脂肪细胞因子与胰岛素抵抗的相关性分析

目的探讨肥胖及糖尿病患者血清脂肪细胞因子即:脂联素、肿瘤坏死因子(TNF)及瘦素水平与发生胰岛素抵抗的关系。方法糖耐量正常者和2型糖尿病患者各60例,其中肥胖受试者60例,体重正常者60例。所有受试者均测其脂联素、TNF-α、瘦素、血糖和血脂的水平以及空腹血清免疫反应性胰岛素(FIRI),并测定血压,腰围臀围比值(WHR)。结果糖尿病组的血清脂联素水平明显低于糖耐量正常组,其值分别为(6·09±3·11)mg/L和(14·12±4·17)mg/L,两者有统计学差异(P<0·001)。血清脂联素水平与BMI、FIRI、WHR和胰岛素抵抗指数(HOMA-IR)均负相关(P<0·01)。血清瘦素水平与BMI、FIRI及HOMA-IR明显正相关(P<0·01)。TNF-α与BMI、WHR、FIRI、HOMA-IR呈显著正相关(P<0·01)。血清脂联素与HDL-C呈正相关,而瘦素和TNF-α与HDL-C呈明显的负相关(P<0·01)。统计学上均具有显著意义。血清脂联素水平与TNF-α及瘦素水平呈明显负相关(分别为r=-0·40,P<0·001;r=-0·40,P<0·001)。结论结果显示,脂肪细胞因子即:脂联素、TNF-α及瘦素水平直接或间接参与正常人和糖尿病患者肥胖及胰岛素抵抗的发生或发展。     

瘦素、脂联素、抵抗素、内肥素与妊娠糖尿病胰岛素抵抗的相关性研究

目的:探讨脂肪因子瘦素、脂联素、抵抗素、内肥素与妊娠糖尿病(GDM)胰岛素抵抗的相关性.方法:实验分为正常育龄妇女组(44例)、正常妊娠妇女组(62例)和妊娠糖尿病患者组(63例),用ELISA方法分别检测3组瘦素(Leptin),脂联素(Adiponectin),抵抗素(Resistin),内肥素(Visfatin)的水平,用葡萄糖氧化酶法检测血糖水平,免疫发光法检测胰岛素水平,用HOMA-IR(稳态模型胰岛素抵抗指数)表示胰岛素抵抗状态,HOMA-IR=空腹血糖×空腹血胰岛素÷22.5,用SPSSll.5统计软件进行统计分析.结果:妊娠糖尿病患者组的瘦素水平明显高于正常妊娠妇女组[(13.50±7.14)ng/ml、(7.78±3.82)ng/ml,P<0.001],正常妊娠妇女组明显高于正常育龄妇女组[(7.78±3.82)ng/ml、(1.73±0.70)ng/ml,P<0.001]差异具有统计学意义;GDM 组的内肥素水平明显低于正常妊娠妇女组[(15.67±5.78)ng/ml、(22.43±5.68)ng/ml,P<0.001],正常妊娠妇女组明显低于正常育龄妇女组[(22.43±5.68)ng/ml、(36.46±13.34)ng/ml,P<0.001],差异具有统计学意义;GDM 组的脂联素水平低于正常妊娠妇女组[(5 491.71±2 986.00)ns/ml、(6 692.34±2 583.51)ng/ml,P=O.029],差异有统计学意义,正常妊娠妇女组明显低于正常育龄妇女组[(6 692.34±2 583.51)ng/ml、(11 076.82±3 694.75)ng/ml,P<0.1301],差异具有统计学意义;GDM组抵抗素水平与正常妊娠妇女组比较差异没有统计学意义.在妊娠糖尿病患者组,HOMA-IR与瘦素(r=0.352,P=0.005)正相关,与内肥素(,=-0.255,P=0.046)呈负相关.结论:妊娠糖尿病患者瘦素水平升高,内肥素和脂联素水平降低,瘦素和内肥素与胰岛素抵抗有关,瘦素和内肥素参与了妊娠糖尿病胰岛素抵抗的发生发展;脂联素和抵抗素与胰岛素抵抗不具有相关性,脂联素和抵抗素可能与妊娠糖尿病胰岛素抵抗的发生无关.     

瘦素、脂联素与TNF-α在子痫前期胎盘中的表达及相关性研究

目的检测子痫前期胎盘中瘦素、脂联素及TNF-α的表达并探讨TNF-α与另两个因子的相关性。方法采用免疫组化方法检测55例子痫前期患者及21例正常对照组胎盘瘦素、脂联素及TNF-α的表达水平。结果重度子痫前期组胎盘瘦素表达水平显著高于正常组(P<0.01),轻度组与正常组无统计学差异(P>0.05);重度组胎盘脂联素表达水平显著低于正常组(P<0.01),轻度组与正常组无统计学差异(P>0.05);子痫前期胎盘TNF-α表达水平显著高于正常组(P<0.05及P<0.01);重度组瘦素与TNF-α成正相关(r=0.536),而正常组与病例组中脂联素与TNF-α均无相关性(r=0.416r、=0.398及r=0.254)。结论脂联素、瘦素及TNF-α参与子痫前期的发病,且TNF-α可调节瘦素的水平,但对脂联素的调节不明显。     

瘦素、脂联素与TNF-α在子痫前期患者血清中的表达及相关性研究

目的检测子痫前期患者血清瘦素、脂联素及TNF-α的表达并探讨TNF-α与瘦素、脂联素的相关性。方法采用酶联免疫吸附法(ELISA)检测44例子痫前期患者及24例正常孕妇血清中瘦素、脂联素及TNF-α的表达水平。结果(1)轻、重度子痫前期患者血清瘦素水平分别为4308.60±378.83pg/ml、4476.39±115.18pg/ml,明显高于对照组4041.97±455.39pg/ml,差异有统计学意义(P<0.05,P<0.01);(2)轻度、重度子痫前期患者血清TNF-α水平分别为73.33±57.91pg/ml及538.50±551.25pg/ml,明显高于对照组62.89±82.85pg/ml,而血清脂联素水平分别为8.06±5.06pg/ml、5.28±1.47pg/ml,明显低于对照组12.73±2.90pg/ml,重度组与对照组之间的差异有统计学意义(P<0.05);(3)重度组瘦素与TNF-α成正相关(r=0.478),脂联素与TNF-α成负相关(r=-0.536),轻度组及对照组中TNF-α与瘦素、脂联素均无相关性。结论瘦素、脂联素及TNF-α参与子痫前期的发病,且TNF-α可以调节瘦素及脂联素的水平。     

TLR4 基因敲除对小鼠内脏脂肪组织免疫细胞及脂肪因子的影响

目的:探讨TLR4 基因敲除对小鼠免疫细胞及脂肪因子的影响。方法:取20 周龄的雄性野生型C57BL/6 小鼠和TLR4-/ -小鼠的脾脏和附睾脂肪组织,分离细胞,用流式检测F4/80、CD11b、CD11c、CD3、CD4、CD8 分子的表达;qPCR 检测附睾脂肪组织内IL-6、HMGB1、TNF-α、脂联素和抵抗素的表达。结果:与野生型C57BL/6 小鼠相比,TLR4-/ - 小鼠脾脏和附睾脂肪组织中M1 型(F4/80+ CD11b+ CD11c+ )巨噬细胞比例上升(P<0.05),M2 型(F4/80+ CD11b+ CD11c- )巨噬细胞比例下降(P<0.05),这种趋势在附睾脂肪组织中表现更为显著。同时发现附睾脂肪组织中CD4+ T 细胞比例下降(P<0.05),CD8+ T细胞比例上升(P<0.05);IL-6、HMGB1、抵抗素表达升高(P<0.05);TNF-α和脂联素表达降低(P<0.05)。结论:TLR4 基因敲除可导致内脏脂肪组织脂肪因子和免疫细胞的紊乱。     

脂肪因子与代谢综合征

脂肪因子是一系列由脂肪组织分泌的生物活性物质,包括瘦素、抵抗素、脂联素、内肥素、肿瘤坏死因子、纤溶酶原激活物抑制因子和白介素-6等。脂肪因子的紊乱参与肥胖、糖尿病及动脉粥样硬化等多种代谢性疾病的发病过程。本文将着重讨论脂肪因子与代谢综合征的关联。     

男性双相情感障碍患者外周血相关基因表达与血清细胞因子水平的相关性研究

目的 研究男性双相情感障碍(bipolar disorder,BD)患者外周血谷氨酸受体亚单位-1(GluD1)、突触结合蛋白7(Syt7)、M2型瞬时受体电位(TRPM2)以及甲壳质酶蛋白40(YKL-40)基因表达变化,并探讨与血清炎性细胞因子水平的相关性.方法 选择我院2017年5月至2019年5月收治的86例男性BD患者为观察组;同时期来我院健康男性体检者40例作为对照组.qRT-PCR法检测对照组和观察组患者外周血GluD1、YKL-40、Syt7、TRPM2基因的表达水平;酶联免疫吸附法检测血清炎性细胞因子IL-1、IL-4、IL-6、IL-10以及TNF-α的水平,并对相关基因表达水平与炎性细胞因子水平的关系进行分析.结果 与对照组比较,观察组基因GluD1和Syt7的表达水平明显降低,而TRPM2和YKL-40的表达水平明显升高(P<0.05),血清炎性细胞因子IL-1、IL-4以及TNF-α水平显著升高(P<0.05),而IL-6及IL-10水平差异无统计学意义(P>0.05).相关性分析结果显示,GluD1基因水平与IL-1和TNF-α水平呈负相关(r=-0.251,-0.200;均P<0.05),Syt7基因水平与IL-1水平呈负相关(r=-0.186,P<0.05),TRPM2基因水平与IL-6水平呈正相关(r=0.190,P<0.05),YKL-40基因水平与IL-1和IL-6水平呈正相关(r=0.187,0.220;均P<0.05).结论 GluD1、Syt7、TRPM2以及YKL-40基因在男性BD患者外周血中差异表达,且与不同的血清炎性细胞因子水平相关.     

老年糖尿病酮症酸中毒患者IL-6和氧化应激的变化

目的: 探讨老年糖尿病酮症酸中毒(DKA)患者白细胞介素6(IL-6)和氧化应激的变化.方法:检测老年糖尿病酮症酸中毒患者治疗前后血IL6、 8异前列腺素F-2α(8-isoprostaglandinF2α,8isoPGF2α)水平、超氧化物歧化酶(SOD）活性、总抗氧化能力(TAC)和丙二醛(MDA）含量.结果:在DKA患者SOD、 TAC显著低于对照组(P<0.05), 而IL6、 MDA和8isoPGF2α显著高于对照组(P<0.05); DKA患者治疗后的SOD和TAC显著高于治疗前患者(P<0.05), 而IL-6、 MDA和8isoPGF2α显著低于治疗前患者(P<0.05).DKA患者治疗前IL6与8isoPGF2α呈显著性正相关(r=0.33, P<0.05）, 治疗后IL-6与SOD呈显著性负相关(r=-0.36, P<0.05）.DKA患者治疗前后IL-6与MDA均呈显著性正相关(r=0.38, 0.41, P<0.05）.结论: DKA患者血清IL-6水平明显升高, 且与氧化应激有关.     

瘦素和脂联素在儿童肥胖相关性高血压发病中的作用

目的探讨瘦素和脂联素在儿童肥胖相关性高血压发病中的作用。方法基于北京市儿童青少年代谢综合征研究项目的现况调查结果,非随机选择3502名6-18岁学龄儿童（其中男1784名,女1718名）为研究对象,按照超重（包括肥胖）和高血压状态将研究对象分为4组,正常体重正常血压组（对照组,1497名）、正常体重高血压组（HBP组,125名）、超重但血压正常组（OB组,1349名）和超重合并高血压组（OB＋HBP组,531名）。通过比较4组人群血清瘦素和脂联素水平,以及瘦素和脂联素与血压之间的相关回归分析,探讨其与肥胖和血压之间的关系。结果超重肥胖人群BMI、血压、胰岛素和瘦素水平显著升高,脂联素水平降低。HBP组与对照组BMI、瘦素、脂联素水平差异无统计学意义。OB组和OB＋HBP组与对照组比较,BMI、SBP、DBP、胰岛素和瘦素水平升高,脂联素水平降低,与HBP组比较仍可见BMI、胰岛素和瘦素水平升高,脂联素水平降低。与OB组比较,OB＋HBP组BMI和胰岛素水平及男性的瘦素水平明显升高。血压与年龄、BMI、胰岛素、瘦素均呈显著正相关（r=0.260-0.643,P〈0.01）,与脂联素呈显著负相关（r=-0.171--0.332,P〈0.01）。但在调整胰岛素或BMI后,瘦素、脂联素与血压的相关性减弱或消失。结论超重人群血压、胰岛素及瘦素水平均高于对照人群,脂联素水平低于对照人群。瘦素、脂联素可能通过肥胖或胰岛素抵抗与血压相关。     

子宫内膜异位症与免疫炎症、氧化应激及血管生成的相关性研究

目的 探讨子宫内膜异位症(EM)与免疫炎症、氧化应激及血管生成的相关性.方法 选择90例EM患者作为观察组,另选60名同期孕前健康检查合格的育龄期女性作为对照组,采用酶联免疫吸附法(ELISA法)测定对照组女性及观察组患者手术前后血清中白细胞介素6(IL-6)、IL-8、肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)、谷胱甘肽过氧化物酶(GSH-Px)、8-异前列腺素F2α(8-iso-PGF2α)、血管生成素-2(Ang-2)及血管内皮生长因子(VEGF)水平.结果 观察组患者术前血清IL-6、IL-8、TNF-α、8-iso-PGF2α、Ang-2及VEGF水平均明显高于对照组(P＜0.05),血清IFN-γ和GSH-Px水平明显低于对照组(P＜0.05);术后,观察组患者的血清IL-6、IL-8、TNF-α、8-iso-PGF2α、Ang-2及VEGF水平均较术前显著降低(P＜0.05),血清IFN-γ和GSH-Px水平较术前显著升高(P＜0.05).结论 EM的病理过程与免疫炎症、氧化应激和血管生成密切相关,血清IL-6、IL-8、TNF-α、IFN-γ、GSH-Px、8-iso-PGF2α、Ang-2和VEGF水平检测对EM的诊断及其治疗效果评价具有一定的参考价值.     

Correlation between estrogens and serum adipocytokines in premenopausal and postmenopausal women

OBJECTIVE: The purpose of this study was to investigate the association between serum adipocytokines (adiponectin, resistin, leptin, and tumor necrosis factor alpha [TNF-alpha]) and endogenous estrogen (estrone and estradiol) levels in healthy premenopausal and postmenopausal women. DESIGN: This study included 53 healthy premenopausal women, 45 healthy postmenopausal women, and 10 postmenopausal women with the metabolic syndrome who were participating in general health examinations. A secondary analysis was performed on levels of adiponectin, resistin, leptin, TNF-alpha, estrone (E1), and estradiol (E2). RESULTS: After accounting for body mass index, TNF-alpha was significantly increased (1.5+/-0.1 vs 2.0+/-0.1 pg/mL, P<0.05) in healthy postmenopausal women as compared with healthy premenopausal women, whereas leptin was decreased (5.6+/-1.1 vs 4.0+/-1.1 ng/mL). Estrogen (E1 and E2) was positively correlated with leptin in only healthy premenopausal women, whereas estrogen did not correlate with any adipocytokine in healthy postmenopausal women. In the multiple regression analysis, only leptin significantly contributed to insulin resistance. Combining healthy premenopausal and postmenopausal women, E1 correlated negatively with TNF-alpha (r=-0.23, P<0.05) and positively with leptin (r=0.35, P<0.01) and did not correlate with resistin. E2 correlated negatively with TNF-alpha (r=-0.24, P<0.05) and positively with leptin (r=0.34, P<0.01); it did not correlate with adiponectin or resistin. Leptin might stimulate the increase of plasma gonadotropin-releasing hormone levels, which could result in a positive correlation with estrogen in premenopausal women but not in postmenopausal women. CONCLUSIONS: Estrogen deficiency resulted in increased TNF-alpha levels. Serum leptin levels correlated positively with estrogen levels in premenopausal women. However, the increase in obesity in postmenopausal women increased leptin, which increases insulin resistance.     

Changes in immunologic parameters of humoral immunity and adipocytokines in obese persons are gender dependent

The aim of this study was to investigate several immunologic parameters using of immunonephelometry and adipocytokines by the enzyme immunoassay and their changes in different states of obesity. Obesity is considered to involve a state of chronic low-grade inflammation, with links between adipose cells and the immune system. We found significantly higher complement C3 levels in all obese subjects. Levels of the complement C4 were significantly higher in obese women, but not in men, when compared with the corresponding group of normal weight subjects. The increase in C-reactive protein concentrations was significant in both obese and morbidly obese women, but only in morbidly obese men. No significant differences in tumor necrosis factor-α, interleukin-6, interleukin-10, and soluble intercellular adhesion molecule-1 were found. sE-selectin levels were higher in both overweight and obese women but only in morbidly obese men. We found decreased adiponectin concentrations in obese and morbidly obese women. Concentrations of leptin were significantly higher only in obese men (p < 0.05), whereas in women the increase in leptin levels was significant in overweight, obese, and morbidly obese subjects. In conclusion, our results demonstrate elevated levels of C3, C-reactive protein, sE-selectin, and leptin in obese women and men. In obese women, we also observed increased concentrations of C4 and decreased levels of adiponectin.     

Characterization of fat distribution in Prader–Willi syndrome: Relationships with adipocytokines and influence of growth hormone treatment

Marked anthropometric changes are seen in Prader–Willi syndrome (PWS). Emaciation is observed during infancy, whereas severe obesity is found in older children and adults. Growth hormone (GH) treatment modifies the anthropometric changes in PWS patients. In this study, we examined changes in the body composition of 51 PWS patients (age range, 6–54 years; median, 16.5 years), with a focus on the amount of abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT), VAT/SAT ratio, and serum levels of adipocytokines (adiponectin, leptin, and resistin). The relationships between VAT, SAT, and adipocytokines, and lipid abnormalities and type 2 diabetes in 24 patients with obese PWS were also evaluated. With increasing age, SAT and VAT both increased markedly, but in 18 patients receiving GH treatment, VAT remained low at ≤30 cm². In the GH‐completed patients (n = 19), VAT and SAT increased with age to levels similar to those in non‐GH‐treated patients (n = 14). In the obese group, adiponectin decreased as VAT increased (r = –0.35, P = 0.11). Leptin (r = 0.67, P < 0.001) and resistin (r = 0.45, P = 0.04) showed positive correlations with SAT. Total cholesterol, low‐density lipoprotein, and triglyceride levels correlated negatively with adiponectin (r = –0.59, r = –0.56, r = –0.56, respectively, P < 0.05) and hemoglobin A1c (r = –0.42, P = 0.08). To maintain lower VAT and prevent cardiovascular disease risk factors, GH treatment may be advisable even in adult patients with PWS. © 2012 Wiley Periodicals, Inc.     

寻常型银屑病患者皮损中瘦素、脂联素与血管内皮生长因子表达的研究

目的 探讨寻常型银屑病患者皮损中瘦素、脂联素及其血管内皮生长因子vascular endothelial growth factor(VEGF)的变化与意义.方法 ①采用免疫组织化学技术检测34例寻常型银屑病患者和21例正常人皮损中瘦素、脂联素与VEGF表达水平.结果 同正常对照组相比,寻常型银屑病患者皮损中瘦素、脂联素以及血管内皮生长因子表达明显高于正常对照组,强阳性率分别为17.65%、17.65%和14.7%,差异有统计学意义(χ2=9.18,P<0.05;χ2=13.34,P<0.05;χ2=18.9,P<0.05),相关分析表明,瘦素表达水平与VEGF表达水平呈正相关(r=0.8,P<0.05),脂联素与VEGF无相关(r=-0.02,P>0.05).结论 寻常型银屑病患者皮损中瘦素、脂联素及其VEGF水平的变化可能与银屑病的发病有关,而血管内皮生长因子的变化可能与瘦素有关.     

Plasma adipokine and inflammatory marker concentrations are altered in obese, as opposed to non-obese, type 2 diabetes patients

Elevated plasma free fatty acid (FFA), inflammatory marker, and altered adipokine concentrations have been observed in obese type 2 diabetes patients. It remains unclear whether these altered plasma concentrations are related to the diabetic state or presence of obesity. In this cross-sectional observational study, we compare basal plasma FFA, inflammatory marker, and adipokine concentrations between obese and non-obese type 2 diabetes patients and healthy, non-obese controls. A total of 20 healthy, normoglycemic males (BMI <30 kg/m²), 20 non-obese (BMI <30 kg/m²) and 20 obese (BMI >35 kg/m²) type 2 diabetes patients were selected to participate in this study. Groups were matched for age and habitual physical activity level. Body composition, glycemic control, and exercise performance capacity were assessed. Basal blood samples were collected to determine plasma leptin, adiponectin, resistin, tumor necrosis factor α (TNFα), interleukin-6 (IL-6), high-sensitivity C-reactive protein (hsCRP) and FFA concentrations. Plasma FFA, inflammatory marker (hsCRP, IL-6, TNFα), adipokine (adiponectin, resistin, leptin), and triglyceride concentrations did not differ between non-obese diabetes patients and healthy, normoglycemic controls. Plasma FFA, IL-6, hsCRP, leptin, and triglyceride levels were significantly higher in the obese diabetes patients when compared with the healthy normoglycemic controls (P < 0.05). Furthermore, plasma hsCRP and leptin levels were significantly higher in the obese versus non-obese diabetes patients (P < 0.05). Significant correlations between plasma parameters and glycemic control were observed, but disappeared after adjusting for trunk adipose tissue mass. Elevated plasma leptin, hsCRP, IL-6, and FFA concentrations are associated with obesity and not necessarily with the type 2 diabetic state.     

Adipocytokine Levels in Obese and Non-obese Subjects: an Observational Study

We evaluated the levels of some inflammatory adipocytokines in 363 obese and 365 non-obese subjects. We measured: body mass index (BMI), waist circumference (WC), fasting plasma glucose, fasting plasma insulin (FPI), homeostasis model assessment (HOMA) index, blood pressure, lipid profile, retinol binding protein-4 (RBP-4), vaspin, omentin-1, leptin, interleukin-6 (IL-6), visfatin, resistin, adiponectin (ADN), adipsin, tumor necrosis factor-α (TNF-α), and high sensitivity C-reactive protein (Hs-CRP). We observed higher BMI, WC, FPI, HOMA index, TC, LDL-C, RBP-4, leptin, IL-6, adipsin, Hs-CRP, vaspin, resistin and TNF-α levels, and lower visfatin, and ADN levels in obese compared to non-obese subjects. Higher WC correlated with lower ADN and visfatin levels, and higher vaspin levels. Higher HOMA index correlated with higher resistin, adipsin, RBP-4, and leptin concentrations, while higher leptin levels correlated with higher TNF-α, Hs-CRP, and IL-6 concentration, and lower ADN values. We confirmed obese subjects’ predisposition to develop dysmetabolic disease and hormonal dysfunctions.     

Change in adipocytokines and ghrelin with menopause

OBJECTIVES: To determine if ghrelin and adipocytokine (leptin, adiponectin, resistin) levels vary with menopause stage or with estradiol (E2), testosterone (T), follicle-stimulating hormone (FSH) and sex hormone-binding globulin (SHBG) concentrations measured in three stages of the menopause transition. METHODS: A study of adipocytokines and menopause was nested in a population-based, longitudinal study of Caucasian women [Michigan Bone Health and Metabolism Study (MBHMS)]. Annual serum and urine samples, available from the MBHMS repository, were selected to correspond to the pre-, peri-, and postmenopause stages of the menopause transition. Participants included forty women, stratified into obese versus non-obese groups based upon their baseline body mass index, who had specimens corresponding to the three menopause stages. RESULTS: Mean resistin levels were approximately two times higher during premenopause compared to peri- or postmenopause. There were significantly lower adiponectin and higher ghrelin levels in the perimenopause stage, compared to either the pre- or postmenopause stage. Increases in FSH concentrations were significantly and positively associated with higher leptin in non-obese women (P<0.01) but not in obese women (P<0.23). Increases in FSH concentrations were also significantly (P<0.005) and positively associated with higher adiponectin concentrations but were negatively associated with ghrelin concentrations (P<0.005). Associations remained following adjustment for waist circumference, waist circumference change, chronological age, and time between measures. CONCLUSIONS: Menopause stages and underlying FSH changes are associated with notable changes in levels of the metabolically active adipocytokines and ghrelin and these changes may be related to selected health outcomes observed in women at mid-life.     

Body composition and serum levels of adiponectin, vascular endothelial growth factor, and interleukin-6 in patients with rheumatoid arthritis

Aim. To investigate differences in body composition and body mass index (BMI) in patients with rheumatoid arthritis (RA) and their correlations with serum production of adiponectin, interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF). Methods. The study included 83 patients (age 53±5 years) with RA treated with methotrexate. We determined their BMI, fat mass, and fat-free mass using bioimpedance analysis, and serum concentrations of adiponectin, VEGF, and IL-6 using immunoassay analysis. Results. Normal BMI was found in 39 (47%), overweight and obesity in 26 (31%), and underweight in 18 (22%) patients. Concentration of adiponectin was lower in overweight/obese patients than in patients with normal BMI (2.1 [0.8-3.9] μg/mL vs 8.9 (7.2-11.3) μg/mL). In underweight patients, it was moderately increased (12.7 [9.3-14.8] μg/mL) and the correlation between the concentrations of adiponectin and IL-6 was positive (r=0.4; P=0.01). Concentrations of VEGF and IL-6 were increased in all groups with RA. The overweight/obese group showed a negative correlation between the concentrations of adiponectin and VEGF (r=- 0.34; P=0.04), a positive correlation between VEGF concentration and fat mass (r=0.39; P=0.02), and a negative correlation between adiponectin concentration and fat mass (r=- 0.23; P=0.02). Conclusion. Inflammatory and angiogenesis activation was found in RA patients with all types of body composition, but only in those with obesity and overweight there was a direct antagonism between adiponectin and VEGF. Further research is needed to identify possible regimens of metabolic correction in different variations of body composition.     

急性胰腺炎患者血浆leptin．和血清hs-CRP、IL-6和IL-8检测的临床意义

目的：探讨了急性胰腺炎患者血浆leptin和血清hs-CRP、IL-6和IL-8水平的变化及临床意义。方法：应用放射免疫分析和免疫比浊法对30例急性胰腺炎患者进行了血浆leptin和血清hs．CRP、IL-6和IL．8检测,并与35名正常健康人作比较。结果：急性胰腺炎患者血浆leptin和血清hs-CRP、IL-6和IL-8水平均非常显著地高于正常人组（P〈0．01）且急性胰腺炎患者血浆leptin和血清hs-CRP、IL-6和IL．8水平呈正相关（r=0．6132、0．5786、0．6218,P〈0．01）。结论：检测急性胰腺炎患者血浆teptin与血清hs．CRP、IL-6和IL-8水平的变化对观察病情发展及预后判断均有重要的临床价值。