纤维蛋白粘合剂与羟基磷灰石复合人工骨的实验研究

以具有良好生物相容性和生物降解性的纤堆蛋白(FS)粘合剂作为颗粒型羟基磷灰石人工骨(HA)的粘接成形剂，将HA—PS复合材料植入狗下颌实验性骨缺损中，采用组织学、定量组织学、扫描电镜和X线等方法评价其骨缺损修复能力。研究结果发现，HA—FS复合人工骨具有良好的组织相容性、骨引导性，能与新骨形成紧密结合，阻止HA颗粒散动及改善其操作性能，PS在植入后2周完全吸收。本研究表明，HA—PS复合人工骨作为骨修复材料，具有良好组织相容性和较理想的操作性能。     

煅石膏、骨形成蛋白及羟基磷灰石三元复合人工骨修复骨缺损的实验研究

以煅石膏(PLP）作为颗粒型羟基磷灰石(HA)人工骨粘接成形剂和骨形成蛋白(BMP)的载体，制成三元复合人工骨．分别用HA—bBMP、HA—PLP和单纯HA植入狗下颌骨实验性骨缺损中，采用组织学、定量组织学、免疫组织化学、X线摄片和扫描电镜观察的方法评价该复合人工骨的生物学性能。术后1，2，4，8和16周观察发现，HA—bBMP—PLP复合人工骨具有明显的骨诱导活性．PLP可充当BMP缓慢释放系统载体．增强BMP骨诱导活性，和作为颗粒型HA的粘接成形剂．使复合人工骨具有一定的可塑性和成形性，可达到准确的植入，植入后早期可有效防止HA颗粒移动。本研究证实．HA—bBMP—PLP三元复合人工骨不但可限制植入后HA颗粒的早期移动，更重要的是可以迅速增加新骨形成量．从形态和功能上大大提高了复合人工骨修复骨缺损的质量。     

胶原与羟基磷灰石复合人工骨的实验研究

以具有良好生物相容性和生物降解性的胶原为颗粒型羟基磷灰石人工骨的粘接成形剂，将ＨＡ－胶原复合材料植入狗下颌实验性骨缺损中，采用组织学、定量组织学和Ｘ线等方面法评价其骨缺损修复能力。研究结果发现ＨＡ－胶原复合人工骨具有良好的相容性、骨引导性，能与新骨形成紧密结合，抑制ＨＡ颗粒散动及改善其操作性能，胶原在植入后４周完全吸收。本研究表明，ＨＡ－胶原复合人工骨作为新的骨修复材料，人有良好的组织相容性和较理     

胶原/纳米磷酸三钙复合人工骨修复骨缺损的实验研究

目的 观察胶原（Collagen,简称Co)／纳米磷酸三钙（Nano-Tricalcium Phosphate,简称N－TCP）复合人工骨的生物相容性，Co/N-TCP植入骨缺损区促进骨创愈合的能力。方法 家兔24只，颅骨制成直径8.0mm圆形穿通型缺损，左侧植入Co/N-TCP复合人工骨作为实验组，右侧植入非纳米Co/TCP人工骨作为对照组；于2，4，8，12周后处死各组动物取材，行肉眼，组织学方法观察。结果 两组材料除早期（2周内）轻微炎性2细胞浸润外，其余各时间点均未见异物巨细胞反应，4－8周骨新生活跃，有骨岛形成，12周时可见骨性连接。结论 Co/N-TCP复合人工骨具有良好的生物相容性及骨引导活性，能促进骨缺损的早期修复，是一种颇有潜力的骨缺损修复材料。     

骨形成蛋白一多孔羟基磷灰石复合人工骨的实验研究

将牛骨形成蛋白(bBMP)与多孔羟基磷灰石(HA)结合制成 bBMP-HA 复合人工骨,植入狗股骨缺损区,植入后2,4,8,16,24周,采用组织学、扫描电子显微镜及~(43) Ca 同位素示踪综合考查其骨修复过程,并以单纯 HA 作为对照。结果发现,各实验程期 bBMP-HA 材料内新骨形成量均明显多于单纯 HA 对照组,bBMP-HA 植入后早期诱导骨形成较活跃,实验证明 bBMP-HA 复合人工骨具有良好的诱导成骨能力,并能促进骨缺损的修复。     

人工腭裂生物活性玻璃陶瓷植入的动物实验研究

对３０只小家犬手术造成人工腭裂，植入生物活性玻璃陶瓷（ＢＧＣ）与不植ＢＧＣ做对照，应用常规组织学方法、荧光显微镜及扫描电子显微镜技术对ＢＧＣ在腭部环境中以嵌贴方式植入后的愈合过程，组织相容性等进行研究。结果表明：ＢＧＣ在狗的腭部有良好的组织相容性，能引导新骨生成，对周围正常组织无不良干扰，是作为骨的替代材料整块植入修复腭裂全部骨缺损的较理想的材料。     

复合珊瑚骨修复颌骨实验性骨缺损

珊瑚人工骨具有良好的生物相容性，生物降解性和骨传导性；单纯植入机体后有定的体积丧失。本文将珊瑚骨和自体骨髓复合，修复兔下颌骨实验性骨缺损，术后不同时间行Ｘ线检查，＾９９ｍＴｃ－ＭＤＰ骨显像和组织学检查。     

含碳酸盐氟羟磷灰石—─兔骨内植入的动物试验观察

目的 :通过兔的骨内植入实验观察FCHA的成骨作用。方法 :用新西兰兔作为骨内植入模型 ,术后 2、4周处死。标本经病理切片、HE染色后 ,做组织学观察。结果 :FCHA、HA颗粒对骨的修复作用明显优于空白对照 ,FCHA、HA颗粒周围均有骨组织生成。FCHA周围新生骨的成骨量大于HA。结论 :FCHA、HA均有良好的生物相容性 ,且对骨缺损的修复有促进作用 ,前者的成骨作用明显优于后者。     

复合人工骨修复腭裂腭板缺损

目的 :在动物实验的基础上 ,实施以HA Bonecement复合人工骨作为植骨材料应用于临床腭板骨缺损的修复。方法 :就诊的有腭板骨缺损的先天性 Ⅱ°腭裂患者 4 0人 ,随机分为对照组和植骨组 ,植骨组腭板骨缺损利用HA Bonecement复合人工骨修复 ,术后 3个月复诊 ,植骨组患者行腭部CT扫描检查。结果 :腭部CT扫描显示植入人工骨愈合良好 ,植骨组语音改善明显优于对照组 (x2 =6 4 6,p <0 0 5 )。结论 :HA Bonecement复合人工骨可以作为临床腭板骨缺损修复材料 ;初步研究认为腭板骨缺损修复能改善腭咽闭合。     

高生物诱导活性复合人工骨整复骨缺损的实验研究

在建立家兔尺骨节段性骨缺损动物模型的基础上，首次采用羟基磷灰石－骨形成蛋白－胶原和羟基磷灰石－胶原两种不同复合人工骨材料分别整复骨缺损，经Ｘ线、大体、体视显微镜及组织学观察结果证实、ＨＡ－ｂＢＭＰ－Ｃ为复合人工骨骨有良好的组织相容性和显著的相诱导及骨引导作用在植入整复骨缺损区，其促使新骨形成的速度、数量和质量均显著优于ＨＡ－Ｃｏ复合人工骨，显示出良好的应用前景。     

致密多晶羟基磷灰石微粒人工骨-煅石膏复合材料的定量组织学研究

本研完将致密多晶羟基磷友石-煅石膏复合材料和单纯致密多晶羟基磷友石微粗人工骨分别植入10条狗下颌骨两侧骨缺损内．于不同时段获取标本，进行自身同期对照研完；通过计算机图像定量统计处理，分析二者新骨生长情况。结果表明．二者新骨生长量无明显差别，即煅石膏作为致密多晶羟基磷友石的粘结剂对后者的优良性能无不良影响。     

脱矿牙本质基质材料对大鼠颅骨缺损修复的研究

目的：探讨生物相容性较好的脱矿牙本质基质材料对大鼠颅骨缺损的修复效果。方法：①拔除健康大鼠上下前牙,脱矿,脱脂,粉碎后提取脱矿牙本质基质粉末,后与熟石膏以重量2：1比例混合。②大鼠头盖骨正中建立直径约8mm的圆形骨缺损,实验组：在缺损区植入脱矿牙本质基质粉末与熟石膏复合物,用生理盐水调和。对照组：无修复治疗。术后4周和8周分组处死取标本,进行大体标本, X线和组织学HE染色光镜下观察。结果：脱矿牙本质基质粉末与熟石膏具有良好的组织相容性和骨引导性,并与新骨紧密结合,熟石膏可以较好的抑制脱矿牙本质基质牙粉末颗粒离散。结论：脱矿牙本质基质粉末与熟石膏复合骨材料对大鼠颅骨缺损有良好的修复作用,可促进新骨形成,缩短骨缺损修复时间。     

致密多晶羟基磷灰石微粒人工骨-煅石膏复合材料的临床应用（31例报告）

在严格可靠的基础研究及动物实验前提下，将HA—PLP复合材料选择性地应用于临床，病倒包括牙槽嵴高度保持．颧部骨性塌陷畸形矫正，上、下领骨囊肿摘除骨腔填塞及鞍鼻成形等。所有病例无异常反应．伤口无HA微粒移漏，X-ray显示HA—PLP复合材料成形固位效果好。结果表明该复合材料具有良好的组织相容性，有良好的固位作用，对人体无毒、无副作用，是一种极有应用价值的骨缺损修复材料。此材料的应用还可降低医疗成本，具有较好的社会效益。     

Ridge augmentation using solid and porous hydroxylapatite particles with and without autogenous bone or plaster

Edentulous areas of dog jaws were augmented with solid or porous particles of hydroxylapatite (HA) alone, or combined with either finely crushed autogenous bone or plaster of paris. At the end of the experiment (24 weeks), the augmented ridges were firm and stable and covered with healthy mucosa. The ridges augmented with only porous particles of HA demonstrated a greater amount of bone ingrowth compared with the solid, dense particles. The new bone formation occurred in those parts of the implants adjacent to the underlying alveolar bone. The addition of autogenous bone to the HA particles did not enhance bony deposition, and none of the autogenous bone chips survived for 24 weeks. The amount of new bone in the ridges augmented with plaster of paris and HA was similar to the other groups, and the plaster did not interfere with healing. There was evidence of resorption of the underlying cortical bone in many of the specimens.     

煅石膏与骨形成蛋白载体系统的建立及其骨诱导活性研究

将牛骨形成蛋白(bBMP)与煅石膏(PLP)复合物及单纯PLP和单纯bBMp分别植入狗下颌的实验性骨缺损中，术后1，2，4和8用分别观察，发现PLP在植入后早期吸收，bBMP—PLP复合物有明显的骨诱导性．可防止BMP扩散及过快吸收，使其更持久地作用于周围的细胞。可在相对较短时间内．促进骨缺损的修复，研究表明．PIP是一种较适宜的BMP载体材料。     

Doxycycline-tricalcium phosphate composite graft facilitates osseous healing in advanced periodontal furcation defects

The purpose of this study was to compare the effectiveness of a tricalcium phosphate, plaster of paris, and doxycycline composite graft to surgical debridement alone in the treatment of Class II and Class III furcation defects. Fifteen patients with adult periodontitis and at least two mandibular molars with Class II or III furcation defects were selected. A total of 40 sites were treated: 26 were Class II defects and 14 were Class III. Following initial therapy one site was randomly selected to receive the composite graft while the remaining site served as the surgically debrided control. Osseous healing was evaluated by direct measurements from an acrylic stent at the time of graft surgery and at the 6-month reentry. Following surgery each patient was placed on doxycycline 100mg/day for 10 days. The absence of clinical inflammation and infection during the healing process provided additional substantiation of the biocompatibility of the grafting materials. Results after 6 months indicated that sites treated with the composite graft had improved defect fill, defect resolution, probing depths, and clinical attachment levels when compared to the surgically debrided controls. Defect fill was 3.7 times greater in grafted sites and these sites were 4.0 times more likely to have 50% or greater defect fill. The effect of grafting was more pronounced in Class III defects where horizontal defect fill and gain of clinical attachment was achieved only in grafted sites. The plaster of paris functioned well as a binder, preventing particle scatter and facilitating graft retention. Additionally the plaster served as a vehicle to carry and retain the doxycycline at the treated site. These short-term results point to the potential of a composite graft containing tricalcium phosphate, plaster of paris, and doxycycline in promoting healing of furcation lesions.     

载辛伐他汀纳米羟基磷灰石支架材料对兔下颌骨缺损修复的影响

目的:采用骨髓间充质干细胞(BMSCs)复合辛伐他汀支架材料修复兔下颌骨局部缺损,观察辛伐他汀对BMSCs复合支架材料修复兔骨缺损的影响。方法:24只新西兰大白兔随机分为2组,每组12只,制备下颌骨缺损模型。A组为实验组,在缺损区植入复合BMSCs的载辛伐他汀纳米羟基磷灰石支架材料;B组为对照组,植入BMSCs复合羟基磷灰石材料。分别于术后2、4、8、12周处死各组动物,行影像学分析、HE染色、扫描电镜观察等检查。结果:影像学检查及组织学染色结果显示,A组骨缺损处愈合程度、成骨速度及骨质量明显优于B组。扫描电镜显示,A组复合材料与组织相容性好,材料吸收优于B组。统计各组各期牙CT分析数据的骨密度值,结果表明A组的骨密度值明显高于B组(P<0.05)。结论:实验表明经BMSCs复合的辛伐他汀支架材料具有明显的促进成骨能力,可加速骨缺损的修复效果,提高修复质量。     

Grafting of large defects of the jaws with a particulate dentin-plaster of paris combination.

OBJECTIVE: The purpose of this report is to show the usefulness and discuss the effects of a particulate dentin and plaster of paris combination as a bone graft material in jaw defects. STUDY DESIGN: This was a retrospective evaluation of 10 patients with jaw defects who underwent grafting with a combination of particulate dentin and plaster of paris. The material was used when the defects were more than 20 mm in diameter. The ratio was 2:1 by weight. Patients were examined for any evidence of infection and recurrence during the follow-up periods. RESULTS: During a mean follow-up of 52.2 months (range, 50 to 57 months), patients had minor immediate postoperative complications. These complications were swelling and perforation; they were treated without problems through use of incision and drainage, antibiotic treatment, and buccal flap. CONCLUSIONS: On the basis of the results that we obtained radiographically and clinically, it may be concluded that the particulate dentin plaster mixture is a useful and readily available material for bone substitute.