清肺口服液对呼吸道合胞病毒增殖影响的研究

目的:观察清肺口服液含药血清对呼吸道合胞病毒(Respiratory Syncytial Virus RSV)A、B亚型增殖的影响.方法:采用组织培养病毒滴度测定方法观察呼吸道合胞病毒A、B亚型滴度的变化.结果:清肺口服液含药血清不能影响呼吸道合胞病毒A、B亚型滴度.结论:清肺口服液含药血清不能直接影响呼吸道合胞病毒增殖.     

重组人干扰素α-2b体外抗呼吸道合胞病毒作用的实验研究

目的观察重组人干扰素α-2b在体外的抗呼吸道合胞病毒作用。方法观察不同浓度重组人干扰素α-2b抑制呼吸道合胞病毒对Hela细胞产生细胞病变（CPE）的作用,并与利巴韦林比较。结果实验表明重组人干扰素α-2b抗呼吸道合胞病毒的最低有效浓度为25IU／ml。结论重组人干扰素α-2b具有明显的抗呼吸道合胞病毒作用。     

呼吸道合胞病毒对人支气管上皮细胞通透性的影响

目的 探讨呼吸道合胞病毒对支气管上皮细胞通透性的影响.方法 通过ELISA、荧光定量PCR检测呼吸道合胞病毒感染人支气管上皮细胞后对其血管内皮生长因子(VEGF)表达的影响,同时,通过检测细胞的跨膜电阻了解呼吸道合胞病毒对人支气管上皮细胞通透性的影响.结果 呼吸道合胞病毒增加了人支气管上皮细胞VEGF的表达,增加了支气管上皮细胞的通透性,VEGF的单克隆抗体可以明显阻断这一效应.结论 呼吸道合胞病毒通过上调VEGF的表达增加对支气管上皮细胞的通透性.     

小儿呼吸道感染性疾病三种病原的检测与分析

目的 探讨海口地区呼吸道感染疾病中肺炎支原体、腺病毒、呼吸道合胞病毒发病率的情况。方法 用固相酶联免疫吸附(ELISA)方法,捡测血清x2的特异性IgM抗体。结果 上呼吸道感染中肺炎支原体发病率占24.14%,下呼吸道感染中肺炎支原体发病率占31.9%,上呼吸道感染中腺病毒感染率占24.14%,下呼吸道感染中腺病毒感染率占18.89%,上呼吸道感染中呼吸道合胞病毒发病率占15.17%,下呼吸道感染中呼吸道合胞病毒发病率占17.24%。结论 肺炎支原体、腺病毒、呼吸道合胞病毒是小儿呼吸道感染的主要病原。     

2009~2010年广州地区儿童呼吸道合胞病毒感染分析

目的 应用实时荧光PCR方法检测呼吸道合胞病毒,分析2009~2010年广州地区儿童感染呼吸道合胞病毒的状况。方法 以呼吸道合胞病毒的N基因序列为参考,设计特异性引物和TaqMan荧光探针,建立实时荧光PCR方法,进行灵敏度和特异性检测。采用实时荧光PCR方法,对2009~2010年两年本院门诊或住院部急性呼吸道感染患儿的21458份咽拭子样本进行了检测。结果 （1）实时荧光PCR方法具有较好的特异性和灵敏度,与其他常见呼吸道病毒无交叉反应。（2）2009年的10477例临床标本中实时荧光PCR方法检测全年的呼吸道合胞病毒阳性率为7.25％,流行高峰在3月份,感染率为20.04%;2010年检测的10981例临床标本中,呼吸道合胞病毒阳性率为9.73％,有两个流行高峰,一个在2月,感染率为29.56％,另一个高峰在12月份,感染率达到22.20％。结论 本研究建立的实时荧光PCR方法具有简便快捷、特异、敏感的特点,适用于临床呼吸道合胞病毒的检测。2009~2010年呼吸道合胞病毒依然是广州地区儿童急性呼吸道感染的主要病原。     

利巴韦林联合清肺祛瘀汤治疗小儿呼吸道合胞病毒性肺炎

目的比较清肺祛瘀汤与利巴韦林对呼吸道合胞病毒性肺炎患儿治疗效果的差异,观察联合应用两者对小儿呼吸道合胞病毒性肺炎患者的疗效。方法选择2008年5月～2009年5月在我院治疗的小儿呼吸道合胞病毒肺炎107例,随机分为联合应用组与清肺祛瘀汤组、利巴韦林组,比较三组疗效。结果清肺祛瘀汤组发热患儿体温恢复正常时间及积分改善均比利巴韦林组效果好,但利巴韦林组的呼吸道合胞病毒转阴时间优于清肺祛瘀汤治疗组。联合应用组疗效优于清肺祛瘀汤组和利巴韦林组。结论利巴韦林与清肺祛瘀汤治疗小儿呼吸道合胞病毒性肺炎都有一定积极作用,但单独使用的效果不及联合应用,因此联合应用两者治疗小儿呼吸道合胞病毒性肺炎是一种行之有效的安全方法 。     

碱性磷酸酯抗碱性磷酸酶桥联酶标法快速检测呼吸道合胞病毒抗原

目的 探讨快速检测呼吸道合胞病毒抗原的方法。方法 应用碱性磷酸酶抗碱性磷酸酶桥联酶标法对１１４例临床诊断呼吸道病毒感染患儿鼻咽分泌牧标本进行呼吸道合胞病毒抗原的检测。结果：呼吸道合胞病毒抗原阳性者２２例，阳性率１９．３％。结论认为该检测方法快速简便，特异性高，敏感性好，适合临床实际应用。     

T淋巴细胞亚群与婴幼儿呼吸道合胞病毒感染关系的探讨

Ｔ淋巴细胞亚群与婴幼儿呼吸道合胞病毒感染关系的探讨甘子维，周惠敏，鱼绥和，王茂贵，陈明钰（唐都医院小儿科）关键词Ｔ淋巴细胞亚群，呼吸道合胞病毒，感染，婴幼儿中图法分类号Ｒ７２５．６婴幼儿呼吸道合胞病毒（ＲＳＶ）是婴幼儿急性下呼吸道感染的最重要病原之一...     

干扰素治疗50例呼吸道病毒感染疗效观察

呼吸道合胞病毒、流感病毒Ａ、Ｂ型、腺病毒、副流感病毒１，２，３型，是引起儿童上下呼吸道感染的常见病毒［１］。我国８０年代以来，呼吸道合胞病毒已成为呼吸道感染第一位病毒病原［２］。广州地区合胞病毒感染的流行季节主要发生在每年的春夏季，３月开始，４月达高...     

金莲清热泡腾片治疗小儿呼吸道合胞病毒肺炎的疗效观察

呼吸道合胞病毒肺炎(respiratory syncytial virus pneumonia)简称合胞病毒肺炎,是一种小儿常见的间质性肺炎,多发生于婴幼儿.我科于2009年8月开始在常规治疗基础上给予金莲清热泡腾片治疗小儿呼吸道合胞病毒肺炎,临床效果显著,现报告如下.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.