右酮洛芬葡辛胺盐的解热镇痛作用

目的:研究右酮洛芬葡辛胺盐(dexketoprofen octylglucamine salt,DKOS)的解热镇痛作用.方法:镇痛实验采用小鼠热板法和醋酸扭体法2种模型;解热作用采用角叉菜胶诱导的大鼠和伤寒、副伤寒菌苗诱导的兔体温升高2种实验模型.结果:DKOS(10,20和40 mg/kg)可明显提高小鼠热板痛阈值;DKOS(2.5,5.0和10.0 mg/kg)可明显减少醋酸引起的小鼠扭体反应及延长扭体反应出现的时间;DKOS(5,10和20mg/kg)对角叉菜胶诱导的大鼠体温升高及DKOS(2.5,5.0和10.0 mg/kg)对伤寒、副伤寒菌苗诱导的兔体温升高均有明显的降温作用.结论:DKOS具有良好的解热镇痛作用.     

辣椒提取物的抗炎镇痛作用

目的 研究辣椒提取物（extract of capsicum,EC)灌胃给药后的抗炎镇痛作用。方法 采用小鼠热板法和热辐射甩尾法两种疼痛模型、小鼠二甲苯诱导的耳水肿和大鼠角叉菜胶诱导的足肿胀两种炎症模型,观察EC灌胃后对痛阈时间和炎症的影响。结果 EC（60、120、240mg/kg)灌胃给药后,能明显延长小鼠热板法和热辐射甩尾法的痛阈时间,对小鼠二甲苯诱导的耳水肿也有明显的抑制作用;且EC（40、80、160mg/kg)能明显抑制大鼠角叉菜胶诱导的足肿胀。结论 EC具有抗炎和镇痛作用。     

茜草总蒽醌的解热镇痛作用

目的　观察茜草总蒽醌的解热镇痛作用。方法　用热板法、电刺激法、扭体法测定茜草总蒽醌 (6 0mg·kg-1灌胃 )对小鼠痛阈值的影响及茜草总蒽醌对伤寒菌苗所致发热家兔的解热作用。结果　多种实验方法均显示茜草总蒽醌明显提高小鼠各时间组的痛阈值 ,并降低伤寒菌苗所致发热家兔的体温。结论　茜草总蒽醌有较强的解热镇痛作用。     

羌板退热方的药效学研究

目的:观察羌板退热方的解热、抗炎、镇痛作用。方法:分别采用啤酒酵母、伤寒Vi多糖疫苗造成动物发热模型,观察本方的解热作用;采用蛋清致大鼠足肿胀、二甲苯致小鼠耳郭肿胀观察其抗炎作用;采用热板法观察其镇痛作用。结果:本方能明显降低啤酒酵母致大鼠发热模型和伤寒Vi多糖疫苗致家兔发热模型的体温,抑制蛋清致大鼠足肿胀及二甲苯致小鼠耳郭肿胀,提高小鼠痛阈。结论:羌板退热方具有显著解热、抗炎、镇痛作用。     

芍芪多苷的解热镇痛作用

目的研究芍芪多苷的解热镇痛作用。方法通过大鼠酵母致热法研究芍芪多苷的解热作用;通过小鼠热板法、扭体法和福尔马林法研究芍芪多苷的镇痛作用。结果芍芪多苷21.3 mg/kgig 1h后可显著抑制酵母诱导的发热大鼠体温的上升,21.3、42.5、85 mg/kgig 2h后各组均可显现降低致热大鼠体温的效应;芍芪多苷120mg/kgig在热板实验中可延长小鼠的舔足潜伏期,30、60、120mg/kgig可显著抑制小鼠的扭体反应,60、120 mg/kgig可减少福尔马林实验第2时相小鼠舔咬注射足的时间。结论芍芪多苷对酵母致热大鼠具有解热作用和一定的镇痛作用,具体机制有待进一步研究。     

金龙利咽口服液解热镇痛的研究

目的：研究金龙利咽口服液抗炎、解热、镇痛等药理作用，方法：观察金龙利咽口服液对小鼠腹腔毛细血管通透性升高，蛋清和甲醛所致大鼠足肿胀，大鼠棉球肉芽肿胀程度的作用，探讨该口服液对小鼠痛阈及三联菌苗所致新西兰兔发热的影响。结果：（1）该口服液对小鼠腹腔毛细血管通透性升高，蛋清和甲醛所致大鼠足肿胀、大鼠棉球肉芽肿亦有不同程度的抑制作用；（2）该口服液提高小鼠的痛阈、对伤寒、副伤寒甲乙三联菌苗所致新西兰兔发热体温有解热作用。结论：金龙利咽口服液具有抗炎、解热、镇痛等药理作用。     

复方可待因镇痛作用的研究

目的 评价复方可待因(可待因：阿斯匹林=1：25)的镇痛作用。方法 镇痛作用采用小鼠热板法，醋酸扭体法和大鼠甩尾法。结果 复方可待因(剂量为可待因14mg／kg、阿斯匹林352mg／kg)可以抑制小鼠由热板和醋酸以及大鼠由光辐射引起的痛反应。结论 复方可待因具有较好的镇痛作用，而且可待因和阿斯匹林在复方中表现出较好的镇痛协同作用。     

飞扬草的镇痛解热和抗炎作用

飞扬草为原生于澳大利亚的野生植物,在所有热带国家生长亦十分普遍。在传统医学上用作镇静、解热和抗炎药。曾报道它的水提物对小鼠有镇静和解焦虑作用。本文旨在进一步研究此提取物的镇痛(小鼠扭体法,热板法)、解热(酵母诱发大鼠体温升高法)和抗炎(角叉菜胶法急性炎症模型和佐药法慢性炎症模型)作用。将干燥全植物粉末(60g)于沸水中浸泡,然后于44℃的热水中浸渍24h;浸渍液过滤后冷冻干燥,提取物(I)收率为14±1％。镇痛作用:(I)对小鼠有中枢镇痛作用。     

香菊软胶囊的主要药效学研究

目的:研究香菊软胶囊的解热、镇痛、抗炎作用,为临床合理使用提供参考.方法:以大肠杆菌内毒素致家兔发热观察其解热作用,以小鼠热板法及醋酸扭体法了解其镇痛作用,以二甲苯致小鼠耳廓肿胀及角叉菜胶致大鼠足趾肿胀观察其抗炎作用.结果:香菊软胶囊可以降低大肠杆菌内毒素所致的家兔体温升高.能提高热板法小鼠的痛阈值,高、中剂量还能减少醋酸法小鼠的扭体次数,表明香菊软胶囊有一定的镇痛作用,且对小鼠和大鼠急性炎症模型均有不同程度的拮抗作用.结论:香菊软胶囊具有较好的解热、镇痛、抗炎作用.     

5-氨基水杨酸片剂的抗炎镇痛作用

目的　研究 5 -氨基水杨酸 (5 ASA)的主要药效学。方法　镇痛试验采用小鼠热板法及扭体法 ;抗炎试验采用小鼠耳片法、蛋清诱导大鼠足肿胀模型。结果　 5 ASA片剂 3种剂量 (75、15 0及 30 0mg·kg-1,ig× 3d)对二甲苯诱导的小鼠耳肿胀度及蛋清诱导的大鼠踝关节肿胀度均有明显抑制作用 ;以上剂量还可明显抑制小鼠扭体反应 ,大剂量还可延长小鼠热板的舔足反应潜伏期。结论　 5 ASA片剂有明显的抗炎镇痛作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.