UF-1000i尿有形成分分析仪出现异常结果的常见原因及解决办法

SysmexUF-1000i全自动尿有形成分分析仪使用了荧光染色和流式细胞术的原理,通过红色半导体激光束照射染色后在鞘流贯流分析池中形成的鞘流样本,并通过对各粒子产生的侧向散射光、前向散射光以及荧光的信号转换成的光电信号进行分析,从而对尿液有形成分进行识别。该仪器操作简便快速（100测试／小时）、无需离心、定量检测、稳定、准确、重复性好等特点,能筛选出需做显微镜检查的标本、能提供显微镜检查不能获得的细胞信息。     

AVE-766尿液有形成分分析仪与UF-1000i尿液有形成分分析仪检测结果的比较

目的对不同方法检测尿液红细胞(RBC)、白细胞(WBC)、管型以及异形RBC的结果进行比较。方法分别应用相差显微镜人工镜检、AVE-766尿液有形成分分析仪(简称AVE-766)和UF-1000i尿液有形成分分析仪(简称UF-1000i)检测276例患者尿液中RBC、WBC、管型和异形RBC数量,并计算和比较阳性率、敏感性和特异性等实验诊断指标。结果人工镜检、AVE-766和UF-1000i检出RBC的阳性率分别为35.51%、38.77%和39.86%,WBC的阳性率分别为15.22%、20.29%和20.65%,管型阳性率分别为15.22%、17.75%和29.71%。以人工镜检为金标准,AVE-766和UF-1000i检测RBC的敏感性分别为91.84%和89.80%,检测WBC的敏感性分别为90.48%和88.10%,检测管型的敏感性分别为83.33%和85.71%。AVE-766检测RBC、WBC和管型的特异性分别为90.45%、92.31%和94.02%,UF-1000i检测RBC、WBC和管型的特异性分别为87.64%、91.45%和80.34%。与人工镜检相比,AVE-766检测非均一性、混合性和均一性血尿的符合率分别为82.61%、81.82%和85.71%,UF-1000i检测非均一性、混合性和均一性血尿的符合率分别为81.16%、72.73%和71.40%。统计学分析显示,除UF-1000i检测管型及判断血尿均一性的结果与人工镜检一致性较差外,AVE-766检测RBC、WBC、管型、血尿均一性及UF-1000i检测RBC、WBC的一致性都较好。结论与人工镜检相比,AVE-766和UF-1000i进行尿液有形成分检测的准确率都较高,但仍需要人工审核镜下实景图或联合人工镜检以提高检验质量。     

UF-1000i尿有形成分分析仪检测尿管型的评价

目的:以显微镜检测为金标准,评价UF-1000i尿有形成分分析仪检测尿管型的结果.方法:收集患者的尿标本364份,用UF-1000i全自动尿有形成分分析仪与显微镜分别检测尿液中尿管型,然后将两者的检测结果进行比较.结果:UF-1000i全自动尿有形成分分析仪检测尿管型的敏感性95.4%,特异性74.1%,阳性预测值61.2%,阴性预测值97.4%,假阳性率为18.1%,假阴性率为1.4%,准确率80.5%.结论:多种因素可以影响UF-1000i全自动尿有形成分分析仪对尿液管型的检测结果.因此,应用UF-1000i全自动尿有形成分分析仪检测尿液管型只能作为一种初步筛查方法.当仪器提示管型阳性时,应进行显微镜复检,提高检测结果的准确度.     

UF-1000i尿沉渣分析仪与显微镜联合检测尿液有形成分结果比较

目的对UF-1000i全自动尿沉渣分析仪联合显微镜镜检测定尿中白细胞、红细胞、结晶、类酵母菌及黏液丝的结果进行比较。方法收集2000例尿液样本,分别进行UF-1000i全自动尿沉渣分析仪与显微镜检测。结果UF-1000i全自动尿沉渣分析仪与显微镜联合检测尿中白、红细胞,结晶,类酵母,黏液丝的阳性率差异无统计学意义(P0.05)。结论UF-1000i全自动尿沉渣分析仪与显微镜镜检联合应用可以提高检测精确度。     

浅析UF-1000i全自动尿液有形成分分析仪管型报告与镜检结果的差异

目的 探讨UF-1000i全自动尿液有形成分分析仪和显微镜镜检两种方法检测尿中管型结果差异的原因.方法 采用Sysmex UF-1000i全自动尿液有形成分分析仪对200份新鲜晨尿标本进行尿沉渣分析,并同时对每一标本进行显微镜镜检,采用t、χ2检验对两种方法检测结果进行统计学分析.结果 200份标本中UF-1000i全自动尿液有形成分分析仪检出阳性93例,显微镜镜检检出阳性62例,假阳性率为28.26%,二者检测结果比较,差异有统计学意义(P<0.05).两种方法联合可将检测灵敏度提高至96.77%(P<0.05).结论 UF-1000i全自动尿液有形成分分析仪检测尿液管型时干扰因素较多,存在一定的假阳性结果.临床工作中应将两种方法结合起来,以提高检出率和准确性.     

UF-1000i尿有形成分分析仪性能评估及参考区间的建立

尿液常规分析是临床最常用的检验诊断项目,尿有形成分检查是尿液常规分析的重要部分。从1991~2009年的近20年间,国内外均在不断加强尿有形成分方法学的标准化、规范化建设［1］,目前国内主要以显微镜的形态学检查和流式细胞计数法（FCM）［2］。FCM以UF-1000i全自动尿有形成分分析仪为代表,但由于尿液标本的复杂性和现有技术局限性,目前只能属于筛选工具［1］,无法完全替代显微镜检查。为了解该仪器的性能状态,根据CNAS和CAP认可要求和CLSI相关标准,作者对该仪器的主要性能参数进行评价。报告如下。     

UF-1000i尿有形成分分析仪对尿路感染早期诊断的价值

目的 探讨UF-1000i尿有形成分分析仪对尿路感染的诊断价值.方法 采用UF-1000i尿有形成分分析仪检测110份尿液标本,记录其中的白细胞、细菌计数及尿路感染(urinary tract infection,UTI)信息,同时将尿标本进行细菌培养及鉴定,并将结果与UF-1000i尿有形成分分析仪结果相比较判断其符合情况.结果 通过对104份尿液标本的比较分析,UF-1000i尿有形成分分析仪的阳性率为24.04%(25/104),细菌培养阳性率为21.15%(22/104),两种检测方法间差异无统计学意义(χ2=0.09,P>0.05).将上述110份标本以UF-1000i分析仪检测细菌数,将尿液细菌培养结果作为金标准,与UF-1000i分析仪检测细菌定量值,绘制ROC曲线,确定UF-1000i分析仪测定细菌定量临界值为≥4 920.1/μl.此时,灵敏度为81.81%,特异度为91.46%.结论 UF-1000i尿有形成分分析仪的UTI信息对诊断尿路感染具有重要价值;细菌沉渣定量为≥4 920.1/μl,时,可为临床诊断泌尿道感染提供有力依据.     

UF-1000i尿有形成分分析仪在尿路感染筛查中的应用评价

目的评价UF-1000i尿流式有形成分分析仪在尿路感染（UTI）筛查中的应用价值。方法应用UF—1000i尿流式有形成分分析仪检测148例疑似uTI患者中段尿标本中的细菌（BACT）数和白细胞（WBC）数,以定量尿细菌培养作为诊断UTI金标准,应用受试者工作特征（ROC）曲线确立阳性判断标准,评价UF-1000i尿流式有形成分分析仪诊断UTI的敏感性、特异性、阳性预测值、阴性预测值和准确性。结果UF—1000i尿有形成分分析仪BACT和WBC诊断UTI的cut—off值分别为325个／汕和48个／μL,其诊断UTI的敏感性、特异性、阳性预测值、阴性预测值、准确性分别是：78．3％、92．2％、81．8％、90．4％、87．8％和73．9％、81．4％、64．2％、87．4％、79．1％。UF—1000i联合诊断uTI的敏感性、特异性、阳性预测值、阴性预测值和准确性分别95．7％、70．6％、59．5％、97．3％、78．4％。结论UF．1000i尿有形成分分析仪可作为UTI的快速筛查工具。     

UF-1000i流式尿有形成分分析仪、尿干化学分析仪及尿沉渣镜检联合检测尿液成分的临床应用价值

目的探讨联合应用UF-1000i尿有形成分分析仪(以下简称UF-1000i)、尿干化学分析仪(以下简称干化学)及尿沉渣显微镜检测(以下简称镜检)尿液成分的临床价值。方法随机收集兴安盟人民医院门诊、住院患者新鲜尿标本1 600例,分别用UF-1000i、干化学及镜检检测,并进行统计分析。结果 UF-1000i与干化学检测结果比较差异有统计学意义(P<0.05);以镜检为标准,UF-1000i检测红细胞阳性率11.6%,假阳性率4.88%、假阴性率5.38%;检测白细胞阳性率13.1%,假阳性率2.44%、假阴性率2.19%,检测管型阳性率7.75%,假阳性率6.25%、无假阴性;检测上皮细胞阳性率10.88%,假阳性率1.69%、假阴性率4.56%。UF-1000i与干化学联合检测与镜检比较:678例阳性标本中,UF-1000i红细胞阳性5例,镜检为非晶型盐类2例、真菌3例;干化学36例蛋白阳性、8例红细胞阳性,镜检阴性;922例阴性标本中,6例WBC镜检阳性(2~4/HP)。结论无论是UF-1000i、干化学,还是UF-1000i与干化学联合检测均不能代替镜检,UF-1000i与干化学联合检测可起筛查作用,只...     

UF-1000i尿液有形成分分析仪的性能评价

目的 评价Sysmex UF-1000i尿液有形成分分析仪的性能.方法 检测各项主要参数的精密度、准确度、线性范围及携带污染率. 结果批内及批间精密度、准确度、线性及携带污染率等均在仪器要求范围内.结论 Sysmex UF-1000i尿液有形成分分析仪各项性能均符合仪器要求范围,适用于大中型医院.     

Sysmex UF-1000i尿有形成分分析仪及AVE764B尿沉渣分析仪与手工镜检结果比较

目的以传统的手工镜检作为标准方法,对Sysmex UF-1000i尿有形成分分析仪(简称UF-1000i)、AVE764B尿沉渣分析仪(简称AVE764B)的定量分析检测结果进行比对,分析2种不同检测原理的检测系统的优缺点。方法收集200例泌尿科、肾内科等患者新鲜尿液(1 h内)标本进行分析,对2个分析系统做评估。结果手工镜检与UF-1000i比较,红细胞:Y=1.173X-3.277,r=0.975,F=3 771.028,P=0.000;白细胞:Y=0.658X+35.842,r=0.973,F=4 759.543,P=0.000;上皮细胞:Y=0.604X+11.252,r=0.973,F=1 378.888,P=0.000。手工镜检与AVE764B比较,红细胞:Y=1.27X-41.857,r=0.973,F=3 585.945,P=0.000。白细胞:Y=0.741X-9.402,r=0.990,F=10 210.826,P=0.000;上皮细胞:Y=0.486X-9.852,r=0.959,F=2 271.304,P=0.000。结论 2个分析系统结果基本与手工镜检相符,相关性均较好,尿沉渣分析仪对尿有形成分有较好的识别分析能力,但实际工作中对部分特殊标本仍需人工镜检识别确定。     

UF-1000i尿液有形成分分析仪、干化学法和镜检法对尿液分析的比较

目的探讨Sysmex UF-1000i全自动尿液有形成分分析仪（简称UF-1000i）对尿液中红细胞（RBC）、白细胞（WBC）和管型（CAST）检测的敏感性。方法对1 054例患者的尿液分别用UF-1000i、URISys-2400全自动干化学分析仪（干化学法）及Diasys R/S2005定量分析工作站（镜检法）3种方法进行分析,并比较3种方法对RBC、WBC、CAST的检测敏感性。结果 UF-1000i检测RBC、WBC、CAST的阳性率分别为20.6%、20.3%、6.7%;干化学法检测出RBC、WBC的阳性率分别为24.5%、17.9%;镜检法检出RBC、WBC、CAST的阳性率分别为16.9%、20.5%、2.2%。3种方法RBC、CAST的检出率差异有统计学意义（P〈0.01）,WBC的检出率差异无统计学意义（P〉0.05）。结论 UF-1000i不能完全取代尿沉渣镜检,建议3种方法联合应用以减少检验误差,提高尿液分析质量。     

UF-1000i全自动尿有形成分分析仪对尿路感染的诊断价值

Objective To evaluate the application value of UF-1000i automated urine formed elements analyzer in the diagnosis of urinary tract infection. Methods 150 urine specimens were analyzed using the UF-1000i in parallel with detection of leukocyte, yeast-like fungus, and bacteria. These detection results were collected for evaluation of urinary tract infection and scatter grams were recorded. At the same time, these samples were cultured for bacterial identification, which results were compared with that of the UF-1000i. The clinical diagnose criteria of the UTI was performed as golden standard. As compare with results obtained with UF-1000i, the sensitivity and specificity of UF-1000i for diagnosis of urinary tract infection were evaluated, and the consistency were analyzed among scatter grams, bacterial culture and final diagnosis. Results The statistical results from 146 specimens showed that the positive rate of UF-1000i was 32. 9% (48/146), the positive rate of urine culture is 28. 8% (42/146). There was no significant statistical difference found (χ2 = 1.79 ,P = 0. 18 )and Kappa test showed a considerable consistency (K = 0. 775 6). The UF-1000i detection results showed the sensitivity 76. 0% ( 38/50 ), specificity 89. 6% ( 86/96 ), positive predictive value 79. 2% ( 38/48 ) and negative predictive value 87. 8% ( 86/98 ), respectively. The distribution of coccus and bacilli obtained from the UF-1000i testing was basically in accordance with the results of bacterial culture. Conclusion The "UTI-information" of UF-1000i is very important for the diagnosis of urinary tract infections.     

UF-1000i全自动尿有形成分分析仪对尿路感染的诊断价值

Objective To evaluate the application value of UF-1000i automated urine formed elements analyzer in the diagnosis of urinary tract infection. Methods 150 urine specimens were analyzed using the UF-1000i in parallel with detection of leukocyte, yeast-like fungus, and bacteria. These detection results were collected for evaluation of urinary tract infection and scatter grams were recorded. At the same time, these samples were cultured for bacterial identification, which results were compared with that of the UF-1000i. The clinical diagnose criteria of the UTI was performed as golden standard. As compare with results obtained with UF-1000i, the sensitivity and specificity of UF-1000i for diagnosis of urinary tract infection were evaluated, and the consistency were analyzed among scatter grams, bacterial culture and final diagnosis. Results The statistical results from 146 specimens showed that the positive rate of UF-1000i was 32. 9% (48/146), the positive rate of urine culture is 28. 8% (42/146). There was no significant statistical difference found (χ2 = 1.79 ,P = 0. 18 )and Kappa test showed a considerable consistency (K = 0. 775 6). The UF-1000i detection results showed the sensitivity 76. 0% ( 38/50 ), specificity 89. 6% ( 86/96 ), positive predictive value 79. 2% ( 38/48 ) and negative predictive value 87. 8% ( 86/98 ), respectively. The distribution of coccus and bacilli obtained from the UF-1000i testing was basically in accordance with the results of bacterial culture. Conclusion The "UTI-information" of UF-1000i is very important for the diagnosis of urinary tract infections.     

UF-1000i全自动尿有形成分分析仪对尿路感染的诊断价值

Objective To evaluate the application value of UF-1000i automated urine formed elements analyzer in the diagnosis of urinary tract infection. Methods 150 urine specimens were analyzed using the UF-1000i in parallel with detection of leukocyte, yeast-like fungus, and bacteria. These detection results were collected for evaluation of urinary tract infection and scatter grams were recorded. At the same time, these samples were cultured for bacterial identification, which results were compared with that of the UF-1000i. The clinical diagnose criteria of the UTI was performed as golden standard. As compare with results obtained with UF-1000i, the sensitivity and specificity of UF-1000i for diagnosis of urinary tract infection were evaluated, and the consistency were analyzed among scatter grams, bacterial culture and final diagnosis. Results The statistical results from 146 specimens showed that the positive rate of UF-1000i was 32. 9% (48/146), the positive rate of urine culture is 28. 8% (42/146). There was no significant statistical difference found (χ2 = 1.79 ,P = 0. 18 )and Kappa test showed a considerable consistency (K = 0. 775 6). The UF-1000i detection results showed the sensitivity 76. 0% ( 38/50 ), specificity 89. 6% ( 86/96 ), positive predictive value 79. 2% ( 38/48 ) and negative predictive value 87. 8% ( 86/98 ), respectively. The distribution of coccus and bacilli obtained from the UF-1000i testing was basically in accordance with the results of bacterial culture. Conclusion The "UTI-information" of UF-1000i is very important for the diagnosis of urinary tract infections.     

UF-1000i全自动尿有形成分分析仪对尿路感染的诊断价值

Objective To evaluate the application value of UF-1000i automated urine formed elements analyzer in the diagnosis of urinary tract infection. Methods 150 urine specimens were analyzed using the UF-1000i in parallel with detection of leukocyte, yeast-like fungus, and bacteria. These detection results were collected for evaluation of urinary tract infection and scatter grams were recorded. At the same time, these samples were cultured for bacterial identification, which results were compared with that of the UF-1000i. The clinical diagnose criteria of the UTI was performed as golden standard. As compare with results obtained with UF-1000i, the sensitivity and specificity of UF-1000i for diagnosis of urinary tract infection were evaluated, and the consistency were analyzed among scatter grams, bacterial culture and final diagnosis. Results The statistical results from 146 specimens showed that the positive rate of UF-1000i was 32. 9% (48/146), the positive rate of urine culture is 28. 8% (42/146). There was no significant statistical difference found (χ2 = 1.79 ,P = 0. 18 )and Kappa test showed a considerable consistency (K = 0. 775 6). The UF-1000i detection results showed the sensitivity 76. 0% ( 38/50 ), specificity 89. 6% ( 86/96 ), positive predictive value 79. 2% ( 38/48 ) and negative predictive value 87. 8% ( 86/98 ), respectively. The distribution of coccus and bacilli obtained from the UF-1000i testing was basically in accordance with the results of bacterial culture. Conclusion The "UTI-information" of UF-1000i is very important for the diagnosis of urinary tract infections.     

UF-1000i全自动尿有形成分分析仪对尿路感染的诊断价值

Objective To evaluate the application value of UF-1000i automated urine formed elements analyzer in the diagnosis of urinary tract infection. Methods 150 urine specimens were analyzed using the UF-1000i in parallel with detection of leukocyte, yeast-like fungus, and bacteria. These detection results were collected for evaluation of urinary tract infection and scatter grams were recorded. At the same time, these samples were cultured for bacterial identification, which results were compared with that of the UF-1000i. The clinical diagnose criteria of the UTI was performed as golden standard. As compare with results obtained with UF-1000i, the sensitivity and specificity of UF-1000i for diagnosis of urinary tract infection were evaluated, and the consistency were analyzed among scatter grams, bacterial culture and final diagnosis. Results The statistical results from 146 specimens showed that the positive rate of UF-1000i was 32. 9% (48/146), the positive rate of urine culture is 28. 8% (42/146). There was no significant statistical difference found (χ2 = 1.79 ,P = 0. 18 )and Kappa test showed a considerable consistency (K = 0. 775 6). The UF-1000i detection results showed the sensitivity 76. 0% ( 38/50 ), specificity 89. 6% ( 86/96 ), positive predictive value 79. 2% ( 38/48 ) and negative predictive value 87. 8% ( 86/98 ), respectively. The distribution of coccus and bacilli obtained from the UF-1000i testing was basically in accordance with the results of bacterial culture. Conclusion The "UTI-information" of UF-1000i is very important for the diagnosis of urinary tract infections.     

UF-1000i全自动尿有形成分分析仪对尿路感染的诊断价值

Objective To evaluate the application value of UF-1000i automated urine formed elements analyzer in the diagnosis of urinary tract infection. Methods 150 urine specimens were analyzed using the UF-1000i in parallel with detection of leukocyte, yeast-like fungus, and bacteria. These detection results were collected for evaluation of urinary tract infection and scatter grams were recorded. At the same time, these samples were cultured for bacterial identification, which results were compared with that of the UF-1000i. The clinical diagnose criteria of the UTI was performed as golden standard. As compare with results obtained with UF-1000i, the sensitivity and specificity of UF-1000i for diagnosis of urinary tract infection were evaluated, and the consistency were analyzed among scatter grams, bacterial culture and final diagnosis. Results The statistical results from 146 specimens showed that the positive rate of UF-1000i was 32. 9% (48/146), the positive rate of urine culture is 28. 8% (42/146). There was no significant statistical difference found (χ2 = 1.79 ,P = 0. 18 )and Kappa test showed a considerable consistency (K = 0. 775 6). The UF-1000i detection results showed the sensitivity 76. 0% ( 38/50 ), specificity 89. 6% ( 86/96 ), positive predictive value 79. 2% ( 38/48 ) and negative predictive value 87. 8% ( 86/98 ), respectively. The distribution of coccus and bacilli obtained from the UF-1000i testing was basically in accordance with the results of bacterial culture. Conclusion The "UTI-information" of UF-1000i is very important for the diagnosis of urinary tract infections.