdrawal, and measured serum BDNF level with sandwich enzyme-linked immunosorbent assay. The severity of withdrawal symptoms was evaluated by the Clinical Institute Withdrawal Assessment-Alcohol, Revised every eight hours. RESULTS: Serum BDNF levels did not differ significantly between alcoholic patients and control subjects. But BDNF levels were found to be significantly increased one week after alcohol withdrawal (from 13.9 +/- 3.8 ng/ml to 15.4 +/- 3.8 ng/ml, P = 0.03). A significant positive correlation was found between baseline BDNF level and baseline withdrawal severity (r = 0.45, P = 0.03). CONCLUSIONS: The present study suggests that elevated serum BDNF levels were found in early alcohol withdrawal, implying that BDNF may involve in neuroadaptation during the period.     

脑源性神经营养因子预处理对大鼠脑缺血再灌注损伤氧化应激及神经细胞凋亡的影响

目的探讨不同剂量的脑源性神经营养因子（BDNF）预处理对大鼠局灶性脑缺血再灌注（I／R）损伤氧化应激及神经细胞凋亡的影响。方法采用大鼠大脑中动脉线栓法（MCAO）建立局灶性脑I／R损伤模型,BDNF于缺血前12h经侧脑室注射给药。采用黄嘌呤氧化酶法和硫代巴比妥酸法检测脑组织超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量,TUNEL法检测脑皮层凋亡神经细胞,免疫组化法检测Bcl-2、Bax蛋白表达。结果与I／R组比较,BDNF预处理各组脑组织SOD活性明显增强,MDA含量明显降低,给药各组中脑皮层Bcl-2蛋白表达明显增高,而Bax蛋白表达及TUNEL阳性细胞指数均明显降低（P〈0．01）;在BD-NF预处理各组中,以BDNF预处理高剂量组脑组织SOD活性及Bcl-2蛋白表达最高、MDA含量和Bax蛋白的表达以及TUNEL阳性细胞指数最低（P〈0．01）。结论不同剂量的BDNF预处理均能明显减轻脑I／R后氧化应激和神经细胞凋亡,具有不同程度的脑保护作用,其保护作用与BDNF预处理的剂量有关。其机制可能与BDNF预处理能够提高机体内源性抗氧化物质的含量及调节凋亡相关基因的不同表达有关。     

脑源性神经营养因子在PCOS患者卵泡发育中的作用

目的:探讨脑源性神经营养因子(BDNF)在卵泡发育和卵母细胞成熟过程中的作用。方法:采用ELISA法检测PCOS卵泡液中BDNF含量。结果:所有61例标本均检测出BDNF。IVM-PCOS组BDNF最低205 pg/ml、最高804 pg/ml,平均512 pg/ml;IVF-PCOS组BDNF最低540 pg/ml、最高1 303 pg/ml,平均888 pg/ml;IVF-Normal组BDNF最低368 pg/ml、最高1 089 pg/ml,平均734 pg/ml。三组间比较或两两比较差异均有统计学意义(P<0.001)。BDNF水平以IVF-PCOS组最高、IVF-Normal组次之、IVM-PCOS组最低。结论:成熟卵泡颗粒细胞存在BDNF的表达,BDNF可能与卵泡成熟及PCOS的发生发展有关。     

Effects of olive polyphenols administration on nerve growth factor and brain-derived neurotrophic factor in the mouse brain

ObjectivePolyphenols are chemicals derived from plants known to possess antioxidant and anti-inflammatory properties. High intake of fruit and vegetables is believed to be beneficial to human health. Various studies have suggested that dietary polyphenols may protect against cancer and cardiometabolic and neurodegenerative diseases. Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neurotrophins that play key roles in brain cell development, growth, and survival. The aim of this study was to investigate whether or not administration of olive (Olea europaea L.) polyphenols could have an effect on NGF and BDNF content and the expression of their receptors, TrkA and TrkB, respectively, in the mouse brain.MethodsNGF and BDNF were measured by enzyme-linked immunosorbent assay. TrkA and TrkB were measured by Western blotting.ResultsWe found NGF and BDNF elevation in the hippocampus and olfactory bulbs and a decrease in the frontal cortex and striatum. These data were associated with potentiated expression of TrkA and TrkB in the hippocampus and olfactory bulbs but no differences between groups in the striatum and frontal cortex. Polyphenols did not affect some behavioral mouse parameters associated with stressing situations.ConclusionsAltogether, this study shows that olive polyphenols in the mouse may increase the levels of NGF and BDNF in crucial areas of the limbic system and olfactory bulbs, which play a key role in learning and memory processes and in the proliferation and migration of endogenous progenitor cells present in the rodent brain.     

Relation between plasma brain-derived neurotrophic factor and nerve growth factor in the male patients with alcohol dependence

Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) are thought to be related to neuroprotection in cell culture and animal studies. Our aim was to verify the changes in human plasma BDNF and NGF concentrations induced by chronic alcohol use. Forty-one male patients with alcohol dependence were sampled the next morning of admission and compared with 41 healthy male subjects. Plasma BDNF and NGF were assayed using an enzyme-linked immunosorbent assay (ELISA). Mean plasma BDNF level was significantly higher in the patients with alcohol dependence (3502.21 ± 1726.9 pg/mL) compared with the healthy subjects (861.75 ± 478.9 pg/mL) (P = .000). Mean plasma NGF level was also significantly higher in patients with alcohol dependence (137.64 ± 32.7 pg/mL) than in healthy subjects (112.61 ± 90.2 pg/mL) (P = .012). Plasma BDNF and NGF levels showed significant negative correlation in alcohol dependence group (r = −0.388, P = .012). Increased plasma BDNF and NGF with negative correlation in alcohol-dependent patients may have some role in the regeneration of damage done by chronic alcohol use.     

血液脑源性神经营养因子水平与阿尔茨海默病关系的Meta分析

目的探讨阿尔茨海默病(Alzheimer disease,AD)患者血液脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)水平变化情况。方法检索中国知网、万方、维普数据库、中国生物医学文献数据库、Pub Med、西文生物数据库有关AD患者血液BDNF变化的病例对照研究。检索年限均为建库以来至2016年5月。采用纽卡斯尔渥太华量表(Newcastle Ottawa scale,NOS)标准文献质量进行评价。用Stata12.0软件进行Meta分析,检验水准为α=0.05。结果总计11篇文献纳入本次研究,其中英文文献7篇,中文文献4篇。NOS评分7~8分。病例组血液BDNF水平低于对照组,两组比较差异有统计学意义(Z=2.331,P=0.020)。年龄、地区为可能异质性因素。以病例组中位年龄73.2岁为分界线,结果表明年龄较大组,AD患者血液BDNF水平与正常对照组比较差异有统计学意义(P0.05);年龄较小组与正常对照组比较差异无统计学意义(P0.05)。以是否为亚洲人群进行分析,结果表明尚不能认为亚洲人群病例组血液BDNF水平与正常对照组比较差异有统计学意义(P0.05),而非亚洲人群两组BDNF水平比较差异有统计学意义(P0.05)。固定效应模型和随机效应模型计算结果趋向一致,提示合并结果可靠。用Begg’s test检验漏斗图对称性研究没有明显的发表偏倚存在。结论血液BDNF水平下降可能与AD的发生有关。     

血液脑源性神经营养因子水平与强迫症关系的Meta分析

目的综合分析血液脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)水平与强迫症(obsessivecompulsive disorder,OCD)的关系。方法在万方、中国知网及Pub Med中检索,入选关于血液BDNF水平与OCD关系的病例对照研究,采用Stata12.0软件对数据进行Meta分析,P0.05为差异有统计学意义。结果共有7篇文献纳入分析,获得样本543例,病例组308例,对照组235例。Meta分析结果显示,OCD患者血液BDNF水平低于对照组(合并效应值SMD=-1.466,95%CI为-2.346~-0.585,P0.05)。敏感性分析显示,两组在血液BDNF水平上的差异结果可靠。Bgger检验显示无明显的发表偏倚。结论血液BDNF水平下降可能与OCD的发生有关。     

Effects of zinc supplementation on efficacy of antidepressant therapy,inflammatory cytokines,and brain-derived neurotrophic factor in patients with major depression

Objective

Zinc is found in abundance in the human brain. Patients with depression may have decreased consumption of food sources rich in zinc, and zinc supplementation may have a potential influence on depressive symptoms. However, clinical trials on the effect of zinc supplementation in depression are limited. The objective of the present study was to determine the effect of zinc supplementation on efficacy of antidepressant therapy. Furthermore, the effect of zinc on plasma levels of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and brain-derived neurotrophic factor-a (BDNF-a) were assessed.

Design

A single-center, randomized, double-blind, placebo-controlled trial of zinc supplementation was conducted in patients with DSM-IV major depression. Forty-four patients of both sexes aged 18–55 years were recruited for this study from a university hospital. The zinc-supplemented group received zinc sulfate (25 mg elemental Zn/day) orally in addition to their selective serotonin reuptake inhibitor antidepressants for 12 weeks. Symptoms were evaluated using the Hamilton Depression Rating Scale (HDRS) on arrival, weeks 6 and 12. Plasma levels of IL-6, TNF-α and BDNF-a were measured at baseline and at the end of study.

Results

Twenty patients in zinc group and 17 patients in placebo groups completed the study. At baseline, there were no significant differences in any variable between the patients allocated to receive placebo and those taking zinc supplement. Zinc supplementation significantly reduced HDRS compared to placebo (P < 0.01 at 12th week). No significant differences were observed in plasma levels of IL-6, TNF-α, and BDNF-a between zinc-supplemented and placebo-supplemented group.

Conclusion

Zinc supplementation in conjunction with antidepressant drugs might be beneficial for reducing depressive symptoms. However, its effect does not appear to be mediated through impact of zinc on inflammatory processes.