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Alexandre R. Espírito Santo John D. Bartlett Carolyn W. Gibson Yong Li Ashok B. Kulkarni Sérgio R. P. Line 《Connective tissue research》2013,54(1):39-52
Dental enamel is the most mineralized tissue of vertebrate organisms. Enamel biosynthesis is initiated by the secretion, processing, and self-assembly of a complex mixture of proteins. The formation of an ordered enamel organic extracellular matrix (ECM) seems be a crucial step for the proper formation of mineral phase. Polarizing microscopy demonstrates that the ordered supramolecular structure of the secretory-stage enamel organic ECM is strongly birefringent. In the present work we analyzed the birefringence of secretory-stage enamel organic ECM in amelogenin (Amelx)- and enamelysin (Mmp20)-deficient mice. Female Amelx+/? animals showed significant reduction in optical retardation values when compared with the Amelx+/+ subgroup (p = 0.0029). The secretory-stage enamel organic ECM of the Amelx?/? subgroup did not exhibit birefringence. The secretory-stage enamel organic ECM of Mmp20?/? mice showed a significant decrease in optical retardation as compared with Mmp20+/+ and Mmp20+/? mice (p = 0.0000). Mmp20+/? and Mmp20+/+ mice exhibited similar birefringence (p = 1.0000). The results presented here support growing evidence for the idea that the birefringence of secretory-stage enamel organic ECM is influenced by the ordered supramolecular organization of its components. 相似文献
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