进展期胃癌HSP900α、CD44v6表达与其临床生物学行为关系的研究

目的:探讨HSP90α、CD44v6在进展期胃癌中的表达特点及其生物学行为,为进展期胃癌的临床治疗提供理论依据. 方法: 对93例进展期胃癌及其56例淋巴结转移癌中HSP90α、CD44v6的表达,应用免疫组化方法进行检测. 结果:HSP90α在胃癌及淋巴结转移癌中的阳性率分别为54.84% (51/93)、66.07%(37/56).HSP90α在胃癌中表达分别与癌细胞浸润深度、淋巴结转移及TNM分期有关(P<0.05); CD44v6在胃癌及淋巴结转移癌中的阳性率分别为50.54% (47/93)、73.21%(41/56),二者之间的差异显著(P<0.01). CD44v6在胃癌中的表达分别与癌细胞分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05). 结论:进展期胃癌中HSP90α、CD44v6阳性表达与其临床生物学行为尤其是淋巴结转移密切相关,患者可能预后较差,应采取积极的综合治疗措施.     

肝细胞癌CD44v6和p16基因蛋白的表达及其意义

目的探讨CD44v6和p16基因蛋白表达与肝细胞癌(HCC)转移和预后的关系。方法应用免疫组织化学方法,检测分析110例HCC组织中CD44v6及p116蛋白表达,结合随访资料分析。结果在HCC中,CD44v6和p16阳性表达率分别为42.7%和34.5%。CD44v6阳性表达的HCC转移率高(P<0.05),分化程度和患者>5年生存率低(P<0.05);p16阳性表达的HCC转移率低(P<0.05),分化程度和患者>5年生存率高(P<0.05)。CD44v6与p16表达呈负相关(r=-0.59,P<0.005)。结论CD44v6和p16表达与HCC转移和患者生存期密切相关,检测CD44v6和p16蛋白的表达可作为判断HCC预后的参考指标。     

胃癌组织中COX-2、CD44v6和MMP-9蛋白表达及意义

目的探讨胃癌组织中环氧化酶-2（COX-2）、CD44v6和MMP-9蛋白表达在胃癌发生、发展过程中的作用。方法采用免疫组化Envision法检测104例胃癌患者癌组织中COX-2、CD44v6和MMP-9蛋白表达,并与28份健康胃黏膜组织比较。结果胃癌组织中COX-2、CD44v6、MMP-9蛋白阳性表达率分别为57．7％（60／104）、59．6％（62／104）和63．5％（66／104）,正常胃组织中均呈低表达或不表达,两者有显著性差异（P〈0．001）;三者表达均与肿瘤浸润深度、临床分期及淋巴结转移呈明显正相关（P〈0．05）,而与患者性别、年龄及肿瘤分化程度无明显相关性;COX-2表达与CD44v6和MMP-9表达呈明显正相关。结论COX-2、CD44v6和MMP-9的过表达可促进胃癌的浸润、转移,三者具有正协同作用;COX-2、CD44v6和MMP-9联合检测可用于判断胃癌生物学行为。     

细胞粘附分子在胆囊癌中表达的意义

研究胆囊癌组织中细胞粘附分子CEA和CD44v6蛋白表达的意义。用免疫组化法检测 44例胆囊癌组织中CEA和CD44v6蛋白的表达。 44例胆囊组织中CEA和CD44v6蛋白表达的阳性率分别为 75 %和 48% ;两者的表达与肿瘤浸润外膜和淋巴结转移均呈正相关 (P <0 0 5 ) ;胆囊癌组织中CEA和CD44v6蛋白呈协同表达 (P<0 0 5 )。CEA和CD44v6蛋白与胆囊癌的发展和淋巴结转移密切相关。     

胃癌及淋巴结转移灶中CD44v6和上皮钙粘素的表达及其意义

目的 :探讨 CD4 4 v6和上皮钙粘素 (E- cd)在胃腺癌及淋巴结转移灶中的表达及其意义。方法 :应用免疫组化 SABC法检测 2 0例正常胃粘膜 ,2 0例异形增生 ,185例胃癌原发灶及 10 9例淋巴结转移灶中 CD4 4 v6和 E- cd表达。结果 :CD4 4 v6和 E- cd在异型增生、胃癌原发灶及淋巴结转移灶中阳性表达率分别为 2 0 .0 0 % (4/ 2 0 )、66.4 9% (12 3 / 185 )和87.18% (10 2 / 10 8)以及 85 .0 0 % (17/ 2 0 )、5 2 .97% (98/ 185 )和 2 6.5 0 % (3 1/ 117)。三者之间有显著差异 (P<0 .0 1)。CD4 4 v6表达率与胃癌生长方式、组织学类型、血管淋巴管内浸润及 TNM分期无关。 E- cd阳性表达与胃癌生长方式、组织学类型及 TNM分期显著相关。早期肠型胃癌 CD4 4 v6阳性表达率显著高于弥漫型 (P<0 .0 5 ) ,在进展期胃癌则无显著差异。E- cd的阳性表达率下降主要见于弥漫型胃癌。结论 :CD4 4 v6和 E- cd阳性表达同胃癌分化及生物学行为密切相关 ,是预测其侵袭转移有价值的指标。同时 ,此结果也支持胃癌两种不同起源的假说。     

进展期胃癌HSP90α、CD44v6表达与其临床生物学行为关系的研究

目的:探讨HSP90α、CD44v6在进展期胃癌中的表达特点及其生物学行为,为进展期胃癌的临床治疗提供理论依据.方法:对9 3例进展期胃癌及其5 6例淋巴结转移癌中HSP90α、CD44v6的表达,应用免疫组化方法进行检测.结果:HSP90α在胃癌及淋巴结转移癌中的阳性率分别为54.84%(51/93)、66.07%(37/56).HSP90α在胃癌中表达分别与癌细胞浸润深度、淋巴结转移及TNM分期有关(P＜0.0.5);CD44v6在胃癌及淋巴结转移癌中的阳性率分别为50.54%(47/93)、73.21%(41/56),二者之间的差异显著(P＜0.01).CD44v6在胃癌中的表达分别与癌细胞分化程度、浸润深度、淋巴结转移及TNM分期有关(P＜0.05).结论:进展期胃癌中HSP90α、CD44v6阳性表达与其临床生物学行为尤其是淋巴结转移密切相关,患者可能预后较差,应采取积极的综合治疗措施.     

整合素α2β1及CD44v4在胃癌组织中的表达及意义

目的讨论整合素α2β1和CD44v4在胃癌组织中的表达及意义。方法采用免疫组化SP法检测85例胃癌标本和50例癌旁正常胃组织中整合素α2β1和CD44v4的表达情况,分析二者的表达与胃癌生物学行为以及它们之间的相关性。结果①整合素α2β1与CD44v4在胃癌标本中的阳性表达率均高于癌旁正常组织(P<0.05)。②整合素α2β1和CD44v4在胃癌中的表达与胃癌的分化程度、TNM分期、淋巴结转移和浸润深度有关(P<0.05)。③整合素α2β1和CD44v4在胃癌中的表达呈正相关(r=0.517,P<0.05)。结论整合素α2β1和CD44v4在胃癌中高表达且在胃癌的浸润、转移过程中起正协同作用,并且与胃癌的分化程度、TNM分期、淋巴结转移和浸润深度密切相关,与患者的年龄、性别以及病理组织学类型无关。     

胃癌患者血清及癌组织中CD44v6表达及意义

[目的]检测胃癌患者血清及胃癌组织中CD44v6的表达及其与胃癌临床病理特征间的关系,进一步阐述CD44v6在胃癌发生发展中的作用。[方法]收集40例经病理证实的胃癌患者,20例正常健康体检者为对照组,20例取自距癌组织边缘5cm以上癌旁胃黏膜标本。采用双抗体夹心-酶联免疫吸附法检测40例胃癌患者术前和20例正常健康者血清中CD44v6水平,免疫组化SP法测定40例经手术切除的胃癌组织标本和20例癌旁胃黏膜标本中CD44v6的表达。[结果]①胃癌患者血清中CD44v6[(177.21±46.48)μg/L]高于正常健康者(P<0.01)。②癌组织中CD44v6的阳性表达率为77.5%,显著高于癌旁胃黏膜中的表达(P<0.01),CD44v6在血清和组织中的表达随组织学分级、临床分期的增高及有无淋巴结转移而升高,呈正相关性。[结论]CD44v6参与了胃癌的发生,并且可反映机体肿瘤的负荷状态及预测肿瘤的进展;检测CD44v6的血清含量可能为胃癌的诊断提供依据。     

黏附分子与老年人胃癌血行转移的关系

目的　研究黏附分子与老年人胃癌血行转移的关系及化疗对其影响。方法　应用流式细胞仪检测 56例老年胃癌患者手术前外周血CD44变异体 (CD44v5、CD44v6)表达水平 ,与健康老年人及非老年胃癌患者进行对照 ,并在应用化疗后观察黏附分子表达水平的变化。结果　老年胃癌组外周血CD44v5、CD44v6的表达水平分别为 1 6 73± 7 45、1 1 62± 5 1 2 ,明显高于健康对照组 (P <0 0 1 )。老年胃癌组肿瘤≥ 5cm、肿瘤浸润深度≥深肌层及淋巴结转移阳性患者外周血CD44v5、CD44v6的表达水平明显高于肿瘤 <5cm、肿瘤浸润深度 <深肌层及淋巴结转移阴性患者 (P<0 0 1 ,P <0 0 5 ,P <0 0 1 )。老年胃癌患者化疗后外周血CD44v5、CD44v6表达水平较化疗前明显下降 (P <0 0 1 )。结论　黏附分子具有介导老年人胃癌细胞血行转移的作用 ,化疗可降低老年胃癌患者外周血CD44v5、CD44v6的表达水平 ,具有抗黏附和肿瘤转移作用     

胃癌及癌前病变组织中CD44v6表达的意义

目的探讨CD44v6基因表达与胃癌发生及胃癌生物学行为的关系.方法应用抗CD44v6蛋白的单克隆抗体,采用免疫组化ABC方法对正常胃粘膜(n=10)、各级胃粘膜异型增生(轻度n=16,中度n=12,重度n=14)、早期胃癌(n=16)及进展期胃癌(n=52)进行研究,并与胃癌类型、大小、有无淋巴结转移等作了比较分析.结果正常胃粘膜CD44v6为阴性,随着胃粘膜病变的进展,CD44v6蛋白的表达率逐渐升高,至进展期胃癌,表达率达到顶峰.轻、中、重度异型增生表达率分别为12%,33%,43%;早期胃癌及进展期胃癌的表达率分别为44%和73%.各级异型增生表达率之间的差异无显著性,而进展期胃癌表达率显著高于早期胃癌(P＜0.05),淋巴结转移组的表达率显著高于淋巴结未转移组(82%vs56%,P＜0.05),肠型胃癌的表达率高于弥漫型胃癌(78%vs56%,P＜0.05),CD44v6蛋白的表达与胃癌肿块大小无相关性.结论胃粘膜重度异型增生在CD44v6基因表达上已具有明显的潜在恶性趋势,CD44v6基因表达阳性的胃癌具有更强的浸润及淋巴结转移的能力.     

Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer

AIM: To investigate the relationship between the expression levels of nm23 mRNA, CD44s, and CD44v6,and oncogenesis, development and metastasis of human gastric adenocarcinoma, colorectal adenocarcinoma,intraductal carcinoma of breast, and lung cancer.METHODS: Using tissue microarray by immuhistochemical (IHC) staining and in situ hybri-dization (ISH), we examined the expression levels of nm23mRNA, CD44s, and CD44v6 in 62 specimens of human gastric adenocarcinoma and 62 specimens of colorectal adenocarcinoma; the expression of CD44s and CD44v6in 120 specimens of intraductal carcinoma of breast and 20 specimens of normal breast tissue; the expression of nm23 mRNA in 72 specimens of human lung cancer and 23 specimens of normal tissue adjacent to cancer.RESULTS: The expression of nm23 mRNA in the tissues of gastric and colorectal adenocarcinoma was not significantly different from that in the normal tissues adjacent to cancer (P＞0.05), and was not associated with the invasion of tumor and the pathology grade of adenocarcinoma (P＞0.05). However, the expression of nm23 mRNA was correlated negatively to the lymph node metastasis of gastric and colorectal adenocarcinoma (r = -0.49, P＜0.01; r = -4.93, P＜0.01). The expression of CD44s in the tissues of gastric and colorectal adenocarcinoma was significantly different from that in the normal tissues adjacent to cancer (P＜0.05;P＜0.01). CD44v6 was expressed in the tissues of gastric and colorectal adenocarcinoma only, the expression of CD44v6 was significantly associated with the lymph node metastasis, invasion and pathological grade of the tumor (r = 0.47, P＜0.01; r = 5.04, P＜0.01). CD44sand CD44v6 were expressed in intraductal carcinoma of breast, the expression of CD44s and CD44v6 was significantly associated with lymph node metastases and invasion (P＜0.01). However, neither of them was expressed in the normal breast tissue. In addition, the expression of CD44v6 was closely related to the degree of cell differentiation of intraductal carcinoma of breast (x2= 5.68, P＜0.05). The expressional level of nm23mRNA was closely related to the degree of cell differentiation (P＜0.05) and lymph node metastasis (P＜0.01), but the expression of nm23 gene was not related to sex, age, and type of histological classification (P＞0.05).CONCLUSION: Patients with overexpression of CD44s and CD44v6 and low expression of nm23 mRNA have a higher lymph node metastatic rate and invasion. In addition, overexpression of CD44v6 is closely related to the degree of cell differentiation. Detection of the three genes is able to provide a reliable index to evaluate the invasion and metastasis of tumor cells.     

VEGF和CD44v6过表达与乳腺癌浸润转移的关系

目的检测血管内皮生长因子(VEGF)和转移相关黏附分子44v6(CD44v6)在乳腺癌中的表达情况,探讨两者与淋巴结转移的关系。方法采用免疫组化SP法检测92例乳腺浸润性癌中VEGF、CD44v6的表达情况。结果 VEGF在正常乳腺组织和乳腺癌中的阳性表达率分别为6.7%(3/45)和90.2%(83/92),且VEGF在淋巴结转移组中的阳性表达率(53/55)明显高于无淋巴结转移组(P<0.05)。CD44v6在正常乳腺组织及乳腺癌中的阳性表达率分别为8.9%(4/45)和85.9%(79/92),而且CD44v6在淋巴结转移组中的阳性表达率(51/55)明显高于无淋巴结转移组(P<0.05)。VEGF和CD44v6表达呈正相关关系(r=0.497,P<0.05)。结论 VEGF、CD44v6在乳腺癌组织中高表达,且均与淋巴结转移有关(P<0.05),两者可能在乳腺癌的远处转移中起协同作用。     

Expression of vascular endothelial growth factor C and chemokine receptor CCR7 in gastric carcinoma and their values in predicting lymph node metastasis

AIM: To study the expression of vascular endothelial growth factor C (VEGF-C) and chemokine receptor CCR7 in gastric carcinoma and to investigate their associations with lymph node metastasis of gastric carcinoma and their values in predicting lymph node metastasis. METHODS: The expression of VEGF-C and CCR7 in gastric carcinoma tissues obtained from 118 patients who underwent curative gastrectomy was examined by immunohistochemistry. Among these patients, 39 patients underwent multi-slice spiral CT (MSCT) examination. RESULTS: VEGF-C and CCR7 were positively expressed in 52.5 and 53.4% of patients. VEGF-C expression was more frequently found in tumors with lymph node metastasis than those without it (P<0.001). VEGF-C expression was also closely related to lymphatic invasion (P<0.001), vascular invasion (P<0.01), and TNM stage (P<0.001). However, there was no significant correlation between VEGF-C expression and age at surgery, gender, tumor size, tumor location, Lauren classification, and depth of invasion. CCR7 expression was significantly higher in patients with lymph node metastasis compared with those without lymph node metastasis (P<0.001) and was also associated with tumor size (P<0.01), depth of invasion (P<0.001), lymphatic invasion (P<0.001), and TNM stage (P<0.001). However, the presence of CCR7 had no correlation to age at surgery, gender, tumor location, Lauren classification, and vascular invasion. Among the 39 patients who underwent MSCT examination, only CCR7 expression was related to lymph node metastasis determined by MSCT (P<0.05). In the current retrospective study, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of VEGF-C and CCR7 expression in the diagnosis of lymph node metastasis for patients with gastric carcinoma were 73.8%, 70.2%, 72.6%, 71.4% and 72.0%, and 82.0%, 77.2%, 79.4%, 80.0% and 79.7%, respectively. After subdivision according to the combination of VEGF-C and CCR7 expression, receiver operating characteristic (ROC) analysis showed that the accuracy of the combined examination of VEGF-C and CCR7 expression in predicting lymph node metastasis was relatively high (area under ROC curve [Az]=0.83). CONCLUSION: The expression of VEGF-C and CCR7 is related to lymph node metastasis of gastric carcinoma and both of them may become new targets for the treatment of gastric carcinoma. Furthermore, the combined examination of VEGF-C and CCR7 expression in endoscopic biopsy specimens may be useful in predicting lymph node metastasis of gastric carcinoma and deciding the extent of surgical lymph node resection.     

Expression of human chorionic gonadotropin, CD44v6 and CD44v4/5 in esophageal squamous cell carcinoma

AIM: To study the relationship between the expression of human chorionic gonadotropin (HCG), CD44v6, CD44v4/5 and the infiltration, metastasis of esophageal squamous cell carcinoma. METHODS: By labeled streptavidin-biotin technique, the expressions of HCG, CD44v6, and CD44v4/5 in 42 patients with esophageal squamous cell carcinoma were examined. RESULTS: The positive rate of HCG expression in patients with lymph node metastasis was 85.71% (18/21), higher than that (57.14%, 12/21) in those without lymph node metastasis (P<0.05). The positive rate of CD44v6 expression was 71.43% (15/21) in lymph node metastasis group, and 38.09% (8/21) in non-metastasis group; there was a significant difference between the two groups (P<0.05). The positive rate of CD44v4/5 expression was 76.19% (16/21) in lymph node metastasis group, and 42.86% (9/21) in non-metastasis group; there was also a significant difference between them (P<0.05). From grade Ⅰ to grade Ⅲ in differentiation, the positive rate of HCG expression was 84.62% (11/13), 70.59% (12/17) and 58.33% (7/12), respectively; there was no significant difference among them (P<0.05). The positive rate of CD44v6 expression in grades Ⅰ-Ⅲ of cancer tissues was 76.92% (10/13), 52.94% (9/17), and 33.33% (4/12) respectively; there was no significant difference among them. The positive rate of CD44v4/5 expression in grades Ⅰ-Ⅲ of cancer tissues was 69.23% (9/13), 64.71% (11/17), and 41.67% (5/12) respectively; there was no significant difference among the three groups. There was no correlation between the positive rates of HCG and CD44v6, CD44v4/5 expression. Cancer cells in carcinomatous emboli and those infiltrating into vascular wall strongly expressed HCG, CD44v6, and CD44v4/5. CONCLUSION: Expression of HCG, CD44v6, and CD44v4/5 in esophageal squamous cell carcinoma is related to its infiltration and metastasis. HCG, CD44v6, and CD44v4/5 have different effects on the infiltration and metastasis of esophageal squamous cell carcinoma.     

CD44v6在胰腺癌组织中的表达及其临床意义

目的：探讨CD44v6蛋白表达与胰腺癌增殖及淋巴结转移的关系。方法：应用免疫组织化学（S－P）法检测38例胰腺癌组织标本中CD44v6和PCNA的表达。结果：CD44v6在胰腺癌中的阳性率为65％（25／38），与淋巴结转移呈非常显相关（P＜0．01），与胰分化程度无关（P＞0．05）。CD44v6阳性染色胰腺组织中PCNA标记率较阴性高（P＜0．05）。结论：CD44v6在胰腺癌的增殖，转移过程中可能起重要作用，可作为胰腺癌预后预测的生物学指标之一。     

胃癌中CD15s抗原和CD44v6基因蛋白的表达及其意义

[目的 ]探讨唾液酸化的路易斯寡糖 X(CD15s)抗原和CD4 4v6基因蛋白表达与胃癌临床病理指标和患者预后的关系。 [方法 ]应用高敏感性的催化信号放大免疫组化技术 ,对早期胃癌 17例、中期胃癌 2 1例和晚期胃癌5 7例组织进行CD15s抗原和CD4 4v6蛋白检测 ,并结合肿瘤的病理学指标和临床随访资料进行分析。 [结果 ]在胃癌中 ,CD15s和CD4 4v6的表达阳性率分别为 86 .3%和 82 .1%。且晚期胃癌明显高于早、中期胃癌 (P <0 .0 5 )。CD15s和CD4 4v6表达水平具有一致性 ,均与胃癌浆膜浸润、淋巴结转移和患者预后呈正相关 (P <0 .0 5 )。 [结论 ]CD15s和CD4 4v6表达与胃癌转移和患者生存期密切相关 ,检测CD15s和CD4 4v6的表达可作为判断胃癌预后的参考指标。