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HPLC法测定交泰片中盐酸小檗碱的含量 总被引:1,自引:1,他引:0
目的:采用高效液相测定交泰片中盐酸小檗碱的含量。方法:采用Hypersil C18色谱枉(250mm×4.6mm,10μm),以乙腈-0.1%磷酸(含磷酸二氢钾0.2%、十二烷基硫酸钠0.04%)(45:55)为流动相,检测波长345nm,流速1.0ml·min^-1。结果:盐酸小檗碱在1.01~30.30μg范围内线性关系良好,r=0.9999;平均回收率为99.6%(n=6),RSD=0.88%。结论:该方法准确,简便,快速,为交泰片中盐酸小檗碱建立了质量控制方法。 相似文献
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高效液相色谱法测定双黄消炎片中黄芩苷和盐酸小檗碱的含量 总被引:1,自引:3,他引:1
目的 建立高效液相色谱法同时测定双黄消炎片中黄芩苷和盐酸小檗碱含量的方法。方法采用ODS C18(200mm×4.6mm,5μm)色谱柱,流动相为乙腈0.05mol·L^-1磷酸二氢钾溶液三乙胺(25:75:0.05),流速为1.0mL·min^-1,检测波长为265nm。结果黄芩苷和盐酸小檗碱分别在19.0-380.0μg·mL^-1(r=0.9999)、1.01-20.18μg·mL^-1(r=0.9999)内线性关系良好,平均加样回收率(n=6)分别为100.1%和98.5%。结论该方法简便、准确、重复性好,可同时测定双黄消炎片中黄芩苷和盐酸小檗碱的含量。 相似文献
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高效液相色谱法测定痔疮片中盐酸小檗碱含量 总被引:1,自引:0,他引:1
目的:建立高效液相色谱法(HPLC法)以测定痔疮片中盐酸小檗碱的含量。方法:采用Alltima C18柱,以0.033mol/L的磷酸二氢钠溶液-乙腈(70:30)为流动相,流速为1.0mL/min,检测波长为265nm。结果:痔疮片中盐酸小檗碱线性范围是84.24—421.2ng,r=0.9999;平均回收率为98.72%,RSD为0.76%(n=6)。结论:HPLC法准确、重现性好,可作为该制剂的质量控制方法。 相似文献
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目的建立测定消渴平胶囊中盐酸小檗碱含量的高效液相色谱法。方法色谱枉为InertsilC18枉(250mm×4.6mm,5μm),流动相为0.1%磷酸溶液(每100mL加十二烷基磺酸钠0.1g)-乙腈(52:48),检测波长为265nm。结果盐酸小檗碱进样量线性范围为0.03095~0.07225μg,r=0.9999;平均加样回收率为100.60%,RSD=0.93%(n=6)。结论该方法线性关系良好、简便、准确、专属性强,可作为控制消渴平胶囊原药材和成品质量的方法。 相似文献
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目的建立小儿肠胃康颗粒盐酸小檗碱含量的测定方法。方法采用反相Prodigy ODS3色谱柱(250mm×4.6mm,5μm),柱温为35℃,流动相为乙腈-水(含0.4%磷酸,0.7%三乙胺)溶液(25:75),测定波长为350nm,以外标法定量。结果盐酸小檗碱质量浓度在4.01~200.5μg/mL范围内与峰面积线性关系良好(r=0.9999),平均回收率为98.45%,RSD=0.81%(n=6)。结论用高效液相色谱法测定小儿肠胃康颗粒中盐酸小檗碱的含量,操作简便,结果准确。 相似文献
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HPLC法测定盐酸黄连素溶液中盐酸小檗碱含量 总被引:1,自引:0,他引:1
目的:建立HPLC法测定盐酸黄连素溶液中盐酸小檗碱含量。方法:使用Hypersil ODS2 C18(200mm×4.6mm,5μm);乙腈:磷酸盐缓冲液[0.05mol·L^-1磷酸二氢钾和0.05mol·L^-1庚烷磺酸钠(1:1)](50:50)为流动相,流速1ml·min^-1,检测波长263nm。结果:盐酸小檗碱在0.848~4.240μg进样范围内线性关系良好(r=0.9999),平均回收率99.0%(RSD=1.11%)。结论:本法快速、简便、准确。 相似文献
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泌感片的薄层鉴别和盐酸小檗碱的含量测定 总被引:1,自引:1,他引:0
目的应用薄层色谱法对黄柏、甘草、柴胡、续断进行鉴别和高效液相色谱法测定盐酸小檗碱的含量。方法采用薄层色谱鉴别黄柏、甘草、柴胡、续断及高效液相色谱测定盐酸小檗碱含量,Shim—pack—C18柱(5μm,250mm×4.6mm),乙腈0.05mol·L^-1-磷酸二氢钠溶液(28:72)为流动相,检测波长347nm。结果薄层斑点清晰,含量测定线性范围为9.95~99.5mg·L^-1;r=1.0000,回收率为99.42%,RSD=1.21%。结论薄层色谱可用于黄柏、甘草、柴胡、续断的定性鉴别,且高效液相色谱法测定泌感片中盐酸小檗碱的含量具有操作简便、结果准确、灵敏的特点,可用于该制剂的质量控制。 相似文献
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Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.
Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.相似文献
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Petrikovics I McGuinn WD Sylvester D Yuzapavik P Jiang J Way JL Papahadjopoulos D Hong K Yin R Cheng TC DeFrank JJ 《Drug delivery》2000,7(2):83-89
This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine. 相似文献
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《Critical reviews in toxicology》2013,43(5-6):327-369
AbstractThe uptake of metals from food and water sources by insects is thought to be additive. For a given metal, the proportions taken up from water and food will depend both on the bioavailable concentration of the metal associated with each source and the mechanism and rate by which the metal enters the insect. Attempts to correlate insect trace metal concentrations with the trophic level of insects should be made with a knowledge of the feeding relationships of the individual taxa concerned. Pathways for the uptake of essential metals, such as copper and zinc, exist at the cellular level, and other nonessential metals, such as cadmium, also appear to enter via these routes. Within cells, trace metals can be bound to proteins or stored in granules. The internal distribution of metals among body tissues is very heterogeneous, and distribution patterns tend to be both metal and taxon specific. Trace metals associated with insects can be both bound on the surface of their chitinous exoskeleton and incorporated into body tissues. The quantities of trace meals accumulated by an individual reflect the net balance between the rate of metal influx from both dissolved and particulate sources and the rate of metal efflux from the organism. The toxicity of metals has been demonstrated at all levels of biological organization: cell, tissue, individual, population, and community. Much of the literature pertaining to the toxic effects of metals on aquatic insects is based on laboratory observations and, as such, it is difficult to extrapolate the data to insects in nature. The few experimental studies in nature suggest that trace metal contaminants can affect both the distribution and the abundance of aquatic insects. Insects have a largely unexploited potential as biomonitors of metal contamination in nature. A better understanding of the physico-chemical and biological mechanisms mediating trace metal bioavailability and exchange will facilitate the development of general predictive models relating trace metal concentrations in insects to those in their environment. Such models will facilitate the use of insects as contaminant biomonitors. 相似文献
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《Expert opinion on investigational drugs》2013,22(1):79-86
Advances in the molecular biological knowledge of neuronal nicotinic acetylcholine receptors (nAChRs) have led to a growing interest by the pharmaceutical industry in the development of novel compounds that selectively modulate nAChR function. The ability of (-)-nicotine, an activator of nAChRs, to enhance attentional aspects of cognition in animals and humans, to exert neuroprotective and anxiolytic-like effects, and presumably to mediate the negative correlation between smoking and Alzheimer's (and Parkinson's) Disease, has focused interest on the potential therapeutic utility of modulators of nAChR function for treatment of some of the deficits associated with these progressive, neurodegenerative conditions. Numerous compounds are known which activate nAChRs and which might serve as lead compounds toward the development of such agents. The pharmacologic diversity of neuronal nAChR subtypes suggests the possibility of developing selective compounds which would have more favourable side-effect profiles than existing agents. This broader class of agents, collectively called cholinergic channel modulators (ChCMs), is anticipated to encompass compounds which would have more favourable side-effect profiles than existing agents, which generally exhibit low selectivity. This selectivity may be achieved by preferentially activating some subtypes of nAChRs (i.e., Cholinergic Channel Activators, ChCAs) or inhibiting the function of other subtypes (Cholinergic Channel Inhibitors, ChCIs). An overview of the biology of nAChRs and the rationale for the use of ChCMs for the treatment of dementia related to neurodegenerative diseases are presented, followed by a discussion of lead compounds and compounds under consideration for clinical evaluation. 相似文献
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Chang Min Kim Kun Ho Son Sung Hwan Kim Hyun Pyo Kim 《Archives of pharmacal research》1991,14(4):305-310
In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins
from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins. 相似文献
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Alberto García-Lled Javier Gmez-Pavn Juan Gonzlez del Castillo Teresa Hernndez-Sampelayo Mari Cruz Martín-Delgado Francisco Javier Martín Snchez Manuel Martínez-Sells Jos María Molero García Santiago Moreno Guilln Fernando Rodríguez-Artalejo Julin Ruiz-Galiana Rafael Cantn Pilar De Lucas Ramos Alejandra García-Botella Emilio Bouza 《Rev Esp Quimioter》2022,35(2):115
The precocity and efficacy of the vaccines developed so far against COVID-19 has been the most significant and saving advance against the pandemic. The development of vaccines has not prevented, during the whole period of the pandemic, the constant search for therapeutic medicines, both among existing drugs with different indications and in the development of new drugs. The Scientific Committee of the COVID-19 of the Illustrious College of Physicians of Madrid wanted to offer an early, simplified and critical approach to these new drugs, to new developments in immunotherapy and to what has been learned from the immune response modulators already known and which have proven effective against the virus, in order to help understand the current situation. 相似文献