共查询到20条相似文献,搜索用时 11 毫秒
1.
Antibody-mediated immune function in adult and recently fledged (30 to 33 d old) American kestrels (Falco sparverius) was examined in birds exposed directly, or only in ovo, to polychlorinated biphenyls (PCBs). In 1998, 9 mature male and 9 female kestrels were fed PCBs, whereas 9 females and 10 males served as controls. A mixture of Aroclors 1248:1254:1260 suspended in safflower oil was injected into the kestrels' food items, while in control diets only the same volume of oil was added. The dosage of PCBs was approximately 7 mg/kg kestrel/d, beginning in March 1998 and continuing for 120 d. In 1998, the antibody-mediated immune response was stimulated by immunization and booster vaccinations of the kestrels using a nonpathogenic antigen, dinitrophenol-keyhole limpet hemocyanin (DNP-KLH). In 1999, offspring from three treatment groups based upon maternal exposure to PCBs were similarly tested for their antibody response. None of these mothers was vaccinated with DNP-KLH the previous year. The maternal groups were: (1) exposed to PCBs in 1998 for 120 d, (2) exposed in ovo in 1998 (i.e., mothers were produced by PCB-exposed parents), or (3) unexposed to PCBs. Serum antibody levels were determined using an enzyme-linked immunosorbent assay (ELISA). In 1998, PCB-exposed adult females had a significantly higher antibody response than did controls, whereas adult males exposed to PCBs had significantly suppressed antibody production. For the nestlings produced in 1999, maternal treatment significantly affected antibody response. Generally, the antibody response in the nestlings was much lower than that seen in adult kestrels. Yet both male and female offspring from mothers that had been fed PCBs the previous year had significantly higher postbooster anti-DNP-KLH titers than control and in ovo-exposed maternal groups, thus mimicking the response seen in the adult females the previous year. These sex-specific responses in PCB-exposed birds provide further evidence of the endocrine-disrupting behavior of PCBs. Both suppression and stimulation of the antibody response are undesirable because this indicates that the immune system is not able to respond normally to challenges by infectious or other disease-causing agents. 相似文献
2.
Fernie K Bortolotti G Smits J 《Journal of toxicology and environmental health. Part A》2003,66(22):2089-2103
This study found abnormalities in multiple reproductive stages in captive American kestrels (Falco sparverius) when exposed to polychlorinated biphenyls (PCBs) through dietary and in ovo exposure. American kestrels laid eggs with environmentally relevant total PCB levels (34.1 micrograms/g whole egg wet weight) when consuming PCB-spiked (Aroclor 1248:1254:1260) food (5-7 micrograms/g body weight per day) for 100 d only in 1998. In 1999, the same adults laid eggs with estimated total PCBs of 23 micrograms/g. Effects of maternal (only female exposed) and paternal (only male exposed) in ovo PCB exposure were investigated. Maternal F1 eggs contained predicted total PCB concentrations of 0.34 microgram/g. Specific abnormalities occurred more frequently during dietary F0 exposure, particularly aggressive courtship interactions, clutch abandonment, occurrences of cracked eggs, and developmental effects. Multiple developmental effects were more pronounced during than after dietary PCB exposure of adults, and although these effects were limited, nevertheless they occurred in the F1 maternal and F1 paternal pairs. However, the incidence of multiple deformities throughout the breeding season increased dramatically from 1998 (13%) to 1999 (56%) in F0 PCB-exposed pairs. Developmental abnormalities were unlikely to be attributed to the extrinsic factors of disease, genetics, or nutritional (vitamin D3) deficiencies, but rather to adverse changes in parental behavior and intrinsic factors involving altered genetic material and PCB exposure. Readily cleared PCB congeners may induce specific types of behavioral and developmental abnormalities, but persistent congeners and metabolites are likely producing (1) odd laying patterns, (2) odd laying patterns, (2) developmental effects including embryonic underdevelopment and edema, and (3) increased incidences of multiple deformities within a clutch. 相似文献
3.
The effects of exposure to polychlorinated biphenyls (PCBs) on dental caries of first permanent molars in children in the Faroe Islands, where the population is exposed to PCBs in their traditional diet, were evaluated. This study was part of a cohort study aimed at determining developmental risks resulting from the consumption of contaminated seafood. One hundred and fourteen children from a birth cohort of 182 mother-child pairs, aged 9-10 years, were examined. Caries scoring was performed according to WHO criteria; cavitated and noncavitated lesions were included. Exposure assessment was based on the PCB concentrations in maternal milk samples. Data from the project data set provided information on various confounding factors. Mean maternal milk PCB concentration was 2205 (range 70-11,150)ng/g lipids. The number of carious surfaces of first permanent molars correlated with milk PCB concentration (r=0.26, p=0.01). In multivariate linear regression analysis, PCB exposure was significantly related to caries susceptibility. The findings support an association between children's PCB exposure and caries prevalence. 相似文献
4.
Serum hormones in boys prenatally exposed to polychlorinated biphenyls and dibenzofurans 总被引:4,自引:0,他引:4
Hsu PC Lai TJ Guo NW Lambert GH Guo YL 《Journal of toxicology and environmental health. Part A》2005,68(17-18):1447-1456
Polychlorinated biphenyls (PCBs) and dibenzofurans (PCDFs) are persistent environmental pollutants shown to adversely interact with several functions of the endocrine system. In 1978-1979, over 2000 Taiwanese people ingested rice oil accidentally contaminated with PCBs and PCDFs. This is one of the major toxic exposure episodes that occurred globally and was later called Yucheng (oil disease in Chinese). The children born to exposed Yucheng women were therefore exposed in utero to high doses of PCBs/PCDFs. In 1995, 60 Yucheng and 61 control boys participated in physical examination, and serum hormones were measured by radioimmunoassay (RIA). Age, body weight, body height, Tanner status, testicular size, serum luteinizing hormone (LH), prolactin (PRL), thyroxine (T4), triiodothyronine (T3), and thyroid-stimulating hormone (TSH) levels were not statistically different between Yucheng and control boys in the subgroups of before and at the age of puberty. However, the serum estradiol (E2) levels were significant higher in Yucheng boys at the age of puberty. Yucheng and control boys were further divided into two subgroups, those before (age <13 yr) and those at the age of puberty (age > or = 13 yr). There was a decrease of serum testosterone (TT) levels and increase of serum follicle-stimulating hormone (FSH) levels in Yucheng boys at the age of puberty as compared with controls. There was a significant decrease of the square root of TT/E2 and TT/FSH; however, the square root of E2/FSH was increased in Yucheng boys at the age of puberty as compared with controls. Data indicated that prenatal exposure to PCBs and PCDFs may have implications for boys' sex hormone homeostasis at puberty. Further studies are needed to identify the congeners of PCBs/PCDFs responsible for disruption of the endocrine system, as well as the mechanisms of such disruption. 相似文献
5.
The aim of this paper has been to assess the exposure of the Slovak Republic population to polychlorinated biphenyls from food within 1994-2004. A total of 61167 samples of food of animal origin, including fats, were analyzed for the content of six PCB congeners (the sum of: PCB-28, PCB-52, PCB-101, PCB-138, PCB-153 and PCB-180); the commodities were divided into 48 basic groups. The exposure dose values based on actual and model consumption were calculated for an average inhabitant as well as for children of various age groups, and compared with the Tolerable Daily Intake value (TDI; 0.4 microg kg(-1) body weight per day). Knowledge and experience from a variety of projects running since 1991, as well as the database of food contaminants of the Centre for Evaluation of Contaminant Occurrence established at the Food Research Institute were utilized. The results of the evaluation suggested, that during the period of observation, the exposure of the Slovak Republic population to PCBs was relatively low. Taking actual consumption and mean findings as the basis, the daily exposure doses of PCBs ranged between 3.1% and 6.5% TDI, the corresponding figures for median value and the 95th percentile being between 1.4% and 4.0%, and between 7.5% and 20.0%, respectively. 相似文献
6.
Philip J Bushnell Virginia C Moser Robert C MacPhail Wendy M Oshiro Ethel C Derr-Yellin Pamela M Phillips Prasada Rao S Kodavanti 《Toxicological sciences》2002,68(1):109-120
Because of behavioral deficits associated with gestational exposure to PCBs in children, we sought to quantify neurobehavioral effects of perinatal exposure to Aroclor 1254(R) (A1254), a commercial mixture of PCBs, in rats. Pregnant Long-Evans rats were fed A1254 at doses of 0, 1.0, or 6.0 mg/kg/day throughout gestation and nursing. The growth and behavior of their male and female offspring were assessed both during development and as adults, using a variety of behavioral tests that included a neurobehavioral screening battery (functional observational battery [FOB] and automated tests of locomotor activity), habituation of motor activity, acquisition of a visual discrimination, and performance of a visual signal-detection task. During the suckling period, A1254 at 6 mg/kg reduced survival and body weight gain of offspring of both sexes; however, locomotor activity was unaffected, and only small and transient changes in other measures were evident. In adulthood, perinatal exposure to A1254 did not affect habituation of locomotor activity, acquisition of the visual discrimination, or sustained attention. Rats performing the signal-detection task were challenged with cocaine (0, 1.25, 2.5, 5.0 mg/kg) and haloperidol (0, 0.003, 0.010, 0.030 mg/kg) to probe the integrity of dopaminergic systems in the central nervous system (CNS). A1254 did not alter the impairment of attention caused by haloperidol. Cocaine reduced false alarms more in controls than in rats exposed to A1254, but the effect was not clearly related to the dose of A1254. Perinatal exposure to this commercial PCB mixture had very little effect on these tests of behavior during development and in adulthood. 相似文献
7.
Reproductive hormone profile and pubertal development in 14-year-old boys prenatally exposed to polychlorinated biphenyls 总被引:1,自引:0,他引:1
P Grandjean C Grønlund IM Kjær TK Jensen N Sørensen AM Andersson A Juul NE Skakkebæk E Budtz-Jørgensen P Weihe 《Reproductive toxicology (Elmsford, N.Y.)》2012,34(4):498-503
Because polychlorinated biphenyls (PCBs) are thought to cause endocrine disruption, we examined 438 adolescent boys from a birth cohort in the Faroe Islands, where PCB exposures are elevated. We measured PCBs and p,p'-dichlorodiphenyldichloroethylene (DDE) in cord blood and in serum from clinical examination at age 14. Higher prenatal PCB exposure was associated with lower serum concentrations of both luteinizing hormone (LH) and testosterone. In addition, sex hormone binding globulin (SHBG) was positively associated with both prenatal and concurrent PCB exposures. The PCB-SHBG association was robust to covariate adjustment. In a structural equation model, a doubling in prenatal PCB exposure was associated with a decrease in LH of 6% (p=0.03). Prenatal exposure to PCB and DDE showed weak, non-significant inverse associations with testicular size and Tanner stage. DDE was highly correlated with PCB and showed slightly weaker associations with the hormone profile. These findings suggest that delayed puberty with low serum-LH concentrations associated with developmental exposure to non dioxin-like PCBs may be due to a central hypothalamo-pituitary mechanism. 相似文献
8.
The results of the present study strongly suggest that the hypertriglyceridaemia in chicks treated with Aroclor 1254 is a result of reduction in VLDL clearance rather than an increase in rate of VLDL secretion. The decrease in post-heparin LPL activity responsible for reduced VLDL clearance may be mediated by cytokines produced by chronic activation of the immune system. 相似文献
9.
Fernie KJ Laird Shutt J Ritchie IJ Letcher RJ Drouillard K Bird DM 《Journal of toxicology and environmental health. Part A》2006,69(16):1541-1554
Polybrominated diphenyl ether (PBDEs) concentrations are increasing exponentially in biota. We studied the growth of American kestrel (Falco sparverius) nestlings exposed in ovo and during development to environmentally relevant PBDE congeners and concentrations. Eggs within each clutch, divided between groups by laying sequence, were injected into the air cell at 19 days of incubation with safflower oil or penta-BDE congeners BDE-47, -99, -100, and -153 dissolved in safflower oil (18.7 microg total sigmaPBDEs/egg), approximating current levels in Great Lakes herring gulls. The measured proportions of BDE congeners found in the dosing oil were 56.4% of BDE-47; 27.2% of BDE-99; 24.8% of BDE-100; and 0.6% of BDE-153. For 29 days, nestlings were orally gavaged daily with the same sigmaPBDE mixture (15.6 +/- 0.3 ng/g body weight/day). Relative congener abundances in the dosing mixture compared to the carcasses suggest biotransformation of BDE-47; BDE-183 was also detected. PBDE exposure did not affect hatching or fledging success. PBDE-exposed nestlings were larger (weight, bones, feathers) as they gained weight more quickly and ate more food, the latter in association with their SigmaPBDE body burdens. BDE-100 was most influential on nestling growth, being positively associated with size, weight gain, and food consumption. Increasing concentrations of BDE-183 and -153 were related to longer bones, and BDE-99 to longer feathers. The larger size of the PBDE-exposed birds may be detrimental to their bone structure and have excessive energetic costs. The repeated relationships with BDE-100 and growth may be important for wild Falconidae, since this is the predominant penta-BDE congener in these raptors. 相似文献
10.
V A Tutel'ian L V Lashneva G K Sorokovaia A V Khan Z M Gadzhieva 《Farmakologiia i toksikologiia》1987,50(2):111-113
Contents of cytochrome P-450 and b5, rates of oxidation of aniline, amidopyrine and dimethylaniline as well as activities of NADP-H- and ascorbate-dependent systems of lipid peroxidation (LPO) in rat liver microsomes five months after single administration of the mixture of polychlorinated diphenyls (PCD) significantly exceeded the control level. Starvation of the animals for 120 hours led to an additional increase of cytochrome P-450 content and LPO activation. The rat liver monooxygenase system retained the ability to respond to the inducing action of the mixture of PCD (500 mg/kg) during starvation. 相似文献
11.
Induction of cytochrome P450 isoforms, specifically CYP1A1, and their catalytic activities are potential biomarkers of environmental contamination by polychlorinated biphenyls (PCBs). In this study, dogs were exposed to 25 ppm or 5 ppm Aroclor 1248 (PCB mixture) daily in their diet for 10 or 20 weeks, respectively. Relative to controls, hepatic microsomes from dogs dosed with PCBs had higher levels of CYP1A1 detected in immunoblots and higher levels of EROD activity, but low levels of induction for CYP2B and PROD activity. Concentrations of 96 PCB congeners in serum and liver were evaluated using capillary chromatography. Results showed that all dogs exposed to PCB mixtures had higher levels of PCB in serum and liver. Dogs preferentially sequestered highly chlorinated PCB congeners in liver relative to serum. With these experiments, we demonstrated that EROD activity was a potentially sensitive marker of PCB exposure at 5 and 25 ppm. Furthermore, CYP1A1 and EROD activity were maximally induced in dogs consuming dietary concentrations only 2.5 times the maximal permissible level for human food (FDA). The value of CYP1A1 induction as a biomarker of PCB exposure was tenuous because neither CYP1A1 levels nor EROD activity correlated with total PCB body burden. However, a small subset of congeners were identified in liver that may strongly influence EROD and PROD induction. Finally, two dogs in the 25 ppm dose group were fasted for 48 h. After 24 h of fasting, several new congeners appeared in the serum and remained in the serum for the remainder of the fast. The fast caused a 293% increase in PCB concentration in serum. This increase has strong implications regarding mobilization of toxic PCBs in wildlife during fasting (e.g., migration, hibernation). 相似文献
12.
Baldridge MG Stahl RL Gerstenberger SL Tripoli V Hutz RJ 《Reproductive toxicology (Elmsford, N.Y.)》2003,17(5):567-573
Polychlorinated biphenyls (PCBs) are ubiquitous man-made toxicants capable of endocrine disruption. Studies in several species have shown that exposure to PCBs and their hydroxylated metabolites reduces fecundity and decreases circulating concentrations of thyroid hormones, causing serious reproductive and developmental defects. Thyroid hormones modulate both follicular development and steroidogenesis, and affect estrogen metabolism and the regulation of estrogen receptor. This study was designed (1). to determine whether exposure to a commercially prepared PCB mixture (Aroclor 1016) exerts detrimental effects on follicle maturation in the Long-Evans hooded rat; and (2). to determine whether the modulatory effects of Aroclor can be attenuated by levo-thyroxine sodium (T(4)) supplementation. Animals were treated on gestation days 7-13 with a single daily intraperitoneal injection (2.5 mg/kg per day) of Aroclor. Half of the Aroclor-treated dams were also given T(4) supplements (2.89 microg/kg per day) via drinking water. Female pups were sacrificed on postnatal days 24/25, and the ovaries were excised, fixed for histology and analyzed. The analysis included a count, measurement and classification of healthy and atretic preantral and antral follicles in the greatest cross-sectional area. The results indicated that treatment with Aroclor significantly reduced the number of preantral follicles <50000 microm(2) and the total number of antral follicles in the 50-100000 and >100000 microm(2) size classes. T(4) circumvented the Aroclor effect on the number of preantral follicles <50000 microm(2); however, a significant reduction in the antral follicle number persisted in the 50-100000 and >100000 microm(2) size classes. In addition, we observed a significant increase in atresia in the Aroclor-treated ovaries in the antral <50000 microm(2) size class, which was not present in ovaries exposed to both Aroclor and T(4). These data support the hypothesis that Aroclor reduces the number of preantral and antral follicles of certain size classes in rats exposed during the critical period of development, and that supplementation with T(4) can attenuate the effects of Aroclor on small, but not medium or large antral follicles. Atresia of small, antral follicles may constitute one of the underlying mechanisms by which folliculogenesis is modulated by Aroclor 1016. 相似文献
13.
A commercial polychlorinated biphenyl mixture (PCBs), Aroclor 1242, was administered to rats po by intubation in order to determine toxic manifestations of acute and subacute ingestion. In addition, the effect of PCBs on hepatic microsomal enzyme systems in rats was evaluated. The oral, 14-day LD50 was determined to be approximately 4.25 g/kg. Major toxic signs observed upon administration of high doses of PCBs included diarrhea, chromodacryorrhea, loss of body weight, unusual stance and gait, lack of response to pain stimuli, and terminal ataxia. Progressive dehydration and CNS depression appeared to be contributing factors in each fatality. Histopathologic alterations were evident only in the liver and kidneys, manifest as foci of sudanophilic vacuolation. Rats maintained on an oral dosage regimen of 100 mg/kg every other day for 3 weeks exhibited similar histopathologic changes, but no overt signs of toxicity. Serum GOT activities were elevated over controls in both the acute and subacute groups. A single ip injection (100 mg/kg) increased liver weight, total hepatic microsomal enzyme activity (measured as hydroxylation of acetanilide and N-demethylation of aminopyrine), and hepatic cytochrome P450 and b5 levels. Hepatic microsomal enzyme activity remained elevated 10 days after a single dose of PCBs, suggesting that PCBs may play an important role in altering biologic responses of mammals subjected to environmental chemical stress. 相似文献
14.
Polychlorinated biphenyls (PCBs) may form reactive electrophiles that can bind covalently to hemoglobin in vivo. Female C57/BL6 mice, pretreated with phenobarbital and P-naphthoflavone, were injected with either radiolabeled 4-chlorobiphenyl, 3,3',4.4'-tetrachlorobiphenyl, benzo[a]pyrene (B[a]P) or the corresponding non-labeled 4-chlorobiphenyl, 3.3',4.4'-tetrachlorobiphenyl, B[a]P or vehicle. Blood was collected at different time points. At 24 h a greater presence of 4-chlorobiphenyl and 3,3',4.4'-tetrachlorobiphenyl in the plasma, compared with the erythrocytes, was observed. For all three treatment groups the radioactivity in the hemolysate was found to be greater than the vehicle-treated group, except at 2 h after dosing, suggesting an association with hemoglobin. Covalent binding with globin was 10-fold greater for 4-chlorobiphenyl-treated animals compared with 3,3',4,4'-tetrachlorobiphenyl-treated animals. Liquid chromatography-mass spectral analysis of globin was used to identify adducts. Our preliminary data show an increase in mass corresponding to adducts of oxidized metabolites of PCBs. Detection of adducts of PCBs with hemoglobin could provide a valuable tool to evaluate acute exposure of a population. 相似文献
15.
Trnovec T Sovčíková E Hust'ák M Wimmerová S Kočan A Jurečková D Langer P Palkovičová L Drobná B 《Environmental toxicology and pharmacology》2008,25(2):183-187
The objective of this cross-sectional epidemiological study was to assess if long-term exposure to polychlorinated biphenyls (PCBs) is associated with hearing impairment. Four hundred and thirty-three children aged 8-9 years residing in an area polluted by PCBs in Eastern Slovakia were examined otoscopically, tympanometrically and by pure tone audiometry. PCB levels in their serum were determined by gas chromatography. Transient otoacoustic emissions (TEOAE) were measured in a subgroup of 161 children. The mean of the sum of PCB concentrations in serum was 528.2ng/g serum lipids (median 321ng/g serum lipids). Serum PCB concentrations were associated with an increase of hearing threshold at low frequencies and a negative correlation between serum PCBs and the amplitude of TEOAE response was observed in the uppermost tertile of children grouped with regard to serum PCBs, not related to thyroid hormone levels. It was concluded that long-term environmental exposure to PCBs is associated with subclinical but diagnosable hearing deficits. 相似文献
16.
17.
Love OP Shutt LJ Silfies JS Bortolotti GR Smits JE Bird DM 《Ecotoxicology (London, England)》2003,12(1-4):199-208
We experimentally examined the effects of dietary exposure to polychlorinated biphenyls (PCBs) on adrenocortical function in American kestrels (Falco sparverius). Nine captive male American kestrels previously exposed to a PCB mixture (Aroclor1248:1254:1260; 1:1:1) in their diet were subjected to a standardized capture, handling and restraint protocol designed to produce an increase in circulating corticosterone. A similar protocol has been applied to a wide range of avian species and was used here to evaluate the response of PCB-exposed and control kestrels to a defined physical stressor. Both baseline and stress-induced corticosterone levels were significantly lower in PCB-exposed birds when compared with control birds of the same age. PCB-exposed birds exhibited significantly lower corticosterone levels during the corticosterone response when compared with control birds, independent of body condition. Furthermore, baseline corticosterone concentrations exhibited a hormetic response characterized by an inverted U-shaped dose response in relation to total PCB liver burden. These results support several recent studies which report decreased levels of circulating corticosterone in PCB-exposed wild birds. The results presented here provide the first evidence that exposure to an environmentally relevant level of PCBs (approximately 10 mg/kg body weight) can impair the corticosterone stress response in kestrels, potentially increasing the susceptibility of birds to environmental stressors such as severe weather and predatory and human disturbance. 相似文献
18.
Miyuki Shimada Satomi Kameo Norio Sugawara Kozue Yaginuma-Sakurai Naoyuki Kurokawa Satomi Mizukami-Murata Kunihiko Nakai Hitoshi Iwahashi Hiroshi Satoh 《Archives of toxicology》2010,84(4):271-286
Methylmercury (MeHg) and polychlorinated biphenyls (PCBs) are environmentally persistent neurodevelopmental toxicants. The
primary source of human exposure is the consumption of contaminated fish, seafood and marine mammals. However, little is known
about the molecular mechanisms of MeHg and PCB toxicities and interactions between these contaminants. We investigated the
functional profiles of differently expressed genes in the brains of offspring mice perinatally exposed to MeHg and/or PCBs
to elucidate how these contaminants interact with each other. Pregnant mice (C57BL/6) were divided into four groups by exposure:
(1) vehicle control, (2) MeHg alone, (3) PCBs alone, (4) MeHg + PCBs. Gene expression analysis of the brains of offspring
mice was carried out with 4 × 44 K whole mouse genome’s microarrays (Agilent) on postnatal day 1. The gene expression pattern
of the MeHg exposure-group differed from that of the PCB-exposure group. The MeHg + PCB group expressed a larger number of
genes, most of which were not expressed in the MeHg group or PCB group. It was revealed that gene expression was greatly increased,
and the most altered genes were found with co-exposure. The genes were related to the functional categories of development,
inflammation, calcium ion homeostasis, signal transduction, the ubiquitin–proteasome pathway and detoxication. The ubiquitin–proteasome
system and detoxication categories might function for protection against the toxicity induced by co-exposure to MeHg and PCBs.
These results suggest that co-exposure does not simply exacerbate the toxicity of MeHg alone or PCB alone, but stimulates
a protection system. 相似文献
19.