机采血小板在贮存过程中的形态和生化标志物动态改变

目的评估机采血小板在贮存期其形态及活化的生化标志物的改变,并探讨形态学计分与这些活化标志物之间的相关性。方法在光镜下观察机采血小板贮存0~5d时的形态变化,同时用商品试剂盒测定葡萄糖和乳酸浓度及可溶性P-选择素(sP-选择素)水平。结果贮存期间血小板形态学计分下降,而sP-选择素水平升高,两项指标存在负相关性(P0.05);葡萄糖浓度呈进行性下降,而乳酸浓度逐渐升高,但两者仅在第5天呈现一定相关性,但差异无统计学意义(P0.05);pH值在贮存第0天与最后1天比较出现显著下降(P0.05),在贮存第0、1、2、3天与血小板形态学计分呈显著正相关(P0.05),在第4天和第5天,这两项指标无相关性(P0.05)。结论机采血小板在贮存过程中形态和生化标志物发生动态改变,其中血小板形态学变化分值与sP-选择素呈负相关,与保存早期pH值呈正相关。     

储存机采血小板的凋亡对其聚集功能的影响

目的:探究机采血小板储存损伤对其凋亡影响及其存储期间聚集功能的变化,明确机采血小板的凋亡与其聚集功能的关系。方法:通过流式细胞术检测10例O型机采血小板在第2、5、8 d时的凋亡情况,同时分析其在第2、5、8 d时对ADP、Collagen、TRAP和ASPI激活剂诱导下聚集功能的变化。通过相关回顾分析探究血小板凋亡与其聚集功能的关系。结果:与对照组相比,储存机采血小板凋亡细胞百分比随时间的延长逐渐增高,第2、5、8 d凋亡细胞百分比分别为(2.87±0.31)%、(11.08±1.54)%和(27.99±2.76)%,随时间的延长其凋亡细胞百分比显著升高(F=14.32,P 0.01)。通过聚集功能分析发现,与储存d 2机采血小板相比,存储d 5的机采血小板对Collagen、TRAP和ASPI聚集功能显著减弱。与储存d 2和d 5的机采血小板相比,存储第8 d的机采血小板对Collagen、TRAP和ASPI诱导的聚集功能减弱更为显著(P 0.01);而其对ADP诱导的聚集功能减弱作用却无差异(P 0.05)。进一步相关和回归分析显示,机采血小板的凋亡情况与其聚集功能呈负相关(r=-0.9497,r=-0.9527,r=-0.9707,r=-0.9352),且关联性较强。结论:随着机采血小板储存时间的延长,其凋亡细胞比例逐渐升高,同时其聚集功能亦逐渐减弱,且其聚集功能的降低与其凋亡比例的升高密切相关。     

机采血小板储存过程中5种miRNA前体的表达变化

目的分析机采血小板储存过程中表达量有明显升高的5个miRNA前体(pre-miRNA)表达谱,为研究机采血小板中miRNA的成熟及调节机制提供参考。方法分别提取不同储存时间(1、3、5 d)各2 m L机采血小板总RNA,以5S rRNA为内参照,采用实时荧光定量(qRT-PCR)法分别检测5个miRNA前体pre-miR-16、-96、-150、-155和-316的表达水平。结果以新鲜机采血小板0 d pre-miRNA表达水平为基准,pre-miR-16的相对表达量在1、3、5 d分别为0.98±0.08、0.96±0.11、0.92±0.14;pre-miR-96的相对表达量在1、3、5 d分别为0.97±0.08、0.98±0.09、0.94±0.12;pre-miR-150的相对表达量在1、3、5 d分别为0.95±0.09、0.91±0.06、0.93±0.15;pre-miR-155的相对表达量在1、3、5 d分别为0.97±0.12、0.95±0.08、0.95±0.10;pre-miR-326的相对表达量在1、3、5 d分别为0.98±0.08、0.96±0.12、0.86±0.18。相对于新鲜血小板,上述5种pre-miRNA在储存过程中表达量无明显改变(P0.05)。结论机采血小板储存过程中表达量明显升高的5个miRNA的pre-miRNA表达相对稳定,并未伴随miRNA表达改变而改变。     

提高机采血小板血浆氧含量对血小板功能影响的探讨

为了探讨提高机采血小板血浆中的含氧量对血小板功能的影响,将机采血小板样品分为实验和对照2组。实验组在无菌条件下提高机采血小板血浆中含氧量(溶解氧)后,两组同时放置(22±2)℃水平振荡的血小板振荡仪器中保存。分别在血小板保存0、1、2、3、4、5天检测血小板量数量、血小板聚集反应功能、血小板液乳酸含量和血小板CD62p表达量。结果显示2组的血小板数量、血小板聚集反应功能均随保存时间延长而下降;2组间比较,血小板数量无显著性差异(P>0.05),血小板聚集反应功能实验组在保存2-3天明显好于对照组(P<0·05)。2组的血小板乳酸含量、血小板CD62p表达量均随保存时间延长而升高;2组间比较,实验组的血小板乳酸含量和血小板CD62p表达量在保存1-3天时低于对照组(P<0.05);机采血小板的含氧量在0天时实验组显著高于对照组(P<0.01)。结论提高血小板血浆中的含氧量(溶解氧)可弥补保存袋中血小板代谢的供氧不足,提高血小板的有氧代谢和血小板的保存质量。     

线粒体hsa-let-7b在机采血小板储存过程中的表达变化

目的通过检测机采血小板储存过程中线粒体hsa-let-7b的表达情况,探讨其对血小板凋亡可能的影响。方法提取不同储存时期机采血小板线粒体总RNA及miRNA,以5s RNA为内参照,利用实时荧光定量PCR(qRTPCR)分别检测hsa-let-7b及Bcl-x L mRNA的表达量变化并分析它们的相关性。结果在血小板贮存过程中线粒体hsa-let-7b表达水平明显增加,Bcl-x L mRNA在血小板贮存过程中呈下降趋势,2者之间的表达变化呈负相关,具有显著性(P0.05)。生物信息学分析推测let-7b可能靶向调控Bcl-x L。结论血小板线粒体hsa-let-7b的表达变化可能是下调Bcl-x L从而促进血小板凋亡的分子机制之一。     

健康献血者机采血小板储存中LncRNA LIPCAR 的表达及初步研究

目的 探讨健康献血者机采血小板伴随储存时间延长长链非编码RNA（long non-coding RNA，LncRNA）中预测心脏重塑的基因间长链非编码RNA（long intergenic noncoding RNA predicting cardiac remodeling，LIPCAR）的表达变化及意义。方法 收集陕西省血液中心2021 年11 月~2022 年7 月正常捐献者机采血小板32 份，于血小板专用22±2℃恒温振荡保存箱内保存，针对同一机采血小板样本通过延长储存时间（1，3，5，7 和9 天）检测血小板质量，使用实时荧光定量PCR（real-time quantitative PCR, qRT-PCR）检测LIPCAR 在不同储存时间的表达量，并分析其表达水平变化与捐献者性别和血型的关联性。结果 经检测血小板质量合格，在储存的第1，3，5，7 和9 天，伴随储存时间延长LIPCAR 表达量逐渐增高，数据总体差异有统计学意义（H=89.46，P ＜ 0.001）。对不同天数之间进行两两多重比较，第1 天和第5，7，9 天，第3 天和第7，9 天校正后的P 值均小于0.001；第5 天和第9 天校正后的P=0.016，差异均有统计学意义。不同储存天数LIPCAR 表达量根据性别分析，发现在第5 天和第1 天（t=2.189，P=0.038）、第7天和第1 天（t=2.320，P=0.028）、第9 天和第1 天（t=2.264，P=0.032）男性表达量高于女性，差异具有统计学意义；不同血型之间总体存在同质性差异（F=3.160，P=0.043），差异具有统计学意义。结论 经检测合格用于临床输血的机采血小板随着储存时间延长，第5，7 和9 天样本中LIPCAR 含量比第1 天明显增高，LIPCAR 对血小板储存条件敏感，有可能成为血小板储存损伤（platelet storage lesion，PSL）潜在的生物学标志物。     

冰冻机采血小板临床疗效分析

目的探讨冰冻机采血小板在临床上的疗效.方法对预防性输注冰冻机采血小板病人,检测血小板输注前和输后1h及24h血小板计数,计算出血小板增值(CCI);治疗性急性出血输注冰冻机采血小板病人检测血小板输注前及输后1h出血时间.以输注新鲜机采血小板的患者作平行对照.结果预防性输注组中,测定1h CCI＞7.5×109/L的百分率,冰冻机采血小板组86.7%(13/15),新鲜机采血小板组90.9%(20/22),两组比较差别无显著性(P>0.05);24h CCI＞4.5×109/L的百分率,冰冻机采血小板组53.3%(8/15),新鲜机采血小板组86.4%(19/22),两组比较差别有显著性(P<0.05);治疗性急性出血组输注血小板前两组出血时间无显著性差别(t=0,P＞0.05),输注后1h两组出血时间差别有显著性(P＜0.05).结论冰冻血小板治疗急性出血患者效果优于新鲜血小板,预防性输注效果显著差于新鲜血小板.     

冰冻机采血小板临床疗效分析

目的探讨冰冻机采血小板在临床上的疗效。方法对预防性输注冰冻机采血小板病人,检测血小板输注前和输后1h及24h血小板计数,计算出血小板增值(CCI);治疗性急性出血输注冰冻机采血小板病人检测血小板输注前及输后1h出血时间。以输注新鲜机采血小板的患者作平行对照。结果预防性输注组中,测定1hCCI>7.5×109/L的百分率,冰冻机采血小板组86.7%(13/15),新鲜机采血小板组90.9%(20/22),两组比较差别无显著性(P>0.05);24hCCI>4.5×109/L的百分率,冰冻机采血小板组53.3%(8/15),新鲜机采血小板组86.4%(19/22),两组比较差别有显著性(P<0.05);治疗性急性出血组输注血小板前两组出血时间无显著性差别(t=0,P>0.05),输注后1h两组出血时间差别有显著性(P<0.05)。结论冰冻血小板治疗急性出血患者效果优于新鲜血小板,预防性输注效果显著差于新鲜血小板。     

冰冻保存对机采血小板释放5-HT的影响

目的 观察冰冻保存对机采血小板释放5-HT 的影响.方法 常规以二甲基亚砜(DMSO)为冷冻保护剂、-80 ℃冰冻保存机采血小板30 d;采用全自动血细胞分析仪检测并比较冰冻保存前后血小板计数(PLT)、血小板平均体积(MPV)和血小板体积分布宽度(PDW);ELISA法检测冰冻前后及在阳离子没食子酸丙酯(C-PG)、凝血酶(thrombin,THB)、二磷酸腺苷(ADP)、胶原(Collagen)等不同PLT诱导剂激活作用下PLT释放5-HT的数量.结果 冰冻复苏后机采血小板计数变化不大(P>0.05),但MPV和PDW 均增加,血小板制品血浆5-HT含量也显著增高(P<0.01).在不同诱导剂作用下,新鲜血小板释放5-HT均高于冰冻保存的血小板,差异有显著性(P<0.01).结论 (1)机采血小板冰冻保存与解冻过程中,会引起血小板膜形态和生物活性改变.(2)冰冻保存后PLT对不同诱导剂的反应下降.     

储存血小板差异性长链非编码RNA表达谱研究

目的探讨机采血小板储存过程中差异性lncRNA表达谱变化及其差异表达的lncRNA在血小板储存损伤中潜在的生物学功能。方法收集13(人)份O型健康男性机采血小板各50 m L,于(22±2)℃下震荡储存,应用lncRNA芯片技术检测储存2、5、8 d机采血小板中lncRNA和mRNA表达谱变化;运用GO分析及KEGG分析预测差异表达的lncRNA功能分布,通过顺式调控分析预测lncRNA调控邻近蛋白编码基因表达的分子机制;采用实时PCR(RT-PCR)技术,验证8条lncRNA(MARCH2-2∶1、SLC2A2-1∶1、WBSCR16-3∶6、WEE1-2∶1、ENTPD6-2∶1、NKD2-1∶1、FOXS1-2∶1、MAPK13-3∶1)和4条mRNA(BCL2L1、CAPN2、MAPK14、VAMP8)的表达。结果芯片检测:血小板储存5与2 d相比,有162种lncRNA的表达量发生明显变化,表达升高的4种、降低的158种;8与2 d相比,有691种lncRNA表达量发生明显变化,表达升高的20种、降低的671种;8与5 d相比,246种lncRNA表达量发生明显变化,升高的2种,降低的244种。GO和KEGG分析:差异表达的lncRNA可能参与的生理过程有血小板激活、血小板聚集、内吞、凋亡、肌动蛋白纤维聚集、肌动蛋白骨架的调节;顺式调控分析:lncRNA具有调控血小板相关mRNA表达的功能,USP39-1调控VAMP8的表达、TMEM86B-2调控GP6的表达、FOXS1-2调控BCL2L1的表达等。RT-PCR验证:与芯片检测结果一致,其中lncRNA MARCH2-2∶1、WEE1-2∶1和NKD2-1∶1表达量升高,WBSCR16-3∶6、SLC2A2-1∶1、ENTPD6-2∶1、FOXS1-2∶1和MAPK13-3∶1表达量下降,BCL2L1,CAPN2,MAPK14和VAMP8的表达量也下降。结论血小板中含有大量的lncRNA,其表达量随着血小板储存时间延长而呈不同的变化趋势,总体来讲下调的lncRNA数量较多,且下调的幅度较大。部分变化的lncRNA可能与血小板生理功能密切相关,并且在血小板储存损伤中发挥作用。     

机采血小板保存期内血小板聚集功能和可溶性P-选择蛋白的变化

本研究探讨保存期内的机采血小板的聚集功能和可溶性P-选择蛋白含量的改变。收集20份机采血小板样品，分别在保存期第1天（0—24小时）、第2天（24—48小时）、第3天（48—72小时）、第4天（72—96小时）和第5天（96—120小时）检测血小板的聚集功能和可溶性P-选择蛋白含量。结果表明：以ADP作为诱导剂，保存期内机采血小板的血小板聚集功能降低明显，与第1天组比，组间差异均有统计学意义（P均〈0．01），第4天组的血小板最大聚集率≤3％；血浆可溶性P-选择素含量则随着保存时间的延长逐渐升高，与第1天组比，组间差异均有统计学意义（p均〈0．05）。结论：机采血小板在保存期内存在持续活化，且聚集功能下降明显，从第4天开始几乎已完全丧失对ADP的致聚反应，提示机采血小板的体外保存损伤应该引起高度重视。     

M-sol血小板保存液中添加花生四烯乙醇胺对体外保存血小板的影响

本研究探讨在M-sol（mixture of solutions）血小板保存液中添加花生四烯乙醇胺（N-arachidonoylethanolamine,ANA）对体外保存血小板的影响.利用Amicus血细胞分离机采集无偿献血志愿者的血小板,血小板保养液为M-sol,实验组加入终浓度为0.1-50 μmol/L的ANA,未加入ANA的另一组作为对照,置于22 ±2℃振荡仪中保存.于第7d取样,用MTT比色法分析血小板的存活率.选定最佳浓度的ANA加入血小板保存液中,分别于第1、5、7、9和11d取样,检测血小板计数（BPC）、平均血小板体积（MPV）、血小板分布宽度（PDW）、血小板磷脂酰丝氨酸（PS）膜外表达以及可溶性P-选择素（sP-selectin）含量.结果发现,加入终浓度为0.5μmol/L ANA的血小板存活率（91.23 ±5.44％）最高,与对照组（62.54±4.79％）相比,差异具有统计学意义（P＜0.05）;保存第1、5、7、9和11d实验组与对照组BPC均有下降趋势,但两组相比差较异均无统计学意义（P均＞0.05）;保存期间血小板MPV和PDW有增加趋势,实验组与对照组相比较差异均无统计学意义（P均＞0.05）.保存到第9和11d血小板膜外PS的表达,在实验组PS表达阳性率显著低于对照组（7.69±1.82％ vs 11.21±2.03％;10.74±1.78％ vs15.37±1.95％）,两组相比较差异具有统计学意义（P均＜0.05）;保存期间可溶性P-选择素含量在两组中均有增加趋势,实验组可溶性P-选择素含量显著低于对照组（30.19±2.03 ng/mL vs 39.18±2.66 ng/mL; 34.52±2.64ng/mL vs 43.23 ±2.58 ng/mL）,两组相比差较异具有统计学意义（P均＜0.05）.结论：将低浓度ANA加入血小板M-sol保存液中,在一定程度上可以减轻血小板贮存损伤.     

Phosphatidylserine exposure in platelet concentrates during the storage period: differences between the platelets collected with different cell separators.

BACKGROUND AND OBJECTIVES: Platelet alterations occur during the production and storage of platelet concentrates, the so called "storage lesion". We studied the platelet alterations during the storage period in apheresis concentrates, employing flow cytometry for phosphatidylserine (PS) detection on platelets during the five days of storage. MATERIAL AND METHODS: Twenty-seven single donor platelet concentrates harvested with the Cobe Trima, Baxter Amicus, or Haemonetics MCS+ were analyzed for PS exposure by flow cytometry on the day of production (day 1) and on days 3 and 5 of storage. Furthermore PS expression was analyzed in platelet donors' blood samples withdrawn before plateletpheresis. RESULTS: PS expression on platelets gave the following median values: in blood donors before apheresis it was 1.12% (0.13-1.78) in platelets concentrates on the first day (2 h after apheresis) 2.06% (0.66-15.2), the third day 6.57% (1.98-51.13) and the fifth day 23.04% (3.86-80.23). All differences between median values of PS expression in blood samples before apheresis, and platelets concentrates on days 1, 3 and 5 of storage, are statistically significant. The expression of PS in platelet concentrates was analyzed in relation to the blood cell separator used for the collection procedure and showed the following results: on day 1 the median values of PS in platelet concentrates collected with the three different blood cell separators, Trima, Cobe and MCS, did not show statistically significant differences. On day 3, the platelets concentrates collected with the Trima and with the MCS showed differences that were statistically significant. Those were respectively 10.59% (4.56-51.13) and 3.53% (1.98-12.61), p = 0.005. The PS expression in platelet concentrates collected with the Trima and MCS showed differences that are also statistically significant on day 5 at respectively 32.4% (9.61-80.23) and 8.57% (3.86-48.42), p = 0.005. CONCLUSIONS: PS exposure in platelet concentrates on days 3 and 5 rise to levels that could compromise the quality of the platelet units. Improvements in standardized platelet quality controls, and in platelet collection systems are required to reduce the storage lesions in platelets concentrates.     

In vitro properties of apheresis platelet during extended storage in plasma treated with anandamide

BackgroundIn China apheresis platelets (PLTs) are stored in plasma for only 5 days, resulting in PLT inventory pressures. Anandamide (ANA) was reported to be a potential agent to inhibit PLT apoptosis. The aim of this study was to evaluate the characteristics of extended storage PLTs in plasma treated with ANA in vitro.MethodsApheresis PLTs (n = 20) were prepared in plasma treated with ANA, and stored at 22 °C for up to 11 days. On day 1, 3, 5, 7, 9 and 11, PLTs were tested for PLT count, mean PLT volume (MPV), PLT distribution width (PDW), pH, pCO₂, pO₂, hypotonic shock response (HSR), phosphatidylserine (PS) exposure and soluble P-selectin content.ResultsPLTs stored in plasma with/without ANA didn't show significant differences during the first 5 days of storage. From the 7^th day on, PLTs stored in plasma with ANA displayed significantly lower PS expression, soluble P-selectin content and higher HSR scores than those stored in plasma without ANA (P <0.05), respectively.ConclusionThe extended storage of PLTs in plasma treated with 0.5 µmol/l ANA showed better characteristics of the PLTs, compared with the control group, which was suggested to potentially alleviate the PLT storage lesion.     

PLTs of blood group A1 donors express increased surface A antigen owing to apheresis and prolonged storage

BACKGROUND: In contrast to RBC transfusion, where ABO mismatch is potentially lethal, immunologic ABO matching has been considered less critical for PLTs. Nonetheless, PLTs bear ABO blood group antigens, some of them expressing very high levels. STUDY DESIGN AND METHODS: The expression of A antigen was investigated by flow cytometry on resting and stimulated human PLTs of 100 A and 10 O group donors, as well as on 17 PLT concentrates (PCs) after apheresis and daily during a 6-day storage, to determine possible changes in expression of A antigen on PLT surface. RESULTS: Considerable variation of A antigen expression on PLT surface of A1 group individuals was observed; A2 group PLTs could not be distinguished from O group PLTs. The variability of A antigen on A group PLTs also became evident on investigating PLT lysates by ELISA. A1 group PCs showed a significant increase of A antigen expression on their surface owing to apheresis (p = 0.001) and to storage (p = 0.0091). CONCLUSION: Apheresis and prolonged storage of A1 group PCs independently led to overexpression of A antigen on the PLT surface. This may make such PCs more susceptible to destruction by anti-A of O or B group recipients.     

Evaluation of platelets prepared by apheresis and stored for 5 days. In vitro and in vivo studies

To evaluate the effect of storage on apheresis platelets collected with a closed-system blood cell separator, an in vitro investigation was performed, with measurements of pH, lactate, ATP, the ratio of ATP to the total adenine nucleotide content, and adenylate kinase. Unmodified apheresis platelets and apheresis platelets with plasma added were compared with conventional platelets stored in PL-1240 or PL-732 plastic containers. During 6 days of storage, there were similar changes in all variables with one exception: the extracellular activity of adenylate kinase was lower in apheresis platelets with plasma than in the other three groups (p less than 0.01). In vivo studies were carried out with 111Indium-labeled autologous platelets in eight volunteers. Apheresis platelets with 100 mL of plasma added were stored in two 1000-mL containers (PL-732) at 22 degrees C during agitation. Platelets from one of the containers were labeled with 111Indium and transfused into the volunteer within 24 hours. Platelets from the other container were labeled after 5 days of storage and transfused into the same donor. There were no significant differences between apheresis platelets stored for 1 day and those stored for 5 days: the mean percentage of recovery was 58.4 and 57.6 percent, t1/2 was 69 and 67 hours, and the survival time was 5.5 and 5.6 days, respectively.     

血小板保养液与血浆混合保存血小板的初步研究

目的探讨血小板保养液与不同比例血浆混合保存单采血小板的效果。方法选用枸橼酸钠、醋酸钠、磷酸钠和氯化钠等组成血小板保养液,(22±2)℃振荡条件下保存单采血小板7d;按保养液与血浆混合比例,实验组分为实验Ⅰ组(50%保养液+50%血浆)和实验Ⅱ组(80%保养液+20%血浆),分别于1、3、5、7d取样检测血小板数(Plt)、pH值、葡萄糖消耗量、乳酸产生量和血小板膜CD62p的表达情况,并与100%血浆保存的血小板(对照组)比较。结果血小板保存到5d时,实验组与对照组比较Plt差异无统计学意义(P>0.05),其pH值较对照组均有明显下降(P<0.05),但pH仍>6.0;实验组血小板膜CD62p阳性表达率分别为32%和36%,比对照组的28%略高(P<0.05)。血小板保存到第7天时,Plt、pH和血小板膜CD62p阳性表达率,实验各组较对照组为差(P<0.05);1—7d葡萄糖平均消耗量实验Ⅱ组比对照组和实验Ⅰ组为高(P<0.05),而乳酸平均产生量则实验各组较对照组明显为高(P<0.05)。结论采用血小板保养液与20%或50%比例的血浆混合,短期(5d)保存单采血小板的pH值和血小板数量与用100%血浆保存单采血小板的效果基本相同,但保存在保养液与血浆混合液中的血小板更易激活。     

花生四烯乙醇胺对血小板相关凋亡蛋白的影响

目的在血站标准存储血小板条件下,探讨花生四烯乙醇胺(N-Arachidonoylethanolamine,ANA)对血小板细胞相关凋亡的影响。方法利用Amicus血细胞分离机从无偿献血志愿者采集血小板,向其中一组加入终浓度为0.5μmol/L的ANA为ANA组,未加入ANA的另一组作为对照组,置于(22±2)℃水平振荡的振荡仪保存7 d。采用流式细胞仪检测血小板磷脂酰丝氨酸(PS)膜外表达;ELISA检测可溶性P-选择素的释放,Western blot检测血小板caspase-3和caspase-9的表达以及免疫共沉淀分析BCL-XL和Bak蛋白相互作用。结果 ANA组PS表达阳性率显著低于对照组[(8.29±1.44)%vs.(14.24±2.47)%,P<0.05];ANA组可溶性P-选择素含量显著低于对照组[(75.08±6.35)ng/ml vs.(90.37±8.91)ng/ml,P<0.05];ANA组BCL-XL和Bak蛋白的结合量显著高于对照组,约为对照组的2.6倍(P<0.05);ANA组caspase-9活性形式的量占总量(包括caspase-9酶原形式和活性形式)的百分比显著低于对照组[(9.63±1.47)%vs.(23.24±2.47)%];ANA组caspase-3活性形式的量占总量(包括caspase-3酶原形式和活性形式)的百分比显著低于对照组[(6.3±1.4)%vs.(13.2±2.5)%],两组相比差异均具有统计学意义。结论 ANA促进BCL-XL和Bak蛋白的结合,抑制了caspase-3和caspase-9的活化,在一定程度上抑制血小板凋亡。     

The introduction of 7-day platelets: a university hospital experience

INTRODUCTION: Recently, the FDA approved the Post Approval Surveillance Study of Platelet Outcomes, Release Tested protocol which allows participating institutions to utilize 7 day platelets following guidelines. As one of the first hospitals to implement a 7-day protocol, we reviewed our hospital experience with 7-day Gambro apheresis platelets to determine the impact on inventory. METHODS: A review of apheresis platelet transfusions and outdate records was performed. Data were collected prospectively from March to August 2006. This data were compared with a retrospective review for the same time period in 2005. RESULTS: For the 1,503 platelets transfused from March-August 2005, the mean day of issue was 3.44 (SD = 1.060). During the same time period of 2006, 1,688 platelets were transfused with a mean day of issue of 4.02 (SD = 1.083). This difference was statistically significant (P < 0.001). The outdate rate dropped from 2.9% (44/1,547) to 1.3% (22/1,710, P < 0.001). During the study period, approximately 59.7% of the platelets were 7-day platelets. DISCUSSION: Over the 6-month period, we noted a decrease in outdates from 2.9% to 1.3%. There was a shift toward older platelets (from a mean of day 3.4 to day 4). During the study period, 139 platelets were transfused on days 6 or 7 of storage. Overall, the implementation of 7-day platelets in a university hospital setting was easily accomplished and has resulted in benefits to our institution by decreasing our outdate rate and to our patients by providing an additional 139 days 6 and 7 apheresis platelets with a potential cost savings of $78,952 (over the 6-month study).     

LncRNA TUG1通过调控microRNA-132-3p/SIRT1减轻脂多糖诱导的小肠上皮细胞损伤

目的:探讨LncRNA-TUG1在脂多糖（LPS）诱导的小肠黏膜上皮细胞损伤中的作用和机制。方法:使用LPS处理HIEC-6人类小肠黏膜上皮细胞24 h构建脓毒症损伤模型。全转录组RNA测序分析LPS处理后HIEC-6细胞中的mRNA、microRNA及lncRNA表达变化。实时荧光定量（qRT-PCR）及wester...