改良桃红四物汤对使用止血带后肢体缺血再灌注损伤保护作用的临床研究

〔摘 要〕 目的：筛选神经母细胞瘤（NB）中 MYCN 相关的长链非编码核糖核酸（lncRNA）,分析其功能及对患者预后的影响,寻找潜在治疗靶点和分子标志物。 方法：分别从 TARGET 及 GEO 数据库下载基因表达数据,并将两组数据分成 MYCN 高表达组和低表达组,R 语言筛选出两组差异表达 lncRNA,取共同差异表达 lncRNA;通过 ENCORI 数据库预测与 lncRNA 互作的核糖核酸（RNA）,DAVID 数据库分析相关基因的功能与信号通路的富集情况;TARGET 数据库进行相关生存分析。 结果：TARGET 数据库中共筛选出差异倍 数在两倍以上的 lncRNA 1104 个,GEO 数据库筛选出差异基因2849 个,两个数据库中共同差异表达 lncRNA 59 个,ENCORI 数据库预测与这些基因互作 mRNA 547 个,功能分析发现这些基因主要富集在调控神经系统疾病的相关通路,并通过调控转录、蛋白绑定等发挥相关生物学功能。利用 TARGET 数据库进行生存分析发现其中 VPS9D1–AS1、LINC00649、LINC01234 的异常表达可以影响患者生存。 结论：运用生物信息学通过对 MYCN 相关差异表达 lncRNA 的分析发现 VPS9D1–AS1、LINC00649、LINC01234 可能与 NB 的发生发展密切相关,可为后续的研究提供方向与思路。     

加味桃红四物汤治疗中风后抑郁36例

中风后抑郁是脑血管病常见并发症之一，在中风病患者中，有较高的发生率，是影响患者功能恢复和中风复发的独立危险因素，笔者试用中药治疗，取得了较好疗效，现报道如下。     

桃红四物汤新用

桃红四物汤是补血活血化瘀之良方,笔者在临床将此方广泛运用于多种疾病,疗效满意,现谨举几例,供同道参考。 1 鹅掌风:高某,男,41岁,农民,1989年5月16日初诊。患者3年前右手掌被刺刺肿,嗣后即起水疱,燥痒难忍,水疱破后叠起白皮,皮肤肌肉渐渐粗糙变厚,结痂皲裂疼痛,手不能握固,曾服中西药,均无效。其证乃毒邪蕴结,迁延日久,必有瘀血,非活血化瘀不足以解其顽疾。乃处:桃仁、红花、大黄各6g,当归、赤芍、白芍、生地各10g,蒲公英30g,薏苡仁30g,丹参15g。连续服38剂,结痂消失,皮肤肌肉恢复正常而痊愈,至今未复发。按:鹅掌风乃皮肤破损,邪毒感染,风热毒邪蕴结凝聚于皮肤,气血不通,肌肤失     

桃红四物汤的研究概况

桃红四物汤出血《医宗金鉴》，为调经之方。近年来广泛用治临床各科疾病，疗效满意。实验研究亦为其提供了科学依据。本文综述了１０年来有关本方的研究概况。     

桃红四物汤新用

1　顽固性偏头痛杨××,女,35岁,1995年8月26日初诊。右侧偏头痛反复发作近8年。近年来每于工作劳倦时发作,多为刺痛,伴经行腹痛及习惯性便秘。西医诊断为血管神经性头痛。诊见舌黯红、边有瘀斑,苔薄微黄,脉弦细而涩。治宜活血化瘀,祛风止痛。拟桃红四物汤加味。处方:桃仁9ｇ,红花、川芎各6ｇ,当归10ｇ,生地、赤芍、草决明、钩藤(后下)各15ｇ,全蝎5ｇ,蜈蚣(去头足)2条。3剂,水煎服。药后头痛大减,大便通畅,睡眠改善,精神转佳。守原方连服7剂,头痛全止。随访3个月未见复发,且经行腹痛症亦随之消失。按:本例顽固性偏头痛,病程达8年之久,反复发…     

加味桃红四物汤治疗缺血性脑卒中后抑郁症67例疗效观察

缺血性脑卒中后抑郁症(PSD)的治疗一直是医学上的热点话题,本文从临床上运用加味桃红四物汤来治疗该病,把病人分为两组,一直使用加味桃红四物汤来治疗,一组不加,最后得出药物对病情的治疗作用.     

桃红四物汤新解

桃红四物汤古代主要是应用于妇科月经病,现代各家对其有了更广泛的理解,使其应用于全身各个系统病证。     

针刺治疗缺血性脑卒中的机制研究进展

通过查阅近年来关于针刺治疗缺血性脑卒中(CIS)的相关文献,发现针刺治疗CIS与抑制神经炎性反应、减轻氧化应激、抑制兴奋性氨基酸毒性、抵抗神经元凋亡、调节自噬、促进神经元再生和修复、促进血管重塑、调节脑血管储备、调节脑代谢、维持血脑屏障完整性等多种作用机制密切相关.本文对以上机制进行归纳总结,以期为临床应用针刺治疗CI...     

Immunosuppressive effects of Echinodorus macrophyllus aqueous extract

Echinodorus macrophyllus is a medicinal plant, popularly known in Brazil as "chapéu de couro", used to treat rheumatic diseases, which are usually characterized by exacerbated T and B lymphocyte response. We have evaluated the effects of the aqueous extract of Echinodorus macrophyllus (AEEm) on these cell functions, proliferation, and nitric oxide production. Mice treated orally for 7 days with AEEm had inhibited B cell antibody production (0.5mg/kg b.w.) and delayed type hypersensitivity (0.5 and 5mg/kg b.w.) mediated by T cells, reducing subcutaneous tissue leukocyte infiltration. AEEm inhibited, in vitro, NO production by stimulating J774 cells in a dose-dependent manner, with no cytotoxicity. We have demonstrated, for the first time, its immunosuppressive effect. This immunosuppressive effect supports a potential therapeutic use of AEEm to control exacerbated humoral and/or cellular immune response, as in autoimmune rheumatic diseases. However, it is important to be cautious about its indiscriminate popular use to avoid side effects, mainly in immunodeficiency diseases.     

Anxiolytic effects of the aqueous extract of Uncaria rhynchophylla

The purpose of this study was to characterize the putative anxiolytic-like effects of the aqueous extract of hooks with stem of Uncaria rhynchophylla using the elevated plus maze (EPM) and the hole-board apparatus in rats and mice. Control rats were treated with an equal volume of saline, and positive control rats with buspirone (1 mg/kg). Single or repeated treatments of the aqueous extract of Uncaria rhynchophylla (200 mg/kg/day, p.o.) for 7 days significantly increased the time-spent and entries into open arms of the EPM, and reduced the time-spent and entries into the closed arms versus saline controls (P<0.05). However, no changes in spontaneous locomotor activity or myorelaxant effects were observed versus saline controls. In the hole-board test, repeated treatment with the aqueous extract of Uncaria rhynchophylla (100 or 200 mg/kg/day, p.o.) significantly increased the number of head-dips (P<0.05). In addition, the anxiolytic-like effects of Uncaria rhynchophylla extract as assessed using the EPM test were abolished by WAY 100635 (0.3 mg/kg, i.p.), a 5-HT(1A) receptor antagonist. These results suggest that Uncaria rhynchophylla is an effective anxiolytic agent, and acts via the serotonergic nervous system.     

Cardiovascular effects of the aqueous extract of Moringa pterygosperma

The aqueous extract of stem bark of Moriga pterygosperma (Family Moraingaceae) was investigated for its effect on various pharmacological parameters. In cardiovascular profile at lower concentrations (1–10 ng) it produced a dose dependent positive inotropic effect (n = 3, 1.29 ± 0.021 for 10 ng) and at higher concentrations (0.1–1 μg) a dose dependent negative inotropic effect (n = 3, 0.53 ± 0.033 for 1 μg) on the isolated frog heart. It also produced a dose dependent hypotensive effect on dog blood pressure (n = 3, 82 ± 0.98 for 20 mg/kg). It failed to elicit any effect on isolated guinea-pig ileum, rat stomach fundus or frog rectus abdominis muscle.     

黄连解毒汤对脑缺血小鼠的抗氧化作用研究

目的 :研究黄连解毒汤提取物对脑缺血小鼠的抗氧化作用。方法 :测定了脑缺血小鼠大脑皮质及海马组织中丙二醛(MDA)、谷胱甘肽 (GSH)的含量及超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)、过氧化氢酶 (CAT)的活力。结果 :黄连解毒汤提取物 2 0 g/kg、4 0g/kg或 8 0 g/kg能显著降低脑缺血小鼠大脑皮质及海马组织中MDA的含量 ,显著增加脑缺血小鼠脑中GSH的含量 ;能显著提高脑缺血小鼠大脑皮质及海马组织中SOD、GSH Px和CAT的活力。结论 :黄连解毒汤提取物对脑缺血小鼠脑组织有很强的抗氧化作用。     

Anti-metastatic effects of aqueous extract of Helixanthera parasitica

Metastasis, the spread of cancer in body, is a major cause of death. We have screened anti-metastatic activity of aqueous and dichloromethane extracts of several not previously studied Thai herbs, using an in vitro invasion test. This involves the in vitro invasion of HCC-S102, a hepatocellular carcinoma cell line derived from a Thai patient, through a reconstituted-basement membrane (Matrigel). The aqueous extract of a plant (Helixanthera parasitica) revealed a significant inhibitory effect on the cancer cell invasion, and showed antioxidant activity. The aqueous extract was partially purified by silica gel column chromatography, and the highest anti-metastatic activity fraction showed 83% inhibition of invasion with low cytotoxic effect. However, anti-metastatic activity was not associated with the antioxidant activity of the aqueous extract.     

缺血性中风病机探析

中风病的致病原因,历代多有争议,现在"风、火、痰、瘀、气、虚"等病因被广大学者认同,但多强调气血逆乱。对于基本病机,观点不一,试参照历代医家对本病的病机认识,结合现代医学,探求缺血性中风病病因病机。     

The antianaphylactic effects of Crinum glaucum aqueous extract

The aqueous extract of Crinum glaucum was evaluated for its effects on antigen and histamine induced contraction of the ileum and on mediator release from the lungs of sensitized guinea pigs. The results show that the extract dose dependently inhibited the contractions induced by antigen and histamine in vitro and in vivo. The extract also inhibited, in a dose-dependent manner, the quantity of mediators antigenically released from the lungs and reduced the mepyramine resistant activity from the lungs. The results obtained indicate that the extract possesses antianaphylactic properties, which may account for its use as an antiasthmatic in traditional medicine.     

丹参酮类脂溶性成分及其干预缺血性脑卒中机制的研究进展

缺血性脑卒中是一种由于脑部供血受阻导致缺血灶不可逆损伤的中枢神经系统疾病,其发病突然、传变迅速、病程复杂,静脉溶栓和血管内介入是早期干预的主要措施,但时间窗和禁忌症使其应用受到限制.丹参Salviae Miltiorrhizae Radix et Rhizoma为传统中药材,其中丹参酮类脂溶性成分治疗缺血性脑卒中疗效确...     

缺血性中风中医证候演变与中风复发关系研究

目的探讨包括中医证候演变特点在内的多种复发因素对于缺血性中风的影响。方法前瞻性收集339例急性期缺血性中风病患者资料,随访1年,运用Cox回归分析多种可能因素对于缺血性中风复发的影响。结果缺血性中风患者1年复发率为11.7%(39/333),大动脉粥样硬化性脑梗死复发风险是小动脉闭塞性脑梗死的2.573倍,恢复期阴虚阳亢证显现的患者复发风险是未显现患者的3.991倍,男性复发风险是女性的2.74倍,急性期使用中药汤剂患者复发风险是未使用患者的34.3%。结论大动脉粥样硬化性脑梗死、男性患者、缺血性中风恢复期阴虚阳亢证的显现是中风复发的独立危险因素,急性期中药汤药治疗是复发的保护性因素。