白细胞介素2基因多态性与手足口病EV71脑炎患者的关联性分析

目的评价人类白细胞介素2-330T／G（IL-2—330T／G,rs2069762）基因多态性与肠道病毒EV71脑炎之间的关联。方法从5700例疑似手足LI病（HFMD）患儿中选择115名EV71型感染脑炎患儿和256名无合并症的EV71型HFMD患儿进行关联性分析。用荧光定量PCR进行Ev71型病毒的诊断。用高分辨熔解曲线技术分析和PCR—DNA测序法确定IL-2基因的多态性。结果在EV71型脑炎的患儿组中IL-2—330TT＋TG基因型的频率（93．91％）明显的高于EV71型感染的无合并症HFMD组（74．61％）,两者之间存在统计学差异（P=0．000）。IL-2．330T等位基因的频率在EV71型脑炎的患儿组（70．43％）明显的高于EV71型感染的无合并症HFMD组（51．95％）,是-个危险『生因素（OR=2．203,95％CI=0．127～0．756,P=0．001）。结论我们的研究结果表明,IL-2．330T等位基因是-个EV71型脑炎风险增加密切相关的因素。     

血清IL-10水平及其基因-1082A/G位点单核苷酸多态性与胃窦癌恶病质的关系

目的探讨胃窦癌病人血清细胞因子白细胞介素10（IL-10）水平及-1082A/G位点单核苷酸多态性与恶病质发生的关系。方法采用放射免疫学方法检测150例胃窦癌病人及135例健康人（对照组）血清IL-10水平。用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测胃窦癌病人IL-10基因-1082A/G位点单核苷酸多态性。结果胃窦癌病人血清IL-10水平较对照组显著升高（Z=-11.862,P〈0.001）,胃窦癌Ⅲ、Ⅳ期病人血清IL-10水平较Ⅰ、Ⅱ期显著升高（Z=-10.028,P〈0.001）。胃窦癌恶病质病人血清IL-10水平较非恶病质病人显著升高（Z=-10.369,P〈0.001）。Logistic回归分析显示,IL-10为恶病质发生的高风险性因素（OR=1.559,95%CI=1.299-1.870,P〈0.001）。单核苷酸多态性分析显示,胃窦癌恶病质病人IL-10基因-1082G等位基因频率较非恶病质病人显著升高（χ2=3.953,P〈0.05）。胃窦癌恶病质病人IL-10基因-1082AG基因型频率较非恶病质病人显著升高（χ2=4.511,P〈0.05）。Logistic回归分析显示,校正肿瘤分期后,IL-10基因-1082AG基因型为胃窦癌恶病质的高风险性因素（OR=2.295,95%CI=1.029-5.117,P〈0.05）。结论血清IL-10水平高及IL-10基因-1082AG基因型与胃窦癌病人恶病质的发生具有相关性。     

IFN-γ＋874位点单核苷酸多态性和蛋白表达水平与肠道病毒71感染关系

目的研究干扰素Ⅱ型（IFN-γ）基因＋874位点单核苷酸多态性及IFN-γ在血清中表达水平与肠道病毒71（EV71）感染的关系,探讨EV71感染的遗传易感因素。方法取急性期手足口病病儿粪便进行病毒分离,序列特异性引物-聚合酶链反应（PCR-SSP）技术检测EV71感染者及正常对照儿童IFN-γ基因第一内含子＋874位点T/A单核苷酸多态性;ELISA法测定血清中IFN-γ的表达水平。结果 78例手足口病病儿中有43例为EV71感染。EV71感染病儿血清中IFN-γ的水平显著高于正常对照组（t=6.477,P〈0.01）。EV71感染病儿IFN-γ基因型与对照组比较差异有显著性（2χ=6.149,P〈0.05）。EV71感染病儿等位基因频率与对照组比较差异有显著性（2χ=7.486,P〈0.05）。EV71一般感染组IFN-γ基因型分布与脑炎组相比,差异无显著意义（P〉0.05）,脑炎组IFN-γ等位基因A频率明显高于一般感染组（χ2=6.211,P〈0.05）。结论 IFN-γ表达水平和其基因第一内含子＋874位点多态性与EV71感染有关,与是否发生EV71脑炎有一定相关性。     

IL-10基因启动子区-592A/C位点多态性与HBV感染转归的关系

目的探讨汉族人白细胞介素-10(IL-10)基因启动子区-592位点单核苷酸多态性与乙型肝炎病毒(HBV)感染、转归的关系。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法,检测231例HBV感染者,165例HBV感染康复者和135例正常对照者IL-10基因启动子区-592A/C位点基因型。结果IL-10基因启动子区-592A/C位点基因型和等位基因在HBV感染组、HBV感染康复组和正常对照组之间的分布频率比较差异无显著性(P>0.05),在血清HBV-DHA<1×103copies/ml的HBV感染者组和HBV-DHA≥1×103copies/ml组之间的分布频率比较差异亦无显著性(P>0.05);但IL-10基因启动子区-592A/C位点AA基因型和A等位基因在慢性乙型肝炎组出现的频率明显高于HBV无症状携带组,两组之间分布比较差异有显著性(P<0.05)。结论汉族人IL-10基因启动子区-592A/C位点多态性可能与人群对HBV易感性及感染后的病毒血症水平无显著相关性,但该位点基因多态性与HBV感染后的肝脏炎症反应有关。     

IL-10基因单核苷酸多态性与急性淋巴细胞白血病易感性的相关性研究

目的 探讨IL-10基因单核苷酸多态性与ALL发病易感性的关系.方法 选取2007年1月至2009年12月北京大学第一医院、北京市道培医院115例ALL缓解患者,同时选取323名体检健康者作为对照组.采集ALL患者的骨髓以及健康对照者的外周血标本,提取DNA.设计IL-10启动子区-819C/T、-592A/C引物做PCR,应用限制性内切酶Msl Ⅰ、HpyCH4Ⅲ分析其限制性片段长度多态性,并测序验证;同时分析IL-10基因-819位点、-592位点各基因型构成及等位基因在ALL患者组和健康对照组间的差异.用实时定量PCR检测ALL患者EB病毒DNA和BCR/ABL融合基因,分析IL-10基因-819位点、-592位点各基因型构成及等位基因在EB病毒阳性和阴性组间、BCR/ABL融合基因阳性和阴性组间的差异.结果 ALL患者组IL-10基因-819位点的-819CC、-819TT、-819CT基因型比例分别为14.8%(17/115)、45.2%(52/115)、40.0%(46/115),-592位点的-592AA、-592CC、-592AC基因型比例分别为43.5%(50/115)、16.5%(19/115)、40.0%(46/115);健康对照组-819位点的-819CC、-819TT、-819CT基因型比例分别为9.9%(32/323)、16.4%(53/323)、73.7%(238/323),-592位点的-592AA、-592CC、-592AC基因型比例分别为11.8%(38/323)、15.5%(50/323)、72.8%(235/323),ALL患者组与健康对照组间-819和-592位点基因型构成差异均有统计学意义(x2值分别为46.000和54.550,P均＜0.05＝.ALL患者组-819T等位基因比例为65.2%(150/230),-592A等位基因比例为63.5%(146/230),而健康对照组分别为53.5%(344/646)和48.1%(311/646),差异均有统计学意义(x2值分别为9.877和15.986,P均＜0.05＝.ALL患者中42例检测了EB病毒DNA,其中EB病毒阳性22例,EB病毒阴性20例.EB病毒阳性组-819位点的-819CC、-819TT、-819CT基因型比例分别为9.1%(2/22)、40.9%(9/22)、50.0%(11/22),-592位点的-592AA、-592CC、-592AC基因型比例分别为31.8%(7/22)、13.6%(3/22)、54.5%(12/22),EB病毒阴性组分别为35.0%(7/20)、45.0%(9/20)、20.0%(4/20)和35.0%(7/20)、45.0%(9/20)、20.0%(4/20),2组基因型构成差异均无统计学意义(P均＞0.05).ALL患者中36例进行了BCR/ABL融合基因检测,其中阳性20例,阴性16例.BCR/ABL融合基因阳性组-819位点的-819CC、-819TT、-819CT基因型比例分别为0%(0/20)、45.0%(9/20)、55.0%(11/20),-592位点的-592AA、-592CC、-592AC基因型比例分别为45.0%(9/20)、5.0%(1/20)、50.0%(10/20),BCR/ABL融合基因阴性组分别为18.8%(3/16)、50.0%(8/16)、31.3%(5/16)和50.0%(8/16)、18.8%(3/16)、31.3%(5/16),2组基因型构成差异均无统计学意义(P均＞0.05).结论 IL-10基因-819位点TT基因型和-592位点AA基因型人群易患ALL.

Abstract：

Objective To observe the relationship of IL-10 gene single nucleotide polymorphism and the susceptibility to ALL. Methods The bone marrow and peripheral blood samples from 115 ALL patients and 323 healthy controls were collected in Peking University First Hospital and Beijing Dao-pei Hospital from January 2007 to December 2009. The DNA were extracted from all samples. The primers of -819C/T and -592A/C in the promoter region of IL-10 gene were designed for the PCR. The restrictive fragment length polymorphism of IL-10 gene was analyzed by using restrictive enzyme Msl Ⅰ and HpyCH4 Ⅲ.Sequencing was done in part of these samples to confirm the results of PCR. The differences of genotypes and allele ratio of -819 and -592 sites were analyzed between the ALL patients and healthy controls. Real-time quantitative PCR was performed to detect the EB virus (EBV) infection and the expression of BCR/ABL fusion gene. The differences of genotypes and allele ratio of -819 and -592 sites were analyzed between the positive and negative group. Results The genotype ratios of -819CC, -819TT, - 819CT, -592AA,- 592CC and - 592AC were 14. 8% ( 17/115 ), 45.2% ( 52/115 ), 40. 0% ( 46/115 ), 43.5% ( 50/115 ),16. 5% ( 19/115 ), 40. 0% ( 46/115 ) in ALL patients, and were 9. 9% ( 32/323 ), 16. 4% ( 53/323 ),73.7% ( 238/323 ), 11.8% ( 38/323 ), 15.5% ( 50/323 ), 72. 8% ( 235/323 ) in the healthy controls,respectively. The genotypes of -819 and -592 sites had statistically significant differences between the two groups(x2 values were 46.000 and 54.550, all P ＜ 0. 05 ). The allele ratio of -819T and -592A were (65.2%, 150/230) and (63.5%, 146/230) in ALL patients, while they were 53.5% (344/646) and 48. 1% (311/646)in the healthy controls. There were statistically significant differences between the two groups (x2 values were 9. 877 and 15.986, all P ＜ 0. 05 ). The EBV DNA were detected in 42 ALL patients,among which 22 were positive and 20 were negative. The genotype ratios of -819CC, -819TT, -819CT,-592AA, - 592CC, - 592AC in EBV positive group were 9. 1% ( 2/22 ), 40. 9% ( 9/22 ), 50. 0%(11/22) ,31.8% ( 7/22 ), 13.6% ( 3/22 ), 54. 5% ( 12/22 ), while they were 35.0% ( 7/20 ), 45.0%(9/20) ,20. 0% (4/20) ,35.0% (7/20) ,45.0% (9/20) ,20. 0% (4/20) in the EBV negative group. The genotypes of -819 and -592 sites showed no statistical differences between the two groups( all P ＞ 0. 05 ).The BCR/ABL fusion gene were detected in 36 ALL patients, among which 20 were positive and 16 were negative. The genotype ratios of - 819CC, - 819TT, - 819CT, - 592AA, - 592CC, - 592AC in BCR/ABL positive group were 0% (0/20) ,45.0% (9/20) ,55.0% ( 11/20), 45. 0% (9/20) ,5.0% (1/20) ,50. 0%( 10/20), while they were 18. 8% ( 3/16 ), 50. 0% ( 8/16), 31.3% ( 5/16 ), 50. 0% ( 8/16 ), 18. 8%(3/16), 31.3 % (5/16)in the BCR/ABL negative group. The genotypes of -819 and -592 sites showed no statistical differences between the two groups ( all P ＞ 0. 05 ). Conclusion The population with - 819TT and - 592AA genotype of IL-10 gene shows susceptibility to ALL.     

IL-10基因单核苷酸多态性与急性淋巴细胞白血病易感性的相关性研究

Objective To observe the relationship of IL-10 gene single nucleotide polymorphism and the susceptibility to ALL. Methods The bone marrow and peripheral blood samples from 115 ALL patients and 323 healthy controls were collected in Peking University First Hospital and Beijing Dao-pei Hospital from January 2007 to December 2009. The DNA were extracted from all samples. The primers of -819C/T and -592A/C in the promoter region of IL-10 gene were designed for the PCR. The restrictive fragment length polymorphism of IL-10 gene was analyzed by using restrictive enzyme Msl Ⅰ and HpyCH4 Ⅲ.Sequencing was done in part of these samples to confirm the results of PCR. The differences of genotypes and allele ratio of -819 and -592 sites were analyzed between the ALL patients and healthy controls. Real-time quantitative PCR was performed to detect the EB virus (EBV) infection and the expression of BCR/ABL fusion gene. The differences of genotypes and allele ratio of -819 and -592 sites were analyzed between the positive and negative group. Results The genotype ratios of -819CC, -819TT, - 819CT, -592AA,- 592CC and - 592AC were 14. 8% ( 17/115 ), 45.2% ( 52/115 ), 40. 0% ( 46/115 ), 43.5% ( 50/115 ),16. 5% ( 19/115 ), 40. 0% ( 46/115 ) in ALL patients, and were 9. 9% ( 32/323 ), 16. 4% ( 53/323 ),73.7% ( 238/323 ), 11.8% ( 38/323 ), 15.5% ( 50/323 ), 72. 8% ( 235/323 ) in the healthy controls,respectively. The genotypes of -819 and -592 sites had statistically significant differences between the two groups(x2 values were 46.000 and 54.550, all P ＜ 0. 05 ). The allele ratio of -819T and -592A were (65.2%, 150/230) and (63.5%, 146/230) in ALL patients, while they were 53.5% (344/646) and 48. 1% (311/646)in the healthy controls. There were statistically significant differences between the two groups (x2 values were 9. 877 and 15.986, all P ＜ 0. 05 ). The EBV DNA were detected in 42 ALL patients,among which 22 were positive and 20 were negative. The genotype ratios of -819CC, -819TT, -819CT,-592AA, - 592CC, - 592AC in EBV positive group were 9. 1% ( 2/22 ), 40. 9% ( 9/22 ), 50. 0%(11/22) ,31.8% ( 7/22 ), 13.6% ( 3/22 ), 54. 5% ( 12/22 ), while they were 35.0% ( 7/20 ), 45.0%(9/20) ,20. 0% (4/20) ,35.0% (7/20) ,45.0% (9/20) ,20. 0% (4/20) in the EBV negative group. The genotypes of -819 and -592 sites showed no statistical differences between the two groups( all P ＞ 0. 05 ).The BCR/ABL fusion gene were detected in 36 ALL patients, among which 20 were positive and 16 were negative. The genotype ratios of - 819CC, - 819TT, - 819CT, - 592AA, - 592CC, - 592AC in BCR/ABL positive group were 0% (0/20) ,45.0% (9/20) ,55.0% ( 11/20), 45. 0% (9/20) ,5.0% (1/20) ,50. 0%( 10/20), while they were 18. 8% ( 3/16 ), 50. 0% ( 8/16), 31.3% ( 5/16 ), 50. 0% ( 8/16 ), 18. 8%(3/16), 31.3 % (5/16)in the BCR/ABL negative group. The genotypes of -819 and -592 sites showed no statistical differences between the two groups ( all P ＞ 0. 05 ). Conclusion The population with - 819TT and - 592AA genotype of IL-10 gene shows susceptibility to ALL.     

IL-10基因单核苷酸多态性与急性淋巴细胞白血病易感性的相关性研究

Objective To observe the relationship of IL-10 gene single nucleotide polymorphism and the susceptibility to ALL. Methods The bone marrow and peripheral blood samples from 115 ALL patients and 323 healthy controls were collected in Peking University First Hospital and Beijing Dao-pei Hospital from January 2007 to December 2009. The DNA were extracted from all samples. The primers of -819C/T and -592A/C in the promoter region of IL-10 gene were designed for the PCR. The restrictive fragment length polymorphism of IL-10 gene was analyzed by using restrictive enzyme Msl Ⅰ and HpyCH4 Ⅲ.Sequencing was done in part of these samples to confirm the results of PCR. The differences of genotypes and allele ratio of -819 and -592 sites were analyzed between the ALL patients and healthy controls. Real-time quantitative PCR was performed to detect the EB virus (EBV) infection and the expression of BCR/ABL fusion gene. The differences of genotypes and allele ratio of -819 and -592 sites were analyzed between the positive and negative group. Results The genotype ratios of -819CC, -819TT, - 819CT, -592AA,- 592CC and - 592AC were 14. 8% ( 17/115 ), 45.2% ( 52/115 ), 40. 0% ( 46/115 ), 43.5% ( 50/115 ),16. 5% ( 19/115 ), 40. 0% ( 46/115 ) in ALL patients, and were 9. 9% ( 32/323 ), 16. 4% ( 53/323 ),73.7% ( 238/323 ), 11.8% ( 38/323 ), 15.5% ( 50/323 ), 72. 8% ( 235/323 ) in the healthy controls,respectively. The genotypes of -819 and -592 sites had statistically significant differences between the two groups(x2 values were 46.000 and 54.550, all P ＜ 0. 05 ). The allele ratio of -819T and -592A were (65.2%, 150/230) and (63.5%, 146/230) in ALL patients, while they were 53.5% (344/646) and 48. 1% (311/646)in the healthy controls. There were statistically significant differences between the two groups (x2 values were 9. 877 and 15.986, all P ＜ 0. 05 ). The EBV DNA were detected in 42 ALL patients,among which 22 were positive and 20 were negative. The genotype ratios of -819CC, -819TT, -819CT,-592AA, - 592CC, - 592AC in EBV positive group were 9. 1% ( 2/22 ), 40. 9% ( 9/22 ), 50. 0%(11/22) ,31.8% ( 7/22 ), 13.6% ( 3/22 ), 54. 5% ( 12/22 ), while they were 35.0% ( 7/20 ), 45.0%(9/20) ,20. 0% (4/20) ,35.0% (7/20) ,45.0% (9/20) ,20. 0% (4/20) in the EBV negative group. The genotypes of -819 and -592 sites showed no statistical differences between the two groups( all P ＞ 0. 05 ).The BCR/ABL fusion gene were detected in 36 ALL patients, among which 20 were positive and 16 were negative. The genotype ratios of - 819CC, - 819TT, - 819CT, - 592AA, - 592CC, - 592AC in BCR/ABL positive group were 0% (0/20) ,45.0% (9/20) ,55.0% ( 11/20), 45. 0% (9/20) ,5.0% (1/20) ,50. 0%( 10/20), while they were 18. 8% ( 3/16 ), 50. 0% ( 8/16), 31.3% ( 5/16 ), 50. 0% ( 8/16 ), 18. 8%(3/16), 31.3 % (5/16)in the BCR/ABL negative group. The genotypes of -819 and -592 sites showed no statistical differences between the two groups ( all P ＞ 0. 05 ). Conclusion The population with - 819TT and - 592AA genotype of IL-10 gene shows susceptibility to ALL.     

IL-10基因启动子-592A/C多态性与HBV感染预后的关系

摘 要 目的：探讨湖北汉族人白细胞介素-10（IL-10）基因启动子区-592位点单核苷酸多态性与乙型肝炎病毒（HBV）感染、转归的关系。方法：采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法，检测231例慢性HBV感染者（其中慢性乙型肝炎75例，无症状携带156例），165例HBV感染自愈者和135例正常对照者IL-10基因启动子区-592A/C位点基因型。结果：IL-10基因启动子区-592A/C位点基因型和等位基因在慢性HBV感染组、HBV感染自愈组和正常对照组之间的分布频率比较差异无显著性（P>0.05），在血清HBV-DNA<1×103copies/mL的慢性HBV感染者组和HBV-DNA≥1×103 copies/mL组之间的分布频率比较差异亦无显著性（P>0.05）；但IL-10基因启动子区-592位点AA基因型和A等位基因在慢性乙型肝炎组出现的频率明显高于HBV无症状携带组，两组之间分布比较差异有显著性（P<0.05）。结论：汉族人IL-10基因启动子区-592位点多态性可能与人群对HBV易感性及感染后的病毒血症水平无显著相关性，但该位点基因多态性与HBV感染后的肝脏炎症反应有关。≥≥≥≥≥     

慢性HBV感染者IL-10启动子592位点基因多态性与血清IL-10的表达分析

目的探讨慢性乙型肝炎患者IL-10-592各基因型与血清中IL-10表达水平的关系。方法以117例HBV感染者作为疾病组,81例体检健康者(仅抗HBc为阳性的既往感染者)作为对照组。用聚合酶链反应-限制性片段长度多态性(PCRRFLP)技术检测两组IL-10-592基因型,ELISA法检测两组血清IL-10的浓度。结果疾病组血清IL-10水平为348.28(231.9,411.76)pg/mL,与对照组的228.74(160.59,364.18)pg/mL比较,差异有统计学意义(H=14.49,P0.05)。患者IL-10-592 CC基因型血清IL-10水平为448.64(402.51,584.78)pg/mL,与AA基因型294.78(190.49,354.36)pg/mL和AC基因型297.01(205.99,385.33)pg/mL比较,差异均有统计学意义(H分别为37.87和30.92,P均0.05)。而AA基因型与AC基因型比较,差异无统计学意义(H=0.10,P0.05)。结论慢性乙肝患者IL-10启动子592位点的多态性影响血清IL-10的水平,CC基因型促进IL-10高表达,AA基因型引起IL-10低表达。     

白细胞介素-1F7基因rs3811047位点单核苷酸多态性对类风湿关节炎易感性与病情的影响

目的 探讨白细胞介素-1(IL-1)F7 基因中rs3811047 位点单核苷酸多态性(SNP)对类风湿关节炎(RA)易感性和病情的影响.方法 收集RA 患者184 例和同期正常健康献血者184 人为对照组,采用连接酶检测反应(LDR)PCR 方法 检测IL-1 F7 基因rs3811047 位点SNP,比较其等位基因频率及基因型频率在两组中的分布,并比较不同基因型RA 患者临床表现的差别.结果 RA 组和对照组IL-1 F7 基因rs3811047 位点A 等位基因频率分别为(16.21%,17.77%),G 等位基因频率为分别为(83.79%,82.32%),差异无统计学意义(P ＞0.05);AA、AG、GG 基因型分布频率在RA 组为2.20%、28.02%、69.78%,对照组为2.76%、29.83%、67.40%,两组间差异亦无统计学意义(P ＞ 0.05).184 例RA 患者中,含有A 等位基因的患者(AA 和AG 基因型)55 例,其关节肿胀数为(4.60 ±5.52)个、关节肿胀指数为4.89 ±5.70、休息痛为3.57 ±2.46、日常生活能力(HAQ)评分为(0.67 ± 0.70)分,不含A 等位基因的患者(GG 基因型)127 例,上述4 个指标的水平分别为(6.64 ±5.46)个、7.14 ±5.71、4.52 ±2.39、(0.99 ±0.67)分,两组间的差异均有统计学意义(P ＜0.05).结论 安徽籍汉族人群RA 易感性与IL-1 F7 基因中rs3811047 位点SNP 无关;但含有A 等位基因的RA 患者病情好于不含有等位基因A 的RA 患者,提示IL-1 F7 基因rs3811047 位点A 等位基因对RA 患者病情可能有一定的保护作用.     

肠道病毒中枢神经系统感染病儿免疫功能紊乱与神经元损害的相关性

目的通过检测肠道病毒（EV）中枢神经系统感染病儿脑脊液中白细胞介素-10（IL-10）、γ-干扰素（IFN-γ）及神经元特异性烯醇化酶（NSE）的水平,探讨EV引起病儿免疫功能紊乱的机制。方法收集2008年1月—2009年1月青岛大学医学院附属医院和滨州医学院附属医院临床诊断为无菌性脑膜炎、脑炎病儿的急性期脑脊液标本100例。采用逆转录聚合酶链反应（RT-PCR）方法,检测标本中的EV RNA;应用双抗体夹心ELISA法检测EV感染组和对照组脑脊液中IL-10、IFN-γ及NSE的表达水平。结果 100例急性期病儿脑脊液中,经RT-PCR和二次PCR共获得阳性结果78例（78.0%）;EV感染组IFN-γ的表达水平高于对照组（t=4.24,P〈0.01）,重症组高于轻症组（t=4.13,P〈0.01）;IL-10表达水平低于对照组（t=7.89,P〈0.01）,重症组低于轻症组（t=2.97,P〈0.01）;NSE表达水平高于对照组（t=7.02,P〈0.01）,重症组高于轻症组（t=2.79,P〈0.01）。EV感染组NSE与IFN-γ水平呈正相关（r=0.673,P〈0.05）,与IL-10水平呈负相关（r=-0.587,P〈0.05）。结论 IL-10和IFN-γ参与EV对中枢神经系统免疫损害的过程,并与神经元的损害程度密切相关。     

胃癌病人外周血XPD751基因多态性与奥沙利铂化疗敏感性的关系

目的探讨胃癌病人外周血白细胞中DNA修复基因XPD751基因多态性与奥沙利铂化疗效果的关系。方法收集经病理学检查确诊的胃癌128例,所有病例化疗前取静脉血,提取白细胞DNA,用TaqMan-MGB探针等位基因分型技术检测XPD751基因型。比较其基因型与接受奥沙利铂化疗病人临床疗效的关系。结果在128例胃癌病人中,XPD751A／A、A／C和C／C基因型分别为104（81．3％）、23（17．9％）和1例（0．8％）。经化疗后,63例病人有效,总有效率为49．2％。XPD751A／A、A／C＋C／C基因型有效率分别为53．3％（56／105）、30．4％（7／23）,两者相比差异有统计学意义（χ^2=3．958,P（O．05）。携带A／A基因型病人的腹泻程度高于A／C＋C／C基因型病人（χ^2=7．212,P〈O．05）。结论XPD751基因多态性可能与胃癌对奥沙利铂为基础的化疗敏感性相关。     

MMIF—rs755622位点单核苷酸基因多态性与子痫前期发病关系

目的研究巨噬细胞移动抑制因子（MMIF）-rs755622位点单核苷酸基因多态性与子痫前期发病的关系。方法选取子痫前期孕妇165例,分为轻度（72例）和重度子痫前期组（93例）。另选同期正常孕妇120例为对照组。采用聚合酶链限制性片段长度多态性（PCR—RFLP）方法对该位点进行基因分型,比较各组基因型频率和等位基因频率的差异。测定各组空腹血糖（FBG）、空腹胰岛素（FIN）水平,计算稳态模型胰岛素抵抗指数（HOMA—IR）;比较不同基因型在各组内FBG、FIN以及HOMA-IR的差异。结果MMIF-rs755622位点共检出GG、GC和CC等3种基因型。子痫前期组与对照组比较,基因型频率与等位基因频率差异无显著性（P〉0．05）。轻度、重度子痫前期组间基因型频率与等位基因频率比较差异也无显著性（P〉0．05）。轻度子痫前期组与重度子痫前期组内GC和CC基因型孕妇FIN及HOMA—IR均高于GG基因型孕妇,差异均有显著性（t=3．17～9．74,P〈0．05）。结论MMIF—rs755622位点单核苷酸基因多态性与子痫前期IR密切相关,CG和CC基因型加重了子痫前期IR的程度,但该位点可能不是中国汉族子痫前期孕妇发病的易感位点     

谷胱甘肽转硫酶M_1和T_1多态性与大肠癌易患性关系研究

目的：了解江苏部分地区人群中谷胱甘肽转硫酶ＧＳＴＭ_１和ＧＳＴＴ_１基因多态性与大肠癌易患性的关系。方法：应用多重ＰＣＲ法．对照研究５５例大肠癌患者和６２例健康人的ＧＳＴＭ_１和ＧＳＴＴ_１基因频率。结果：在健康对照和大肠癌病例中,ＧＳＴＭ_１基因缺失频率分别为５３．２％和６１．８％,ＧＳＴＴ_１基因缺失频率分别为５０．０％和５６．４％,病例组基因缺失频率高于健康对照组,但无显著性差异（Ｐ＞０．０５）。结论：ＧＳＴＭ_１和ＧＳＴＴ_１基因缺失与大肠癌易患性和发病年龄无明显相关性。     

胃癌病人ERCC1和XPD基因多态性与奥沙利铂化疗预后关系

目的了解DNA损伤修复基因ERCC1 Asn118Asn和XPD Lys751Gln多态性与接受以奥沙利铂为基础化疗的晚期胃癌病人预后的关系。方法 90例晚期胃癌病人接受奥沙利铂化疗前抽取静脉血并提取DNA,应用real-ti me PCR法进行基因分型。比较病人不同基因型与生存期的关系。结果晚期胃癌病人ERCC1Asn118Asn基因型频率为C/C 47.78%,C/T42.22%,T/T10.00%;XPD基因型频率分别为A/A80.00%,A/C16.67%,C/C 3.33%。90例胃癌病人中位无疾病进展生存期（TTP）6.4个月,总生存期（OS）9.5个月。ERCC1C/C基因型病人中位TTP为6.6个月,OS为9.7个月;C/T＋T/T型病人中位TTP为6.1个月,OS为9.1个月,二者差异无统计学意义（P〉0.05）。XPD A/A基因型病人中位TTP为6.7个月,OS为9.7个月;A/C＋C/C基因型病人中位TTP为4.8个月,OS为7.8个月,二者差异有统计学意义（χ2=20.244、17.113,P〈0.05）。同时携带ERCC1 C/C及XPD A/A基因型、ERCC1 C/C及XPD A/C＋C/C基因型、ERCC1 C/T＋T/T及XPD A/A基因型、ERCC1 C/T＋T/T及XPD A/C＋C/C基因型的病人中位TTP分别为6.9、4.7、6.3和5.0个月,OS为9.9、7.7、9.3、8.0个月,4组差异有统计学意义（χ2=18.331、12.742,P〈0.05）。结论 XPD Lys751Gln基因多态性与以接受奥沙利铂为基础化疗的晚期胃癌病人的生存期有关,ERCC1 Asn118Asn基因多态性与接受奥沙利铂为基础化疗的晚期胃癌病人的生存期无关。     

XPGC46T单核苷酸多态性与晚期结直肠癌对奥沙利铂化疗敏感性的关系

目的探讨人着色性干皮病G组（XPG）C46T基因多态性与以奥沙利铂为主的晚期结直肠癌化疗敏感性的关系。方法经病理检查确诊的Ⅳ期结直肠癌病人73例,治疗前提取外周血DNA,应用TaqMan-MGB探针等位基因分型技术进行基因分型,比较其基因型与接受奥沙利铂化疗病人的2周期临床疗效、无进展生存期（PFS）的关系。结果C/C野生基因型病人的近期有效率（73.0%）明显高于C/T＋T/T突变基因型的病人（P=0.014）;C/C基因型病人PFS（中位时间为11个月）明显长于C/T＋T/T基因型病人（6个月）,差异具有显著性（χ^2=28.90,P〈0.01）。结论XPGC46T基因多态性可能与以奥沙利铂化疗的晚期结直肠癌病人化疗敏感性和生存时间相关。     

HPV16 E6和E7基因序列多态性与宫颈癌临床病理特征相关性

目的探讨宫颈癌人乳头瘤病毒16型（HPV16）E6和E7转化基因序列多态性,及其与宫颈癌临床病理特征的相关性。方法从77例宫颈癌组织中筛选出51例HPV16阳性标本,扩增E6及E7基因,PCR产物进行序列测定。应用DNAStar生物软件进行核苷酸和氨基酸序列的分析。结果宫颈癌组织中HPV16 E6共测得6个突变位点,均为错义突变;最常见的突变位点为T178G/A（D29E）,突变频率为56.00%,T178G/A突变在不同分化程度、不同分期、不同肿瘤大小及有无淋巴结转移组的分布频率差异无显著性（P〉0.05）。宫颈癌组织中HPV16 E7共测得4个突变位点,2个为错义突变;最常见的突变位点为A647G（N29S）,突变频率为68.09%,A647G在中分化及低分化组的突变频率分别为56.67%、88.24%,两组间突变频率差异有显著性（χ2=4.98,P〈0.05）;A647G在不同分期、不同肿瘤大小、有无淋巴结转移组的分布频率差异无显著性（P〉0.05）。结论青岛地区HPV16 E6基因T178G/A突变与宫颈癌的发生发展无关,HPV16 E7基因A647G突变与宫颈癌的分化程度呈负相关,可以作为评估宫颈癌恶性程度的指标。     

消化性溃疡患者HP感染与IL—6,IL—8活性的关系

该文研究消化性溃疡患者ＨＰ感染与ＩＬ－６、ＩＬ－８细胞因子的活性及炎症反应的关系。方法；胃镜下，在溃疡周边取胃粘膜组织进行ＨＰ尿素酶反应、病理检查和制备胃粘膜培养液、并用ＥＬＩＳＡ夹心法检测ＩＬ－６、ＩＬ－８活性。结果：消化性溃疡患者ＨＰ感染占８８％，无感染的溃疡患者占１２．５，ＨＰ感染占３８％；ＨＰ感染肝粘膜ＩＬ－６、ＩＬ－８活性均显著高于对照组和ＨＰ阴性组；ＨＰ根除后胃粘膜ＩＬ－６、ＩＬ－８     

THE MAMMALIAN CELL-VIRUS RELATIONSHIP : V. SUSCEPTIBILITY AND RESISTANCE OF CELLS IN VITRO TO INFECTION BY COXSACKIE A9 VIRUS

All primary cell cultures tested from numerous human and rhesus monkey tissues were susceptible to Coxsackie A9 virus infection and were found to contain a specific A9 virus receptor. Ability of these cells to adsorb actively virus correlated with the presence of this receptor. Attachment of A9 virus to cell receptor was specifically enhanced by calcium and magnesium ions. Human cells established in continuous culture from pretested susceptible cultures were found to have lost A9 virus susceptibility and A9 receptors but not receptors for adsorption of type 1 poliovirus or B1, B3, B5 Coxsackie viruses. These cultures were able also to produce infectious A9 virus after exposure to viral RNA. Coxsackie A9 virus as well as type 1 poliovirus, inactivated following reaction with debris prepared from susceptible cells, could be recovered fully in infectious form by treatment of the inactive systems at low pH. Recovery of infectious A9 virus was also possible with a chelating compound. Coxsackie A9 virus could also be dissociated by means of low pH after adsorption onto susceptible cells for 2 minutes at 1°C., but not after 1 hour incubation at 37°C., indicating that viral host cell activity may be required in order for irreversible enterovirus attachment to take place.