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1.
Ekblad T Tran T Orlova A Widström C Feldwisch J Abrahmsén L Wennborg A Karlström AE Tolmachev V 《European journal of nuclear medicine and molecular imaging》2008,35(12):2245-2255
Purpose Affibody molecules are low molecular weight proteins (7 kDa), which can be selected to bind to tumour-associated target proteins
with subnanomolar affinity. Because of rapid tumour localisation and clearance from nonspecific compartments, Affibody molecules
are promising tracers for molecular imaging. Earlier, 99mTc-labelled Affibody molecules demonstrated specific targeting of tumour xenografts. However, the biodistribution was suboptimal
either because of hepatobiliary excretion or high renal uptake of the radioactivity. The goal of this study was to optimise
the biodistribution of Affibody molecules by chelator engineering.
Materials and methods Anti-HER2 ZHER2:342 Affibody molecules, carrying the mercaptoacetyl-glutamyl-seryl-glutamyl (maESE), mercaptoacetyl-glutamyl-glutamyl-seryl (maEES)
and mercaptoacetyl-seryl-glutamyl-glutamyl (maSEE) chelators, were prepared by peptide synthesis and labelled with 99mTc. The tumour-targeting capacity of these conjugates was compared with each other and with the best previously available
conjugate, 99mTc-maEEE-ZHER2:342, in nude mice bearing SKOV-3 xenografts. The tumour-targeting capacity of the most promising conjugate, 99mTc-maESE-ZHER2:342, was compared with radioiodinated ZHER2:342.
Results All novel conjugates demonstrated successful tumour targeting and a low degree of hepatobiliary excretion. The renal uptakes
of serine-containing conjugates, 33 ± 5, 68 ± 21 and 71 ± 10%IA/g, for99mTc-maESE-ZHER2:342, 99mTc-maEES-ZHER2:342 and 99mTc-maSEE-ZHER2:342, respectively, were significantly reduced in comparison with 99mTc-maEEE-ZHER2:342 (102 ± 13%IA/g). For 99mTc-maESE-ZHER2:342, a tumour uptake of 9.6 ± 1.8%IA/g and a tumour-to-blood ratio of 58 ± 6 were reached at 4 h p.i.
Conclusions A combination of serine and glutamic acid residues in the chelator sequence confers increased renal excretion and relatively
low renal uptake of 99mTc-labelled Affibody molecules. In combination with preserved targeting capacity, this improved imaging of targets in abdominal
area. 相似文献
2.
Gomes CM Welling M Que I Henriquez NV van der Pluijm G Romeo S Abrunhosa AJ Botelho MF Hogendoorn PC Pauwels EK Cleton-Jansen AM 《European journal of nuclear medicine and molecular imaging》2007,34(11):1793-1803
Purpose The purpose of this work was the development of an orthotopic model of osteosarcoma based on luciferase-expressing tumour
cells for the in vivo imaging of multidrug resistance (MDR) with 99mTc-sestamibi.
Methods Doxorubicin-sensitive (143B-luc+) and resistant (MNNG/HOS-luc+) osteosarcoma cell lines expressing different levels of P-glycoprotein and carrying a luciferase reporter gene were inoculated
into the tibia of nude mice. Local tumour growth was monitored weekly by bioluminescence imaging and X-ray. After tumour growth,
a 99mTc-sestamibi dynamic study was performed. A subset of animals was pre-treated with an MDR inhibitor (PSC833). Images were
analysed for calculation of 99mTc-sestamibi washout half-life (t
1/2), percentage washout rate (%WR) and tumour/non-tumour (T/NT) ratio.
Results A progressively increasing bioluminescent signal was detected in the proximal tibia after 2 weeks. The t
1/2 of 99mTc-sestamibi was significantly shorter (p < 0.05) in drug-resistant MNNG/HOS-luc+ tumours (t
1/2 = 87.3 ± 15.7 min) than in drug-sensitive 143B-luc+ tumours (t
1/2 = 161.0 ± 47.4 min) and decreased significantly with PSC833 (t
1/2 = 173.0 ± 24.5 min, p < 0.05). No significant effects of PSC833 were observed in 143B-luc+ tumours. The T/NT ratio was significantly lower (p < 0.05) in MNNG/HOS-luc+ tumours than in 143B-luc+ tumours at early (1.55 ± 0.22 vs 2.14 ± 0.36) and delayed times (1.12 ± 0.11 vs 1.62 ± 0.33). PSC833 had no significant effects
on the T/NT ratios of either tumour.
Conclusion The orthotopic injection of tumour cells provides an animal model suitable for functional imaging of MDR. In vivo bioluminescence
imaging allows the non-invasive monitoring of tumour growth. The kinetic analysis of 99mTc-sestamibi washout provides information on the functional activity of MDR related to P-glycoprotein expression and its pharmacological
inhibition in osteosarcoma. 相似文献
3.
Lin KJ Wu CC Pan YH Chen FH Fu SY Chiang CS Hong JH Lo JM 《Annals of nuclear medicine》2012,26(3):272-280
Objective
A recombinant annexin A5 with the N-terminal extension of six histidine residues was labeled with 99mTc(I)-tricarbonyl ion to produce the 99mTc-labeled annexin A5, referred to 99mTc(I)-his6-annexin A5. We have explored the agent as an effective imaging probe for in vivo detecting the apoptosis of internal tissue subjected with high radiation doses in a γ-irradiated mouse model. 相似文献4.
Zhao Y Kuge Y Zhao S Morita K Inubushi M Strauss HW Blankenberg FG Tamaki N 《European journal of nuclear medicine and molecular imaging》2007,34(11):1747-1755
Purpose
99mTc-annexin A5, a marker of ongoing apoptosis, and 18F-FDG, a marker of the increased metabolism of inflammatory cells, are supposed to be useful in the detection of metabolically
active atheroma. This study reports a comparison of the intralesional distribution of these tracers in relation to lesion
development in ApoE−/− mice.
Methods Male ApoE−/− mice (n = 12–14/group) were maintained on a Western-type diet after the age of 5 weeks. At 25 weeks, 99mTc-annexin A5 or 18F-FDG was injected and the aortas were harvested for autoradiography (ARG) and Oil Red O staining. Regional radioactivity
accumulation was compared in relation to the Oil Red O staining score (ranging from 0 to 3, a semiquantitative parameter for
evaluating lesion development).
Results Both 99mTc-annexin A5 and 18F-FDG showed preferential uptake into atherosclerotic lesions, with higher uptake levels for 18F-FDG (mean, 56.07 %ID×kg/m2) than for 99mTc-annexin A5 (mean, 10.38 %ID×kg/m2). The regional uptake levels of each tracer correlated with the Oil Red O staining score (r = 0.65, p < 0.05 for 99mTc-annexin A5; r = 0.56, p < 0.05 for 18F-FDG). The uptake ratios of advanced lesions (score >0.5) to early lesions (score <0.5) were significantly higher for 99mTc-annexin A5 than for 18F-FDG (f = 4.73, p = 0.03).
Conclusion Both 99mTc-annexin A5 and 18F-FDG accumulate in atherosclerotic lesions and correlate with the severity of each lesion. The higher absolute uptake levels
of 18F-FDG may be advantageous for lesion detection, whereas the preferential uptake of 99mTc-annexin A5 in advanced lesions may be a useful indicator of late-stage lesions or vulnerable plaque transformation. 相似文献
5.
M. Pissarek J. Meyer-Kirchrath T. Hohlfeld S. Vollmar A. M. Oros-Peusquens U. Flögel C. Jacoby U. Krügel N. Schramm 《European journal of nuclear medicine and molecular imaging》2009,36(9):1495-1509
Purpose The study serves to optimise conditions for multi-pinhole SPECT small animal imaging of 123I- and 99mTc-labelled radiopharmaceuticals with different distributions in murine heart and brain and to investigate detection and dose
range thresholds for verification of differences in tracer uptake.
Methods A Triad 88/Trionix system with three 6-pinhole collimators was used for investigation of dose requirements for imaging of
the dopamine D2 receptor ligand [123I]IBZM and the cerebral perfusion tracer [99mTc]HMPAO (1.2–0.4 MBq/g body weight) in healthy mice. The fatty acid [123I]IPPA (0.94 ± 0.05 MBq/g body weight) and the perfusion tracer [99mTc]sestamibi (3.8 ± 0.45 MBq/g body weight) were applied to cardiomyopathic mice overexpressing the prostaglandin EP3 receptor.
Results In vivo imaging and in vitro data revealed 45 kBq total cerebral uptake and 201 kBq cardiac uptake as thresholds for visualisation
of striatal [123I]IBZM and of cardiac [99mTc]sestamibi using 100 and 150 s acquisition time, respectively. Alterations of maximal cerebral uptake of [123I]IBZM by >20% (116 kBq) were verified with the prerequisite of 50% striatal of total uptake. The labelling with [99mTc]sestamibi revealed a 30% lower uptake in cardiomyopathic hearts compared to wild types. [123I]IPPA uptake could be visualised at activity doses of 0.8 MBq/g body weight.
Conclusion Multi-pinhole SPECT enables detection of alterations of the cerebral uptake of 123I- and 99mTc-labelled tracers in an appropriate dose range in murine models targeting physiological processes in brain and heart. The
thresholds of detection for differences in the tracer uptake determined under the conditions of our experiments well reflect
distinctions in molar activity and uptake characteristics of the tracers.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
6.
Yared Tekabe Qing Li Joane Luma Drew Weisenberger Marija Sedlar Evis Harja Jagat Narula Lynne L. Johnson 《Journal of nuclear cardiology》2010,17(6):1073-1081
Objectives
To compare the ability of 99mTc-labeled broad-based matrix metalloproteinase inhibitor (RP805) (MPI) and 99mTc-annexin V to identify more advanced atherosclerotic disease in apolipoprotein E-null (apoE−/−) mice. 相似文献7.
Liu Z Okada DR Johnson G Hocherman SD Beju D Okada RD 《European journal of nuclear medicine and molecular imaging》2008,35(3):570-578
Introduction
99mTc-sestamibi has been proposed as a viability imaging agent. The purposes of this study were: (1) to determine the relationship
between myocardial viability and 99mTc-sestamibi kinetics using perfused rat heart models across a full spectrum of viability, (2) to do so under conditions where
myocardial flow was controlled and held constant, and (3) to do so using multiple quantitative methods to assess myocardial
viability.
Methods Twenty-three isolated rat hearts were perfused retrogradely with a modified Krebs-Henseleit (KH) solution. Four groups were
studied: controls (C, n = 6), stunned (S, n = 6), ischemic-reperfused (IR, n = 6), and calcium injured (CAL, n = 5). Following a 20-min baseline and subsequent treatment phase, 99mTc-sestamibi was infused over 60 min (uptake) followed by 60 min clearance. Treatment phases consisted of 20 min no flow for
S, 60 min no flow followed by 60 min reflow for IR, and 10 min infusion of KH solution without calcium followed by 20 min
infusion of KH solution with 2 times normal calcium for CAL hearts. Creatine kinase (CK) assay, triphenyltetrazolium chloride
(TTC) staining, and transmission electron microscopic (TEM) analysis were used to determine tissue viability.
Results Myocardial peak 99mTc-sestamibi uptake (%id) was significantly decreased in IR (4.11 ± 0.22 SEM; p < 0.05) and CAL (1.07 ± 0.13; p < 0.05), but not in S (4.88 ± 0.17) as compared with C (5.99 ± 0.50). One hour fractional retention was 79.3 ± 1.9% for C,
80.3 ± 1.3% for S (p = n.s.), 79.1 ± 1.8% for IR (p = n.s.), and 14.9 ± 4.3% for CAL (p < 0.05 compared to all other groups). 99mTc-sestamibi absolute retention (%id) 1 h after the end of tracer administration was significantly decreased in IR (3.26 ± 0.23)
and CAL (0.15 ± 0.02) as compared with both S (3.92 ± 0.16) and C (4.52 ± 0.32) (p < 0.05). CK increased significantly from baseline in the IR and CAL hearts. TTC determined percent viability was 100 ± 0%
for C, 98.3 ± 1.1% for S, 82.8 ± 2.6% for IR, and 0.0 ± 0% for CAL. TEM analysis supported these findings. End tracer activity
was significantly correlated with TTC determined percentage viable myocardium (r = 0.93, p < 0.05) and CK leak (r = −0.90, p < 0.05).
Conclusion
99mTc-sestamibi myocardial activity is significantly reduced in areas of nonviability after 1 h of tracer uptake and 1 h of tracer
clearance. There is a linear correlation between myocardial viability, as determined by three independent methods, and tracer
activity.
This work was supported by the American Heart Association, the Anne and Henry Zarrow Foundation, and the William K. Warren
Medical Research Foundation. 相似文献
8.
Sotgia B Sciagrà R Parodi G Kastrati A Antoniucci D Schömig A Pupi A 《European journal of nuclear medicine and molecular imaging》2008,35(5):906-911
Purpose We hypothesized that, because of persistent stunning, the extent of post-treatment functional abnormalities detected using
gated single-photon emission computed tomography (SPECT) could be representative of the initial risk area in acute myocardial
infarction (AMI) treated by reperfusion therapy.
Materials and methods In 48 AMI patients, we acquired two 99mTc-sestamibi gated SPECT studies (at admission with tracer injection before treatment and at discharge 5 to 10 days later).
We assessed the myocardial salvage defined by the admission minus predischarge summed rest score, and we compared it with
the value obtained by subtracting the extent of perfusion defect from the extent of wall motion or wall thickening abnormalities
in predischarge gated SPECT. Myocardial salvage was expressed as salvage index (salvaged myocardium divided by initial risk
area).
Results There was a good correlation between summed rest score salvage index and wall motion (Spearman’s ρ = 0.754, p < 0.0001) or wall thickening salvage index (Spearman’s ρ = 0.798, p < 0.0001). The wall thickening salvage index was able to classify correctly the patients that had a summed rest score salvage
index ≥ 0.10 with 73% sensitivity, 88% specificity, and 83% accuracy. The wall motion salvage index was highly sensitive (91%)
but poorly specific (13%, p < 0.002 vs wall thickening salvage index) and less accurate (69%, p < 0.05 vs wall thickening salvage index).
Conclusions
99mTc-sestamibi gated SPECT allows assessing myocardial salvage using only post-treatment data. The salvage index derived using
wall thickening as surrogate of admission perfusion defect correlates well with the salvage index measured by comparing pre-
and post-treatment perfusion defects. 相似文献
9.
Ma Q Ji B Jia B Gao S Ji T Wang X Han Z Zhao G 《European journal of nuclear medicine and molecular imaging》2011,38(12):2145-2152
Purpose
Targeting of integrin ανβ3 with molecular imaging agents offers great potential in early detection and monitoring of tumour angiogenesis. Recently, an RGD (Arg-Gly-Asp) tracer, 99mTc-3P4-RGD2, with high affinity to integrin ανβ3 and in vivo tumour uptake was developed. In this study, we evaluate the feasibility of this novel radiotracer in the noninvasive differentiation of solitary pulmonary nodules (SPNs). 相似文献10.
Cremonesi M Ferrari M Bartolomei M Orsi F Bonomo G Aricò D Mallia A De Cicco C Pedroli G Paganelli G 《European journal of nuclear medicine and molecular imaging》2008,35(11):2088-2096
Introduction Radioembolisation with 90Y-microspheres is a new locoregional treatment of hepatic lesions, usually applied as single cycle. Multi-cycle treatments
might be considered as a strategy to improve the risk-benefit balance. With the aim to derive suitable information for patient
tailored therapy, available patients’ dosimetric data were reviewed according to the linear–quadratic model and converted
into biological effective dose (BED) values. Single vs. multi-cycle approaches were compared through radiobiological perspective.
Materials and methods Twenty patients with metastatic lesions underwent radioembolisation. The 90Y-administered activity (AA) was established in order to respect a precautionary limit dose (40 Gy) for the non-tumoral liver
(NTL). BED was calculated setting α/β = 2.5 Gy (NTL), 10 Gy (tumours); T
1/2,eff = T
1/2,phys = 64.2 h; T
1/2,rep = 2.5 h (NTL), 1.5 h (tumours). The BED to NTL was considered as a constraint for multi-cycle approach. The AA for two cycles
and the percent variations of AA, tumour dose, BED were estimated.
Results In one-cycle, for a prescribed BED to NTL of 64 Gy (NTL dose = 40 Gy), AA was 1.7 (0.9–3.2) GBq, tumour dose was 130 (65–235)
Gy, and tumour BED was 170 (75–360) Gy. Considering two cycles, ∼15% increase was found for AA and dose to NTL, with unvaried
BED for NTL. Tumour dose increase was 20 (10–35) Gy; tumour BED increase was 10 (3–11) Gy. In different protocols allowing
80 Gy to NTL, the BED sparing estimated was ∼50 Gy (two cycles) and 65 Gy (three cycles).
Conclusions From a radiobiological perspective, multi-cycle treatments would allow administering higher activities with increased tumour
irradiation and preserved radiation effects on NTL. Trials comparing single vs. multiple cycles are suggested. 相似文献
11.
Choong Wook Lee Joon Beom Seo Jae-Woo Song Mi-Young Kim Ha Young Lee Yang Shin Park Eun Jin Chae Yu Mi Jang Namkug Kim Bernard Krauss 《European radiology》2011,21(1):54-62
Purpose
To evaluate the sensitivity of computer-aided detection(CAD) and dual-energy software(‘Lung PBV’, ‘Lung Vessels’) for detecting peripheral pulmonary embolism(PE). 相似文献12.
Hirvonen J Aalto S Hagelberg N Maksimow A Ingman K Oikonen V Virkkala J Någren K Scheinin H 《European journal of nuclear medicine and molecular imaging》2009,36(2):275-286
Purpose [11C]Carfentanil has been widely used in positron emission tomography (PET) studies for measuring μ-opioid receptor binding in
humans, but the reproducibility of the binding parameter estimates is unknown.
Materials and methods Eight healthy volunteers were scanned twice during the same day with [11C]carfentanil PET, and binding to receptors was assessed with both reference tissue and arterial plasma input-based models
using region of interest (ROI) and voxel-based quantification.
Results The two-tissue compartmental model distribution volume (VT) was highly reproducible as indicated by low variability (VAR < 6%) and high intraclass correlation coefficients (ICC > 0.93).
BPND (BP relative to the nondisplaceable tissue compartment) was also highly reproducible (VAR < 10%, ICC > 0.90) both at ROI-
and voxel-level, and reference tissue-based models provided stable estimates after 40 min.
Conclusions The reproducibility of [11C]carfentanil binding parameter estimates is excellent with outcome measures based on both arterial plasma and reference tissue
input, and a scanning time of 40 min appears sufficient. 相似文献
13.
Nakajima K Tamaki N Kuwabara Y Kawano M Matsunari I Taki J Nishimura S Yamashina A Ishida Y Tomoike H 《European journal of nuclear medicine and molecular imaging》2008,35(11):2038-2048
Backgrounds Prediction of left ventricular functional recovery is important after myocardial infarction. The impact of quantitative perfusion
and motion analyses with gated single-photon emission computed tomography (SPECT) on predictive ability has not been clearly
defined in multi-center studies.
Methods A total of 252 patients with recent myocardial infarction (n = 74) and old myocardial infarction (n = 175) were registered from 25 institutions. All patients underwent resting gated SPECT using 99mTc-hexakis-2-methoxy-isobutyl isonitrile (MIBI) and repeated the study after revascularization after an average follow-up
period of 132 ± 81 days. Visual and quantitative assessment of perfusion and wall motion were performed in 5,040 segments.
Results Non-gated segmental percent uptake and end-systolic (ES) percent uptake were good predictors of wall motion recovery and significantly
differed between improved and non-improved groups (66 ± 17% and 55 ± 18%, p < 0.0001 for non-gated; 64 ± 16% and 51 ± 17% for ES percent uptake, p < 0.0001). The area under the curve of receiver operating characteristics curve for non-gated percent uptake, ES percent
uptake, end-diastolic percent uptake and visual perfusion defect score was 0.70, 0.71, 0.61, and 0.56, respectively. Sensitivity
and specificity of percent uptake were 68% and 64% for non-gated map and 80% and 52% for ES percent uptake map. An optimal
threshold for predicting segmental improvement was 63% for non-gated and 52% for ES percent uptake values.
Conclusion Segmental 99mTc-MIBI uptake provided a useful predictor of wall motion improvement. Application of quantitative approach with non-gated
and ES percent uptake enhanced predictive accuracy over visual analysis particularly in a multi-center study. 相似文献
14.
Jiyun Shi Lijun Wang Young-Seung Kim Shizhen Zhai Bing Jia Fan Wang Shuang Liu 《European journal of nuclear medicine and molecular imaging》2009,36(11):1874-1884
Purpose
This report presents the synthesis of a cyclic RGD dimer conjugate, MAG2-G3-E[G3-c(RGDfK)]2 (MAG2-3G3-dimer, G3 = Gly-Gly-Gly, MAG2 = S-benzoyl mercaptoacetylglycylglycyl), and evaluation of its 99mTc complex, 99mTcO(MAG2-3G3-dimer), as a new radiotracer for imaging the tumor integrin αvβ3 expression. 相似文献15.
Ronald C. Rohe Stephen R. Thomas Michael G. Stabin Edward A. Deutsch Myron C. Gerson Dwight D. Cummings Harry R. Maxon 《Journal of nuclear cardiology》1995,2(5):395-404
Background
Trans (N,N′-ethylene bis(acetylacetoneimine)) bis(tris(3-methoxy-1-propyl) phosphine) 99mTc(III) (99mTc-Q3) has been developed for myocardial perfusion imaging. Biokinetic studies and dosimetry analysis have been completed for six healthy volunteers. 相似文献16.
Akutsu Y Kaneko K Kodama Y Li HL Nishimura H Hamazaki Y Suyama J Shinozuka A Gokan T Kobayashi Y 《European journal of nuclear medicine and molecular imaging》2009,36(2):230-236
Purpose Microcirculatory failure after reperfusion is clinically indicated to cause reperfusion injury whereas excessive intracellular
calcium ion overload is experimentally proved as a key mechanism of reperfusion injury. We hypothesized that technetium-99m
(99mTc) pyrophosphate (Tc-PYP) uptake in injured but viable infarct-related myocardium with preserved myocardial perfusion after
reperfusion estimated by thallium-201 (201Tl) uptake would be associated with final functional recovery.
Methods Dual-isotope Tc-PYP/201Tl single-photon emission computed tomography (SPECT) was performed 2 days after successful reperfusion therapy in patients
with first acute myocardial infarction, and 50 patients (63 ± 13 years old, female 22%) with preserved 201Tl uptakes of ≥50% in reperfused myocardium was followed for 1 month. Tc-PYP uptake was assessed as the heart-to-sternum (H/S)
ratio. Two-dimensional echocardiography was also performed 2 days and 1 month after reperfusion to evaluate functional recovery.
Results High Tc-PYP uptake, defined as the H/S ratio ≥0.81, was predictive of chronic phase no functional recovery (73.7% in 14 of
19 patients with high uptake vs 16.1% in five of 31 patients without those, p < 0.0001). After adjustment for potential confounding variables, including electrocardiographic persistent ST segment elevation
at 1 h after reperfusion, high Tc-PYP uptake remained independently predictive of no functional recovery with odds ratio of
8.7 (95% confidential interval = 2 to 38.7; p = 0.005).
Conclusion High Tc-PYP uptake in reperfused but viable infarct-related myocardium was a powerful predictor of no functional recovery,
which may reflect excessive intracellular calcium ion overload caused by reperfusion injury. Tc-PYP/201Tl dual-isotope SPECT imaging can provide prognostic information after reperfusion. 相似文献
17.
Iida H Eberl S Kim KM Tamura Y Ono Y Nakazawa M Sohlberg A Zeniya T Hayashi T Watabe H 《European journal of nuclear medicine and molecular imaging》2008,35(5):896-905
Purpose
201Tl has been extensively used for myocardial perfusion and viability assessment. Unlike 99mTc-labelled agents, such as 99mTc-sestamibi and 99mTc-tetrofosmine, the regional concentration of 201Tl varies with time. This study is intended to validate a kinetic modelling approach for in vivo quantitative estimation of
regional myocardial blood flow (MBF) and volume of distribution of 201Tl using dynamic SPECT.
Methods Dynamic SPECT was carried out on 20 normal canines after the intravenous administration of 201Tl using a commercial SPECT system. Seven animals were studied at rest, nine during adenosine infusion, and four after beta-blocker
administration. Quantitative images were reconstructed with a previously validated technique, employing OS-EM with attenuation-correction,
and transmission-dependent convolution subtraction scatter correction. Measured regional time–activity curves in myocardial
segments were fitted to two- and three-compartment models. Regional MBF was defined as the influx rate constant (K
1) with corrections for the partial volume effect, haematocrit and limited first-pass extraction fraction, and was compared
with that determined from radio-labelled microspheres experiments.
Results Regional time–activity curves responded well to pharmacological stress. Quantitative MBF values were higher with adenosine
and decreased after beta-blocker compared to a resting condition. MBFs obtained with SPECT (MBFSPECT) correlated well with the MBF values obtained by the radio-labelled microspheres (MBFMS) (MBFSPECT = −0.067 + 1.042 × MBFMS, p < 0.001). The three-compartment model provided better fit than the two-compartment model, but the difference in MBF values
between the two methods was small and could be accounted for with a simple linear regression.
Conclusion Absolute quantitation of regional MBF, for a wide physiological flow range, appears to be feasible using 201Tl and dynamic SPECT. 相似文献
18.
Miot-Noirault E Vidal A Pastoureau P Bonafous J Chomel A Sarry L Audin L Madelmont JC Moins N 《European journal of nuclear medicine and molecular imaging》2007,34(8):1280-1290
Purpose This study in the meniscectomised guinea pig aimed to demonstrate that the radiotracer 99mTc-NTP 15-5 would have pathophysiological validity for in vivo osteoarthritis imaging.
Methods The specificity of 99mTc-NTP 15-5 for cartilage was determined in healthy animals (n = 13), by tissue radioactivity counting, joint autoradiography and scintigraphy. 99mTc-NTP 15-5 scintigraphy was performed at 20, 50, 80, 115, 130, 150 and 180 days after medial meniscectomy (n = 10 MNX) or sham operation (n = 5), and scintigraphic ratios (operated/contralateral) were calculated for femoral (F) and tibial (T) areas. F and T ratios
were compared with those of 99mTc-MDP bone scintigraphy. At the study end-point, autoradiographic analysis of joint 99mTc-NTP 15-5 distribution and macroscopic scoring of cartilage integrity were performed.
Results The high and specific accumulation of 99mTc-NTP 15-5 in normal cartilage (about 5.5 ± 1.7 % of injected dose/g of tissue), which permitted joint imaging with high
contrast, was affected by osteoarthritis. In the MNX group, 99mTc-NTP 15-5 accumulation in cartilage within the operated joint, relative to the contralateral joint, was observed to change
in the same animals as pathology progressed. Although F and T ratios were significantly higher in MNX (F = 1.7 ± 0.2; T = 1.6 ± 0.1)
than in shams (F = 1.0 ± 0.1; T = 1.0 ± 0.1) at day 50, they were significantly lower in MNX (F = 0.6 ± 0.1; T = 0.7 ± 0.1)
than in shams (F = 1.0 ± 0.1; T = 0.9 ± 0.1) at day 180. No change in 99mTc-MDP uptake was observed over 6 months. Macroscopic analysis confirmed features of osteoarthritis only in MNX knees.
Conclusion These results in MNX guinea pigs provide additional support for the use of 99mTc-NTP 15-5 for in vivo imaging of osteoarthritis. 相似文献
19.
Ishino S Kuge Y Takai N Tamaki N Strauss HW Blankenberg FG Shiomi M Saji H 《European journal of nuclear medicine and molecular imaging》2007,34(6):889-899
Purpose Apoptosis is commonly observed in advanced atherosclerotic lesions. 99mTc-annexin A5 (99mTc-annexin V) has been proposed as a potential tracer for imaging apoptosis in atherosclerotic plaques. Accordingly, we determined
the usefulness of 99mTc-annexin A5 as an atherosclerosis imaging tracer in a rabbit model (myocardial infarction-prone Watanabe heritable hyperlipidemic
rabbits; WHHLMI rabbits) of spontaneous atherosclerosis.
Methods The WHHLMI and control rabbits were injected intravenously with 99mTc-annexin A5. After in vivo planar imaging, the radioactivity in the aorta was measured. Autoradiography, TUNEL staining,
Azan-Mallory staining and immunohistological studies were performed serially throughout the aorta.
Results
99mTc-Annexin A5 accumulation in the aorta of the WHHLMI rabbits was 5.6-fold higher than in that of control rabbits. Autoradiography
showed heterogeneous multifocal accumulation of 99mTc-annexin A5 in WHHLMI rabbits. 99mTc-Annexin A5 accumulation was highest in the atheromatous lesions (6.2 ± 2.5, %ID × BW/mm2 × 103), followed in decreasing order by neointimal (4.9 ± 1.3), fibroatheromatous (4.5 ± 1.9), and collagen-rich lesions (3.3 ± 1.4).
The regional 99mTc-annexin A5 accumulation was significantly correlated with the TUNEL-positive cell density, macrophage density and “vulnerability
index,” an index of the morphological destabilized characteristics. The in vivo imaging clearly visualized the atherosclerotic
lesions in WHHLMI rabbits.
Conclusion The present study in WHHLMI rabbits showed higher 99mTc-annexin A5 accumulation in grade IV atheroma than in other more stable lesions. 99mTc-Annexin A5 may be useful in identifying atheroma that is at higher risk for rupture and possibly in assessing the response
to anti-atherosclerotic therapy. 相似文献
20.
Masanobu Ibaraki Kaoru Sato Tetsuro Mizuta Keishi Kitamura Shuichi Miura Shigeki Sugawara Yuki Shinohara Toshibumi Kinoshita 《Annals of nuclear medicine》2009,23(7):627-638