荧光分光光度法测定尿中微量碘

尿样用ＳｅｐｈａｎｅｘＧ－１０小柱分离，收集含Ｉ－部分，然后用Ｉ－催化砷－铈氧化还原反应和标准添加直线外推荧光分光光度法测定。本法最低检出限０．０１ｍｇ／Ｌ，尿样中Ｉ－含量在０．００１～０．０３μｇ范围内，批内相对标准偏差＜４％，批间相对标准偏差＜６％，尿样加标回收率８８％～１０４％，用本法测定１０人份随意尿样，Ｉ－浓度范围为０．０７５～０．２１３ｍｇ／Ｌ     

肺癌 胸膜炎 结核性胸膜炎血清及胸水中铜锌测定

采用火焰原子吸收分光光度法（ＦＡＡＳ）检测了５８例肺癌、细菌性胸膜炎和结核性胸膜炎血清中铜锌含量,检测了３０例三组患者胸水中铜锌含量。结果显示,血清和胸水中铜含量与对照组比较,三组患者均升高,且以肺癌组最显（Ｐ＜０．０１）,血清和胸水中铜变化呈平行关系,但以胸水的变化为大。血清和胸水中锌含量各组间无显著差异（Ｐ＞０．０５）。提示,在三组患者的诊治上应注重血清和胸水中微量元素的变化     

中药中7种微量元素的测定

用原子吸收分光光度法测定莲子、麦冬、山药、甘草、女贞子、当归６种中药中的Ｆｅ、Ｚｎ、Ｃａ、Ｎａ、Ｋ、Ｍｎ、Ｃｕ７种微量元素的含量，回收率在９２．５％～１０５％，ＲＳＤ为０．２％～３．３％，结果满意。     

降雪污染锌测定条件研究

本文采用测定微量锌的高灵敏试剂，１．５—二（２′—羟基—５′—氯苯）３—腈苯甲月替（ＨＣＰＣＦ）有效地以胶束增溶离子缔合剂苯基代十四烷基三甲基氯化铵（Ｚｅｐｈ）与锌—ＨＣＰＣＦ络阴离子，形成离子对配位化合物〔１〕的分光光度法，测定降雪污染锌。标准回收...     

γ-氨基丁酸与脑梗塞头痛

本研究采用放射受体分析法测定大脑中动脉区脑梗塞（ＣＩ）急性期１８例头痛患者与２２例非头痛患者和２８例对照组血清和脑脊液（ＣＳＦ）γ－氨基丁酸（ＧＡＢＡ）含量。结果显示：ＣＩ急性期头痛患者和非头痛患者ＣＳＦ中ＧＡＢＡ含量均显著高于对照组（Ｐ＜０．００１），ＣＩ头痛患者ＣＳＦ ＧＡＢＡ水平显著低于非头痛患者（Ｐ＜０．０１），ＣＩ头痛组、非头痛组和对照组三组间血清ＧＡＢＡ含量比较无显著差异（Ｐ＞０．０５）。研究结果提示：ＧＡＢＡ参与ＣＩ头痛的病理生理机制，ＧＡＢＡ类药物可能对ＣＩ头痛有治疗作用。     

心绞痛与血清微量元素锌铜含量相关性的研究

对各类心血管疾病引起的心绞痛４２例，对照组２５例用原子吸收分光光度法测定血清锌铜含量。结果：心绞痛发作时血清锌含量与对照组比较明显降低，血清铜则明显增高，两者皆有极显著差异（Ｐ＜０．０１）。     

双波长分光光度法测定α—萘乙酸及其降解产物

本文用双波长紫外分光光度法同时测定α—萘乙酸及其降解产物α—萘甲醛的含量。实验表明：α—萘乙酸和α—萘甲醛在０．５—５０ｍｇ／Ｌ的浓度范围，对于波长２３２，２５２，２８１，２９８ｎｍ的吸收均满足比耳吸收定律，方法的回收率在９６．４％—１０３．３％，检出限为０．５ｍｇ／Ｌ。对实际试样的分析取得满意的结果，相对误差小于±０．６％。     

反复呼吸道感染儿头发微量元素及体液免疫指标的对比观察

对６１例反复呼吸道感染患儿及对照组１５３例，用日立１７０—３０型原子吸收分光光度计测定了发Ｚｎ、Ｃｕ、Ｆｅ含量；并测定了血清ＩｇＧ、ＩｇＡ、ＩｇＭ。提示复感患儿发Ｚｎ低，发Ｃｕ高，ＩｇＧ与ＩｇＡ均低于对照组。均有统计学意义（Ｐ＜０．０５～０．０１）。     

锌钙镁与血脂及载脂蛋白关系探讨

报告了８８例中老年干部血清中锌、钙、镁的含量及其与血脂和ＡｐｏＡ－Ⅰ和ＡｐｏＢ的关系。结果显示，低ＡｐｏＡ－Ｉ／ＡｐｏＢ比值组及高血脂组血清锌含量高于其正常组，差异有高度显著性，Ｐ＜０．００１；血脂正常组的ＡｐｏＢ含量低于不正常组，而ＡｐｏＡ－Ｉ／ＡｐｏＢ比值则高于正常组，Ｐ＜０．０５；相关分析表明，钙与ＡｐｏＡ－Ｉ、ＡｐｏＢ之间均呈显著正相关，锌与ＡｐｏＡ－Ｉ／ＡｐｏＢ比值之间呈显著负相关，ＡｐｏＡ－Ｉ与ＡｐｏＢ、钙与镁之间亦呈显著正相关。提示锌、钙元素与血脂及载脂蛋白有密切关系，适当降低体内锌含量会降低血脂、提高ＡｐｏＡ－Ｉ／ＡｐｏＢ比值     

应用IFAT对湖北省不同疟区低年龄组人群进行流行病学调查

对仙桃，广水，恩施３市的６～１５岁儿童进行间接荧光抗体试验（ＩＦＡＴ）及带虫率调查。结果表明，ＩＦＡＴ阳性率分别为１．８９％，０．７６％，０．３７％，带虫率分别为０．２７％，０、０。ＩＦＡＴ的阳性率明显高于带虫率，结果提示，在低疟区，由于传染源尚水完全根除，低年龄组人群免疫力低下，因此，必须加强重点人群（疟疾病人，流动人口）的管理，防止疟疾暴发流行。     

正常妊娠妇女血清锌及发锌含量的研究

分别测定了8个地区2833例健康非孕及妊娠妇女血清锌、发锌的含量。结果表明,妊娠妇女整个孕期血锌含量平均下降23.8%,发锌下降49%。统计分析表明,血清锌、发锌与某些重要的血清生化指标呈显著正相关,血清锌与膳食锌、低体重儿出生率亦有一定关系。作者认为,孕妇血清锌、发锌含量是评价妊娠期锌营养状况的重要实验室指标,初步建议妊娠期妇女血清锌低限正常参考值为:早期9.18μmol/L、中期8.87μmol/L、晚期8.42μmol/L;发锌:早、中期为110μg/g、晚期为100μg/g。     

Growth in children from the Wosera subdistrict, Papua New Guinea, in relation to energy and protein intakes and zinc status.

In a cross-sectional study of 123 children aged 24-120 mo from the Wosera subdistrict of Papua New Guinea, height, weight, hematocrit, hemoglobin, hair zinc, and presence of malaria were measured. Two 24-h recalls were undertaken in 67 of the children aged 72-120 mo; 52%, 73%, and 76% had energy, protein, and zinc intakes, respectively, less than two-thirds of the FAO/WHO/UNU recommendations. Stunting was prevalent (29%); 16% were moderately wasted. The prevalence of stunting and hair zinc concentrations less than 1.68 mumol/g was gender related; 38% of males vs 20% of females had Z scores for height-for-age (HAZ scores) less than -2 (P = 0.04); 26% of males vs 11% of females had hair zinc less than 1.68 mumol/g (P less than 0.05). Analysis of variance showed that age, sex, hemoglobin, and log hair zinc influenced HAZ scores, depending on the age group; both sex and the log of the hair zinc values were significant factors in the older children. Stunting in Worsera children was related to chronic deficits in energy and protein and was excerbated in the older male children by suboptimal zinc status.     

用化学代谢平衡法观察平衡膳食中锌的生物利用率研究

用原子吸收光谱法测定儿童发锌含量，根据发锌值把儿童分成３ 组（ＤＢ、ＮＬ、ＤＬ） 。其中ＤＢ 组给予平衡膳食，其它两组给予低锌膳食。观察实验前后３ 组儿童发锌值，并在实验最后１ 周，用化学代谢平衡法观察３ 组儿童锌表观吸收值的变化情况。结果显示ＤＢ 组儿童发锌实验前后差异显著，锌的表观吸收量大于其它两组（Ｐ＜ ００５） 。表明平衡膳食改善了儿童的锌缺乏状况，它是防治儿童锌缺乏的行之有效的措施     

Low levels of zinc in hair and blood, pica, anorexia, and poor growth in Chinese preschool children

Zinc concentrations in plasma and hair were measured in 703 children, aged between 1 and 6 yr, and correlated with parameters of physical development. In the first group of 187 children brought to the Child Health Clinic for routine observation there was a positive correlation of hair zinc content and height for age, with an increased prevalence of low hair zinc content in children of shorter stature. A second group of 303 children in nurseries and kindergartens in Beijing exhibited a hair zinc content of 92 micrograms/g, and 34% of these had very low zinc values below 70 micrograms/g. The third group consisted of 213 children who were brought into the outpatient clinic for a variety of complaints, including pica, anorexia, and poor growth; these had significantly lower values of zinc in hair and plasma than well-nourished children and responded to zinc supplementation with improvement of growth and the disappearance of pica and anorexia. These results suggest that the diet consumed by the population studied may be marginal or inadequate in its content of available zinc.     

婴幼儿持续性腹泻体锌水平变化及甘草锌佐治效果观察

目的观察婴幼儿持续性腹泻(Persistent diarrhea,PD)发锌及血锌水平变化;探讨甘草锌佐治婴幼儿PD的有效性。方法测定87例PD婴幼儿和100例健康婴幼儿的发锌、血锌水平,比较其差异及相关性;将87例PD婴幼儿随机分为两组,对照组常规治疗,补锌组在常规治疗基础上加用甘草锌治疗,并观察疗效。结果 PD婴幼儿的发锌、血锌水平低于正常婴幼儿(P0.01),发锌与血锌高度相关(r=0.824,P0.001);补锌组治疗2周后发锌、血锌值含量较治疗前增加(P0.05)。补锌组患儿治疗总有效率(86.3%)明显高于对照组(76.2%),差别有统计学意义(P0.05)。止泻时间补锌组(4.8±2.1)d,短于对照组(6.4±2.9)d,差别有统计学意义(P0.05)。结论持续性腹泻婴幼儿发锌、血锌水平均低于健康儿;发锌检测可以较准确反映出锌营养水平;在常规治疗的基础上辅以甘草锌治疗PD可以缩短腹泻时间,提高临床治愈率,有明显的疗效。     

Calcium, magnesium, iron and zinc in drinking water and status biomarkers of these minerals among elder people from Warsaw region

The aim of this study was to estimate the influence of calcium, magnesium, iron and zinc contents in drinking water on chosen parameters of nutritional status of these minerals in 164 elder people, 75-80 age, living in Warsaw region. Blood, hair and saliva were collected to assess the calcium, magnesium, iron and zinc nutritional status, while the samples of drinking water were collected to determine these minerals in water Mineral concentrations in blood, hair saliva and water were assessment using the atomic spectrophotometer absorption method It was showed that contribution of drinking water to calcium, magnesium, iron and zinc intake was: 15%, 4%, 5%, 9%, respectively. The relationship between the contents of these minerals in drinking water and their levels in the blood, hair and saliva had low correlation coefficients. It probably showed that homeostasis was maintained in the human body and other factors such as demographic or lifestyle factors were important.     

Nutritional status of adolescent girls in regard to zinc, copper, and iron

Adolescent girls in the sixth through eight grades were surveyed for dietary intake and nutritional status in regard to zinc, copper, and iron in both the fall of 1975 and the spring of 1976. The adolescents (183 girls in the fall and 184 girls in the spring) consumed on the average 60 and 75% of the Recommended Dietary Allowances for iron and zinc, respectively. The mean concentration of zinc in hair samples was 216 +/- 64 microgram/g in the fall and 191 +/- 36 microgram/g in the spring. The mean concentration of copper in hair samples was 31 +/- 23 microgram/g. None of the girls had a concentration of zinc in their hair samples of less than 100 microgram/g. Hair concentrations of zinc anc copper were significantly correlated, but serum and hair concentrations of zinc were inversely correlated. However, in subsample of 102 girls, 3% has serum zinc levels below 70 microgram/100 ml and 4% had serum copper levels below 70 microgram/100 ml. Only 1% of the subjects had a hematocrit below 36%. No significant difference in any of these parameters was noted between girls who had experienced menarche and those who had not.     

Assessment of zinc and copper contents in the hair as the test of environmental pollution of Gliwice

This work presents the zinc and copper content in the hair measured by the flame atomic absorption spectrometric (FAAS). Prior to the FAAS analysis mineralized samples in HNO3+H2O2 (2:1 v/v) solution using microwave technique. Early, all samples washed with water-acetone-water, because grease and dust on the surface of hair may contribute of the element concentrations of hair. Hair samples were collected from people who were not exposed to the contact with copper or zinc. The influence of the variable place of residence, age, sex, was also examined. The mean content of copper was 11.51 +/- 1.27 mg/g dry weight and zinc 146.32 +/- 14.93 mg/g dry weight. The places of residence sex and age were variables that significantly affected copper and zinc concentrations. Men's had more copper and zinc in their hair than women's, whereas hair zinc and copper levels were significantly correlated with the place of the residence and age. The methods were free from interferences, reliable and reproducible. For all metals present in real samples, precision was better than 0.4% (RSD).     

Iron, zinc and copper status in the elderly living in Warsaw district determined by hair analysis

The aim of this study was to assess the iron, zinc and copper status of elderly people using hair trace element levels determined by using atomic absorption spectrophotometry (AAS). The samples of hair were taken in May-June 1999 from 73 people aged 75-80 years (50 men and 23 women) living in Warsaw district. The mean content of iron was 17.32 +/- 12.38 micrograms/g dry weight, zinc 176.44 +/- 64.33 micrograms/g d.w. and copper 11.25 +/- 6.32 micrograms/g d.w. Although these values were in range of reference intervals almost 40% of population had iron and zinc level below them, and for copper the result was even worse (50% of the elderly people). No significant differences between trace element content in hair of male and female have been observed. However it has revealed a trend that female hair contained less iron and more zinc than male hair. The mean copper level was similar for both sexes. It has been found that subject who had not excluded any food products from their diets had higher hair iron contents than those with some dietetic restrictions.     

微波消解-火焰原子吸收法测定儿童头发中Cu、Zn、Ca、Fe的含量

目的探讨微波消解-火焰原子吸收法测定儿童头发中Cu、Zn、Ca、Fe的含量。方法采用微波消解技术,硝酸和过氧化氢彻底分解头发样品,消解后的样品用火焰原子吸收分光光度法测定Cu、Zn、Ca、Fe的含量。结果用微波消解技术-火焰原子吸收法测定儿童头发中Cu、Zn、Ca、Fe,铜浓度在(0～1.0)μg/mL、锌浓度在(0～2.0)μg/mL,钙浓度在(0～20.0)μg/mL,铁浓度在(0～1.0)μg/mL,范围内呈良好的线性关系,相关系数r均大于0.999,0.15≤RSD≤5.66,回收率均在90.0%~105%之间。结论该方法能够满足儿童头发中Cu、Zn、Ca、Fe的分析要求。