1,6—二磷酸果糖对组胺升高豚鼠气道阻力的抑制作用

为了探讨１，６－二磷酸果糖的平喘作用，我们比较了研究了ＦＤＰ对组胺致敏豚鼠呼吸力学的影响。结果表明，给豚鼠静注组胺８μｇ／ｋｇ３０ｓ后，肺阻力为（０．０３２±０．００４）ｋＰａ；ｍｌ＾－１．ｓ＾－１升至（０．０８８±０．０１４）ｋＰａ．ｍｌ＾－１．ｓ＾－１。而静注ＦＤＰ４００，ｇ／ｋｇ１５ｍｉｎ后再次静注同等剂量组胺，在３０ｓ肺阻力仅从（０．０３２±０．００６）ｋＰａ．ｍｌ＾－１．ｓ＾－１，升至（     

依那普利对高血压左心室肥厚患者血浆P物质和肾素—血管紧张素…

观察２７例高血压病伴左心室肥厚患依那普利治疗１２周前后左心室质量指数，血浆肾素活性及血浆Ｐ物质血管紧张素Ⅱ和醛固酮水平的变化。结果：血浆ＳＰ水平由治疗前（１２．６２±５．１１）ｎｇ．Ｌ＾－１升高至（３７．２１±１１．０６）ｎｇ．１０＾－１ＬＶＭＩ由治疗前（１３９．２９±１７．９８）ｇ．ｍ＾－２降低（１０７．７７±１０．９４）ｇ．ｍ＾－２；ＰＲＡ由治疗前（２．３３±５３．３９）μｇ．Ｌ＾－１．ｈ＾     

前胡丙素对肾血管性高血压大鼠脑肾ATP酶活力的影响

用两肾一夹模型观察肾血管性高血压大鼠血压与脑，肾细胞膜ＡＴＰ酶活力的变化及前胡丙素对上述变化的影响。两肾一夹术后１６周大鼠血压由升至（２７．１±１．８）ｋＰａ，脑，肾细胞膜Ｎａ＾＋ｋ＾＋－ＡＴＰ酶活力分别下降至（７．３±２．５）和（１４．７±１．２）μｍｏｌ／（ｍｇ．ｈ），Ｃａ＾２＋ＡＴＰ活力下降至（１５．８±２．８）和（１６．１±２．３）μｍｏｌ／（ｍｇ．ｈ）。与高血压组相比，Ｐｒａ－Ｃ「２０ｍ     

国产替硝唑胶囊剂人体药物动力学及其相对生物利用度

１０名健康志愿者随机交叉一次口服１ｇ受试替耠唑胶囊或参比片剂。用高效液相－紫外（ＨＰＬＣ－ＵＶ）检测法测定其血药浓度经时过程。胶囊和片剂的主要药动力参数分别为：ｔ１／２ｋ（１４．４８±１．３８）ｈ和（１５．１０±０．８３）ｈ,Ｃｍａｘ（２０．５７±２．１３）ｍｇ．Ｌ＾－１和（１９．３６±２．３１）ｍｇ．Ｌ＾－１,ＡＵＣ（４９７．６２±５０．６２）ｍｇ．ｈ．Ｌ＾－１和（４７７．３９±４４．１５）ｍｇ     

老年慢性阻塞性肺病患者的茶碱药代动力学及临床应用

４０例慢性阻塞性肺病患者进行氨茶碱对代动力学测定结果显示，慢性喘息性支气管炎消除速率常数（Ｋ）０．０９３４±０．０１４ｈ＾－１消除半衰期（Ｔ１／２）７．６±１．２７，肺心病伴肺部感染或呼吸衰竭Ｋ０．０６５±０．０２ｈ＾－１，Ｔ１／２１１．９９±３．８７ｈ，肺心病伴心，肝功能异常者Ｋ０．０３８±０．０１６ｈ＾－１，Ｔ１／２２１．４８±９．５ｈ，比较３种给药方法的血药浓度，并探讨适全我国老年ＣＯＰＤ患     

μ—硒化卡拉胶与亚硒酸钠在兔的吸收与分布比较

给兔灌胃Ｋａｐｐａ－硒化卡拉胶３８ｍｇ．ｋｇ＾－１和亚酸钠１ｍｇ．ｋｇ＾－１，结果显示μ－ＳｅＣ组血Ｓｅ水平的ＡＵＣ０－７２ｈ为１２．８９±３．１３ｍｇ．Ｌ＾－１．ｈ，显著高于亚硒酸钠组的８．６６±１．２０ｍｇ．Ｌ＾－１．ｈ（Ｐ＜０．０５）；μ－ＳｅＣ和亚硒酸钠组的Ｃｍａｘ分别为０．５５６±０．０８６ｍｇ．Ｌ＾－１和０．４９３±０．０９１Ｓｅｍｇ．Ｌ＾－１（ｐ＞０．０５），Ｔｍａｘ分别为４．６５±     

乙酰胆碱对家兔颈动脉窦神经化学感受性单位自发电活动的影响

１５只家兔的颈动脉体窦神经标本，记录了３３个窦神经中化学感受性传入单位的电活动。实验结果：①大部分单位放电频率较低且不规则，少部分单位的放电频率高而规则；②在灌流液内加入乙酰胆碱（ＡＣｈ）２０μｇ／ｍｌ后，１６个单位的自发放电频率由１．２６±０．３１ｉｍｐ／ｓ减至０．７７±０．１９ｉｍｐ／ｓ（Ｐ＜０．０５）。③在灌流液中加入尼古丁（１、３、６、１０、和１５μｇ／ｍｌ）后，１０个单位的自发放电频率，由０．６０±０．２１ｉｍｐ／ｓ分别增加到０．９６±０．２１、１．１９±０．３０、１．２４±０．２９、１．４８±０．４１和１．８２±０．３９ｉｍｐ／ｓ，有明显的剂量依赖性（ｒ＝０．９４，Ｐ＜０．００１）。④阿托品（１０μｇ／ｍｌ）可阻断ＡＣｈ的作用，筒箭毒（１．０μｇ／ｍｌ）可阻断尼古丁作用，但对自发放电均无显著影响。上述结果提示，ＡＣｈ在颈动脉体化学感受性功能方面可能仅起调质的作用。     

双氯灭痛涂膜剂体外透皮扩散研究

本实验采用透皮扩散装置及离体小鼠、大鼠、家兔皮肤，以生理盐水为接受介质，进行了双氯灭痛涂膜剂体外透皮扩散研究。结果表明：双氯灭痛给药剂量为１．５ｍｇ／ｃｍ＾２时，其累积透皮百分率次序为：家兔（７０．９３％）〉小鼠（６２．４３％）〉大鼠（４１．４４）；稳态透皮速率（Ｊ）分别为１０７．０±６．１８，９０．２６±８．７６，６４．９２±１１．４μｇ／ｃｍ＾２·ｈ。     

乙酰胆碱对家兔颈动脉窦神经化学感受性单位自发电活动的…

１５只家兔的颈动脉体－窦神经标本，记录了３３个窦神经中化学感受性传入单位的电活动。实验结果：①大部分单位放电频率较低且不规则，少部分单位的放电频率高而规则；②在灌渡液内加入乙酰胆碱（ＡＣｈ）２０μｇ／ｍｌ后，１６个单位的自发放电频率由１．２６±０．３１ｉｍｐ／ｓ减至０．７７±０．１９ｉｍｐ／ｓ（Ｐ〈０．０５）。③在灌流液中加入尼古丁（１、３、５、１０和１５μｇ／ｍｌ）后，１０个单位的自发放电频率，     

葡萄糖氧化酶发酵动力学的研究

利用恒化器研究了葡萄糖氧化酶（ＧＯＤ）发酵动力学，建立了较为合理的恒化培养系统。对采集的数据用计算机进行回归，求得最大比生长速率μｍａｘ＝０．２２５ｈ－１、饱和常数Ｋｓ＝８．９１ｇ／Ｌ、基质得率系数ＹＧｍａｘ＝０．４９ｇ／ｇ以及维持系数ｍ＝０．０２ｇ／ｇ·ｈ。并研究了ｐＨ、ＤＯ、ＮＨ２－Ｎ、葡萄糖酸、细胞产率，酶比活及体积产酶速率等参数随比生长速率变化的规律。在Ｄ＝０．１３１ｈ－１下体积产酶速率（ＤＥ）达最大值，为３．４６×１６．６７μｍｏｌ／ｓ·Ｌ·ｈ，是分批发酵最大体积产酶速率（１．５３×１６．６７μｍｏｌ／ｓ·Ｌ·ｈ）的２．２６倍。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.