Rapid communication: acute exposure to mercury from dental amalgam does not affect the levels of C-reactive protein or interleukin-6 in peripheral blood

In a previous study, a significant increase in serum interleukin-6 (IL-6) was apparent after an acute low-level mercury (Hg) exposure, achieved by removal of amalgam fillings (Loftenius et al., 1998). In the present study, 11 healthy volunteers were exposed to an oral dose of 1 g of pulverized amalgam powder. Hg, IL-6, and C-reactive protein (CRP) levels in plasma were followed before and up to 72 h after exposure. The Hg levels were low and stable prior to exposure and increased rapidly after exposure. The median Hg increase was 12.9 nmol/L, which is considerably higher than in the previous study. No significant change over time was observed for IL-6 and CRP levels. Therefore, it cannot be ruled out that our previous finding of increasing IL-6 levels detected after acute low-level Hg exposure through removal of amalgam fillings was due to the dental treatment per se.     

Minor changes in serum levels of cytokines after removal of amalgam restorations

Dental amalgam restorations release mercury and silver which is absorbed and distributed in the body. Animal studies have shown that both elements may interfere with the host by activation of the immune system in genetically susceptible strains at exposure levels relevant to those from dental amalgam restorations. The aim of this study was to test the hypothesis of no change over time in concentrations of a number of immune mediators in serum after removal of all dental amalgam restorations in patients with health complaints attributed to their amalgam restorations and compare with a healthy reference group. Twenty patients previously examined at a specialty unit for health complaints attributed to dental materials were included in a clinical trial and had all amalgam restorations replaced with other dental restorative materials. Serum samples were collected before amalgam removal and 3 and 12 months after the removal was finished. Twenty blood donors matched for age and gender were used as comparison group. A fluorescent bead-based (Luminex) immunoassay kit was used to measure cytokines, chemokines and growth factors in serum. At baseline, the patient group had slightly higher values for GM-CSF, IL-6, IL-2R, IFN-alpha, IL-7, and IL-12p40/p70 compared with the reference group. After amalgam removal a decrease towards the median value of the reference group was found for GM-CSF, IL-8, and IL-7. In conclusion, removal of all dental amalgam restorations and replacement with other dental restorative materials was associated with decreased concentrations of Th1-type proinflammatory markers in serum.     

犀角地黄汤合方含药血清调节ITP患者Teff/Treg分化及功能的研究#br#

目的　探究犀角地黄汤合方含药血清对免疫性血小板减少症（ITP）患者效应性T细胞（Teff）/调节性T细胞（Treg）分化的影响,并探讨磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（Akt）/哺乳动物雷帕霉素靶蛋白（mTOR）信号通路在其中的作用机制。方法　采用随机数字表法将20只大鼠分为含药血清组和空白血清组,每组10只,分别使用犀角地黄汤合方颗粒和蒸馏水连续灌胃3 d,收取2组大鼠全血制备含药血清和空白血清。收集健康志愿者和ITP患者外周血,提取外周血单个核细胞（PBMC）,磁珠分选CD4+ T细胞,抗CD3/CD28抗体培养体系活化后,分为对照组、模型组和实验组。对照组为健康志愿者CD4+ T细胞,模型组及实验组为ITP患者CD4+ T细胞,实验组采用含药血清干预72 h,对照组及模型组采用大鼠空白血清干预72 h。流式细胞术检测各组CD4+ T细胞中Teff/Treg比例;酶联免疫吸附试验（ELISA）检测各组培养上清液中白细胞介素（IL）-2、干扰素（IFN）-γ、肿瘤坏死因子（TNF）-α、IL-6、IL-17及Treg细胞因子转化生长因子（TGF）-β、IL-10水平;Western blot检测PI3K、Akt、mTOR蛋白及磷酸化蛋白水平。结果　CD4阳性率为（99.44±0.01）%。与对照组相比,模型组CD4+ T细胞中Teff细胞比例明显升高,Treg比例明显降低,Teff/Treg比值增大,IL-2、IL-6、IL-17、IFN-γ、TNF-α水平以及PI3K、p-PI3K、mTOR、p-mTOR蛋白水平升高,TGF-β、IL-10、p-Akt蛋白水平降低（P＜0.05）;与模型组相比,实验组CD4+ T细胞中Teff细胞比例降低,Treg细胞比例升高,Teff/Treg比值减小,IL-2、IL-6、IL-17、IFN-γ、TNF-α水平以及PI3K、p-PI3K、mTOR、p-mTOR蛋白水平降低,TGF-β、IL-10水平升高（P＜0.05）。结论　犀角地黄汤合方含药血清能够调节ITP患者Teff/Treg的分化及细胞因子的分泌,可能与抑制PI3K/Akt/mTOR信号通路PI3K、mTOR蛋白表达及其磷酸化水平有关。     

地佐辛静脉自控镇痛对肺叶切除术后免疫功能的影响

目的:观察地佐辛静脉自控镇痛对肺叶切除术后免疫功能的影响.方法:选取60例拟行开胸肺叶切除术的患者随机分为对照组和DEZ组,每组30例.DEZ组术后PCA泵注0.5 mg·ml^-1地佐辛,对照组术后PCA泵注0.9%氯化钠.采集患者麻醉诱导前(T0)、术后4 h(T1)、术后24 h(T2)和术后48 h(T3)血标本,测定血清CRP、TNF-α、IL-2、IL-6和IL-10水平,以及外周血淋巴细胞(L)计数及淋巴细胞中CD4⁺T淋巴细胞、CD8⁺T淋巴细胞、自然杀伤细胞(NK)比例的变化.于T1、T2和T3时点随访患者,记录患者视觉模拟评分(VAS)和数字镇静评分(NSS).结果:肺叶切除术后患者血清CRP、TNF-α、IL-6和IL-10的水平较术前明显升高(P <0.05),IL-2较术前降低(P <0.05);术后外周血淋巴细胞计数及CD4⁺T淋巴细胞比例和NK细胞比例较术前降低(P <0.05);地佐辛静脉自控镇痛能明显降低术后血清CRP、IL-6,升高IL-2及CD4⁺T淋巴细胞比例(P <0.05);地佐辛组患者术后24 h的VAS评分低于对照组(P <0.05).结论:地佐辛静脉镇痛具有免疫调节作用,可改善肺叶切除术后的免疫抑制,并减轻炎症反应.     

羟考酮联合氟比洛芬酯对于乳腺癌术后患者的镇痛效果及应激、免疫水平的影响

目的：探讨羟考酮联合氟比洛芬酯用于乳腺癌术后患者的镇痛效果及应激、免疫水平的变化。方法：选择某院2016年5月-2018年5月全麻手术下行乳腺癌根治术的患者100例进行前瞻性研究。患者术前按照随机数字表分配随机方案，设置A组（50例）为羟考酮联合氟比洛芬酯，B组（50例）为芬太尼联合氟比洛芬酯。所有患者均采用镇痛泵自行控制术后疼痛（PCIA）。所有患者均记录手术时间、麻醉时间及出血量等一般情况。记录患者术后12，24，48 h的疼痛VAS评分。采集同期的患者外周静脉血，测定患者T淋巴细胞亚群（CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺）及NK细胞水平。用酶联免疫吸附法测定IL-2，IL-8，IL-10浓度。记录患者按压次数及镇痛药物追加使用例数，不良反应发生情况。结果：2组患者手术时间、麻醉时间及出血量等一般情况无差异。与B组患者相比，A组患者的VAS评分在12，24，48 h均出现了显著降低（P<0.05）。与术前相比，2组术后12 h及术后24 h的CD3⁺、CD4⁺、CD4⁺/CD8⁺及NK细胞明显降低（P<0.05）；A组术后12 h及术后24 h的CD3⁺、CD4⁺、CD4⁺/CD8⁺及NK细胞均高于B组，术后12 h的CD8⁺低于B组（P<0.05）；术后48 h的CD3⁺、CD4⁺、CD4⁺/CD8⁺、CD8⁺及NK细胞2组差异均无显著性（P>0.05）。2组术后12 h及术后24 h相比术前的IL-2及IL-8浓度降低明显（P<0.05）；IL-10浓度升高明显（P<0.05）。A组术后12 h及24 h的IL-2及IL-8浓度均高于B组，IL-10浓度均低于B组（P<0.05）；术后48 h的IL-2、IL-8及IL-10浓度2组差异均无显著性差异（P>0.05）。A组与B组相比，术后48 h内总的按压镇痛泵的次数更少，且总的泵消耗量也较小（P<0.05）。而A组患者术后不良反应发生例数术仅4例，B组患者不良反应发生例数11例，2组间有显著性差异（P<0.05）。结论：羟考酮联合氟比洛芬酯应用于乳腺癌术后患者的自控镇痛安全有效，降低了术后疼痛感，还可以减少术后镇痛药用量，降低了术后不良反应的发生且调节了T淋巴细胞亚群和NK细胞水平和IL-2，IL-8及IL-10浓度，值得临床麻醉推广。     

硼替佐米对佐剂性关节炎模型大鼠的免疫调节作用及其机制研究

目的:研究硼替佐米对佐剂性关节炎模型大鼠的免疫调节作用及其机制。方法:取大鼠随机分成正常对照组、模型组、阳性对照组(甲氨蝶呤0·5mg·kg-1,3d1次)和硼替佐米低、高剂量组(0·2、1·0mg·kg-1,每日1次),每组10只,后4组用完全弗氏佐剂建立佐剂性关节炎模型,建模给药后第16天开始尾静脉注射相应药物,给药15d。评价建模给药后各组小鼠关节炎指数(3d1次),检测建模给药30d后各组大鼠外周血CD3+T、CD4+T、CD8+T细胞含量和CD4+T/CD8+T细胞比例,以及血清中γ干扰素(IFN-γ)、白细胞介素(IL)-4、IL-17水平。结果:与正常对照组比较,模型组大鼠关节炎指数评分更高,外周血CD4+T、CD4+T/CD8+T细胞比例及血清中IFN-γ、IL-4表达均明显增强(P<0·05)。与模型组比较,硼替佐米高剂量组和阳性对照组大鼠关节炎指数评分更低,外周血CD4+T、CD4+T/CD8+T细胞比例及血清中IFN-γ、IL-17表达均明显降低(P<0·05),仅硼替佐米高剂量组IL-4表达明显增加(P<0·05);硼替佐米低剂量组上述指标均无明显差异。结论:硼替佐米可能通过调节T细胞功能及其释放的细胞因子水平减缓佐剂性关节炎模型大鼠的关节肿胀程度。     

CD4^＋CD25^＋调节性T淋巴细胞在急性脑梗死患者外周血中表达的初步研究

目的初步探讨CD4^＋CD25^＋调节性T淋巴细胞在急性脑梗死患者外周血中的表达情况。方法采用流式细胞术分别检测取98例急性脑梗死患者和50名健康体检者外周血中CD4^＋CD25^＋T细胞所占比例,并用双抗体夹心法检测各组血清TGF-B、IL-10和IL-2。结果急性脑梗死患者外周血中CD4^＋CD25^＋T细胞水平显著高于对照组（P〈O．01）,血清中TGF-β、IL-10和IL-2显著高于对照组,差异有统计学意义（P〈0．01）。结论CD4^＋CD25^＋T细胞在急性脑梗死患者外周血中表达显著升高,血清中的相关细胞因子参与诱导CD4^＋CD25^＋T细胞的生成,提示CD4^＋CD25^＋T细胞可能参与了急性脑梗死的发生、发展。     

胆结石患者治疗前后血清白介素及T淋巴细胞亚群水平的变化

目的观察胆结石患者治疗前后多项血清白介素及T淋巴细胞亚群水平的变化。方法选择72例胆结石患者作为观察组,另选同期健康体检的健康人30例作为对照组,观察组于术前和术后5d检测血清白介素水平和T淋巴细胞亚群水平。结果观察组术前血清IL-6、IL-8和TNF-α水平分别为（61．5±4．8）ng／L、（246．2±15．3）n#L和（1．79±0．72）mg／L,均显著高于术后和健康对照组（均P〈0．05）,而术后血清IL-6、IL-8和TNF-α水平与健康对照组比较均差异无统计学意义（均P〉0．05）;观察组术前CD3＋、CD4＋和CD8＋T细胞的比例分别为（0．53±0．06）％、（0．28±0．05）％和（0．21±0．04）％,均显著低于术后和健康对照组（均P〈0．05）,而术后CD3＋、CD4＋和CD8＋T细胞的比例和健康对照组比较均差异元统计学意义（均P〉0．05）。结论胆结石患者存在明显的血清IL-6、IL-8和TNF-α以及CD3＋、CD4＋和CD8＋T细胞水平异常,手术去除胆结石不但能消除炎症,而且有助于患者免疫功能的恢复。     

急性白血病患者CD4+CD25+FoxP3+调节性T细胞、IL-17和IL-23的表达及临床意义

目的观察急性白血病(AL)患者外周血中CD4+CD25+FoxP3+调节性T细胞比例、血清白细胞介素(IL)17和IL-23的表达及其临床意义。方法用流式细胞术测定25例AL患者[急性髓系白血病(AML)15例,急性淋巴细胞白血病(ALL)10例]和10例健康体检者(对照组)外周血中CD4+CD25+FoxP3+调节性T细胞比例,ELISA法测定血清中IL-17和IL-23的表达,分析其间的相关性。结果 AML患者组CD4+CD25+FoxP3+调节性T细胞比例显著高于对照组[(6.52±3.25)vs.(3.58±1.02)](P<0.05);AML和ALL组血清IL-17水平显著高于对照组[(7.21±2.00),(7.47±1.63)vs.(4.52±1.62)](P<0.05)。AL患者血清IL-17和IL-23水平显著正相关(P<0.05)。结论 CD4+CD25+Foxp3+调节性T细胞和IL-17细胞在AML的免疫功能抑制中可能有重要作用,而IL-17细胞在ALL的免疫功能抑制中可能产生作用。     

Combination of carboxyamidotriazole and 1-Methyl-L-tryptophan has synergistic inhibtory effects on programmed death 1 expression

OBJECTIVE To evaluate whether the IDO1 inhibitor 1-methyl-L-tryptophan(1-MT)combine calcium influx inhibitor carboxyamidotriazole(CAI)could further enhance the suppression of programmed death 1(PD-1)in CD8~+T cells and investigate the curative effect of the combined use.METHODS CD8~+T cells were isolated from normal mice spleen by negative selection using magnetic cell separation.The isolated CD8~+T cells were cultured in RPMI 1640 medium containing 10%FBS and 100 U·mL~(-1)IL-2 and activated by the addition of anti-CD3 and anti-CD28(1 g·L~(-1) each mabs).CD8~+T cells were pretreated for 48 h with drug and the fluo-3 as a marker of intracellular calcium concentration was detected by flow cytometry.The calcineurin(Ca N)levels were assayed with ELISA in CD8~+T cells after 48 h incubation with 10μm CAI.The nuclear translocations of NFAT and AHR were detected by immunofluorescent staining after 48 h of drug treatment.The expression of PD-1 in CD8~+T cells was analyzed by flow cytometry.RESULTS Intracellular fluorescent intensity was markedly debase due to CAI treatment(P<0.01).Meanwhile,the changes of CaN content had a resembled correlation(P<0.01).Immunofluorescence experiment showed that after combination therapy the transfer of NFAT and AHR in nuclear substantially reduced.Flow cytometry revealed that after the combination caused a significant decrease in PD-1 expression in CD8~+T cells.CONCLUSION CAI and 1-MT could inhibit markedly the expression of PD-1 in CD8~+T cells by inhibiting the nuclear translocation of NFAT and AHR,respectively and the combination of them has synergetic effect.     

Effect of coronary percutaneous revascularization on interferon-gamma and interleukin-10 producing CD4+ T cells during acute myocardial infarction

T lymphocytes play an important role in the induction and progression of acute coronary syndromes (ACS). To gain insight into how different T cell subsets can influence ACS, we analyzed the frequencies of circulating CD4+ T cells producing either pro-inflammatory interferon(IFN)-gamma or anti-inflammatory interleukin (IL)-10 in subjects presenting with ST-elevation myocardial infarction (STEMI). The effect of coronary bare metal (BS) and paclitaxel-eluting stent (PES) on the balance between CD4+IFN-gamma+ and CD4+IL-10+ lymphocytes was also investigated. Peripheral blood mononuclear cells (PBMC) were isolated from 38 consecutive patients with STEMI before and 48 hrs or 6 days after implantation of either BS or PES. Twenty patients with no history of coronary artery disease were included as basal controls. PBMC were stimulated in vitro with anti-CD3/anti-CD28 monoclonal antibodies, and CD4+IFN- gamma+ or CD4+IL-10+ T cells were detected by flow cytometry intracellular staining. The frequency of peripheral CD4+IL-10+ T cells was significantly higher in STEMI patients as compared with controls. Conversely, the frequency of CD4+IFN-gamma+ T lymphocytes did not differ between STEMI and subjects without history of coronary artery disease. Six days after the revascularization procedure, the percentage of CD4+IL-10+ T cells was significantly decreased in BS but not in the PES group, whereas the relative percentage of CD4+IFN-gamma+ T lymphocytes were diminished in both groups as compared with baseline levels. Our data indicate that STEMI is associated with a peripheral expansion of CD4+IL-10+ T lymphocytes, and that primary coronary revascularization with implantation of either BS or PES is followed by a reduction in circulating CD4+IFN-gamma+ T lymphocytes. PES implantation, however, appears to inhibit the relative decrease of the IL-10 producing lymphocyte as observed in BS implanted patients, shifting the balance between pro-inflammatory and anti-inflammatory T cell populations in favor of the latter.     

Endogenous and adoptively transferred A-NK and T-LAK cells continuously accumulate within murine metastases up to 48 h after inoculation.

In murine models, therapeutic efficacy of adoptive immunotherapy (AIT) of cancer with lymphokine activated killer (LAK) cells is seen only when applied together with substantial doses of interleukin-2 (IL-2), probably because this cytokine is imperative for both motility and viability of the LAK cells.We wanted to investigate whether IL-2 in addition mediates an immunostimulatory activation and expansion of endogenous effector cells contributing to tumor regression. Using an immunoperoxidase technique, we have been able to longitudinally analyze the accumulation of tumor infiltrating lymphocytes expressing the pan-T cell/activated lymphocyte phenotype (Thy1.2), the natural killer (NK) cell phenotype (AsGM,) as well as the cytotoxic T (CD8) cell phenotype within experimental established B16 pulmonary melanoma metastases in C57BL/6 mice during the first 48 h after high dose IL-2 monotherapy. Whereas a substantial and selective infiltration of AsGM1+ lymphocytes in tumor tissue was seen (262 and 937 cells per sq.mm malignant tissue at 0 and 48 h, respectively), only a minor increase in accumulation of CD8+ cells was seen (106 and 171 cells per sq.mm tumor tissue at 0 and 48 h, respectively). The addition of adoptive transfer with lymphokine-activated adherent NK (A-NK) cells to the high-dose IL-2 treatment resulted in more than a 1.5 fold increase in infiltrating AsGM1+ cells compared to IL-2 therapy alone (1520 compared to 937 AsGM1+ cells per sq.mm malignant tissue). No substantial accumulation of CD8+ cells was observed in this setting either. In contrast, the treatment with high dose IL-2 together with adoptive transfer of mitogen-stimulated, lymphokine-activated T killer (T-LAK) cells increased the infiltration of CD8+ cells 10-fold compared to IL-2 monotherapy (2078 compared to 171 CD8+ cells per sq.mm malignant tissue, respectively). Interestingly, infiltration of both endogenous and exogenous cells continued over time, since the effector-to-tumor cell ratio in metastatic tissue dramatically increased from 1:8 and 1:6 at 16 h to 1:3 and 1:2 at 48 h after adoptive transfer of A-NK and T-LAK cells, respectively. These data underline the longevity of LAK cells in vivo and highlight the importance of IL-2 treatment in recruiting endogenous immune cells to tumor areas.     

替罗非班对经皮冠状动脉介入术患者免疫状态及炎性因子水平影响研究

目的：探讨替罗非班对经皮冠状动脉介入术患者免疫状态及炎性因子水平的影响。方法：选取2009年1月~2011年3月于本院进行经皮冠状动脉介入术治疗的78例急性心肌梗死患者为研究对象,将其随机分为对照组（常规用药组）39例和观察组（加用替罗非班组）39例,后将两组患者的总有效率及治疗前、治疗后24h及72h的外周血T淋巴细胞亚群、红细胞免疫功能及血清IL-6、IL-8、IL-10、CRP水平进行检测及比较。结果：观察组的总有效率高于对照组,血清IL-6、IL-8、IL-10、CRP、CD8^＋、红细胞免疫复合物花环率低于对照组,CD3^＋、CD4^＋、CD4^＋/CD8^＋、红细胞花环C3b受体花环率水平高于对照组,P均〈0.05,差异均有统计学意义。结论：替罗非班对经皮冠状动脉介入术患者免疫状态及炎性因子水平影响明显,肯定了其在本病治疗中的综合效果。     

慢性肾炎治疗前后血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群检测意义

目的探讨慢性肾炎患者治疗前后血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群的变化。方法分别应用放免法、ELISA法和单克隆抗体法对30例慢性肾炎患者治疗前后进行了血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群水平的检测,并与35名正常健康人作比较。结果慢性肾炎患者在治疗前血清IL-2和CD4/CD8比值明显低于正常人组（P〈0.05）,而IL-6、IL-10和IL-18水平高于正常人组（P〈0.01）;经半年治疗后血清IL-2、IL-6、IL-10、IL-18和CD4/CD8与治疗前组比较差异有统计学意义（P〈0.05）。结论检测慢性肾炎患者血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群水平对判断病情及其预后均具有一定的临床实用价值。     

An activated set point of T-cell and monocyte inflammatory networks in recent-onset schizophrenia patients involves both pro- and anti-inflammatory forces

We recently described a pro-inflammatory gene expression signature in the monocytes of 60% of patients with recent-onset schizophrenia (SCZ). Here we investigated whether the T-cell system is also in a pro-inflammatory state. A detailed fluorescence-activated cell sorting (FACS) analysis, e.g. of CD3+CD25+ T cells, IFN-γ+, IL-4+, IL-17A+ (CD4+) lymphocytes and CD4+CD25highFoxP3+ regulatory T cells, was performed on peripheral blood of 26 patients with recent-onset SCZ (in 19 of whom the inflammatory gene expression signature of the monocyte had been determined) and in age-/gender-matched healthy controls. Various relevant T-cell cytokines, e.g. sCD25, IFN-γ, IL-17A and IL-4, were measured in serum by a multiplex assay. We detected: (a) not only higher percentages of pro-inflammatory-prone monocytes, activated CD3+CD25+ T cells and pro-inflammatory Th17 cells in patients, but also higher percentages of anti-inflammatory CD4+CD25highFoxP3+ regulatory T cells and IL-4+ lymphocytes; (b) that this activated T-cell set point was reflected in significantly raised serum levels of sCD25; (c) that the up-regulation of IL-4+-containing lymphocytes was predominantly found in patients characterized by a monocyte pro-inflammatory set point; and (d) that regulatory T-cell and Th17-cell numbers were higher in patients irrespective of the pro-inflammatory state of the monocytes. Our data do not support the concept that the T-cell system is in a simple pro-inflammatory state in recent-onset SCZ, but do show that the monocyte and T-cell networks are activated and involve both pro- and anti-inflammatory forces. This suggests control within an activated inflammatory system.     

Th1／Th2／Th17 T／reg 对鉴别儿童支气管哮喘与单纯性肺炎的意义

目的：检测Th1（IFN-γ＋）、调节T细胞（CD2＋5Foxp3＋）、辅助T 细胞17（Th17）及细胞因子IL-2、IL -4、IL-17和IL-21在儿童支气管哮喘以及肺炎患儿外周血中的表达,探讨其在儿童支气管哮喘发病中的作用以及与肺炎鉴别的意义。方法收集哮喘患儿32例,肺炎患儿37例作为研究对象,选取同期体检的22例健康儿童为对照组。分离外周血单个核细胞,采用流式细胞术检测CD 4＋IFN-γ＋、CD 4＋CD 2＋5Foxp 3＋、CD4＋Th17＋细胞百分率。采用酶联免疫吸咐（ ELISA）法检测3组IL-2、IL-4、IL-17、IL-21表达水平。结果哮喘组患儿外周血CD4＋CD2＋5 Foxp3＋细胞占CD4＋T细胞的比率明显低于肺炎组及对照组;哮喘组患儿者外周血CD4＋IFN-γ＋细胞比例明显低于肺炎组和对照组;哮喘组患儿者外周血CD4＋Th17＋细胞所占比例高于肺炎组及对照组,差异均有统计学意义（ P ＜0*．05）。哮喘组患儿外周血IL-2的浓度明显低于对照组（ P ＜00．5）,肺炎组患儿外周血IL-2浓度明显低于对照组（ P ＜0．05）,但与哮喘组比较差异无统计学意义（ P ＞0．05）。哮喘组患儿外周血 IL4-、IL-17和IL-21的浓度明显低于肺炎组和对照组,差异有统计学意义（ P ＜0．05）。结论 Th1／T2h ／Th17／Treg功能失调参与了儿童支气管哮喘的发病机制,临床上可以通过检测Th1／Th2／Th17／Treg相关细胞因子的变化评估儿童支气管哮喘病情并辅助与肺炎的鉴别。     

布鲁氏菌病患者外周血树突状细胞、Th1／Th2细胞因子含量的检测及意义

目的：观察布鲁氏菌病患者外周血树突状细胞表型、Th1／Th2细胞含量的检测及意义。方法选取诊治的布鲁氏菌病患者50例为病例组,另选取同期进行健康体检的正常人50例作为正常组。采用Real time-PCR测定2组Th1相关转录因子T细胞表达的T盒（T-bet）、GATA连接蛋白3（GATA-3）、维A酸相关核孤儿受体γt（RORγt）、叉头蛋白3（Foxp3）及Th1／Th2细胞因子肿瘤坏死因子α（TNF-α）、干扰素γ（IFN-γ）、白介素6（IL-6）、白介素10（IL-10）mRNA含量。酶联免疫吸附实验（ELISA）测定TNF-α、IFN-γ、IL-6、IL-10蛋白表达及补体C3、C4含量。流式细胞术测定树突状细胞表型、Th1、Th2及T淋巴细胞亚群（ CD4＋T细胞、CD8＋T细胞、NK细胞）含量。结果病例组外周血Th1细胞相关转录因子T-bet、RORγt、Foxp3及Th1细胞、Th1／Th2、TNF-α、IFN-γ含量较对照组显著降低,Th2细胞及IL-6、IL-10含量较对照组显著升高,差异有统计学意义（ P ＜0．05）;病例组CD8＋3、CD8＋0、CD8＋6阳性的树突状细胞比例、CD4＋T细胞、NK细胞及补体C3、C4含量较对照组显著降低,CD8＋T细胞含量较对照组显著升高,差异有统计学意义（ P ＜0．05）;TNF-α、IFN-γ含量与CD4＋T细胞、NK细胞、C3、C4含量呈正相关性（ r值分别为2．879、3．214、3．255和2．978, P ＜0．05）,与CD8＋T细胞含量呈负相关性（ r值分别为－3．146和－3．011, P ＜0．05）。 IL-6、IL-10含量与CD4＋T细胞、NK细胞、C3、C4含量呈负相关性（ r值分别为－2．124、－2．343、－3．423、－2．789、－2．993、－2．566、－3．758, P ＜0．05）,与CD8＋T细胞含量呈正相关性（ r值分别为3．465、3．129, P ＜0．05）。结论布鲁氏菌病患者外周血成熟树突状细胞数目减少,同时Th1／Th2细胞及相关细胞因子失衡,且与机体天然免疫和细胞免疫功能降低有关,这可能在布鲁氏菌病发生发展过程中发挥重要作用。     

薏苡仁酯对高龄恶性肿瘤患者Treg细胞的影响

目的探讨薏苡仁酯(coixenolide)对高龄恶性肿瘤患者外周血调节性T细胞(Treg)的作用。方法检测30例80岁以上老年恶性肿瘤患者(肿瘤组)薏苡仁酯治疗前后外周血Treg占总CD4+T细胞比例和血IL-2mRNA变化。结果与30例健康老年人(对照组)进行比较。结果肿瘤组外周Treg占总CD4+T细胞比例、血IL-2mRNA水平均明显高于对照组(P<0.01)。肿瘤组外周血Treg比例与血IL-2mRNA水平呈正相关(r=0.921,P<0.01)。肿瘤组在薏苡仁酯治疗2个周期后,外周血Treg、IL-2mRNA水平较治疗前明显下降(P<0.01)。结论薏苡仁酯可能通过下调IL-2分泌和Treg数目解除免疫抑制而发挥抗肿瘤作用。     

氮网球花定碱盐酸盐对活化T细胞增殖的抑制作用

目的 探究氮网球花定碱盐酸盐(NMHC)抑制活化T细胞增殖的作用机制.方法 Ficoll法提取分离健康人外周血单个核细胞(PBMC),免疫磁珠法纯化出PBMC中的T细胞,抗人CD3/CD28抗体活化纯化后的静息T细胞.NMHC 0.125,0.5,2和8μmol·L-1与静息T细胞作用,用流式细胞术检测细胞毒性(96 ...