Effect of Quyu Jiedu Granule (祛瘀解毒颗粒) on microenvironment of ova in patients with endometriosis

Objective:To observe the effect of Quyu Jiedu Granules(祛瘀解毒颗料,QJG) on the micro-environment of ova in patients with endometriosis(EM).Methods:Twenty EM patients who received in vitro fertilization and embryo transfer(IVF-ET) were randomized equally into a treated group and a control group. Further,20 patients who received IVF-ET due to oviduct factors were enrolled into a non-endometriosis group. The dosage of gonadotrophic hormone used,the number of ova attained,fertilization rate and clinical pregnancy...     

Study on clinical effect and mechanism of Jianpi Qingre Huayu Recipe (健脾清热化瘀方) in treating patients with gastric ulcer

Objective： To study the effects of Jianpi Qingre Huayu Recipe （健脾清热化瘀方, JQH） in curing gastric ulcer and to preliminarily probe into its pathogenic mechanism, Methods： Fifty patients with gastric ulcer of Pi （脾）-insufficiency and stasis-heat syndrome type were assigned to the treated group （30 patients） and the control group （20 patients）. They were treated respectively with JQH and Ranitidine. At the same time, another group consisting of 20 healthy persons was set up for normal control. The clinical effect on gastroscopic figure and traditional Chinese medicine （TCM） syndrome were observed. Changes of T-cell subsets and interleukin-8 （IL-8） in serum as well as IL-8 in mucosa around the gastric ulcer were determined before and after treatment by flow cytometry and ELISA. Results： Comparison of the total effective rate on gastroscopic figure in the treated group and the control group （86.7% vs 80.0%） showed insignificant difference, but the cure rate and markedly effective rate in the former （50.0% and 20.0%） was higher than that in the latter （40.0% and 15.0%） respectively. Comparison of the total effective rate on TCM syndrome in the treated group and in the control group （96.7% vs 70.0%） showed insignificant difference, but the cure rate and markedly effective rate in the former （63.3% and 23.3%） was higher than that in the latter （50.0% and 20.0%） respectively. Serum levels of CD3^＋, CD4^＋, CD8^＋ got restored to normal range in the treated group after treatment but it was not so in the control group. IL-8 level in gastric mucosa was improved in both groups but the improvement in the treated group was better. Cenclusien： JQH could effectively treat gastric ulcer and partly reduce its recurrence through improving patients＇ immune function.     

Effect of Nei-Yi Recipe (内异方) on plasma β-endorphin level during menstrual cycle in women with endometriosis

ＥｆｆｅｃｔｏｆＮｅｉ－ＹｉＲｅｃｉｐｅ（内异方）ｏｎＰｌａｓｍａβ－ＥｎｄｏｒｐｈｉｎｌｅｖｅｌｄｕｒｉｎｇＭｅｎｓｔｒｕａｌＣｙｃｌｅｉｎＷｏｍｅｎｗｉｔｈＥｎｄｏｍｅｔｒｉｏｓｉｓ　　ＥｆｆｅｃｔｏｆＮｅｉ－ＹｉＲｅｃｉｐｅ（内异方）ｏｎＰｌａ...     

Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia

Objective:To investigate the effects of Kangquan Recipe(康泉方,KQR)on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia(BPH)model in rats.Methods:Seventy-two SD rats were randomly divided into six groups:the normal group,the model group,the finasteride group,and the low-, middle-,and high-dose KQR groups,12 in each group.Except those in the normal group,the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively w...     

Effect of Combination of Bushen Jianpi Recipe (补肾健脾方) and Erythropoietin on Serum Tumor Necrosis Factor a in Patients with Anemia

Objective: To study the effect of treatment of renal anemia by combination of Bushen Jianpi Recipe (补贤健脾方, BJR, a Chinese experienced recipe for supplementing Shen and supporting Pi) and low dosage erythropoietin (EPO), and the influence of treatment on change of serum tumor necrosis factor α (TNF-α) so as to explore the possible mechanism of integrative Chinese and Western medicine (ICWM) in treating renal anemia. Methods: Patients with renal anemia were randomly divided into two groups, the ICWM group and the control group, supporting treatment such as dialysis, supplementing of ferrous, foliac acid and vitamin B12, was given to both groups. Additionally, to the ICWM group, 50 IU/kg of EPO subcutaneous injection for twice every week, and oral intake of BJR, one dose per day taken in two parts, were given, and to the control group, EPO alone, 50IU/kg by subcutaneous injecting, 3 times perweek, was given. The therapeutic course for two groups was 3 months. Blood levels of hemoglobin (Hb), hematoc     

Clinical Effect of Fuzheng Jiedu Granule (扶正解毒颗粒) on Serum NO,NOS and Microelements in Patients with Coronary Heart Disease in Long-term Contact with Nickel

Objective: To study the clinical effect of Fuzheng Jiedu granule (扶正解毒颗粒, FJG) and its influence on serum nitric oxide (NO), nitric oxide synthase (NOS) and rnicroelements in patients with coronary heart disease who are in long-term contact with nickel. Methods: Sixty patients were randomly divided into two groups, the treated group and the control group, 30 in each group. Conventional Western medicinal treatment was applied to both groups, but to the treated group, FJG was given additionally by oral intake, and the therapeutic course for both groups was 4 weeks. Besides, a health control group was set up with 15 healthy subjects selected from the same area, who had no history of contact with nickel. Clinical symptoms of the patients and changes in their electrocardiogram (ECG) and serum levels of NO, NOS and microelements were observed before and after treatment. Results: The total effective rate on clinical symptoms in the treated group was 93.3% and that in the control group 63.3%, comparison between the two showing significant difference (P＜0. 05); the total effective rate on ECG in the two groups was 90.0% and 56.7% respectively, which also showed significant difference ( P＜0. 05). After treatment, levels of serum NO, NOS, Mg, Zn and Ca increased and those of Ni, Mn and Fe decreased significantly ( P＜0. 05 or P＜0.01) in the treated group, while in the control group, changes in these laboratory indexes were insignificant. Conclusion: FJG could lower the blood nickel level and regulate the unbalance of microelements, help restore serum NO and NOS in persons exposed to nickel and improve the clinical symptoms of patients with coronary heart disease and in long-term contact with nickel.     

Effect of Tiangui Recipe (天癸方) on Serum Leptin and Pituitary Gonadotro pin in Androgen-Sterilized Rats

Ｓｕｆｆｉｃｉｅｎｔｎｕｔｒｉｔｉｏｎ，ｃｅｒｔａｉｎｅｎｅｒｇｙｒｅｓｅｒｖｏｉｒａｎｄｌｉｐｉｄｓａｒｅｎｅｃｅｓｓａｒｙ（1）ｆｏｒｉｎｉｔｉａｔｉｎｇａｎｄｍａｉｎｔａｉｎｉｎｇｔｈｅｒｅｐｒｏｄｕｃｔｉｖｅｆｕｎｃｔｉｏｎｏｆａｎｉｍａｌｓａｎｄ     

Effects of Xuesetong Soft Capsules(血塞通软胶囊) on angiogenesis and VEGF mRNA expression in ischemic myocardium in rats with myocardial infarction

OBJECTIVE:To observe the effects of Xuesetong Soft Capsules(血塞通软胶囊,Notoginseng total saponin) on angiogenesis and vascular endothelial growth factor(VEGF) mRNA expression in ischemic myocardium of rats with myocardial infarction.METHODS:The left coronary artery of rats was ligated to establish the animal model of acute myocardial infarction.Rats were randomly divided into Xuesetong Soft Capsule,Shexiangbaoxin Pill(positive control),model(negative control) and sham operation groups.After 6 weeks,microvessel count(MVC),microvessel density(MVD) and VEGF mRNA expressioninischemicmyoc ardium were evaluated.RESULTS:MVC and MVD in the myocardial infarct border area in model,Shexiangbaoxin Pill and Xuesetong Soft Capsule groups significantly increased compared with those of the sham operation group(P<0.05).MVC and MVD in the myocardial infarct border area in Xuesetong Soft Capsule and Shexiangbaoxin Pill groups significantly increased compared with those of the model group(P<0.05).No significant differences between Xuesetong Soft Capsule and Shexiangbaoxin Pill groups were observed(P>0.05).The model group showed signifi-cantly higher VEGF mRNA expression than that in the sham operation group(P<0.05).Xuesetong Soft Capsule and Shexiangbaoxin Pill groups showed significantly higher VEGF mRNA expression than that of the model group(P<0.05).No significant difference between Xuesetong Soft Capsule and the Shexiangbaoxin Pill groups was observed(P>0.05).CONCLUSION:Xuesetong Soft Capsules promote angiogenesis in ischemic myocardium after myocardial infarction and the mechanism may be associated withVEGF mRNA expression.     

Effect of Yiqi Huoxue Recipe (益气活血方) on cardiac function and ultrastructure in regression of pressure overload-induced myocardial hypertrophy in rats

Objective:To investigate the effect of Yiqi Huoxue Recipe (YHR,益气活血方)on the cardiac function and ultrastructure during the regression of myocardial hypertrophy induced by pressure overload in rats.Methods:The model of myocardial hypertrophy was established by abdominal aortic banding.Eighty male Wistar rats were divided into six groups,the normal control groupⅠ(n=20),the normal control groupⅡ(n=12),the hypertension model groupⅠ(n=12),the hypertension model groupⅡ(n=12),the YHR group(n=12)and the Captopril group(n=12).The observation was carried out in the normal control groupⅠand the hypertension model groupⅠafter 4 weeks of modeling,and the other four groups were observed after 16 weeks of modeling(12 weeks of administration).The cardiac function was measured with a multichannel biological signal analysis system,and the myocardium ultrastructure was observed by a transmission electron microscope. Results:(1)Compared with the normal control groupⅠ,the systolic blood pressure and cardiac coefficient(left ventricular weight/body weight)in the modelⅠgroup was higher(P<0.05,P<0.01). (2)In the YHR group,cardiac coefficient and-dp/dt_(max)were lower,left ventricular systolic pressure and dp/dt_(min)were higher when compared with the model groupⅡand the Captopril group(P<0.05 or P<0.01).In the Captopril group,only cardiac coefficient was lower when compared with the mode groupⅡ(P<0.05).(3)Compared with the normal control groupⅡ, dp/dt_(max)was higher(P<0.01), -dp/dt_(max)and isovolumetric contraction time(ICT)was lower(P<0.05,P<0.01)in both the YHR group and the Captopril group.(4)Results of the myocardium ultrastructure showed edema under myocardium plasmalemma,enlarged sarcoplasmic reticulum and T tube,and significantly enlarged intercalated disc of the cardiac muscle in the model groups.In the Captopril group,the extension of sarcoplasmic reticulum and T tube as well as the pathological changes of intercalated disc were lighter,with slight edema under the myocardium plasmalemma.In the YHR group,the expansion of the sarcoplasmic reticulum was less than in the Captopril group,part of the pathological changes of intercalated discs was slightly more severe than that in the Captopril group,the dissolution of nuclear chromatin was not found,which was similar to that of the Captopril group,and no injury of the nucleus was found,either.Conclusion:YHR could reverse myocardial hypertrophy in rats with abdominal aortic banding and improve the systolic and diastolic function of the left ventricle.The ultrastructure of the myocardium such as arcoplasmic reticulum,intercalated disc,and cell nucleus in abdominal aortic banding rats could be partly reversed by the recipe.     

子宫内膜异位症患者异位子宫内膜VEGF表达的研究

目的 研究子宫内膜异位症（EM）患者异位子宫内膜VEGF的表达及其在EM发病机制中的作用。方法 应用 ELISA法检测有或无EM的妇女腹腔液中的血管内皮生长因子（VEGF）水平、采用免疫组织化学S-P法分别测定VEGF及其受体KDR在EM患者异位与在位内膜及对照组子宫内膜组织中的表达,并以抗第八因子相关抗原（F8-RA）抗体标记间质微血管并进行微血管计数（MVD）。结果 EM患者腹腔液中VEGF水平明显高于对照组;VEGF与其受体在内膜腺上皮细胞及部分血管内皮细胞上存在的共同表达。结论 VEGF参与了EM的发病机制,自分泌或旁分泌是VEGF促进血管生成的重要途径。     

uPA、Ki-67和VEGF在宫颈癌组织中的表达及相关性研究

目的 探讨尿激酶型纤溶酶原激活物(uPA)、增殖细胞核抗原(Ki-67)和血管内皮生长因子(VEGF)在宫颈浸润癌组织中的表达及与临床病理参数的关系.方法 采用免疫组织化学SP法,分别检测uPA、Ki-67及VEGF蛋白在94例宫颈浸润癌(ICC),83例宫颈上皮内瘤样病变(CIN)和23例正常宫颈上皮(NCE)中的表达,并分析其与宫颈癌临床病理学参数间的关系及三者间的相关性.结果 免疫组化方法显示:从NCE到CIN再到ICC,uPA蛋白阳性表达率分别为8.7％、44.6％和71.3％;VEGF蛋白阳性表达率分别为17.4％、43.4％和76.6％;Ki-67蛋白阳性表达率分别为13.0％、34.9％和67.0％;差异均有统计学意义(P＜0.01).uPA和VEGF在ICC中的表达与盆腔淋巴结转移和临床分期有关(P＜0.01);而与年龄和病理学类型、组织学分级无关(P＞0.05);Ki-67在ICC中的阳性表达与临床分期有关(P＜0.01),而与年龄、盆腔淋巴结转移、病理学类型及组织学分级无关(P＞0.05).在宫颈癌组织中,uPA、Ki-67及VEGF蛋白表达两两间均呈显著正相关(r=0.705,P＜0.01;r=0.871,P＜0.01;r=0.628,P＜0.01).结论 uPA、Ki-67及VEGF与宫颈癌的发生、发展密切相关,联合检测这些指标有助于判断宫颈癌的预后及干预治疗.     

VEGF、MMP-7在子宫内膜异位症中的表达及意义

目的探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）、基质金属蛋白酶-7（mauixmet-alloproteinases-7,MMP-7）在在正常人及子宫内膜异位症（endometriosis,EMT）患者内膜组织中的表达,探讨VEGF、MMP-7在EMT发生发展中的作用。方法通过免疫组化S-P法分别检测MMP-7及VEGF在EMT患者在位子宫内膜及异位内膜和正常子宫内膜中的表达。结果异位内膜MMP-7表达与在位内膜无差异,但高于正常内膜（P〈0.05）,在位内膜与正常子宫内膜相比,差异有显著性（P〈0.05）;卵巢异位内膜VEGF表达与同期在位内膜相比,表达增高,差异有显著性（P〈0.05）,且明显高于正常内膜（P〈0.005）;在位内膜与正常子宫内膜相比,VEGF表达差异无显著性（P〉0.05）。结论 EMT在位内膜、异位内膜中VEGF及MMP-7与异位内膜的高侵袭性和高血管新生能力有关,在EMs的发生发展中起重要作用。     

子宫内膜异位症患者腹腔液和在位子宫内膜血管内皮生长因子表达的研究

目的 :探讨血管内皮生长因子 (VEGF)在子宫内膜异位症 (EMT)发病中的意义。方法 :用酶联免疫吸附法测定 1 9例EMT患者和 2 1例正常妇女 (对照组 )腹腔液中VEGF水平。用相对定量RT PCR测定 1 5例EMT患者和 1 2例正常对照妇女在位子宫内膜VEGF的表达水平。结果 :EMT组腹腔液VEGF水平较正常对照组显著升高(P <0 .0 5 ) ,EMT组VEGF增殖期较分泌期升高 (P <0 .0 5 ) ,对照组无周期性变化 (P >0 .0 5 ) ;EMT组的在位子宫内膜VEGF的表达明显高于对照组 (P <0 .0 0 1 ) ,增殖期和分泌期两者差异无显著性 (P >0 .0 5 )。结论 :EMT患者腹腔液和在位子宫内膜VEGF升高 ,可能对EMT的发生发展具有重要意义     

米非司酮治疗后VEGF-165在异位和在位子宫内膜中的表达

目的探讨米非司酮对VEGF-165在子宫腺肌症中表达的影响。方法43例子宫腺肌症患者分为对照组(n=21)和米非司酮治疗组(n=22),采用放射免疫法测定对照组(月经第3天)和米非司酮组(术前)血清中FSH、LH、E2、PRL、P及T的水平。采用免疫组化法测定两组患者在在位和异位子宫内膜中的VEGF-165蛋白水平。结果米非司酮组较对照组血清FSH、LH、E2、P明显降低(P<0.05),而血清PRL和T的水平两组间无统计学差异(P>0.05)。VEGF-165在子宫内膜腺上皮细胞内的表达,异位内膜均明显高于在位内膜(P<0.05);而其在间质细胞内的表达,异位内膜与在位内膜无统计学差异(P>0.05)。米非司酮组异位和在位内膜腺上皮细胞、间质细胞中VEGF-165的表达水平均较对照组明显降低(P<0.05)。VEGF-165在对照组异位内膜腺上皮细胞中的表达,增殖期高于分泌期(P<0.05)。结论米非司酮治疗后,VEGF-165在异位和在位内膜中的表达明显下降,可能是通过抑制VEGF蛋白合成,降低血管通透性,抑制新生血管生成,从而有效地控制子宫内膜异位症的发生发展。     

血管内皮生长因子在异位和在位子宫内膜中的表达

目的研究血管内皮生长因子（vascular endothelial growth factor,VEGF）在子宫内膜异位症（endometrio-sis,EM）患者的异位和在位内膜中的表达及其在EM发病机制中的作用。方法应用ELISA法检测有或无EM的妇女的血管内皮生长因子（VEGF）水平、采用免疫组织化学S-P法测定VEGF在EM患者异位与在位内膜及对照组增殖期和分泌期子宫内膜组织中的表达,并以抗第八因子相关抗原（F8-RA）抗体标记间质微血管并进行微血管计数（MVD）。结果在整个月经周期中,研究组异位和在位内膜中VEGF表达均持续高于对照组（P〈0.01）。EMs患者异位内膜和在位内膜的微血管密度（MVD）明显高于对照组内膜（P〈0.05）。结论VEGF在EMs患者的高表达,说明其通过促血管生成在该病发生发展中起着重要作用。     

Ki-67和VEGF在肾细胞癌中的表达及意义

目的探讨肿瘤细胞核增殖抗原（Ki-67）和血管内皮生长因子（VEGF）在肾细胞癌（RCC）中的表达及在肾癌发生、发展过程中的作用。方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。结果Ki-67mRNA和VEGFmRNA在肾癌组织中表达显著高于正常肾组织（χ^2=28.010,P〈0.05和χ^2=15.539,P〈0.05）,且两者都与肾癌临床病理参数存在相关性。结论Ki-67与VEGF在肾癌组织中高表达,且呈明显的正相关性,两者在肾癌细胞增殖及转移中起了重要作用。     

川芎嗪注射液对子宫内膜异位症术后复发者治疗前、后血中VEGF的影响

目的:探讨川芎嗪注射液对子宫内膜异位症术后复发者治疗前、后血中血管内皮生长因子(VEGF)的影响。方法:子宫内膜异位症术后复发患者60例,随机分为观察组和对照组,每组30例,正常体检妇女15例作为正常对照组。两组均给予米非司酮治疗,观察组加用川芎嗪注射液,连续用药12周,观察不良反应及血清E2水平。采用正常对照组体检时、两组患者均于治疗前及治疗后空腹抽血5mL,采用ELISA法定量检测VEGF。结果:观察组治疗子宫内膜异位症术后复发患者痛经、闭经改善优于对照组(P<0.01),使血清E2保持在卵泡期水平,副作用少。治疗后两组VEGF水平均下降,与治疗前有统计学差异(P<0.01),两组治疗后亦有显著性差异(P<0.01)。结论:川芎嗪注射液治疗子宫内膜异位症术后复发患者比单用米非司酮副作用少、安全性高,患者易于接受,且不出现雌激素缺乏症状,停药后排卵功能恢复快,月经复潮时间短,有利于早日受孕,VEGF水平可作为子宫内膜异位症术后复发的监测指标。     

Ki-67蛋白和VEGF及bFGF在恶性淋巴瘤中的表达及其意义

目的 探讨恶性淋巴瘤(ML)患者组织中Ki-67蛋白、血管内皮生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)的表达,分析其在疾病中的意义. 方法 采用免疫组化SP法测定42例ML患者、50例健康者(正常对照组)和16例淋巴结反应性增生者的Ki-67蛋白、VEGF及bFGF的表达. 结果 淋巴瘤患者Ki-67蛋白、 VEGF、bFGF的表达率分别为52.38%、42.85%、45.24%,显著高于淋巴结反应性增生组及正常对照组(P＜0.05);不同性别、细胞类型及恶性程度患者Ki-67蛋白、VEGF及bFGF的阳性表达率间差异均无统计学意蚁(P＞0.05),而在低年龄组及临床分期Ⅲ+Ⅳ期组Ki-67蛋白、VEGF及bFGF的阳性表达率高于高年龄组(bFGF除外)及临床分期Ⅰ+Ⅱ期组(P＜0.05).结论 Ki-67蛋白、VEGF及bFGF的异常表达可能在疾病的发病中起一定的作用,三者联合检测有助于判断疾病分期及预后.     

VEGF在子宫内膜异位症中的表达

目的 通过检测子宫内膜异位症（EM）患者体内血管内皮生长因子（VEGF）的表达，探讨它在子宫内膜异位症发生发展中的作用。方法 应用ELISA测定血清和腹腔液中VEGF的含量；免疫组织化学测定正常子宫内膜与患者在位、异位子宫内膜组织中VEGF的表达。结果 ①血清、腹腔液VEGF的水平，EM各组高于正常对照组（P〈0．01）；EM早期组高于晚期组（P〈0．05）；各组腹腔液VEGF水平均高于血清水平（P〈0．05）。异位内膜组、在位内膜组VEGF的表达分别与对照组比较显著增高（P〈0．05），在异位内膜组中Ⅲ、Ⅳ期组与Ⅰ、Ⅱ期组相比明显降低（P〈0．01）。②患者的异位内膜组VEGF的表达和腹腔液组VEGF的含量呈正相关（P〈0．05；rs=0．404）。结论 患者体内VEGF的异常表达在子宫内膜异位症的发生发展中起重要的作用。