16层螺旋CTA对颅内动脉瘤的诊断价值及术后评价

目的:探讨16层螺旋CTA对颅内动脉瘤的诊断价值,以及开颅夹闭术后随访中的应用价值。方法:回顾性分析经手术及经动脉数字减影血管造影(digital subtraction angiography,DSA)证实的颅内动脉瘤患者38例,所有病例采用16层螺旋CTA检查,运用容积重建和最大密度投影两种后处理技术评价动脉瘤的大小、形态、位置及周围结构的关系,观察动脉瘤夹闭术后的显示情况。结果:本组38例患者,CTA检出42个动脉瘤,其中2例为2个动脉瘤,1例为3个动脉瘤。后交通动脉瘤20例,前交通动脉瘤10例,大脑中动脉瘤6例,颈内动脉颅内段动脉瘤5例,椎动脉瘤1例。其中囊状动脉瘤28个,梭形动脉瘤8个,不规则状动脉瘤6个,直径约2～23 mm,平均直径约为5.8 mm。CTA能清晰显示动脉瘤夹闭术后情况。结论:16层螺旋CTA能及时准确诊断颅内动脉瘤,能够为临床提供重要信息,可作为动脉瘤夹闭术后病例随访的首要检查方法。     

Application of 16-slice spiral CT angiography in diagnosis and post-operative evaluation of intracranial aneurysms

目的:探讨16层螺旋CTA对颅内动脉瘤的诊断价值,以及开颅夹闭术后随访中的应用价值.方法:回顾性分析经手术及经动脉数字减影血管造影(digital subtraction angiography,DSA)证实的颅内动脉瘤患者38例,所有病例采用16层螺旋CTA检查,运用容积重建和最大密度投影两种后处理技术评价动脉瘤的大小、形态、位置及周围结构的关系,观察动脉瘤夹闭术后的显示情况.结果:本组38例患者,CTA检出42个动脉瘤,其中2例为2个动脉瘤,1例为3个动脉瘤.后交通动脉瘤20例,前交通动脉瘤10例,大脑中动脉瘤6例,颈内动脉颅内段动脉瘤5例,椎动脉瘤1例.其中囊状动脉瘤28个,梭形动脉瘤8个,不规则状动脉瘤6个,直径约2 ～23 mm,平均直径约为5.8 mm.CTA能清晰显示动脉瘤夹闭术后情况.结论:16层螺旋CTA能及时准确诊断颅内动脉瘤,能够为临床提供重要信息,可作为动脉瘤夹闭术后病例随访的首要检查方法.     

64排CTA对颅内破裂动脉瘤早期诊断价值的研究

目的：探讨64排CTA对颅内破裂动脉瘤早期诊断的应用价值。方法：将86例急诊入院、发病时间在72h内的自发性蛛网膜下腔出血患者进行64排CTA检查,并根据其结果决定是否早期行开颅动脉瘤夹闭术或血管内介入治疗。结果：86例自发性蛛网膜下腔出血的患者中,有81例患者颅脑CTA检查发现颅内动脉瘤,其中单发动脉瘤64例,多发动脉瘤（2个及以上）17例;颅内动脉瘤夹闭术后CTA复查发现漏诊动脉瘤1例,为大脑前动脉A3段动脉瘤。有3例首次颅脑CTA检查结果阴性,再次行DSA检查,其中发现颈内后交通动脉瘤2例,前交通动脉瘤1例,DSA检查结果阴性2例。结论：CTA可以作为颅内动脉瘤首选检查方法,可根据其结果对颅内动脉瘤进行早期治疗。在绝大多数自发性蛛网膜下腔出血的诊断和处理中高质量的颅脑CTA可替代DSA,快捷、无创的颅脑CTA有助于自发性蛛网膜下腔的早期诊断和早期处理的完成。     

64排CTA对颅内动脉瘤诊断价值研究

目的探讨64排CTA对颅内动脉瘤的诊断价值及其对临床治疗的指导作用。方法回顾分析55例自发性蛛网下腔出血病人,经CTA初步检查证实颅内动脉瘤后,行手术治疗或DSA检查。分析各项影像学指标与手术和（或）DSA所见之间的关系。结果 55例患者中检出动脉瘤61个,其中47例患者行开颅手术夹闭动脉瘤,8例患者行介入手术。CTA显示瘤体、瘤颈、载瘤动脉和周围血管及颅骨的关系清晰、确切,与术中符合率100%。30例患者术后复查CTA,3例夹闭不全,其中1例再次出血。结论因颅内动脉瘤导致蛛网膜下腔出血患者,64排CTA具有敏感性高、快速、无创特点,可以作为诊断颅内动脉瘤的首选影像学诊断方法。并可指导手术及介入治疗。     

CT血管造影对颅内动脉瘤术后夹闭不全的评估价值

目的研究CT血管造影(CTA)对颅内动脉瘤术后夹闭不全的评估价值。方法收集我院2015年12月-2017年11月间收治的45例颅内动脉瘤行开颅夹闭动脉瘤术治疗的患者,术后均行CTA检查,分析CTA评估颅内动脉瘤术后闭夹不全的临床价值。结果所有患者均于术后7-15d进行CTA复诊,1例发现新生动脉瘤1枚,再次手术给予夹闭;2例出现夹闭不全,再次手术调整瘤夹位置;5例发现脑积水,行脑室腹腔分流术进行处理;阅片结果除1例图像质量显示不佳外,其余均可清晰显示载瘤动脉与动脉瘤夹间的关系、动脉夹数量与位置、载瘤动脉通畅与否、动脉瘤颈残留、脑血管痉挛等信息,术后3个月患者均回院复诊,均显示颅内情况良好。结论CTA检查具有无创、检查时间短、费用低等特点,可准确显示动脉瘤夹闭术后颅内血管、脑实质等情况,在颅内动脉瘤夹闭术后具有较高的应用价值。     

64层CT血管造影在颅内动脉瘤诊断及术后评价中的应用

目的 探讨64层螺旋CT血管造影(computed tomography angiography,CTA)对颅内动脉瘤的诊断价值,以及对动脉瘤夹闭术后评价的价值.方法 回顾性分析25例临床拟诊为颅内动脉瘤的临床资料,患者均进行了64层CTA检查,其中21例行手术治疗,术后再行CTA复查,比较两次CTA结果,评价动脉瘤夹闭情况.结果 在25例患者中64层螺旋CTA共发现21例25个动脉瘤,CTA可清楚显示动脉瘤位置、大小、形态及与周围结构的关系,与手术所见一致(符合率100%).动脉瘤夹闭术后CTA能够清楚显示动脉瘤体消失,显示载瘤动脉与动脉瘤夹之间关系及动脉瘤夹的数量和位置.结论 64层螺旋CTA在诊断颅内动脉瘤及动脉瘤术后随访中具有重要临床价值.     

多排螺旋CT血管成像在脑动脉瘤诊断中的应用

目的探讨CT血管造影(CTA)在颅内动脉瘤诊断中的应用价值。方法对22例因蛛网膜下腔出血怀疑动脉瘤破裂病人行CTA检查,分别用后处理软件VR、MIP、MPR进行成像,并与同期进行的DSA检查8例图像及1例手术情况进行对比。结果22例CTA共发现动脉瘤25个,其中大脑前动脉瘤4例,大脑中动脉瘤5例,前交通动脉瘤4例,后交通动脉瘤3例,颈内动脉瘤7例。3例为多发动脉瘤。DSA共发现9个动脉瘤,7例与CTA相符。1例患者行动脉瘤手术夹闭。结论CTA用于脑动脉瘤诊断,快速准确无创,具有较高的临床应用价值。     

吲哚菁绿荧光血管造影在颅内动脉瘤手术中的临床研究

目的探讨吲哚箐绿荧光血管造影治疗颅内动脉瘤的应用价值。方法回顾性总结10例10个颅内动脉瘤。术中采用吲哚箐绿荧光血管造影,评估动脉瘤夹闭情况以及载瘤动脉的血供情况,根据造影结果必要时调整动脉瘤夹。术后行CT血管显像（CTA）判断动脉瘤夹闭情况。结果术中荧光血管造影发现动脉瘤残颈2例,载瘤动脉狭窄1例,均根据造影结果及时调整动脉瘤夹。术后CTA检查证实动脉瘤夹闭完全。结论显微镜联合吲哚菁绿荧光血管造影是动脉瘤手术中的有用工具,为动脉瘤夹闭术中提供实时信息,及时检查手术效果,可有效减少术后并发症,提高手术的安全性,从而能有效改善患者预后。     

3D-CTA在颅内动脉瘤诊治中的价值

目的:探讨三维CT血管造影(Three dimensional-computed tomography angiography,3D-CTA)在颅内动脉瘤病因诊断和治疗中的临床价值.方法:对2007年9月至2008年8月就诊的115例自发性蛛网膜下腔出血患者先进行头部3D-CTA检查.后进行DSA检查,检出动脉瘤89例,动脉瘤瘤颈夹闭术(81例)和介入栓塞术(8例),行手术和血管介入患者1周后全部行CTA复查,比较术前3D-CTA与DSA结果.结果:89例患者CTA枪出颅内动脉瘤97个,DSA检出颅内动脉瘤93个.其中5例CTA发现并经手术证实的前交通动脉小动脉瘤和3例后交通动脉小动脉瘤DSA检查为阴性;4例CTA检查阴性,DSA检查发现颅内动脉瘤并经手术证实,3D-CTA对颅内动脉瘤病因诊断的敏感性为95.6%,特异性为100%,准确性为96%,与DSA或/和手术诊断符合率为88.1%.结论:3D-CTA诊断结果与2D-DSA或/和手术诊断符合率较高,应灵活地结合CTA和DSA检查对颅内动脉瘤达到最大稗度上的正确诊断.     

颅内破裂微小动脉瘤的急性期显微手术治疗

目的总结颅内破裂微小动脉瘤的急性期显微手术的临床经验。方法 12例自发蛛网膜下腔出血患者经CTA或DSA确诊为颅内微小破裂动脉瘤,其中大脑中动脉动脉瘤5例,大脑前动脉动脉瘤3例,前交通动脉瘤4例,hunt分级Ⅰ~Ⅲ级10例,Ⅳ级2例。在发病3d内行显微手术夹闭,其中6例手术经额锁孔入路完成。术后随访半年。结果 12例动脉瘤均顺利夹闭,8例术后复查头部CTA显示夹闭满意,所有患者随访均无再出血。结论显微手术是治疗颅内微小动脉瘤的重要方式,急性期手术及个体化选择锁孔手术对减少再出血几率、减小损伤具有重要意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.