双波长薄层扫描法测定益母口服液中水苏碱的含量

采用双波长薄层扫描法测定了益母口服液中水苏碱的含量，用岛津ＣＳ－９３０型扫描仪于λ＿ｓ＝５１０ｎｍ，λ＿Ｒ＝７００ｎｍ波长处扫描，随行外标两点法计算含量。线性范围为１～１０μｇ，回归方程Ａ＝２９７．６０Ｃ－４９１．８０，ｒ＝０．９９９５。回收率为９６．６１％，ＲＳＤ＝２．６５％（ｎ＝５）。     

双波长薄层扫描法测定优力藤片中雷藤素甲的含量

用双波长薄层扫描法测定优力藤片（ＹＯＬＩＴＥＮ）中雷藤素甲的含量。采用硅胶Ｇ－ＣＭＣ Ｎａ薄板，样品经氧化铝吸附、氯仿回流提取、浓缩后点样。展开剂为乙酸乙酯－氯仿（１．３：２．０），用岛津ＣＳ－９０００型扫描仪于λｓ＝５１０ｎｍ，λＲ＝６１０ｎｍ波长处扫描。随行外标两点法计算含量。线性范围为０．１２３１～０．７３８６μｇ，回归方程Ａ＝６１８０３．２６Ｃ＋５５７６．８５，ｒ＝０．９９９８。回收率为１     

薄层扫描法测定芍黄片中芍药甙的含量

用正丁醇提取，薄层扫描法测定芍黄片中芍药甙的含量，薄层条件为含０．５ＣＭＣ－Ｎａ和硅胶ＧＦ２５４的薄层板，氯仿－甲醇－醋酸乙酯（８：３：１）为展开剂，扫描测定λｓ＝２１５ｎｍ，λＲ＝２９０ｎｍ，平均回收率为１００．２５％，ＲＳＤ为２．２７％。     

高效薄层扫描法测定四物汤中阿魏酸含量

用高效薄层扫描法（ＨＰＴＬＣ）测定了中药复方四物汤群煎液、分煎混合液、当归和川芎单煎液中阿魏酸的含量。以高效硅胶薄层板，苯－氯仿－乙酸乙酯－甲酸（８∶２∶２∶０．５）为展开剂，在紫外灯３６５ｎｍ波长下定位，用ＣＳ－９３０型薄层扫描仪进行测定，λｓ＝３２０ｎｍ，λＲ＝２４０ｎｍ，狭缝１．２ｍｍ×１．２ｍｍ，线形化参数：ＳＸ＝７，反射法锯齿形扫描，二点外标法定量测定     

小蔓长春花组织培养物中长春胺的含量测定

采用薄层扫描法测定小蔓长春花组织培养物中长春胺的含量。应用高效硅胶Ｇ薄层板，展开剂为石油醚－氯仿－甲醇（１０：１０：１．２）。在λＳ＝４９０ｎｍ，λＲ＝５５０ｎｍ波长处扫描。长春胺标准品的线性范围为０．５３５￣３．７４５μｇ；平均回收率为９８．６８％，ＲＳＤ＝１．９％（ｎ＝６）。小蔓长春花组织培养物经碱性氯仿和２％硫酸提取后点样，按随行外标两点法进行测定，计算样品的含量。测得在固体和液体培养基Ｂ５     

用薄层色谱法测定骨康片中士的宁和马钱子碱含量

目的 建立骨康片中士的宁和马钱子碱的含量测定方法。方法 采用双波长薄层扫描法,硅胶ＧＦ２５４薄层板,展开剂为甲苯－丙酮－乙醇－浓氨试液（４：３：１：２）,检测波长,士的宁λｓ＝２５８ｎｍ,λＲ＝３１０ｎｍ;马钱子碱λＳ＝３０１ｎｍ,λＲ＝３３５ｎｍ。结果 士的宁和马钱子碱的相关系数分别为０．９９９６和０．９９９９,方法的回收率分别为９９．８３％（ＲＳＤ＝１．０３％）和９６．０９％（ＲＳＤ＝２．０１     

薄层扫描法测定扑尔伪麻片中有效成分的含量

以硅胶ＧＦ２５４为吸附剂，氯仿－甲醇－氨水（９∶１∶０１）为展开剂，采用双波长薄层扫描法同时测定了扑尔伪麻片中的扑尔敏和盐酸伪麻黄碱的含量。扑尔敏在紫外灯下检视，λＳ＝２５６ｎｍ，λＲ＝２８５ｎｍ；盐酸伪麻黄碱采用茚三酮显色，λＳ＝５１０ｎｍ，λＲ＝７００ｎｍ，两组分的平均回收率分别为９９３５％和９９０７％，ＲＳＤ分别为１０８％和０８１％。     

轻燕减肥胶囊质量标准研究

目的：制定轻燕减肥胶囊的质量标准。方法：采用薄层色谱法对防己、大黄、陈皮进行定性鉴别;并建立粉防己碱含量的检测方法,采用薄层扫描法测定。层析条件：硅胶Ｇ薄层板,氯仿－丙酮－甲醇（６：１：１）为展开剂,稀碘化铋钾为显色剂,双波长反射法锯齿扫描,λＳ＝４．９５ｎｍ,λＲ＝６００ｎｍ,ＳＸ＝３。结果：粉防己碱的量在０．５～３．２μｇ范围内具有良好的线性关系,ｒ＝０．９９９９,平均回收率为９５．１％,ＲＳ     

薄层扫描法测定三灵通脉口服液中人参皂甙Rb1含量

采用大孔吸附树脂－薄层扫描法测定三灵通脉口服液中人参皂甙Ｒｂ＿１含量。展开剂：氯仿－甲醇－水（６５：３５：１０）。显色剂：１０％乙醇硫酸溶液。扫描条件：λ＿ｓ＝５３５，λ＿Ｒ＝６８０，ＳＸ＝５。线性范围：０～１０μｇ，γ＝０．９９９４。平均回收率９９．１±３．０％。结果表明，本方法回收率好、分离率高，可作为本制剂的含量测定方法。     

薄层扫描法测定洁阴洗液中苦参碱的含量

目的：采用双波长薄层扫法测定洁阴洗液中苦参碱的含量。方法：用反射式锯齿扫描法测定,测定波长λｓ＝５２０ｎｍ,参比波长λＲ＝ｎｍ;展开剂为：氯仿－甲醇－氨水（３０：１：０．５）,显色剂为稀碘化铋钾试液。结果：点样量在０．６１３￣３．０６４μｇ范围内呈良好的线性关系,平均回收率为９９．５４％,ＲＳＤ为１．３５％（ｎ＝６）。结论：该法分离效果好,简便,快速,结果准确,适用于该制剂的质量控制。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.