共查询到20条相似文献，搜索用时 31 毫秒
1.
Aaron B.C. Wong Michael T.M. Wang Kevin Liu Zak J. Prime Helen V. Danesh-Meyer Jennifer P. Craig 《The ocular surface》2018,16(3):289-293
Purpose
To assess tear film parameters, ocular surface characteristics, and dry eye symptomology in patients receiving topical anti-glaucoma medications.Methods
Thirty-three patients with a diagnosis of open angle glaucoma or ocular hypertension, receiving unilateral topical anti-glaucoma medication for at least 6 months, were recruited in a cross-sectional, investigator-masked, paired-eye comparison study. Tear film parameters, ocular surface characteristics, and dry eye symptomology of treated and fellow eyes were evaluated and compared.Results
The mean?±?SD age of the participants was 67?±?12 years, and the mean?±?SD treatment duration was 5.3?±?4.4 years. Treated eyes had poorer non-invasive tear film breakup time (p?=?0.03), tear film osmolarity (p?=?0.04), bulbar conjunctival hyperaemia (p?=?0.04), eyelid margin abnormality grade (p?=?0.01), tear meniscus height (p?=?0.03), and anaesthetised Schirmer value (p?=?0.04) than fellow eyes. There were no significant differences in dry eye symptomology, meibomian gland assessments, and ocular surface staining between treated and fellow eyes (all p?>?0.05).Conclusions
Adverse changes in tear film stability, tear osmolarity, conjunctival hyperaemia, and eyelid margins were observed in treated eyes. This suggests that inflammatory mechanisms may be implicated in the development of dry eye in patients receiving long term topical anti-glaucoma therapy. 相似文献2.
Yael Kusne M&#x;hamed Temkit Nandita Khera Dharmendra R. Patel Joanne F. Shen 《The ocular surface》2017,15(4):784-788
Purpose
Dry eye symptoms greatly impact patients' quality of life in ocular graft-versus-host disease (oGVHD). Various ocular surface changes have been reported in oGVHD, including meibomian gland atrophy (MGA) and clinical conjunctival scarring or subepithelial fibrosis (CSEF). The relationships between CSEF, MGA, and other ocular surface changes in oGVHD were examined.Methods
Charts of 21 consecutive GVHD patients examined by a single ophthalmologist were retrospectively reviewed. International Chronic Ocular Graft-vs-Host-Disease Consensus Group (ICCG) scores were calculated for each patient using previously published methods. The severity of CSEF by slit lamp examination and MGA by infrared meibography were also assessed for each patient. Infrared meibography images were analyzed using ImageJ to determine percent of MGA. Pearson correlation coefficients were calculated using SAS Studio 9.3 (SAS Institute, Cary, NC).Results
In the 42 eyes, no significant correlations were identified among the variables examined (CSEF score, ICCG score, MGA). Further examination revealed asymmetric ocular findings in 20 of 21 patients. Analysis of the more severe eye alone (n = 21) revealed a weakly positive correlation between ICCG score and CSEF (r = 0.54; p = 0.01). No other statistically significant correlations were found.Conclusions
Clinical CSEF may be an important sign of GVHD impact on the ocular surface and may be relevant in oGVHD severity assessment. Though meibomian glands and conjunctiva are in close proximity, MGA did not correlate with clinical CSEF findings. Some ocular GVHD patients may present with asymmetrical ocular findings, with one eye displaying more severe pathological changes and symptoms despite the systemic nature of GHVD. Further studies are needed to examine these findings. 相似文献3.
Xuhua Tan Yihe Chen William Foulsham Afsaneh Amouzegar Takenori Inomata Yizhi Liu Sunil K. Chauhan Reza Dana 《The ocular surface》2018,16(4):470-477
Purpose
In this study, we examine the expression of corneal epithelium-derived thrombospondin-1 (TSP-1) and its immunomodulatory functions in a validated murine model of dry eye disease (DED).Methods
DED was induced in female C57BL/6 using a controlled environment chamber (CEC) for 14 days. mRNA and protein expression of TSP-1 by corneal epithelial cells was quantified using real-time PCR and flow cytometry. Corneal epithelial cells from either naïve or DED mice were cultured with bone marrow derived dendritic cells (BMDCs) in the presence of IFNγ for 48?h, and BMDC expression of MHC-II and CD86 was determined using flow cytometry. Next, either recombinant TSP-1 or anti-TSP-1 antibody was added to the co-culture, and BMDC expression of above activation markers was evaluated. Finally, either DED mice were topically treated with either recombinant TSP-1 or human serum albumin (HSA), and maturation of corneal DCs, expression of inflammatory cytokines, and DED severity were investigated.Results
mRNA expression of TSP-1 by the corneal epithelium was upregulated in DED. Corneal epithelial cells derived from mice with DED demonstrated an enhanced capacity in suppressing BMDC expression of MHC-II and CD86 relative to wild type mice, and this effect was abrogated by TSP-1 blockade and potentiated by recombinant TSP-1. Finally, topical application of recombinant TSP-1 significantly suppressed corneal DC maturation and mRNA expression of pro-inflammatory cytokines, and ameliorated disease severity in mice with DED.Conclusions
Our study elucidates the function of epithelium-derived TSP-1 in inhibiting DC maturation and shows its translational potential to limit corneal epitheliopathy in DED. 相似文献4.
Jun Zhang Carolyn G. Begley Ping Situ Trefford Simpson Haixia Liu 《The ocular surface》2017,15(4):696-703
Purpose
We used the presence or absence of a soft contact lens (CL) as a barrier to test the hypothesis that tear breakup (TBU) presents a direct noxious stimulus to the ocular surface.Methods
Ten subjects kept one eye open as long as possible, termed sustained tear exposure (STARE), for 10 consecutive trials while discomfort was monitored with and without a CL in place. The area of TBU was quantified in each frame. Discomfort was measured during and after each STARE trial and symptoms of ocular irritation were assessed before and after all testing.Results
TBU increased at the end of trials to an average of 19.89% ± 17.91% and 20.58% ± 15.33% and discomfort to 9.09 ± 1.44 and 1.97 ± 2.19 in trials without and with a CL, respectively. Discomfort was significantly higher during trials without CLs (Friedman test, p < 0.005), but there was no significant difference in the area of TBU between trials (Friedman test, p = 0.296) with and without a CL (Friedman test, p = 0.527). Discomfort after each STARE trial increased significantly across trials (Friedman, p < 0.005). Symptoms of ocular irritation increased significantly from pre- to post-testing (Wilcoxon signed rank test, p < 0.005).Conclusions
TBU during STARE trials was associated with increasing ocular discomfort, which was partially blocked by wearing a CL, supporting the hypothesis that TBU directly stimulates the corneal surface. Repeated STARE trials led to increasing discomfort and dry-eye like symptoms of ocular irritation, suggesting that repeated bouts of TBU can lead to alterations in ocular surface sensory processing. 相似文献5.
Michael T.M. Wang Leslie Tien Alicia Han Jung Min Lee Dabin Kim Maria Markoulli Jennifer P. Craig 《The ocular surface》2018,16(4):424-429
Purpose
To investigate the influence of blinking on tear film parameters, ocular surface characteristics, and dry eye symptomology.Methods
A total of 154 participants were recruited in an age, gender and ethnicity-matched cross-sectional study, of which 77 exhibited clinically detectable incomplete blinking, and 77 did not. Blink rate, dry eye symptomology, tear film parameters, and ocular surface characteristics were assessed in a single clinical session.Results
Overall, a higher proportion of participants exhibiting incomplete blinking fulfilled the TFOS DEWS II dry eye diagnostic criteria (64% versus 44%, p?=?0.02), with an odds ratio (95% CI) of 2.2 (1.2–4.2) times. Participants exhibiting incomplete blinking had higher Ocular Surface Disease Index scores (18?±?13 versus 12?±?9, p?=?0.01), and greater levels of meibomian gland dropout (41.3?±?15.7% versus 27.5?±?14.1%, p?<?0.001). Furthermore, poorer tear film lipid layer thickness, non-invasive tear film stability, expressed meibum quality, eyelid notching, and anterior blepharitis grades were also observed in those exhibiting incomplete blinking (all p?<?0.05). Blink frequency did not correlate significantly with any ocular surface parameters (all p?>?0.05).Conclusions
Incomplete blinking was associated with a two-fold increased risk of dry eye disease. The greater levels of meibomian gland dropout, as well as poorer expressed meibum quality and tear film lipid layer thickness, observed would suggest that incomplete blinking may predispose towards the development of evaporative dry eye. 相似文献6.
Mohsen Bahmani Kashkouli Sayyed Amirpooya Alemzadeh Hossein Aghaei Farzad Pakdel Parya Abdolalizadeh Mahya Ghazizadeh Farideh Moradpasandi 《The ocular surface》2018,16(4):458-462
Purpose
To compare the subjective versus Objective dry eye disease (DED) in patients with moderate-severe thyroid eye disease (TED).Method
Included were the patients with moderate-severe TED and ≥18 years old. They completed the ocular surface disease index (OSDI) questionnaire and had Schirmer, Tear breakup time (TBUT), fluorescein staining, osmolarity, corneal aesthesiometry, and meibomian gland dysfunction (MGD) tests. Excluded were patients with history of any disease, surgery and or medications which might be affecting the ocular surface and incomplete tests results. Subjective DED was defined as OSDI score of ≥13 and objective as one abnormal sign (TBUT, Schirmer, Osmolarity, and Staining). Presence of both was defined as definite DED.Results
Included were 38 patients (74 eyes) with mean age of 40 years. Subjective DED was detected in 77%, objective in 89.2%, and definite in 67.7% of the eyes. Severe subjective and objective DED were found in 36.5% and 24.3% of the eyes, respectively. TBUT was the most frequent positive test (63.5%). MGD was observed in 56.8% of the eyes. Mean clinical activity score, palpebral fissure, rundle grading, proptosis, corneal aesthesiometry, and presence of MGD were not significantly different between the eyes with and without subjective, objective, or definite DED.Conclusion
Definite DED was found in more than 2/3 of the eyes with moderate-severe TED. While frequency of objective DED was higher, severe form of subjective DED was more frequent. No variable was significantly different between the eyes with and without subjective, objective and definite DED. 相似文献7.
Arthur Ferrero Seydou Alassane Christine Binquet Lionel Bretillon Niyazi Acar Louis Arnould Aurore Muselier-Mathieu Cécile Delcourt Alain M. Bron Catherine Creuzot-Garcher 《The ocular surface》2018,16(1):112-119
Purpose
To estimate the prevalence of dry eye disease (DED) in the elderly and to describe systemic and ocular factors associated with DED in a population-based study, the Montrachet (Maculopathy Optic Nerve nuTRition neurovAsCular and HEarT diseases) study.Methods
Dry eye symptoms were evaluated using the Ocular Surface Disease Index (OSDI) questionnaire. A self-reported history of dry eye symptoms and artificial tear use were also recorded. Every participant underwent an ophthalmic evaluation, which included the Schirmer test without anesthesia (Schirmer I), tear film breakup time (TFBUT) measurement, and fluorescein corneal staining evaluation.Results
A total of 1045 subjects were included in the study. The mean age was 82.2 ± 3.8 years. The prevalence of DED according to symptoms, signs, or both was 34.4% [95% confidence interval ({CI)} 31.5–37.2], 34.5% [95% CI 31.6–37.3] and 13.0% [95% CI 10.9–15.0], respectively. Compared with men, women were more likely to self-report DED and artificial tear use, more often displayed an OSDI >22, and more often suffered from subjective and definite DED. In multivariate analysis, associated factors with definite DED were an educational level of short secondary school (to age 15 years), best-corrected visual acuity <20/60, dark iris color, systemic hypertension, and anxiolytic agent and antihistamine eye drop use.Conclusion
Dry eye disease is a major ophthalmologic condition with a high prevalence among the elderly. We report well-documented factors associated with DED and new associations deserving further investigation. 相似文献8.
Luigi Berchicci Alessandro Rabiolo Alessandro Marchese Lorenzo Iuliano Carmen Gigliotti Elisabetta Miserocchi Francesco Bandello Giulio Modorati 《The ocular surface》2018,16(3):314-321
Purpose
This study aimed to assess the prevalence and clinical manifestations of ocular graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods
269 patients who received allo-HSCT for hematologic malignancies were evaluated between December 2013 and April 2017 in this prospective observational study. Subjects underwent ophthalmologic examination at 6, 12 and 24 months after allo-HSCT. We evaluated the ophthalmologic and hematological data using the NIH consensus criteria definition and the International Chronic Ocular GVHD Consensus Group scoring systems.Results
According to NIH consensus criteria definition, ocular GVHD developed in 46.1%, 51.9% and 54.7% of patients at the follow-up visits. According to the International Chronic Ocular GVHD Consensus Group, ocular GVHD developed in 41.3%, 47.7% and 51.9% of patients at the follow-up visits. We found a strong association between the presence of systemic GVHD and the development of chronic ocular GVHD throughout the entire follow-up period. Weaker associations were found between the presence of a female donor, matched related donor, conjunctival hyperemia and conjunctival fibrosis.Conclusions
More than 50% of subjects develop chronic ocular GVHD after allo-HSCT. The presence of active systemic GVHD, female donor and matched related donor are associated with ocular GVHD development. 相似文献9.
10.
Purpose
To investigate the feasibility of remote assessment and follow-up of dry eye symptoms using electronic versions of two validated questionnaires.Methods
We conducted a prospective study of consecutive patients with dry eye disease (DED). Patients were enrolled during a clinical visit and were explained how to respond electronic versions of the Ocular surface Disease Index (OSDI) and the Symptom Assessment in Dry Eye (SANDE) questionnaires using a computer in the presence of investigators. A secure link to both questionnaires was sent to each patient every 2 weeks in order to respond and submit their symptoms over a 3-month period.We analyzed the number of patients who responded to both questionnaires, the recurrence, and the symptoms scores reported.Results
A total of 1121 questionnaires were collected; 103 patients (85%) reported their symptoms at least once during the 3-month study duration. The majority of participants who completed the study (71.6%) responded remotely at least once per month during the 3-month duration of the study. The mean OSDI and SANDE scores from the total of remote evaluations were 34.9?±?21.9 (range 0–97.5) and 50.3?±?24.9 (range 0–100), respectively. There was a statistically significant correlation between the total scores collected with the two questionnaires (R?=?0.67, P?<?0.001).Conclusions
Patients are motivated to report DED symptoms while away from the clinic. Distance-based evaluation of DED symptoms is both feasible and convenient, and can be implemented to follow symptoms in large populations with chronic dry eye. 相似文献11.
Sridevi Nair Murugesan Vanathi Manoranjan Mahapatra Tulika Seth Jasbir Kaur T. Velpandian Alok Ravi Jeewan Singh Titiyal Radhika Tandon 《The ocular surface》2018,16(3):352-367
Aim
To analyze tear cytokines levels and their correlation to ocular surface parameters in allogenic hematopoietic stem cell transplants (allo-HSCT) patients.Methods
Prospective longitudinal study of allo-HSCT patients and controls for ocular surface evaluation (OSDI, TBUT, Schirmer's test, staining scores), tear biochemical analysis for protein, cytokines [IL-10, IL-12, IL-2, IL-4, IL-6, IL-17, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, VEGF], MMPs [MMP 2, 9, 7, 13, 10 and chemokine (IL-8)], & VEGF on three consecutive follow up visits (at three monthly interval) was done.Results
Of 24 post allo-HSCT patients (19 males, 5 females) & 12 controls (mean age 34.3 + 5.8 years) enrolled, 20 patients [mean age 33.4 + 7.77 years; mean time of recruitment of 5.2 + 2.12 months following alloHSCT] who completed three consecutive follow up visits were included for analysis. Ocular GVHD (oGVHD) was seen in 8 patients (33.3%). Tears biochemical analysis showed elevated levels of interferon γ, IL 6, IL 8, IL 10, IL 12AP70, IL 17A, MMP 9 and VEGF in oGVHD eyes as compared to non-oGVHD & control eyes. Non-oGVHD eyes showed elevated tear MMP 7 and MMP 9 as compared to healthy controls. Tear protein levels were significantly decreased in oGVHD eyes and were equivocal in nonGVHD and control eyes. TBUT and ocular staining scores to correlate best with tear interleukins and MMPs.Conclusion
Evaluation of levels of tear VEGF, total protein & MMP 9 can be of significance in identifying oGVHD in post alloHSCT patients. 相似文献12.
Purpose
To perform a bibliometric analysis in the field of dry eye disease (DED) research to characterize the current international status of DED research and to identify the most effective actors (journals, countries, authors) involved in this field.Methods
Scientometric methods were used to evaluate global scientific production and development trends in DED research, using the Web of Science Core Collection.Results
The growth of the literature related to DED averaged 12.18% over the last 10 years. A total of 5522 original and review articles, published in 821 different journals, were identified. The USA was the most productive country with 34.53% of the overall articles studied and 46.10% of the overall citations. The Ocular Surface published a very high percentage of articles related to DED relative to the total number of articles published (31.87%). The most productive institutions and the most frequently cited articles were from the USA and Japan. A network visualization map for country collaboration revealed that most European countries developed most of their collaborations with countries belonging to their own continent, which was not the case for the USA or Japan. A total of 41,956 KeyWords Plus were found with an average of 7.6 (SD = 3.15) KeyWords Plus per article.Conclusions
This study provides a broad view of the current status and trends in DED research and may help clinicians, researchers and policy makers better understand this research field and predict its dynamic directions. 相似文献13.
Louis Tong Lei Zhou Roger Beuerman Susan Simonyi David A. Hollander Michael E. Stern 《The ocular surface》2017,15(4):736-741
Purpose
To investigate effects of punctal occlusion on global tear protein levels in patients with dry eye.Methods
In this prospective, longitudinal, single-center study, nonabsorbable punctal plugs were inserted bilaterally into the lower punctum of 30 patients with moderate dry eye. Dry eye symptoms, fluorescein corneal staining, Schirmer I test, tear film break-up time, and safety were assessed in the more severely affected eye. Tear proteins at weeks 1 and 3 were quantified by iTRAQ relative to baseline preocclusion levels.Results
Of 29 patients who completed the study, 23 (mean age 49.8 years) had sufficient tear samples for analysis. After 3 weeks, punctal occlusion significantly upregulated tear proteins, including glutathione synthase (mean of 1.6-fold, P = 0.01) and interleukin-1 receptor antagonist (1.7-fold, P = 0.032) and downregulated cholinergic receptor (neuronal) alpha-7 (0.79-fold, P = 0.039) and lymphocyte cytosolic protein-1 (0.66-fold, P = 0.012). Clustering analysis of global tear proteins revealed two clear profile changes; the first group of patients (cluster 1, n = 10) had a reduction in the inflammatory proteins (e.g., S100A8) and rise in lacrimal proteins supporting the ocular surface (e.g., lysozyme), whereas the second group (cluster 2, n = 13) had an increase in inflammatory proteins and a decrease in lacrimal proteins. Logistic regression analysis revealed that cluster 1 patients had significantly (P = 0.006) lower Schirmer scores at baseline (mean [standard deviation]: 4.3 [4.3] mm) than cluster 2 (6.8 [2.6] mm).Conclusions
Punctal plugs produced a beneficial pattern of tear protein change in patients with relatively low Schirmer scores within 3 weeks of punctal occlusion. 相似文献14.
15.
Jelle Vehof Nicole Sillevis Smitt-Kamminga Simone A. Nibourg Christopher J. Hammond 《The ocular surface》2018,16(2):242-248
Purpose
To investigate the role of sex on the symptomatology of DED and on the associations between symptoms and signs.Methods
A cross-sectional study was used including 755 dry eye patients from the Groningen Longitudinal Sicca Study (GLOSSY cohort). Patient symptoms were assessed by the Ocular Surface Disease Index (OSDI) questionnaire and dry eye signs by the six most commonly used tests. Patients were divided in groups based on overall severity of signs and within these groups total and specific symptoms were compared by sex. Sex differences in Spearman correlation between symptoms and signs were calculated.Results
Women had higher total symptom scores than men in both the mild (33.8 vs 24.7, P?=?.01) and moderate signs groups (38.3 vs 28.0, P?<?.005), but this difference was less apparent in the severe signs group (40.4 vs 37.2, P?=?.33). Independent of severity of signs, women consistently reported more light sensitivity than men (P?<?.01 in all groups). The correlation between symptoms and overall severity of signs score was significantly lower in women (ρ?=?0.11 vs ρ?=?0.33 in men, P?=?.01), with clearest differences between women and men in correlations with Schirmer (ρ?=?0.01 vs ρ?=?-0.21, P?=?.03) and TFBUT (ρ?=?-0.08 vs ρ?=?-0.30, P?=?.02).Conclusions
This large clinical study has shown that sex has a large influence on the symptomatology of DED, with significantly higher symptom scores and lower correlation between symptoms and signs in women compared to men. These findings are of importance in clinical practice and in conducting research into DED. 相似文献16.
Marwan Atallah Madhura Joag Anat Galor Guillermo Amescua Afshan Nanji Jianhua Wang Victor L. Perez Sander Dubovy Carol L. Karp 《The ocular surface》2017,15(4):688-695
Purpose
Coexistence of an ocular surface disease can mask the typical features of ocular surface squamous neoplasia (OSSN). The purpose of this study was to evaluate high resolution optical coherence tomography (HR-OCT) as an adjunct in the detection and differentiation of OSSN within coexisting ocular surface pathologies.Methods
Retrospective study of 16 patients with ocular surface disease and lesions suspicious for OSSN that were evaluated with HR-OCT. HR-OCT images of the lesions were taken to look for evidence of OSSN. Biopsies were performed in all cases, and the HR-OCT findings were compared to the histological results.Results
Of the 16 patients with OSSN and a coexisting ocular surface disease, 12 were found to have OSSN by HR-OCT and all were subsequently confirmed by biopsy. Two patients had OSSN with rosacea, one with pingueculum, two within pterygia, one with Salzmann’ nodular degeneration, six with limbal stem cell deficiency (LSCD)/scarring. In all 12 cases HR-OCT images revealed classical findings of hyper-reflective, thickened epithelium and an abrupt transition from normal to abnormal epithelium. OSSN was ruled out by HR-OCT in four cases (2 Salzmann's, 1 mucous membrane pemphigoid, and 1 LSCD). Negative findings were confirmed by biopsy. HR-OCT was used to follow resolution of the OSSN in positive cases, and it detected recurrence in 1 case.Conclusions
While histopathology is the gold standard in the diagnosis of OSSN, HR-OCT can be used to noninvasively detect the presence of OSSN in patients with coexisting ocular conditions. 相似文献17.
Shin Mizoguchi Hiroki Iwanishi Masahide Kokado Takayoshi Sumioka Geraint J. Parfitt Yilu Xie Reiko Arita Rika Shirakawa Osamu Yamanaka Yuka Okada James V. Jester Shizuya Saika 《The ocular surface》2017,15(4):713-722
Purpose
To examine effects of alkali injury of the ocular surface on meibomian gland pathology in mice.Methods
Three μL of 1 N NaOH were applied under general anesthesia to the right eye of 10-week-old BALB/c (n = 54) mice to produce a total ocular surface alkali burn. The meibomian gland morphology was examined at days 1, 2, 5, 10, and 20 by stereomicroscopy and non-contact infrared meibography. Mice were then sacrificed and eyelids processed for histology with hematoxylin-eosin and immunohistochemistry for ELOVL4, PPARγ, myeloperoxidase (a neutrophil marker) and F4/80 macrophage antigen, as well as TUNEL staining. Another set of specimens was processed for cryosectioning and Oil red O staining.Results
Alkali injury to the ocular surface produced cellular apoptosis, infiltration of neutrophils and macrophages, degeneration of the meibomian gland, and ductal dilation. Inflammation in and destruction of acunal stricture seemed more prominent in the lower eyelid, while duct dilation was more frequently observed in the upper eyelid during healing. Surviving acinar cells were labeled for ELOVL4 and PPARγ. Oil red O staining showed that the substance in the dilated duct contained predominantly neutral lipid.Conclusions
Alkali injury to the ocular surface results in damage and destruction of the eyelid meibomian glands. The pattern of the tissue damage differs between glands of the upper and lower eyelids. 相似文献18.
Purpose
Citation analysis represents one of the best currently available methods for quantifying the impact of articles. Bibliometric studies list the ‘‘best sellers’’ in a single field of interest. The purpose of the present study was to identify and analyze the most frequently cited papers in dry eye research that may be of high interest for researchers and clinicians.Methods
We reviewed the database of the Institute for Scientific Information to identify articles published from 1900 to September 2016. All dry eye articles published in 59 ophthalmology journals were identified. The top 100 articles were selected for further analysis of authorship, source journal, number of citations, citation rate, geographic origin, article type, and level of evidence.Results
The 100 most-cited articles were published between 1983 and 2011, with most of them in the 2000s. The number of citations per article ranged from 96 to 610, and was greatest for articles published in the 2000s. Each of these articles was published in one of 15 journals. Most articles represented Level-III evidence, followed by Levels II and I.Conclusions
The present study focusing on dry eye research revealed that 55% of the most-cited articles came from the U.S. and 18% from Japan. Diagnostics and therapy were the areas of focus of most of the clinical articles; 13% of the most cited papers were review articles. This analysis provides researchers and clinicians with a detailed overview on the most cited dry eye papers over the past decades. 相似文献19.
Fawzia Bardag-Gorce Richard Hoft Imara Meepe Julio Garcia Kumar Tiger Andrew Wood Amanda Laporte Derek Pan Andrew Makalinao Robert Niihara Joan Oliva Arjie Florentino Amber M. Gorce Jeremy Stark Daileen Cortez Samuel W. French Yutaka Niihara 《The ocular surface》2017,15(4):749-758
Purpose
This study focuses on characterizing proteasomes in corneal epithelial cells (CEC) and in cultured autologous oral mucosal epithelial cell sheets (CAOMECS) used to regenerate the ocular surface.Methods
Limbal stem cell deficiency (LSCD) was surgically induced in rabbit corneas. CAOMECS was engineered and grafted onto corneas with LSCD to regenerate the ocular surface.Results
LSCD caused an increase in inflammatory cells in the ocular surface, an increase in the formation of immunoproteasomes (IPR), and a decrease in the formation of constitutive proteasome (CPR). Specifically, LSCD-diseased CEC (D-CEC) showed a decrease in the CPR chymotrypsin-like, trypsin-like and caspase-like activities, while healthy CEC (H-CEC) and CAOMECS showed higher activities. Quantitative analysis of IPR inducible subunit (B5i, B2i, and B1i) were performed and compared to CPR subunit (B5, B2, and B1) levels. Results showed that ratios B5i/B5, B2i/B2 and B1i/B1 were higher in D-CEC, indicating that D-CEC had approximately a two-fold increase in the amount of IPR compared to CAOMECS and H-CEC. Histological analysis demonstrated that CAOMECS-grafted corneas had a re-epithelialized surface, positive staining for CPR subunits, and weak staining for IPR subunits. In addition, digital quantitative measurement of fluorescent intensity showed that the CPR B5 subunit was significantly more expressed in CAOMECS-grafted corneas compared to non-grafted corneas with LSCD.Conclusion
CAOMECS grafting successfully replaced the D-CEC with oral mucosal epithelial cells with higher levels of CPR. The increase in constitutive proteasome expression is possibly responsible for the recovery and improvement in CAOMECS-grafted corneas. 相似文献20.
Shao Dan Zhang Jing Na He Tong Tong Niu Shan Shan Liu Chiu Yeung Chan Chun Yang Ren Chi Liu Chi Pui Pang Yang Qu Ruo Xi Li Hai Lin Wang 《The ocular surface》2018,16(1):70-76