前列宁胶囊对BPH大鼠前列腺组织中IL-10 TNF-α的影响

目的：探讨前列宁胶囊（QLNC）治疗良性前列腺增生（BPH）过程中对炎症因子IL-10、TNF-α表达的影响。方法：将成年SD大鼠随机分为正常组,BPH模型组,保列治治疗组（0.5mg.kg-1.d-1）,前列宁高、中、低治疗组（2.25g.kg-1.d-1、4.5g.kg-1.d-1、9g.kg-1.d-1）,每组10只,采用去势后皮下注射丙酸睾酮法建立大鼠BPH模型（5mg.kg-1.d-1）。28天后观察各组大鼠前列腺湿重和体积、指数及病理形态学变化,RT-PCR法检测大鼠前列腺组织中IL-10、TNF-α表达情况。结果：QLNC组大鼠前列腺体积、指数较模型组明显减小,病理变化较模型组明显改善;RT-PCR显示QLNC能增强大鼠前列腺组织中IL-10表达,降低TNF-α表达。结论：QLNC能明显抑制大鼠前列腺组织增生,降低炎症因子TNF-α在前列腺组织中的表达,提高IL-10在前列腺组织中的表达,表明QLNC通过抑制炎症因子表达可能是治疗BPH的机制之一。     

Inhibitory Effect of Thymoquinone on Testosterone‐Induced Benign Prostatic Hyperplasia in Wistar Rats

This study addresses the possible protective effects of thymoquinone (TQ) against the development of experimentally‐induced benign prostatic hyperplasia (BPH) in Wistar rats. Eighteen adult male rats were divided into three groups; the negative control group (n = 6) received vehicle, and two groups received subcutaneous testosterone injection (3 mg/kg). Animals receiving testosterone were randomized to untreated BPH group (n = 6) and BPH + TQ treated group (n = 6, 50 mg/kg orally for 14 days). Histological changes and the mRNA levels of transforming growth factor‐β₁ (TGF‐β₁) and vascular endothelial growth factor‐A (VEGF‐A) were analyzed. Additionally, dihydrotestosterone and interleukin‐6 (IL‐6) serum levels were determined. The presented research shows significant increases in prostate weight/body weight ratio, prostate epithelial thickness, serum IL‐6 and dihydrotestosterone levels, and the prostatic expressions of TGF‐β₁ and VEGF‐A in the untreated BPH rats. Histological examination of the prostate tissues in the BPH rats showed an elevated level of proliferation in the stromal area and glandular epithelia with abundant intraluminal papillary folds. However, a reduction in prostate weight/body weight ratio, epithelial hyperplasia, serum IL‐6 levels, and the expressions of TGF‐β₁ and VEGF‐A were observed in the BPH + TQ treated rats compared with the untreated BPH rats. The findings support TQ as a useful natural treatment for animal BPH model. Copyright © 2017 John Wiley & Sons, Ltd.     

补骨脂素对良性增生前列腺细胞增殖的影响

目的:探讨补骨脂素抑制良性前列腺增生的作用机制.方法:补骨脂素作用雄性去势大鼠注射丙酸睾酮后的前列腺湿重、前列腺指数、PCNA指数及前列腺组织病理学改变.结果:实验组大鼠前列腺湿重、前列腺指数和PCNA指数均显著低于对照组.结论:补骨脂素能显著抑制模型大鼠的良性前列腺增生,其机制可能是通过降低前列腺细胞增殖而实现的.     

针刺对实验性大鼠前列腺组织中Ki-67和bFGF表达水平的影响

目的观察针刺对前列腺增生症(BPH)的影响,探讨其作用机制。方法将雄性SD大鼠随机分为正常组、模型组、药物组、针刺组和针药结合组,采用去势后皮下注射丙酸睾丸酮4 mg/kg的方法来复制大鼠前列腺增生的模型,同时给予相应治疗,共进行30 d。造模和治疗结束后,测量各组大鼠前列腺湿质量指数、体积,光镜和电镜下观察前列腺组织的形态学变化,用免疫组化法检测Ki-67和bFGF在各组大鼠前列腺组织中表达的差异。结果模型组大鼠前列腺湿质量指数、体积及组织中的Ki-67和bFGF的表达水平与假手术组比较明显升高,腺上皮细胞数目与假手术组比较明显增加(P均<0.05);药物组、针刺组及针药结合组大鼠的前列腺湿质量指数、体积及组织中的Ki-67和bFGF的表达水平与模型组比较明显降低(P均<0.05),腺上皮细胞数目与模型组比较明显减少(P<0.05)。3个治疗组大鼠的前列腺湿质量指数、体积和组织中的Ki-67和bFGF的表达水平比较无显著性差异(P均>0.05)。结论针刺能够明显降低前列腺增生大鼠的前列腺湿质量指数和体积,抑制Ki-67和bFGF在前列腺组织中的表达,从而达到抑制前列腺增生的作用。     

中药锁阳水煎剂对大鼠前列腺增生及氧化应激的影响

目的：研究锁阳水煎液对大鼠前列腺增生的影响及作用机制。方法：60只SD大鼠随机均分为空白对照组、前列腺增生模型组、前列康治疗组、锁阳水煎液高、中、低剂量治疗组6组。除空白对照组外,各组大鼠注射丙酸睾酮制作前列腺增生模型,模型组和空白对照组以生理盐水灌胃,前列康治疗组用前列康（570mg·（kg·d）-1）灌胃、锁阳干预组用锁阳水煎液120、80、40mg·（kg·d）-1灌胃。60天后处死大鼠,观察前列腺湿重及指数的变化,采用试剂盒法检测SOD、GSP活性和MDA含量。结果：各治疗组大鼠前列腺湿重和指数均显著低于模型组;SOD和GSH明显高于模型组,且MDA含量明显低于模型组。结论：锁阳水煎液可抑制大鼠前列腺增生,其作用可能与改善组织中氧化应激水平有关。     

桂枝茯苓胶囊对实验性大鼠前列腺增生的影响

目的观察桂枝茯苓胶囊对丙酸睾丸素致大鼠前列腺增生症的影响。方法大鼠去睾丸7d后,sc丙酸睾丸素(3mg/kg)建立大鼠前列腺增生症病理模型,同时连续ig桂枝茯苓胶囊(2.16、1.08、0.54g/kg)30d,末次给药后1h,处死动物,取前列腺称质量,测定体积并进行病理学检查。结果桂枝茯苓胶囊给药组大鼠前列腺质量和体积均低于模型组(P<0.05,0.01);病理镜检显示桂枝茯苓胶囊给药组前列腺腺腔组织扩张较轻,且增生腺上皮高度较模型组低,尤以大、中剂量组最为明显(P<0.05,0.01)。结论桂枝茯苓胶囊可明显抑制大鼠前列腺组织增生。     

Inhibitory effects of crude polysaccharides from semen vaccariae on benign prostatic hyperplasia in mice

Ethnopharmacological relevance

Semen vaccariae, the seeds of Vaccaria Segatalis, is a famous traditional herb for the treatment of stranguria disease, e.g. benign prostatic hyperplasia (BPH). The present study investigated the effect of crude polysaccharide from Semen vaccariae (SVCP) on BPH in mice in order to further understand the efficacy substance of this medicinal plant.

Materials and methods

Sixty healthy adult male Kunming mice were randomly divided into five groups with 12 mice for each group: sham-operated group, BPH model group; BPH model group treated with Pule'an at a dose of 1.20 g/kg; BPH model group treated with SVCP at dose levels of 1.67 g/kg and 0.42 g/kg. The drugs were administered orally once a day consecutively for 12 days. The BPH in mice was created by subcutaneous injection of testosterone propionate for 5 mg/(kg d) to the uncastrated mice once a day consecutively for 12 days. The inhibitory effects on BPH of SVCP were evaluated by prostatic index, testicular index and histopathologic examination.

Results

Comparing with BPH model group, BPH mice fed with SVCP exhibited significant differences in both the prostatic index and testicular index. On the aspect of histopathology, the SVCP treated BPH mice exhibited similar histological aspects observed in the mice of sham group while the BPH mice exhibited typical features of prostate glandular hyperplasia.

Conclusions

SVCP exhibited a significant inhibitory activity on BPH by reducing the prostatic index, testicular index, and ameliorating the pathomorphology. The results indicated that the polysaccharide should be the main efficacy substance of Semen vaccariae and may contribute, in a large part, to Semen vaccariae's traditional medicinal use for the treatment of stranguria disease.     

雌二醇对丙酸睾酮诱导去势大鼠前列腺增生的作用

目的探讨雌二醇对丙酸睾酮诱导去势大鼠前列腺增生的作用。方法建立丙酸睾酮诱导去势大鼠前列腺增生模型,连续皮下注射苯甲酸雌二醇(2.5 mg/kg)4周后处死动物,分离前列腺,测量前列腺体积和湿质量,计算前列腺指数。结果与丙酸睾酮组相比,雌二醇组大鼠前列腺体积、前列腺湿质量和前列腺指数无明显改变(P均>0.05)。结论雌二醇不能抑制丙酸睾酮诱导去势大鼠前列腺增生。     

油菜花粉活性部位对尿生殖窦诱导的鼠前列腺增生的治疗作用

目的：研究油菜花粉总脂肪酸部位（PN1）和总黄酮部位（PN2）对前列腺增生的治疗作用。方法：采用前列腺腹叶植入16-18日胎龄大鼠尿生殖窦组织致大鼠前列腺增生模型,通过灌胃给予PN1、PN2、PN1+PN2进行治疗,连续给药33天后,取前列腺,分离出背叶及腹叶,称重、测定体积,计算前列腺湿重及指数,测定前列腺液中卵磷脂小体密度及白细胞数量,并检测血清酸性磷酸酶（ACP）及前列腺特异性酸性磷酸酶（PACP）的活性。结果：与模型组相比,PN1高剂量组,PN1+PN2高、中剂量组前列腺重量明显减少（P<0.01,P<0.05）,其中PN1+PN2的高剂量组前列腺指数明显降低（P<0.01）;PN1高剂量组、PN2高剂量组前列腺液中卵磷脂小体密度明显增加（P<0.05）,PN1各剂量,PN2中、低剂量组和PN1+PN2高剂量组均白细胞数目明显降低（P<0.01,P<0.05）;PN2高剂量组PACP活性明显降低（P<0.05）,PN1+PN2中剂量组ACP活性明显降低（P<0.05）。结论：本工艺制得的油菜花粉总脂肪酸部位及总脂肪部位与总黄酮部位联合使用,有明显抗前列腺增生的作用,有望开发成相关的药物。     

克癃胶囊对前列腺增生大鼠模型及Nrf2/ARE信号通路的影响

目的:探讨克癃胶囊对丙酸睾酮诱导的大鼠前列腺增生模型的影响及抗氧化作用机制。方法:将40只雄性SD大鼠随机分为正常组,模型组,克癃胶囊低、高剂量组(3.6,7.2 g·kg-1),核因子E2相关因子2(Nrf2)干预剂组,每组8只。除正常组外,其余各组均皮下注射丙酸睾酮建立前列腺增生的大鼠模型,同时给予药物灌服,连续4周。观察不同药物对前列腺湿重、前列腺指数的影响,苏木素-伊红(HE)观察病理组织形态变化,酶联免疫吸附测定法(ELISA)检测血清样品中丙二醛(MDA)和谷胱甘肽(GSH)的含量,实时荧光定量聚合酶链式反应(Real-time PCR)检测前列腺组织中Nrf2/ARE信号通路抗氧化因子Nrf2,血细素单加氧酶-1(HO-1),醌氧化还原酶1(NQO1)mRNA的表达水平。结果:与正常组比较,模型组大鼠的前列腺湿重及前列腺指数升高,前列腺上皮皱褶增生,大鼠血清GSH含量明显降低,Nrf2,HO-1和NQO1 mRNA表达明显降低(P0.05,P0.01)。与模型组比较,克癃胶囊明显降低前列腺指数,改善前列腺上皮组织的增生,促进大鼠血清GSH的含量增加(P0.01);克癃胶囊能上调Nrf2,HO-1和NQO1的mRNA表达水平(P0.05,P0.01)。结论:克癃胶囊能明显抑制大鼠的前列腺增生,低剂量的克癃胶囊可激活Nrf2/ARE信号通路,提高机体抗氧化能力。     

补肾活血方对BPH大鼠前列腺组织中VEGF、bFGF表达的影响

目的：评价补肾活血方对BPH大鼠的疗效,并探讨其作用机理。方法：实验研究采用SD大鼠去势后皮下注射丙酸睾丸酮法复制BPH模型,用形态计量学方法研究各组前列腺组织的形态改变;用免疫组化法研究实验各组前列腺组织的血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）的表达。结果：补肾活血方可以缩小增生前列腺腺体的体积,并抑制VEGF、bFGF的表达。结论：补肾活血方用于治疗BPH,能缩小前列腺体积,作用机理是可能是通过抑制VEGF、bFGF的表达,进而抑制前列腺增生。     

Protective Effects of Lepidium meyenii (Maca) Aqueous Extract and Lycopene on Testosterone Propionate‐Induced Prostatic Hyperplasia in Mice

The inhibitory effect of maca extractant, lycopene, and their combination was evaluated in benign prostatic hyperplasia (BPH) mice induced by testosterone propionate. Mice were divided into a saline group, solvent control group and testosterone propionate‐induced BPH mice [BPH model group, solvent BPH model group, benzyl glucosinolate group (1.44 mg/kg), maca group (60 mg/kg), lycopene treated (15, 5, and 2.5 mg/kg), maca (30 mg/kg) combine lycopene treated (7.5, 2.5, and 1.25 mg/kg), and finasteride treated]. Benzyl glucosinolate was used in order to evaluate its pharmacological activity on BPH to find out whether it is the major active component of maca aqueous extract. Finasteride was used as positive control. The compounds were administered once for 30 successive days. Compared with solvent BPH model group, BPH mice fed with maca (30 mg/kg) and lycopene (7.5 mg/kg) combination exhibited significant reductions in the prostatic index, prostatic acid phospatase, estradiol, testosterone, and dihydrotestosterone levels in serum. They also had similar histological compared with those aspects observed in the mice in the solvent control group. The results indicated that combination of maca and lycopene synergistically inhibits BPH in mice. Copyright © 2017 John Wiley & Sons, Ltd.     

前列安通片对BPH大鼠前列腺组织中 VEGF，bFGF表达的影响

目的：观察前列安通片对前列腺增生(BPH)大鼠增生前列腺组织的影响，并探讨其作用机制。方法：采用SD大鼠去势后皮下注射丙酸睾丸酮法复制BPH模型，用形态计量学方法研究各组前列腺组织的形态改变；用免疫组化法研究实验各组前列腺组织的血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)的表达。结果：前列安通片高、中、低各剂量组(6.24，3.12，1.56 g·kg-1)及阳性药保列治组(1.67 g·kg-1)与模型组比较，前列腺质量、前列腺体积、前列腺指数均明显降低(P<0.01)；腺体平均面积、平均周长降低，相同面积内腺体总数目增加(P<0.01)；前列安通片各剂量组bFGF表达显著低于模型组(P<0.01)，高剂量组前列腺组织中VEGF表达明显低于模型组(P<0.05)。结论：前列安通片能缩小前列腺体积，对BPH有一定的治疗作用，作用机制与抑制VEGF，bFGF的表达有关。     

金匮肾气丸化裁方对良性前列腺增生大鼠前列腺组织)VEGF、IGF-1 表达的影响

目的 研究金匮肾气丸化裁方对大鼠前列腺增生模型前列腺组织VEGF和IGF-1表达的影响.方法 去势加丙酸睾酮注射法建立大鼠前列腺增生模型,免疫组化学法检测前列腺组织中血管内皮生长因子（VEGF）的表达,RT-PCR法检测类胰岛素生长因子-1（IGF-1）mRNA的水平.结果 金匮肾气丸化裁方中剂量组明显降低前列腺增生组织中VEGF和IGF-1 mRNA的水平.结论金匮肾气丸化裁抑制大鼠前列腺增生可能与调控生长因子表达水平有关.     

癃必舒胶囊对大鼠实验性前列腺增生的影响

目的观察癃必舒胶囊对丙酸睾丸素致大鼠实验性前列腺增生的影响。方法大鼠去睾丸7d后,皮下注射(sc)丙酸睾丸素(3 mg/kg)制备大鼠前列腺增生症病理模型。同时连续灌胃(ig)癃必舒胶囊(0.25,0.5,1.0 g.kg-1)21 d,末次给药后1 h处死大鼠,取前列腺称重,计算前列腺系数,并进行病理检查。结果癃必舒胶囊组前列腺湿干系数明显小于丙酸睾丸素组。病理学检查显示癃必舒胶囊组大鼠前列腺处于增生状态的腺体数明显低于丙酸睾丸素组,非增生状态的腺体数多于丙酸睾丸素组。结论癃必舒胶囊对大鼠实验性前列腺组织增生有明显抑制作用。     

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??OBJECTIVE To investigate the inhibitory effect and possible mechanisms of puerariae isoflavone(PI) on prostatic hyperplasia induced by testosterone propionate.METHODS Forty-eight male Wistar rats were randomly divided into six groups according to their body weight including normal control group, model group, 40, 80, 160 mg??kg^-1??d^-1 PI group, and finasteride positive control group. In addition to the sham operation for rats in the normal control group, the rats in other five groups performed castration surgery. After the restoration, the five groups of rats were subcutaneously injected with testosterone propionate (10 mg??kg^-1??d^-1) for 10 d to establish a benign prostatic hyperplasia model and then the subcutaneous injection was maintained every 2 d. High, middle and low dose PI groups were intragastrically administered (40, 80, 160 mg??kg^-1??d^-1) from the second day when the benign prostatic hyperplasia model was successfully constructed. The positive control group was given finasteride (1.0 mg??kg^-1??d^-1) .Rats in normal and model groups were given an equal volume of saline for 28 d. After the last administration, the prostate and seminal vesicles were separated under anesthesia in rats, the wet weight and volume of the prostate and seminal vesicles were measured. The prostate and seminal vesicles index were calculated too. Rat blood was drawn and dihydrotestosterone(DHT) and estradiol (E2) in the serum were measured. Nitric oxide (NO), nitric oxide synthase (NOS), superoxide dismutase (SOD) and malondialdehyde (MDA) levels in prostate tissues were measured. The prostate tissue in each group was randomly selected for HE staining. The pathological structure of the prostate tissue was observed under an optical microscope.RESULTS Compared with the normal control group, the prostate gland index and seminal vesicle gland index of the model group increased significantly (P<0.01), and the DHT and E2 levels in serum increased significantly (P<0.01). MDA content was increased while NO levels, NOS and SOD activities were significantly decreased (P<0.01). HE staining showed that the size of the prostate gland in the model group was different, there were obvious dilation, hyperplasia and papillary protrusions, and the cavity was full of pink and homogeneous density. The interstitial tissue showed obvious dilations of blood vessels, infiltration of inflammatory cells, and proliferation of fibrous connective tissues. Compared with the model group, the index and volume of prostate and seminal vesicles in the PI and positive control groups were significantly decreased (P<0.05 or P<0.01), and the levels of serum DHT and E2 in the middle and high doses PI groups were significantly lower (P<0.05 or P<0.01). In all treatment groups, MDA content was decreased and NO, NOS, and SOD levels were increased (P<0.05 or P<0.01) except the low-dose PI groups. There was moderately hyperplasia in low-dose PI group, mild prostatic hyperplasia in positive control group and middle-dose PI group, basically no hyperplasia in high-dose PI group.CONCLUSION PI has a certain inhibitory effect on prostate hyperplasia induced by testosterone propionate, especially in the medium and high dose PI groups. The mechanism may be related to the effects of pueraria isoflavone on antioxidant,free radical scavenging in vivo, increasing NOS activity and increasing NO level.     

A study of prostane in the treatment of benign prostatic hyperplasia

A clinical trial was conducted in a study group of 70 males diagnosed with symptomatic benign prostatic hypertrophy (BPH) (synonym of benign prostatic hyperplasia). They were administered Prostane, a herbal formulation, at a dose of two tablets a day for 1 year and monitored every 4 months during the study period. Analysis of the results showed an improvement in the symptom score of the American Urological Association symptom index rating. There was total relief in pain and haematuria in all the patients (100%); dribbling of urine decreased in 67%, dysuria in 50%, urgency in 60% and hesitancy in 40%. Blood urea levels were within the normal range in 70% of the patients and in the range 31-40 mg/dL in the remaining patients of the study group. Serum prostate specific antigen levels returned to normal in 56% of patients and were in the range 4.1-5.0 ng/mL in 25% of patients. There was a decrease in prostate specific antigen values which were >6 ng/mL in 9 patients at the commencement of the trial. Uroflowmetry studies showed that the peak flow increased from 12.6 to 30.7 s (p<0.001) and the void volume from 60.72 to 660 mL (p<0.001), the latent period reduced from 12.78 s to 2.61 s; the flow time from 57.01 s to 20.17 s and the residual volume from 620 mL to 20 mL (p<0.001). From these results, it is evident that Prostane was effective in alleviating symptoms, reducing prostate specific antigen values and normalizing uroflow dynamics in patients with benign prostatic hypertrophy.     

当归贝母苦参丸对良性前列腺增生模型小鼠PCNA、bcl-2和bax表达的影响

目的 探讨当归贝母苦参丸对良性前列腺增生(BPH)模型小鼠的影响及作用机理.方法 将60只雄性小鼠,按照随机数字表法随机分为6组:空白组,模型组,阳性对照组,高、中、低剂量治疗组.空白组给予腹腔注射12.5 mg/kg·d-1橄榄油,其他各组采用腹腔注射内酸睾酮原液制作小鼠BPH模型,同时高、中、低剂量治疗组分别予高、...     

中成药小金丸治疗良性前列腺增生的疗效观察

目的:观察中成药小金丸治疗良性前列腺增生的有效性和安全性。方法:将160例良性前列腺增生病人随机分成两组,每组80例。小金丸组,予小金丸0.6g×2,po,bid;非那雄胺组,予非那雄胺5mg,po,qd,疗程均为6mo。结果:用药后,小金丸组国际前列腺症状评分(IPSS)下降了(6.9±0.7)分,非那雄胺组下降了(6.4±0.7)分,两组间比较无显著差异(p>0.05)。用药后两组最大尿流率(Qmax)和残余尿改善值差异无显著意义(p>0.05),前列腺体积和血清前列腺特异性抗原(PSA)下降幅度差异无显著意义(p>0.05)。结论:小金丸治疗良性前列腺增生症安全、有效。     

Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat

Ethanol extract of Cissus quadrangularis was evaluated for its anti-osteoporotic activity in ovariectomized rat model of osteoporosis at two different dose levels of 500 and 750 mg/kg per day. Healthy female albino rats were divided into five groups of six animals each. First group was sham operated and served as control. All the remaining groups were ovariectomized. Group 2 was fed with equivolume of saline and served as ovariectomized control. Groups 3-5 were orally treated with Raloxifen (5.4 mg/kg) and ethanol extract of Cissus quadrangularis (500 and 750 mg/kg), respectively. The findings assessed on the basis of biomechanical, biochemical and histopathological parameters showed that the ethanol extract of the plant had a definite antiosteoporotic effect.