烧伤后血,尿Tamm—Horsfall蛋白变化的意义

本文用放免法测定59例严重烧伤病人血尿THP含量并进行动态观察,发现血、尿THP含量明显降低,降低的程度与烧伤严重程度和肾功能损害程度密切相关,与BUN、Cr呈显著负相关,与Ccr呈显著正相关。血、尿THP具有变化幅度大,发生早,组织学分型的血、尿THP对严重烧伤后肾脏髓袢及远曲小管损害具有预警意义。     

几种提纯尿Tamm—Horsfall蛋白的方法比较

The measurements of serum, urine Tamm-Horsfall protein (THP) and its antibody are essential to the early diagnosis of tubulointerstitial diseases and acute rejection after renal transplantation. The purification of THP proves to be the key procedure for the production of diagnostic kit. Once, twice and three times salt precipitation with 0.58 mol/L NaCl and chromatography with Sephadax G-200 were adopted to purify urine THP. Enzyme immunoassay was used to detect the antigenicity of the products, SDS-PAGE to determine the molecular weight (MW) and the electrophoretic purity (EP), Beckman DU-7 model spectrophotometer to scan the ultraviolet absorption spectrum. Evaluation of the four methods was based on the purity and the antigenicity of the produced THP, the output, the complexity of procedure, and the cost of required materials. Results show that the MW of THP is 93.0 kd; the ultraviolet absorption maximum is at lambda = 278 nm; the outputs are 7.5, 5.5, 3.8, and 0.4 mg/L; the concentrations of THP with antigenicity are 65%, 75%, 98% and 100%, respectively, and the EPs of three times salt precipitation and chromatography are better. It is concluded that three times salt precipitation should be recommended because of its excellence in purity and antigenicity of the produced THP, the simplicity of manipulation, and the cheapness of the materials needed.     

草酸钙肾结石大鼠肾组织 Tamm －Horsfall 蛋白表达及温肾化石汤调节

目的：研究草酸钙肾结石大鼠肾组织TH蛋白的表达,并用中药温肾化石汤调节,探讨该方防治肾结石复发的作用机制。方法：雄性SD大鼠60只,随机分为对照组、肾结石组、温肾化石汤组,每组各20只,肾结石组和温肾化石汤组以含有0．5％的乙二醇和0．5％的氯化铵的混合溶液致石剂代替饮用水,温肾化石汤组同时每日灌服中药温肾化石汤,二周后处死大鼠,解剖观察肾脏剖面结石形成,并用免疫组织化学法测定肾组织TH蛋白的相对表达量。结果：肾结石组肾脏成石程度最重,与温肾化石汤组比差异显著,对照组肾组织TH蛋白表达最强,其次为温肾化石汤组,肾结石组,三组相比差异显著。结论：TH蛋白可以抑制肾结石形成,中药温肾化石汤可以恢复大鼠肾组织TH蛋白表达量,推测此可能为该方防治肾结石复发的重要作用机制。     

尿毒症患者血浆纤维结合蛋白及Tamm—Horsfall蛋白测定

作者对10例尿毒症患者测定其血浆纤维结合蛋白(Fn),Tamm-Horsffall蛋白(THP)等指标。结果示尿毒症患者Fn及THP均明显低于正常值(P<0.01及0.005),且与血肌酐浓度呈负相关。提示尿毒症患者肾功能严重受损,失去调节Fn的功能。此外,由于肾小管的显著减少致使THP合成降低。对尿毒症患者测定Fn和THP有助于了解患者的肾功能状态和机体抗感染等免疫功能。     

尿Tamm－Horsfall蛋白测定及正常值调查

尿Ｔａｍｍ－Ｈｏｒｓｆａｌｌ蛋白测定及正常值调查江苏职工医科大学姚婷，朱新侠Ｔａｍｍ－Ｈｏｒｓｆａｌｌ蛋白（ＴＨＰ）是一种大分子糖蛋白，是由肾小管髓袢升支原壁段及远曲小管上皮细胞合成。肾脏ＴＨＰ含量比其它组织高１００倍［２］。正常人尿中ＴＨＰ含量较为...     

内皮素在大鼠药物性肾损害尿液中的变化及意义

目的 :探讨尿内皮素 - 1(ET - 1)在大鼠药物性肾损害中的变化及意义。方法 :采用庆大霉素制造动物模型、观察庆大霉素致大鼠急性肾损伤的表现 ,观察尿ET - 1、尿糖、尿钠、2 4h尿蛋白定量等指标变化。结果 :肾损伤组尿ET - 1含量进行性升高 ,与正常对照组差异有显著性 (P <0 .0 1)。结论 :尿ET - 1参与了肾毒性肾损伤的发生与发展 ,尿ET - 1含量进行性升高显示肾损伤程度加重。     

尿五种蛋白RIA评估原发性肾脏病早期肾损害

尿五种蛋白ＲＩＡ评估原发性肾脏病早期肾损害张皓１宣一江１朱继华２陈忠良１吴炜１李淮岗１为探讨尿五种微量蛋白放免测定对肾损害诊断价值。本文通过对５０例正常人及８３例原发性肾病（ＳＫ）患者尿液：α１－微球蛋白（α１－ＭＧ）、β２－微球蛋白（β２－ＭＧ）、...