Effect of Ligustrazine on Nitric Oxide Contents in Cerebrospinal Fluid and Plasma of Patients with Cerebral Infarction

Effect of Ligustrazine on Nitric Oxide Contents in Cerebrospinal Fluid and Plasma of Patients with Cerebral Infarction@李德洋@石义亭@陈玉萍@李茹@戴锋     

Clinical Study on Effect of Shenfu Injection(参附注射液)in Treating Senile Cerebral Infarction

Objective:To explore the clinical effectand mechanism of Shenfu Injection(SFI)intreating senile cerebral infarction.Methods:Eighty-two aged patients with cerebral in-farction were randomly divided into the trea-ted group treated with SFI and the controlgroup treated with Venoruton.The clinicaleffect was observed,the hemorrheologic and     

Effect of Tongxinluo Capsule (通心络胶囊) on Plasma Endothelin,Calcitonin Gene-related Peptide and Nitrogen Oxide of Acute Cerebral Infarction Patients

Research of TCM shows that the chief pathological basis of acute cerebral infarction (ACI) is "blood stasis obstructing collateral". Here the authors used Tongxinluo capsule (通心络胶囊, TXLC) for replenishing qi, removing stasis and dredging collateral to treat qi deficiency-blood stasis type of ACI, and observed the change of plasma endothelin (ET), calcitonin gene-related peptide (CGRP) and nitrogen oxide (NO), aiming at exploring the effect of TXLC on vascular endothelial function and neurotransmitter.     

Clinical Observation on 30 Cases of Transient Cerebral Ischemia Attack Treated with Acupuncture and Medication

Objective: To probe the curative effect of acupuncture and medication on transient cerebral ischemia attack. Method: 30 patients with transient cerebral ischemia attack in the treatment group were acupunctured at Fengchi (GB 20), Wangu (GB 12) and Tianzhu (BL 10) and given orally leech capsules and centipede capsules. 30 patients with transient cerebral ischemia attack in the control group were given intravenous drip of compound Danshen injection and orally aspirin. At the end of two treatment courses, the curative effects were evaluated and the changes in blood rheology and in 3 indexes of blood coagulation were observed before and after treatment in the 2 groups. Results: The total effective rate in the treatment group was 86.7% with obvious difference as compared to the control group (P<0.05). There were remarkable differences in blood rheology and 3 indexes of blood coagulation before and after treatment in the treatment group (P<0.05, P<0.01). There were remarkable differences after treatment between the 2 groups (P<0.05). Conclusion: Acupuncture at Fengchi (GB 20), Wangu (GB 12) and Tianzhu (BL 10) and medication with leech capsules and centipede capsules are effective methods in treating transient cerebral ischemia attack.     

A Clinical Study of Plasma Fibronectin in Patients with Severe Viral Hepatitis

Plasma fibronectin was determined serially with both simpleradioimmunadiffasion and modified indirect hemoagglutination test(usinggelatin-coated sheep RBC)in 30 patients with severe viral hepatitis(SVH),100normal subjects and 30 cases of chronic active hepatitis(CAH)were also studiedto serve as controls.Liver function test,serum complement 3 and immunoglobulinwere determined simultaneously.Endotoxin was assayed with limulus lysate testin 21 cases of SVH and 14 cases of CAH.The normal value of plasma fibronectin was 284.31±45.56μg/ml and 1:30.64(geometrical mean).Plasma fibronectin level decreased significantly in SVHpatients.Obvious correlations were found between the levels of plasma fibronectinand the valuers of total serum bilirubin,prothrombin time,complement 3,immunoglobulin M,and blood platelet count.Low plasma fibronectin levels wereremarkably associated with the occurrence of endotoxemia.It is concluded that serial determination of plasma fibronectin can reflect theseverity of the illness and the prognosis of the patients with SVH.     

Effects of Electroacupuncture plus Intra－carotid Drug Injection on Rheoencephalogram in Patients with Cerebral Infarction

Purpose: To investigate the mechanism of electroacupuncture (EA) plus intra-carotid drug injection for treating cerebral infarction. Methods: Rheoencephalogram was recorded with a RG-2B type of bridge rheoencephalograph and findings were compared before and after the treatment. Results: After the treatment, the prolonged rising time was shortened, and the decreased amplitude obviously elevated.Conclusion: The therapy can dilate cerebral blood vessels, increase the cerebral blood flow, and improve the elasticity of cerebral blood vessels, leading to sufficient blood and oxygen supply in the ischemic brain tissues and to restoration of their functions.     

A Correlative Study on Serum Lp(a) in Patients of Cerebral Infarction with thatin Their Childre

Serumlipoprotein(a){Lp(a)}levelisgeneticallydeterminedandremainsalmostconstantthroughoutlife.Itselevationhassignificantfamiliarcentripetaltendency.Basedonitsproperties,weselectedthepatientswithcerebralinfarction(CI)anddefinitefamilyhistoryofthediseas...     

Clinical Study on Influence of Buyang Huanwu Decoction (补阳还五汤) on the Metabolic Imbalance of Endothelin and Calcitonin Gene Related Peptide in Patients with Early Cerebral Infarction

ＦｒｏｍＭａｙ1995ｔｏＭａｙ1997,ＢｕｙａｎｇＨｕａｎｗｕＤｅｃｏｃｔｉｏｎ（补阳还五汤,ＢＨＤ）ｗａｓｕｓｅｄｂｙｔｈｅａｕｔｈｏｒｓｉｎｔｒｅａｔｉｎｇｐａｔｉｅｎｔｓｗｉｔｈｃｅｒｅｂｒａｌｉｎｆａｒｃｔｉｏｎｔｏｅｘｐｌｏｒｅｉｔｓｅｆｆｅｃｔｏｎｍｅｔａｂｏｌｉｃｄｉｓｔｕｒｂａｎｃｅｏｆｅｎｄｏｔｈｅｌｉｎ（ＥＴ）ａｎｄｃａｌｃｉｔｏｎｉｎｇｅｎｅｒｅｌａｔｅｄｐｅｐｔｉｄｅ（ＣＧＲＰ）．Ａｎｏｔｈｅｒｇｒｏｕｐｏｆｐａｔｉｅｎｔｓｗｅｒｅｔｒｅａｔｅｄｗｉｔｈｗｅｓｔｅｒｎｍｅｄｉ…     

The Effect of Acupuncture on Serum IgE Level in Patients with Chronic Urticaria

The pathogenesis for chronic urticaria is not clear yet, and there has been no assured effective therapies for it. In the present study on the therapeutic effects of acupuncture for chronic urticaria and its mechanisms in immunity, the author put the stre…     

Effect of Acupuncture on Plasmic Levels of Insulin,Glucagon and Hypercoagulability in NIDDM Complicated by Acute Cerebral Infarction

Twenty-one cases of acute cerebral infarction secondary to NIDDM were treated with acupunctureand conventional therapy,and compared with 16 cases treated with conventional therapy alone.Theresults showed that acupuncture was more effective in reducing insulin and glucagon levels(P<0.001)and improving hypercoagulability(P<0.05) of blood.     

A Meta-analysis of β-fibrinogen Gene-455G/A Polymorphism and Plasma Fibrinogen Level in Chinese Cerebral Infarction Patients

Objective To evaluate the correlation between the β-fibrinogen gene-455G/A polymorphism and cerebral infarction in Chinese population by means of meta-analysis. Methods Genetic association studies on evaluating the β-fibrinogen gene -455G/A polymorphism and cerebral infarction involving Chinese population published before December 2005 were collected from database of PubMed, EMBASE, and CNKI. All the data in literature were abstracted based on the defined selection criteria by two independent investigators. Publication bias was tested by funnel plot and the odd ratios of all studies were combined dependent on the result of heterogeneity test among the individual studies. The software Review Manager (Version 4.2) was used for meta-analysis. Results Eleven studies including 1405 patients and 1600 controls met the selection criteria. There was no publication bias in 11 reviewed studies. Heterogeneity test of reviewed studies showed statistically significant differences (χ2=24.58, P=0.006) among the ORs of individual studies. The combined OR of 11 studies of susceptibility to cerebral infarction in –455A allele carriers compared with the -455G/G wild homozygotes was 1.33 (95%CI 1.04-1.71, P=0.02). In the patients with cerebral infarction in 6 studies, the summarized average plasma fibrinogen level of allele A carrier was 0.29 g/L (95%CI 0.14-0.44, P=0.0002) higher than that of -455G/G homozygous ones. Conclusions β-fibrinogen gene -455G/A polymorphism might contribute to susceptibility of cerebral infarction in Chinese population; allele A increases the individual susceptibility to the disease.     

Effect of Puerarin on Plasma Endothelin, Renin Activity and Angiotensin Ⅱ in Patients with Acute Myocardial infarction

Ｅｆｆｅｃｔ　ｏｆ　Ｐｕｅｒａｒｉｎ　ｏｎ　Ｐｌａｓｍａ　Ｅｎｄｏｔｈｅｌｉｎ，　Ｒｅｎｉｎ　Ａｃｔｉｖｉｔｙ　ａｎｄ　Ａｎｇｉｏｔｅｎｓｉｎ　Ⅱ　ｉｎ　Ｐａｔｉｅｎｔｓ　ｗｉｔｈ　Ａｃｕｔｅ　Ｍｙｏｃａｒｄｉａｌ　ｉｎｆａｒｃｔｉｏｎＬＩＳｈｕ－ｍ...     

Perioperative Changes of Plasma Endothelin—1 in Patients Undergoing Coronary Artery bypass Grafting and the Effect of Nitroglycerin

Ｅｎｄｏｔｈｅｌｉｎ (ＥＴ)ｉｓｔｈｅｍｏｓｔｐｏｔｅｎｔｅｎｄｏｇｅ ｎｏｕｓｖａｓｏｃｏｎｓｔｒｉｃｔｏｒｉｄｅｎｔｉｆｉｅｄｓｏｆａｒ.ＥＴ 1ｉｓｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｂｉｏｌｏｇｉｃａｌｌｙａｃｔｉｖｅｏｆｔｈｅｅｎｄｏｔｈｅ ｌｉｎｉｎｈｕｍａｎｂｅｉｎｇｓ,ｍａｙｂｅｉｎｖｏｌｖｅｄｉｎｔｈｅｐａｔｈｏｌｏｇｉｃｐｒｏｃｅｓｓｏｆａｔｈｅｒｏｓｃｌｅｒｏｓｉｓｉｎｃｏｒｏｎａｒｙａｒｔｅｒｙ[1] ,ａｎｄａｌｓｏｐｌａｙｓａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎａｎｔｉ ｎｉｔｒｉｃｏｘ…     

Clinical Efficacy of Traditional Chinese Medicine on Acute Myocardial Infarction——A Prospective Cohort Study

<正>Objective:To evaluate the clinical effects of Chinese medicine(CM) on acute myocardial infarction (AMI) with a prospective cohort study.Methods:A total of 334 AMI patients from January 2007 to March 2009 were consecutively enrolled,and were assigned to a treatment group(169 cases) treated with combined therapy(CM for at least one month and Western medicine) and a control group(165 cases) with Western medicine alone.Clinical data including age,gender,smoking,medical history,infarction area,heart functional classification,CM syndrome scores,blood-stasis syndrome score,primary end-point(death,nonfatal myocardial infarction,and revascularization) and secondary end-point(ischemic stroke,rehospitalization due to angina,heart failure and shock),were collected. CM syndrome scores,blood-stasis syndrome score,primary end-point and secondary end-point were collected during the 6-month follow-up.Kaplan-Meier method was used for the survival analysis.The multifactor analysis was analyzed by Cox proportional hazards regression.Results:At the end of 6-month the CM syndrome score and blood- stasis syndrome score in the treatment group were lower than those in the control group(P0.01),especially the symptoms of chest pain,spontaneous perspiration and insomnia.Rehospitalization rate due to angina during the 6-month follow-up in the treatment group(2.96%) was lower than that in the control group(7.88%,P0.05).Kaplan- Meier survival curve showed that event-free cumulated survival of rehospitalization due to angina during the 6-month follow-up in the treatment group was higher than that in the control group(Log rank 4.700,P=0.03).Cox regression analysis showed that heart dysfunction[hazard ratio(HR)=1.601,95%CI=1.084-2.364,P=0.018]and diabetes mellitus(HR=1.755,95%Cl=1.031-2.989,P=0.038) were hazard factors to end-point,whereas CM(HR 0.405,95% Cl=0.231-0.712,P=0.002),percutaneous coronary intervention(PCI,HR=0.352,95%CI=0.204-0.607,P0.001) and angiotensin converting enzyme(ACE) inhibitors(HR=0.541,95%Cl=0.313-0.936,P=0.028) were protective factors.Conclusions:CM therapy could decrease CM syndrome scores and blood-stasis syndrome score,reduce the rehospitalization rate during 6-month follow-up due to angina.Heart dysfunction and diabetes mellitus were hazard factors to end-point,whereas CM,PCI and ACE inhibitors were protective factors.     

Study on Levels of Plasma Endothelin and Nitrii Oxide in Patients with Unstable Angina and lnfluence of Chinese Herbal Medicine

ＳｔｕｄｙｏｎＬｅｖｅｌｓｏｆＰｌａｓｍａＥｎｄｏｔｈｅｌｉｎａｎｄＮｉｔｒｉｉＯｘｉｄｅｉｎＰａｔｉｅｎｔｓｗｉｔｈＵｎｓｔａｂｌｅＡｎｇｉｎａａｎｄｌｎｆｌｕｅｎｃｅｏｆＣｈｉｎｅｓｅＨｅｒｂａｌＭｅｄｉｃｉｎｅ￥ＬＩＵＨｏｎｇ－ｘｕ；ＤＥＮＧＸ...     

The Expression of Endothelin Receptor B in Melanoma Cells A375 and Sk-mel-1 and the Proliferative Effects of Endothelin 3 on A375 Cells

In order to investigate the expression of endothelin receptor B （ETR-B） in human malignant melanoma （MM） cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 （ET3） on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.     

Clinical Observation on Therapeutic Effects of the Point-Penetrating Method in Acupuncture Treatment of Spastic Hemiparalysis Due to Cerebrovascular Disorders

Objective: To observe the therapeutic effects of different acupuncture methods for spastic hemiparalysis due to cerebrovascular disorders. Methods: 90 cases of spastic hemiparalysis after wind-stroke were randomly divided into a control group of 30 cases, a body acupuncture group of 30 cases and a point-penetrating acupuncture group of 30 cases. All the patients were given 4 courses of treatment, with 7 sessions constituting one therapeutic course and with a one-day interval between courses. The evaluations were made 3 times, i.e. once before treatment and once every 15 days during the treatment. Results: The therapeutic effects in the point-penetrating acupuncture group were obviously superior to those of both the control group and the body acupuncture group. Conclusion: The point-penetrating acupuncture is an effective therapy for treating spastic hemiparalysis due to cerebrovascular disorders.     

Effect of Xuesaitong Soft Capsule(血塞通软胶囊)on Hemorrheology and in Auxiliarily Treating Patients with Acute Cerebral Infarction

Objective: To observe the therapeutic effect of Xuesaitong soft capsule(血塞通软胶囊,XST)and its effect on platelet counts, coagulation factor 1 (CF1) as well as hemorrheologic indexes in treating patients with acute cerebral infarction (ACI). Methods: Two hundred and four patients with ACI were assigned into two groups, the control group ( n = 96) and the treated group ( n = 108). They were all treated with conventional Western medicines, including mannitol, troxerutin, citicoline, piracetam and aspirin, while to the treated group, XST was given additionally through oral intake, twice a day, 2 capsules each time for 8successive weeks. The clinical efficacy was evaluated according to the nerve function deficits scoring and the changes of platelet count. CF1 and hemorrhe.ological indexes were measured before and after treatment. Results: The total effective rate was 87.0% in the treated group, and 87.5% in the control group, respectively, showing insignificant difference between them. But the markedly effective rate in the treated group (66.7%) was significantly higher than that in the control group (27.1%, P＜0.01). The count of platelet was not changed significantly in both groups after treatment, while CF1 inthem evidently lowered at the end of the 4th and 8th weeks of treatment, but showed insignificant difference between the two groups. The hematocrit, whole blood viscosity and plasma viscosity in both groups were all improved significantly after treatment, but also showed insignificant difference in comparison of the two groups. Conclusion: XST has good efficacy in auxiliary treatment of patients with ACI, though its mechanism remains to be further explored.