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目的探讨谷胱甘肽过氧化物酶1(GPX-1)基因多态性与中国汉族儿童川崎病(kawasaki disease,KD)并冠状动脉损害(CAL)的相关性。方法采用聚合酶链反应-限制性内切酶片段长度多态性分析(PCR-RFLP)检测92例川崎病患儿及108例健康儿童GPX-1基因的-46C/T位点和599C/T位点多态性。结果川崎病组GPX-1基因-46C/T位点CC、CT、TT基因型分布和C、T等位基因频率与正常对照组比较差异无显著性意义(χ2=0.174和0.166,P均0.05)。川崎病组GPX-1基因599C/T位点CC、CT、TT基因型分布和C、T等位基因频率与正常对照组比较差异亦无显著性意义(χ2=0.429和0.368,P均0.05)。川崎病患儿合并CAL组与无冠状动脉损害(NCAL)组GPX-1基因-46C/T位点基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.507和0.487,P均0.05)。川崎病患儿合并CAL组与NCAL组GPX-1基因599C/T位点基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.635和0.535,P均0.05)。结论尚未发现GPX-1基因-46C/T位点多态性以及599C/T位点多态性与川崎病及其CAL的发生存在明显关联性。 相似文献
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目的探讨乙醛脱氢酶2(ALDH2)基因rs671位点与我国人群心血管代谢危险因素的关系。方法利用2000~2001年开展的中国心血管健康多中心合作研究(InterASIA),纳入北方5个省市共计6404例基线未患冠心病和脑卒中个体。分析rs671位点与11种心血管代谢危险因素(腰臀围、血脂和血压等)的关系。结果rs671 A等位基因频率为0.16。rs671与饮酒行为显著相关,携带A等位基因的个体不倾向于饮酒(P<0.001)。rs671与腰围、臀围、甘油三酯和舒张压显著相关(P<0.05),每增加一个A等位基因,分别减少0.61 cm、0.54 cm、4.39 mg/dl和0.68 mmHg。进一步校正饮酒行为后,rs671与臀围的关联仍然显著(P=0.035),但与舒张压的关联不再显著。在饮酒人群中rs671 A等位基因可降低舒张压1.28 mmHg,而在非饮酒人群中没有作用。结论本研究发现rs671位点与中国人群腰围和舒张压显著相关,其中与舒张压的关联主要是通过饮酒发挥作用的。 相似文献
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目的 研究中国人群TNFRSF1B基因多态性与冠心病的关系.方法 从山西医科大学第二临床医院选取208例冠心病患者和104例相匹配的对照者,并记录所有研究对象的病史、体格检查等临床资料及其它流行病学资料,采用聚合酶链反应和连接酶检测反应检测各组TNFRSF1B基因6号外显子rs1061624位点的基因型,并统计各组的多态性频率.结果 rs1061624的AA和GG等位基因和基因型频率在冠心病组和对照组的分布无统计学差异(冠心病组GG为33.5%,GA为52.8%,AA为13.7%;对照组GG为33.3%,GA为48.5%,AA为18.2%;P=0.52).在校正了年龄、性别、高血压病史、糖尿病史、甘油三酯和胆固醇等传统的危险因素后,以显性遗传模式分析发现G等位基因频率在冠心病组与对照组之间差异无统计学意义,冠心病组和对照组的分布相关性依然不存在.结论 TNFRSF1B基因rs1061624位点的等位基因A/G与中国人群冠心病发病不相关. 相似文献
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Background and Aims: Coronary artery disease (CAD) is a major cause of morbidity and mortality in patients with non-alcoholic fatty liver disease (NAFLD). Previous studies have suggested that TCF7L2 rs7903146 was related to the risk of developing NAFLD but the conclusions are not consistent and no related study has been conducted in Chinese populations. The aim of this study was to investigate the association between TCF7L2 rs7903146 and the risk of developing NAFLD and CAD in a Chinese Han population.Methods: TCF7L2 rs7903146 genotypes were measured by the MALDI-TOF-MS from 143 NAFLD patients, 159 CAD patients, 131 NAFLD + CAD patients, and 212 healthy controls. The demographic data and serum lipid profiles of all subjects were collected. The distributions of genotype and allele frequency in each group were also tested. Logistic regression was used to investigate the risk of TCF7L2 rs7903146 with NAFLD and CAD. All statistical analyses were conducted using SPSS 23.0.Results: There were no significant differences in the distributions of TCF7L2 rs7903146 genotype and allele frequency in each of the two groups, and the TCF7L2 rs7903146 CT + TT genotype did not increase the risk of developing NAFLD, CAD, and NAFLD + CAD. Except for body mass index in the control group, the differences of clinical parameters between the TCF7L2 rs7903146 T allele carriers and non-carriers in each group were not significant. In the non-obese group, the TCF7L2 rs7903146 CT + TT genotype was a protective factor for the development of NAFLD in the non-obese subjects (odds ratio=0.359, 95% confidence interval: 0.134-0.961, p = 0.041).Conclusions: TCF7L2 rs7903146 was not associated with the risk of developing NAFLD, CAD, and NAFLD + CAD in the Chinese Han population. In the non-obese population, the TCF7L2 rs7903146 CT + TT genotype was a protective factor against the development of NAFLD. 相似文献
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目的 研究Fractalkine受体CX3CR1基因多态性(249V/I和280T/M)与冠心病的相关性.方法 应用聚合酶链反应限制片长多态性方法对139例冠心病患者和90例对照者的CX3CR1基因多态性进行分析,比较CX3CR1基因多态性在两组之间的差异性.结果 等位基因I249在对照组中的分布频率明显高于冠心病组(P<0.05);冠心病组280T/M基因型和等位基因频率分布与对照组比较无显著性差异(P>0.05).结论 Fractalkine受体CX3CR1等位基因I249变异可能与冠心病的发病危险性下降有关,CX3CR1基因多态性与中国南方汉族人群冠心病的发生存在相关性. 相似文献
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Mohammad Hashemi Hamideh Hanafi Bojd Ebrahim Eskandari Nasab Ali Bahari Noor Allah Hashemzehi Sara Shafieipour Behzad Narouie Mohsen Taheri Saeid Ghavami 《Hepatitis monthly》2013,13(5)
Background
Genetic and environmental factors are important for the development of nonalcoholic fatty liver disease (NAFLD). Adiponectin is a white and brown adipose tissue hormone, and have been found to play essential roles in the regulation of energy homoeostasis. Recent reports have identified a possible role of adiponectin in NAFLD via PPARγ pathway.Objectives
The present study was designed to find out the impact of adiponectin rs1501299 (276G/T) and rs266729 (-11377C/G) gene polymorphisms in NAFLD.Patients and Methods
Eighty-three patients with diagnosis of NAFLD, and 93 healthy subjects were included in the study. Tetra ARMS-PCR was designed to detect single nucleotide polymorphisms.Results
A significant difference was found between NAFLD and control group regarding the rs266729 polymorphism (χ2 = 7.35, P = 0.025). The rs266729 polymorphism increased the risk of NAFLD in codominant (CC vs. CG: OR = 2.18, 95% CI = 1.16 - 4.12, P = 0.016) and dominant (CC vs. CG/GG: OR = 2.31, 95% CI = 1.25 - 4.27; P = 0.008) inheritance tested models. The G allele increased the risk of NAFLD (OR = 1.63, 95% CI = 1.03 - 2.57, P = 0.037) in comparison with C allele. No significant difference was found between the groups concerning adiponectin rs1501299 gene polymorphism (χ2 = 0.70, P = 0.697).Conclusions
adiponectin rs266729 polymorphism might be a candidate gene, which determines the susceptibility to NAFLD. Larger studies are necessary to confirm these findings in various populations. 相似文献10.
Zeynab Shaghaghi Mortaza Bonyadi Mohammad H. Somi Manouchehr Khoshbaten 《Saudi Journal Of Gastroenterology》2014,20(1):54-58
Background/Aim:
Previous studies have shown the association of some genetic factors, such as Plasminogen activator inhibitor type-1 (PAI-1) 4G/5G polymorphism, with the development of inflammatory bowel disease (IBD). We aimed to study this polymorphism as a risk factor in IBD patients in this cohort.Patients and Methods:
One hundred and fifteen IBD patients and 95 healthy controls were selected from Iranian Azeri Turks and -6754G/5G polymorphism of PAI-1 gene was tested by polymerase chain reaction using allele-specific primers confirmed by sequencing.Results:
There was no significant difference of PAI-1 polymorphism between IBD patients and the control group (P > 0.05). Furthermore, these data showed no significant difference between Crohn''s disease and ulcerative colitis patients. However, 4G/4G homozygotes have reduced probability to progression of loss of appetite, whereas 5G/5G genotypes have increased risk for development of chronic diarrhea without blood, nausea, and loss of appetite.Conclusions:
Although our study showed no significant association of PAI-1 polymorphism between patients and control group, the carriers of 4G/4G genotype and 4G allele had reduced risk for the progression of IBD features in this cohort. 相似文献11.
背景:前列腺干细胞抗原(PSCA)基因多态性与日本人群弥漫型胃癌的发生相关。目的:探讨PSCA rs2294008多态性与中国汉族人群胃癌发病、胃癌临床病理特征和患者预后之间的关系。方法:以PCR-RFLP方法判定460例胃癌患者和549例健康对照者的PSCA rs2294008基因型,分析rs2294008基因型与胃癌发病风险和胃癌临床病理特征之间的关系并进行生存分析。结果:病例组与对照组的PSCA rs2294008基因型分布频率差异有统计学意义(P=0.018)。与CC基因型相比.携带T等位基因(TT/TC)显著增加胃癌的发病风险(OR=1.42,95% CI:1.10~1.82,P=0.006)。进一步按胃癌临床病理特征进行分层分析,携带T等位基因显著增加肠型(OR=1.55,95% CI:1.18~2.04,P=0.002)、低分化(OR=1.59,95% CI:1.19~2.13,P=0.002)、非贲门(OR=1.55,95% CI:1.17~2.04,P=0.002)胃癌的发病风险。Cox回归分析显示TT基因型(HR=2.12,95% CI:1.22~3.69,P=0.008)和肿瘤的TNM分期是影响胃癌患者预后的危险因素。结论:PSCA rs2294008 T等位基因与中国汉族人群的胃癌发病有关,可增加肠型、低分化、非贲门胃癌的发病风险,TT基冈型是影响胃癌患者预后的危险因素之一。 相似文献
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Chen Yuan Linlin Lu Baiquan An Wenwen Jin Quanjiang Dong Yongning Xin Shiying Xuan 《Hepatitis monthly》2015,15(12)
Background:
Recent genome-wide association studies (GWAS) identified that gene Lysophospholipase-like 1 (LYPLAL1) rs12137855 associated with non-alcoholic fatty liver disease (NAFLD). No research has been performed regarding the association between LYPLAL1 and NAFLD in China.Objectives:
The aim of the present study was to investigate the association between the gene LYPLAL1 rs12137855 and NAFLD, and the effect on serum lipid profiles in a Chinese Han population.Patients and Methods:
LYPLAL1 rs12137855 gene was genotyped in 184 patients with NAFLD and 114 healthy controls using sequencing and polymerase chain reaction analysis (PCR). We tested serum lipid profiles using biochemical methods.Results:
No significant differences in genotype and allele frequencies of LYPLAL1 rs12137855 was found between the NAFLD group and the controls group (P > 0.05). Subjects with the variant LYPLAL1 rs12137855 CC genotype had a higher mean weight, body mass index (BMI) and low density lipoprotein (LDL).Conclusions:
Our results showed for the first time that LYPLAL1 gene is not associated with a risk of NAFLD development in the Chinese Han population. The variant carriers of overall subjects significantly increased weight, BMI and LDL. 相似文献13.
Shi‐Kun Yang Na Song Yang‐Yang Wang Ai‐Mei Li Jun Liu Fei Deng Ming Zhan Wei Zhang Ya‐Chun Han Hao Zhang 《Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy》2019,23(5):425-436
This study aimed to explore the relationship between vitamin D receptor (VDR) gene polymorphisms and the risk of nephrolithiasis. All relevant trials were searched from multiple databases according to predefined criteria, the pooled OR and corresponding 95% CI were analyzed using Stata software. Seventeen studies involving 2441 cases and 2296 controls were included. The pooled analysis showed that VDR BsmI, FokI, and ApaI gene polymorphisms were not associated with nephrolithiasis susceptibility either in Asian and in Caucasians populations. VDR TaqI gene polymorphism was associated with nephrolithiasis in the overall populations (T vs. t: OR = 0.84, 95% CI: 0.73–0.95, P = 0.006; TT vs. Tt + tt: OR = 0.79, 95% CI: 0.66–0.95, P = 0.010). In Asian population, VDR TaqI gene polymorphism also was associated with nephrolithiasis susceptibility (TT vs. Tt + tt: OR = 0.72, 95% CI: 0.55–0.93, P = 0.012; Tt vs. TT + tt: OR = 1.43, 95% CI: 1.00–2.05, P = 0.048). But TaqI gene polymorphism was not associated with nephrolithiasis risk in Caucasian populations (T vs. t: OR = 0.85, 95% CI: 0.72–1.00, P = 0.051; TT vs. Tt + tt: OR = 0.87, 95% CI: 0.68–1.10, P = 0.245; tt vs. Tt + TT: OR = 1.32, 95% CI: 0.86–2.01, P = 0.206; Tt vs. TT+ tt: OR = 0.98, 95% CI: 0.70–1.38, P = 0.931). VDR BsmI, FokI, and ApaI gene polymorphisms were not associated with the risk of nephrolithiasis either in Asian and Caucasians populations, but VDR TaqI gene polymorphism was associated with nephrolithiasis in the Asian subjects. 相似文献
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ABCA1基因多态性与冠心病的相关研究 总被引:1,自引:0,他引:1
目的探讨三磷酸腺苷结合盒转运子A1(ATP binding cassette transporter1,ABCA1)基因R219K多态性与冠心病(coronary heart disease,CHD)易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测了162例冠心病患者和186名对照的ABCA1基因R219K基因型和等位基因的分布。结果两组基因型频率均符合Hardy-Weinberg平衡。对照组的RK和KK基因型的频率显著高于CHD组(73.66%比56.17%,P<0.05),同样,对照组K等位基因的频率显著高于CHD组(48.66%比36.11%,P<0.01)。调整年龄、性别、体重指数、吸烟和高血压等因素后,携带至少1个219K等位基因的个体显著减少患冠心病的危险(OR=0.38;95%CI=0.22-0.65)。结论ABCA1基因R219K多态性与中国温州汉族人群冠心病的遗传易感性相关,其中K等位基因可能是冠心病的保护因子。 相似文献
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肾素-血管紧张素系统基因多态性与冠心病的关系 总被引:1,自引:0,他引:1
目的探讨肾素-血管紧张素系统三个关键基因血管紧张素转化酶基因插入/缺失多态性、血管紧张素原基因M235T多态性及血管紧张素Ⅱ1型受体基因A1166/C多态性与冠心病的关系。方法应用多聚酶链反应-限制片长多态性方法对110例冠心病患者和80例健康人分别进行单基因和基因连锁分析。结果①冠心病组血管紧张素转化酶基因DD基因型(43.6%)及D等位基因频率(60.5%)明显高于正常对照组(分别为26.3%和44.4%,P<0.05);血管紧张素原基因TT基因型(66.4%)及T等位基因频率(78.6%)亦明显高于正常对照组(分别为42.5%和60.6%,P<0.05);与正常对照组相比,冠心病组血管紧张素Ⅱ1型受体基因的AA、AC基因型频率和A、C等位基因频率差异均无显著性(P>0.05)。②联合分析三个基因多态性罹患冠心病的相对风险,其OR为3.395,高于单基因血管紧张素转化酶DD型(OR为2.175)及血管紧张素原TT型(OR为2.669),低于血管紧张素转化酶DD型 血管紧张素原TT型(OR为6.098)。结论血管紧张素转化酶基因插入/缺失多态性及血管紧张素原基因M235T多态性与冠心病有关,而血管紧张素Ⅱ 1型受体基因A1166/C多态性可能与冠心病无关联。同时具有血管紧张素转化酶DD型及血管紧张素原TT型者发生冠心病的相对风险显著增高。 相似文献
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Filipe Carvalho Marmelo Sónia Matilde Fonseca Mateus Alexandre José Marques Pereira 《Arquivos brasileiros de cardiologia》2014,103(5):398-402
Background
Aortic valve sclerosis (AVS) is characterized by increased thickness, calcification and stiffness of the aortic leaflets without fusion of the commissures. Several studies show an association between AVS and presence of coronary artery disease.Objective
The aim of this study is to investigate the association between presence of AVS with occurrence of previous coronary artery disease and classical risk factors.Methods
The sample was composed of 2,493 individuals who underwent transthoracic echocardiography between August 2011 and December 2012. The mean age of the cohort was 67.5 ± 15.9 years, and 50.7% were female.Results
The most frequent clinical indication for Doppler echocardiography was the presence of stroke (28.8%), and the most common risk factor was hypertension (60.8%). The most prevalent pathological findings on Doppler echocardiography were mitral valve sclerosis (37.1%) and AVS (36.7%). There was a statistically significant association between AVS with hypertension (p < 0.001), myocardial infarction (p = 0.007), diabetes (p = 0.006) and compromised left ventricular systolic function (p < 0.001). Conclusion: Patients with AVS have higher prevalences of hypertension, stroke, hypercholesterolemia, myocardial infarction, diabetes and compromised left ventricular systolic function when compared with patients without AVS. We conclude that there is an association between presence of AVS with previous coronary artery disease and classical risk factors. 相似文献17.
目的 本研究旨在探讨Apo C-1基因rs4420638多态性与颈动脉内膜中层厚度(IMT)和斑块的关系。方法 研究纳入北京石景山队列人群中无心肌梗死、脑卒中和癌症病史且资料完整者1 345人 (男性457人,女性888人,年龄41~78岁之间)。采用标准方法进行心血管病危险因素调查,用彩色多普勒超声仪检测颈动脉IMT及斑块,SNaPshot法检测ApoC-1基因rs4420638多态性。结果 采用显性模型,将基因型分为AA和GA+GG两组。GA+GG组血清高敏C反应蛋白(hs-CRP)显著低于AA组(P<0.05);两组间TC、TG、LDLC,HDLC差异无显著性(P>0.05)。单因素和多因素方差分析两组间颈动脉IMT差异无显著性(P>0.05)。单因素和多因素logistics回归分析(调整年龄、性别;调整年龄、性别和传统危险因素),GA+GG组斑块检出率显著低于AA组(P0.02),而在多因素模型基础上进一步调整hs-CRP,关联无统计学意义(P>0.05)。结论 本研究人群中ApoC-1基因rs4420638与血浆hs-CRP显著相关,而与颈动脉IMT、颈动脉斑块率(调整hs-CRP)均无显著关联。 相似文献
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Polymorphism of Motilin Gene in Patients with Crohn's Disease 总被引:2,自引:0,他引:2
Vito Annese Ada Piepoli Angelo Andriulli Grazia Napolitano Luigi Bisceglia Leopoldo Zelante Paolo Gasparini 《Digestive diseases and sciences》1998,43(4):715-719
An increasing body of evidence supports theconcept of genetic heterogeneity within inflammatorybowel disease (IBD). In this study, a polymorphism ofthe motilin gene, which determines an amino acidsubstitution in the motilin protein, has been investigatedin IBD patients. Fifty patients with ulcerative colitis(UC), and 52 with Crohn's disease (CD) were investigatedfor anti-neutrophil cytoplasmatic antibodies (ANCA) and the polymorphism in the second exonof the motilin gene. Sixty unrelated blood donors servedas controls. ANCA were found in 30% of UC and 13% of CD.In controls the DNA polymorphism identified two alleles (1 and 2) at a frequency of 42% and58%, respectively. Patients with either UC or CD showeda slight increase in the frequency of allele 2 (69% and60%, respectively; P > 0.05 vs controls). This allele was predominant in ANCA-positive CDpatients (86%; P < 0.04) while in UC it did notdiffer. All ANCA-positive CD patients had the diseaseconfined to the colon. A polymorphism of second exon of the motilin gene, leading to a proteinvariant, is significantly more frequent in the subset ofANCA-positive CD patients. This subgroup of patientsappears to share peculiar genetic and clinical features. 相似文献
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Sheng Peng Zhongshen Kuang Chenyi Sheng Yan Zhang Hang Xu Qinghong Cheng 《Digestive diseases and sciences》2010,55(8):2288-2293
Background
It has been proposed that single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) could affect the expression of the miRNA and contribute to the susceptibility of human tumors. However, the role of genetic variant (T/C) in miR-196a-2 in gastric cancer susceptibility is still unknown. 相似文献20.
Ryszard T. Smolenski Iwona Rybakowska Jacek Turyn Paweł Romaszko Magdalena Zabielska Anne Taegtmeyer Ewa M. Słomińska Krystian K. Kaletha Paul J. R. Barton 《Cardiovascular drugs and therapy / sponsored by the International Society of Cardiovascular Pharmacotherapy》2014,28(2):183-189
Nucleotide metabolism and signalling is directly linked to myocardial function. Therefore analysis how diversity of genes coding nucleotide metabolism related proteins affects clinical progress of heart disease could provide valuable information for development of new treatments. Several studies identified that polymorphism of AMP deaminase 1 gene (AMPD1), in particular the common C34T variant of this gene was found to benefit patients with heart failure and ischemic heart disease. However, these findings were inconsistent in subsequent studies. This prompted our detailed analysis of heart transplant recipients that revealed diverse effect: improved early postoperative cardiac function associated with C34T mutation in donors, but worse 1-year survival. Our other studies on the metabolic impact of AMPD1 C34T mutation revealed decrease in AMPD activity, increased production of adenosine and de-inhibition of AMP regulated protein kinase. Thus, genetic, clinical and biochemical studies revealed that while long term attenuation of AMPD activity could be deleterious, transient inhibition of AMPD activity before acute cardiac injury is protective. We suggest therefore that pharmacological inhibition of AMP deaminase before transient ischemic event such as during ischemic heart disease or cardiac surgery could provide therapeutic benefit. 相似文献