p53、EGFR和VEGF在脑胶质瘤中的表达及相关性研究

目的 :探讨抑癌基因蛋白 (p5 3)、表皮生长因子受体 (EGFR)和血管内皮生长因子 (VEGF)的表达、相互关系及其意义。方法 :采用免疫组织化学 (S P)法 ,检测 15 0例脑胶质瘤标本中 p5 3、EGFR和VEGF的表达。 结果 :p5 3、EGFR和VEGF在胶质瘤中的阳性表达率 5 4 .0 %、5 2 .0 %和 4 4 .7% ,并随胶质瘤病理分级的增加而增高 ,差异有显著性 (P <0 .0 5 )。p5 3和EGFR、EGFR和VEGF表达间均存在着密切关系 (P <0 .0 0 5 )。结论 :p5 3、EGFR和VEGF高表达与胶质瘤的恶性进展有密切关系 ;且 p5 3与EGFR、EGFR与VEGF协同在胶质瘤发生、发展过程中起重要作用。     

神经胶质瘤中SPARC、P53和VEGF的表达及临床意义

目的：通过检测富含半胱氨酸的酸性分泌蛋白( SPARC)、P53和血管内皮细胞生长因子( VEGF)在神经胶质瘤中的表达,探讨三者之间的相关性及其临床意义。方法依据神经胶质瘤的分级选取神经胶质瘤患者90例和瘤旁脑组织30例,采用免疫组织化学方法检测SPARC、P53和VEGF在神经胶质瘤不同病理分级中的表达情况,进行临床病理相关性分析。结果①在胶质瘤中 VEGF、P53的阳性表达率分别是75.56%和68.89%,均显著高于瘤旁脑组织中的阳性表达率(20.00%和0),而SPARC的阳性表达率在胶质瘤低于瘤旁组织(24.44% vs 80.00%,P<0.01)。②SPARC的阳性表达率随着胶质瘤恶性程度的增高而降低(P<0.05);且阳性和阴性患者生存率分析差异无统计学意义。③VEGF的阳性表达率随着胶质瘤恶性程度的增高而增高(P<0.01),其阳性和阴性患者生存率差异有统计学意义。④P53的阳性表达率与肿瘤病理分级中差异无统计学意义;其阳性和阴性患者生存率差异有统计学意义。⑤在神经胶质瘤组织中,P53和VEGF的表达呈正相关,SPARC与 P53、VEGF 的表达均呈负相关。结论①SPARC 在神经胶质瘤中的阳性表达率显著低于瘤旁脑组织。②SPARC表达下调和P53、VEGF表达上调与神经胶质瘤的发生、发展及预后有关。     

神经胶质瘤中SPARC、P53和VEGF的表达及临床意义

目的 通过检测富含半胱氨酸的酸性分泌蛋白(SPARC)、P53和血管内皮细胞生长因子(VEGF)在神经胶质瘤中的表达,探讨三者之间的相关性及其临床意义. 方法 依据神经胶质瘤的分级选取神经胶质瘤患者90例和瘤旁脑组织30例,采用免疫组织化学方法检测SPARC、P53和VEGF在神经胶质瘤不同病理分级中的表达情况,进行临床病理相关性分析.结果 ①在胶质瘤中VEGF、P53的阳性表达率分别是75.56％和68.89％,均显著高于瘤旁脑组织中的阳性表达率(20.00％和0),而SPARC的阳性表达率在胶质瘤低于瘤旁组织(24.44％ vs 80.00％,P＜0.01).②SPARC的阳性表达率随着胶质瘤恶性程度的增高而降低(P＜0.05);且阳性和阴性患者生存率分析差异无统计学意义.③VEGF的阳性表达率随着胶质瘤恶性程度的增高而增高(P＜0.01),其阳性和阴性患者生存率差异有统计学意义.④P53的阳性表达率与肿瘤病理分级中差异无统计学意义;其阳性和阴性患者生存率差异有统计学意义.⑤在神经胶质瘤组织中,P53和VEGF的表达呈正相关,SPARC与P53、VEGF的表达均呈负相关. 结论 ①SPARC在神经胶质瘤中的阳性表达率显著低于瘤旁脑组织.②SPARC表达下调和P53、VEGF表达上调与神经胶质瘤的发生、发展及预后有关.     

VEGF和MMP-7在不同病理级别胶质瘤中的表达及临床意义

目的探讨不同病理级别胶质瘤组织中VEGF、MMP-7的表达及其临床意义。方法采用二步法免疫组化检测10例正常脑组织和78例胶质瘤组织中VEGF,MMP-7的表达,并与胶质瘤的恶性程度进行相关性分析。结果VEGF、MMP-7在正常脑组织中表达均为阴性,在不同病理级别胶质瘤中均有表达,并随肿瘤病理级别增高而增高,分别与正常脑组织比较差异均有统计学意义（P〈0．01）;相关分析发现VEGF表达与MMP-7的表达呈显著正相关（P〈0．01）。结论提示VEGF在细胞质内的集聚可促进MMP-7的表达,二者与胶质瘤的发生、发展、侵袭性有关,可反映胶质瘤的恶性程度和病理分级及预后。     

儿童脑胶质瘤组织中P27表达与病理分级、患儿预后的相关性研究

目的　探讨P2 7在儿童脑胶质瘤中的表达及与其发生、发展和患儿预后的关系。方法　应用免疫组织化学S -P法 ,检测 48例儿童脑胶质瘤和 9例正常脑组织中P2 7蛋白的表达 ,并结合长期随访资料进行分析。结果　P2 7在儿童脑胶质瘤阳性表达 (58.3 % )明显低于正常组 (1 0 0 % ) (P <0 .0 5) ;P2 7表达与儿童脑胶质瘤病理分级呈负相关 (r=0 .51 5 ,P <0 .0 1 ) ,随着肿瘤恶性程度的增高 ,P2 7表达呈降低态势 ;P2 7在低度恶性组与高度恶性组表达间有显著差异 (P <0 .0 5) ;P2 7的表达与脑胶质瘤的预后呈正相关 ,P2 7高表达组 ,患儿生存率高 ,反之则下降。结论　P2 7表达水平的降低在儿童脑胶质瘤发生、发展起重要作用 ,可作为判断儿童脑胶质瘤恶性程度及预测预后的参考指标。     

ICAM-1及VEGF在脑胶质瘤中的表达及其与临床预后的关系

目的 探讨细胞间黏附分子-1(ICAM-1)及血管内皮生长因子（VEGF）在脑胶质瘤中的表达及其与临床预后的关系。方法 收集2015年6月至2018年1月广西壮族自治区南溪山医院手术切除的85例脑胶质瘤组织为观察组,25例颅脑外伤患者的正常脑组织为对照组。对比2组标本中ICAM-1及VEGF表达差异,分析肿瘤组织中ICAM-1及VEGF表达与各病理参数的相关性,绘制Keplan-Meier生存曲线分析ICAM-1及VEGF表达对生存预后的影响。结果 观察组ICAM-1及VEGF高表达率较对照组显著升高（P<0.05）。病理分级Ⅲ～Ⅳ级、低分化患者ICAM-1及VEGF高表达率明显高于Ⅰ～Ⅱ分级和高、中分化患者（P<0.05）。病理分级Ⅲ～Ⅳ级、低组织分化、ICAM-1及VEGF高表达是影响脑胶质瘤患者预后状况的独立危险因素（P<0.05）。ICAM-1高表达组、VEGF高表达组患者平均生存时间较低表达组明显缩短（P<0.05）。结论 ICAM-1、VEGF在脑胶质瘤中呈高表达状态,其表达量与脑胶质瘤生物学特征及患者预后密切相关。     

VEGF和NGF在人脑胶质瘤的表达变化

目的 研究血管内皮细胞生长因子(VEGF)和神经生长因子(NGF)在胶质瘤内的表达,探讨它们与胶质瘤病理分级的关系及其在胶质瘤发生过程中的作用.方法 通过免疫组织化学SP法和免疫印迹技术检测5例正常脑组织和20例胶质瘤中VEGF和NGF的表达.结果 VEGF和NGF在胶质瘤及正常脑组织内均有表达.VEGF主要分布于胶质瘤细胞的细胞浆,而NGF在胶质瘤细胞胞核和胞浆均有表达.免疫印迹结果显示:VEGF和NGF在胶质瘤中的蛋白水平均高于正常脑组织,并且随着胶质瘤病理级别(Ⅰ～Ⅳ)的增高蛋白表达逐渐增加,差异均有统计学意义(P<0.05).结论 VEGF和NGF在胶质瘤内的表达均高于正常脑组织, 且随着病理级别的增高而增加,提示VEGF和NGF在胶质瘤的发生过程中可能起重要的作用,其水平高低与胶质瘤的恶性程度有关.     

脑胶质瘤中血小板衍生生长因子和血管内皮生长因子表达的协同效应

目的：研究血小板衍生生长因子（PDGF）和血管内皮生长因子（VEGF）在脑胶质瘤中的表达及相关性,探讨其在人脑胶质瘤发生、发展和侵袭中的意义。方法：采用免疫组织化学S-P法,检测脑胶质瘤60例、正常脑组织10例中PDGF和VEGF的表达。结果：（1）PDGF在胶质瘤60例中表达30例（50．0％1,不同级别胶质瘤间的表达有差异,其表达与胶质瘤病理分级呈正相关（r=0．5807,P〈O．01）;（2）VEGF在胶质瘤中表达34例（56．7％）,不同级别胶质瘤表达与胶质瘤病理分级呈正相关（r=O．5373,P〈0．01）;（3）PDGF与VEGF在脑胶质瘤中的表达呈正相关fr=0．7715,P〈0．01）。结论：PDGF、VEGF的表达水平随着胶质瘤级别的增加而升高,与胶质瘤的病理学分级呈正相关,与胶质瘤进展密切相关,是恶性程度增高标志,两者在胶质瘤发生、发展中起协同作用。     

Smo、Gli2及FoxM1对神经胶质瘤诊断和预后的评估价值

目的探讨Smo、胶质瘤相关癌基因同源蛋白2(Gli2)及叉头框转录因子M1(FoxM1)对神经胶质瘤诊断和预后的评估价值。 方法取80例神经胶质瘤标本为神经胶质瘤组,60例正常脑组织标本为正常组。免疫组化法检测两组Smo、Gli2、FoxM1表达水平,分析其与神经胶质瘤患者临床病理特征的关系;Kaplan-Meier生存曲线分析不同Smo、Gli2、FoxM1表达水平神经胶质瘤患者预后的差异,分析影响神经胶质瘤预后的相关因素。 结果神经胶质瘤组织Smo、Gli2、FoxM1高表达率均高于正常脑组织,且随神经胶质瘤患者组织学分级升高而表达率增高(P＜0.05)。不同组织学分级、不同Smo、Gli2、FoxM1表达水平的神经胶质瘤患者5年生存率存在显著差异(P＜0.05)。组织学Ⅲ~Ⅳ级、Smo高表达、Gli2高表达、FoxM1高表达是神经胶质瘤预后的危险因素(P＜0.05)。 结论Smo、Gli2、FoxM1高表达是神经胶质瘤预后的危险因素,三者有望作为神经胶质瘤诊断和预后的判断指标。     

HIF-1α、VEGF表达与脑胶质瘤预后的相关性

目的　探讨缺氧诱导因子 - 1α(HIF - 1α)、血管内皮生长因子 (VEGF)表达对人脑胶质瘤预后的影响。方法　采用免疫组织化学方法检测 6 3例脑胶质瘤HIF - 1α、VEGF的表达状况 ,并将结果与临床预后随访结果进行综合分析。结果　HIF - 1α总阳性表达率为 71.4 % ,其中强表达 16例 (2 5 .4 % ) ,中度表达 14例 (2 2 .2 % ) ,弱表达 15例 (2 5 .4 % ) ,不表达 18例 (2 8.6 % ) ;与正常对照组比较有显著性差异 (P <0 .0 1)。HIF - 1α表达水平与胶质瘤恶性程度相关 (r =0 .4 6 7,P <0 .0 1)。HIF - 1α主要表达于肿瘤边缘或与正常组织交界处的细胞及坏死的肿瘤组织中。不同组织学分级的脑胶质瘤标本肿瘤细胞质均有VEGF表达 ,胶质瘤Ⅰ级、Ⅱ级、Ⅲ～Ⅳ级VEGF阳性细胞数分别为 175± 6 6、35 2± 10 0、6 6 2± 12 3(个 ) ,3组比较有显著性差异 (P <0 .0 1)。VEGF与HIF - 1α表达水平有关 (r=0 .817,P <0 .0 1)。患者生存时间与HIF - 1α呈负相关 (r =- 0 .82 4 ,P <0 .0 1)。结论　VEGF与HIF - 1α表达有一定的相关性 ,HIF - 1α与脑胶质瘤新生血管生成有关。HIF - 1α、VEGF联合检测可更准确判断患者的预后。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.