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甲氧滴滴涕对孕鼠生殖及其胎鼠发育的影响 总被引:2,自引:1,他引:1
目的:研究甲氧滴滴涕(MXC)染毒对孕小鼠生殖和胎鼠发育的影响.方法:将交配成功的雌鼠于孕12~17 d每日给予不同剂量的MXC(0,20,100,200 mg/kg)灌胃,并设芝麻油溶剂为对照组,观察各组孕鼠体质量和黄体等计数测定,同时观察各组F1子代仔鼠的体质量和肛门生殖器距离等,采用放射免疫法检测孕鼠血清雌二醇(E2)和孕酮(P)含量.结果:生殖影响:随染毒剂量增加,母鼠子宫着床数减少,雄性胎鼠肛殖距(AGD)缩短,孕鼠血清中E2和P水平升高,呈不同剂量间差异,具有统计学意义(P<0.05).发育影响:随染毒剂量增加孕鼠体质量增长减少,活胎数下降,不良妊娠结局(死胎、吸收胎数)增多,呈不同剂量间差异,具有统计学意义(P<0.05).结论:MXC可干扰孕小鼠的生殖功能,导致生殖和胎仔发育毒性. 相似文献
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目的:探讨甲氧滴滴涕(MXC)对小鼠骨质疏松的影响.方法:将40只昆明小鼠随机分为50,100,200mg/(kg.d)组及溶剂芝麻油组(对照组),连续14d给予灌胃染毒MXC,末次给予MXC24h内处死小鼠,取肝、脾、胸腺称质量,取左侧股骨进行骨组织形态学观察,并取右侧股骨进行骨干质量、骨钙及骨羟脯氨酸(B-HOP)的测定和比较.结果:不同剂量MXC分别致小鼠骨干质量减少,骨横短径减少,骨钙及骨羟脯氨酸含量减少和胸腺萎缩.结论:MXC可引起小鼠以骨量丢失为主的骨质疏松. 相似文献
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目的:研究甲氧滴滴涕(MXC)对小鼠着床期子宫钙结合蛋白D9k(Calbindin-D9k)基因表达的影响。方法:将真孕及假孕小鼠于孕1-3d(发现阴栓为孕0日)每日给予不同剂量的MXC(0,100,250,500mg/kg)灌胃,对照组(MXC 0mg/kg)只给芝麻油溶剂。采用RT-PCR检测各组孕鼠子宫Calbindin-D9k基因mRNA的表达;免疫组化染色和图像分析技术检测Calbindin-D9k蛋白表达。结果:①孕鼠经MXC染毒3d后,250,500mg/kg MXC组孕鼠子宫Calbindin-D9k mRNA的表达量与对照组相比有显著降低(P〈0.05),然而,100mg/kg MXC组Calbindin-D9k mRNA的表达与对照组相比虽有所减低,但无统计学意义(P〉0.05);②Calbindin-D9k蛋白在孕5日主要表达在子宫内膜上皮细胞和腺上皮细胞。与mRNA的表达水平一致,250,500mg/kgMXC组孕鼠子宫Calbindin-D9k蛋白的表达量与对照组相比显著降低(P〈0.05),而100mg/kg MXC组Calbindin-D9k蛋白的表达与对照组相比无明显差异(P〉0.05)。结论:MXC可以降低小鼠着床期子宫Calbindin-D9k基因的表达,这可能是其干扰胚胎着床的一个毒性机制。 相似文献
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目的探讨孕早期持久性暴露有机污染物甲氧滴滴涕(methoxychlor,MXC)对孕鼠胎盘组织雌激素受体α(estrogen receptorα,ERα)表达的影响。方法孕鼠随机分为3组,每组10只,其中孕鼠皮下注射MXC为染毒组,皮下注射芝麻油为正常对照组,皮下注射雌二醇(estradiol,E2)为阳性对照组。应用半定量逆转录-聚合酶链反应(RT-PCR)技术检测各组孕鼠胎盘组织中ERα的表达强度。结果孕鼠MXC染毒组ERαmRNA(0.68±0.11),与正常对照组(0.49±0.09)比较,有显著性差异(P<0.05);与阳性对照组(0.60±0.10)比较,差异无显著性(P>0.05)。免疫组化结果显示,MXC染毒孕鼠胎盘组织ERα阳性表达明显高于正常对照组。结论 ERα中的高表达可能是MXC影响胚胎发育的毒性作用机制之一。 相似文献
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甲氧滴滴涕对大鼠雌性性腺的毒性作用 总被引:1,自引:0,他引:1
目的:通过亚慢性甲氧滴滴涕(MXC)染毒,观察MXC的雌性性腺毒性.方法:使用雌性SD大鼠作为实验动物,3mo龄,随机分为4组,每组10只,染毒剂量分别为0(对照)、16(低剂量)、32(中剂量)、64(高剂量)mg/(kg.d),对照组给予等剂量芝麻油,所有实验大鼠均通过腹腔注射染毒,连续20d.每天用阴道脱落细胞涂片法连续观察并记录动情周期.染毒结束后于动情期将大鼠处死,观察指标包括体质量,卵巢、子宫的脏器系数; 运用光镜计数各级卵泡及黄体数目.结果:①染毒前后各组体质量增加未见显著差异; ②中高剂量染毒组动情期延长,周期数减少; ③中高剂量组卵巢体质量及卵巢指数显著降低; ④中高剂量组闭锁卵泡显著增加,且次级卵泡闭锁率显著升高; 结论:①甲氧滴滴涕可以使动情周期延长,卵泡闭锁增加,对雌性性腺产生毒性; ②低剂量组未对雌性性腺产生明显的影响. 相似文献
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目的:观察MXC对未成熟小鼠卵母细胞体外成熟的毒性作用;方法:1、用不同浓度MXC培养液培养未成熟小鼠卵母细胞;对照组直接用M2培养液培养,各组培养时间均为24h。2、激光共聚焦观察有丝分裂纺锤体的形成。3、培养结束时,在镜下观察MXC对卵丘扩展的影响。结论:MXC对小鼠卵母细胞的毒性作用可通过直接作用于卵母细胞,使其有丝分裂纺锤体形成异常,从而抑制卵母细胞核的成熟。还可能通过抑制卵丘细胞的扩展,来影响成熟卵母细胞质量,进而影响后续胚胎发育,造成流产。 相似文献
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目的:探讨甲氧滴滴涕(MXC)对小鼠卵巢颗粒细胞增殖和凋亡的影响。方法:MTT法测定卵巢颗粒细胞在MXC作用后的细胞活力(A值);流式细胞技术检测细胞凋亡变化;电镜观察细胞的形态学改变等;综合评价MXC对卵巢颗粒细胞增殖和凋亡的影响。结果:1MTT法测得MXC在(2.5~10.0)μg/ml浓度范围内能明显抑制卵巢颗粒细胞的增殖,并具有浓度依赖性,10.0μg/mlMXC作用36h的卵巢颗粒细胞A值最低;2流式细胞仪检测结果显示5.0,10.0μg/mlMXC处理组卵巢颗粒细胞的凋亡率分别为27.8%和41.7%,明显高于对照组的9.9%(P<0.05);3电镜下可见卵巢颗粒细胞呈典型凋亡样改变。结论:MXC能明显抑制小鼠卵巢颗粒细胞增殖,并具有显著促进颗粒细胞凋亡的作用。 相似文献
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甲氧滴滴涕对雄性生殖系统的氧化损伤作用 总被引:4,自引:1,他引:4
目的观察甲氧滴滴涕(MXC)对雄性小鼠的生殖系统毒性作用并探讨其毒性作用的可能机制.方法光镜观察喂服MXC小鼠睾丸和附睾的变化,并以生物化学的方法测定氧化损伤相关指标的改变.结果精子活动度和活率降低,畸形率增高,生精小管上皮变薄;睾丸和附睾的谷胱甘肽过氧化物酶(GSH-Px)活力和超氧化物歧化酶(SOD)活力显著下降(p<0.001),丙二醛(MDA)含量明显升高(P<0.01),维生素E组各项指标的变化均较21 d组的变化小(P<0.05).结论MXC对雄性小鼠生殖系统具有一定的毒性作用,可导致睾丸和附睾抗氧化能力下降,脂质过氧化增加,其毒性作用可被维生素E减轻. 相似文献
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甲氧滴滴涕对小鼠卵母细胞体外成熟的影响 总被引:1,自引:0,他引:1
目的:观察甲氧滴滴涕(MXC)对小鼠卵母细胞体外成熟的影响.方法:实验组小鼠未成熟卵用7.25,14.5,29ms/L MXC不同浓度培养液培养;对照组小鼠未成熟卵直接用合成人输卵管培养液培养,两组培养时间均为24 h.观察各组生发卵泡破裂(GVBD)和第一极体(PBl)的形成,出现PBl的卵母细胞视为核成熟.结果:培养24 h时,卵母细胞GVBD发生率7.25 ms/L MXC组为80.4%,14.5 ms/L MXC组为78.8%,29 ms/L MXC组为78.4%,与对照组的86%相比较并没有明显改变.培养过程中不同浓度MXC试验组GVBD发生较同期对照组相比较也没有明显延迟.但在培养液培养24 h时PBl的排出率7,25 mg/L MXC组为52.8%,14.5 mg/L MXC组为44.4%.29 mg/L MXC组为22%,明显低于对照组的71.2%,差异具有统计学意义(P<0.05).结论:MXC对体外培养的小鼠卵母细胞GVBD发生没有影响,但能抑制其卵母细胞的核成熟. 相似文献
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目的:探讨甲氧滴滴涕(MXC)对雌性小鼠子宫内膜促血管生成素。1表达的影响。方法:成年雌性小鼠经口染毒MXC,随机分为50,100,200mg/(kg·d)3个染毒组和溶剂芝麻油组(对照组)。采用免疫组织化学及电子计算机图像分析技术,检测染毒后小鼠子宫内膜中ANGPT-1的表达情况。结果:MXC染毒组与正常内膜相比,ANGPT-1呈高表达状态,差异有显著性(P〈0.01)。MXC50,100,200mg/(kg·d)组内膜与正常内膜均随月经周期呈周期性改变,MXC100,200mg/(kg·d)组内膜ANGPT-1在增殖期的表达强度高于分泌期,与正常内膜相比,差异有显著性(P〈0.01);而MXC50mg/(kg·d)组内膜与正常内膜相比,ANGPT-1在增殖期的表达强度无差异(P〉0.05)。结论:MXC染毒后小鼠子宫内膜中ANGPT-1的高表达可能在子宫内膜疾病的发生、发展中起重要作用。 相似文献
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In order to observe the effect of Bushenantai recipe on the expression of endometrial leukemia-inhibitory factor (LIF) in mice with embryonic implantation dysfunction (EID), 120 Kunming mice post coition were randomized into three groups: normal control group, model group and traditional Chinese medicine group (TCM group) (n=40 in each group). Uterus was collected on the pregnancy day (Pd) 4, 5, 6 after an intravenous injection of Evan's blue. The endometrium was dyed by Evan's blue and the mean points of response were observed on Pd 5. The expression of LIF mRNA and protein was detected by RT-PCR and immunohistochemistry respectively and analyzed statistically by image system. The results showed that the number of implantation sites in model group was remarkably less than in normal control group and TCM group. There was no significant difference between normal control group and TCM group. The expression of LIF mRNA and protein in model group was delayed. Bushenantai recipe could increase the expression of LIF mRNA and protein in endometria of mice with EID. It was suggested that Bushenantai recipe could improve embryo implantation of mice with EID by promoting the endometrial LIF expression and endometrial decidualization. 相似文献
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目的研究去甲二氢愈创木酸(NDGA)对小鼠胚泡植入的影响及其作用机制。方法孕2d小鼠随机分4组(A、B、C和对照组),A、B、C组分别经皮下注射1.0、3.0、9.0mg/kg的NDGA,对照组注射等量PBS溶液,孕4d处死一半,取子宫免疫组化检测基质金属蛋白酶-9(MMP-9)表达,透射电镜分析子宫内膜结构的变化;另一半于检孕8d处死,测子宫脏器系数,观察胚胎着床数。结果与对照组比较:A组子宫脏器系数和妊娠胚胎数无显著差异,B、C组子宫脏器系数显著下降,妊娠胚胎数明显减少;A组子宫内膜MMP-9阳性表达差异无显著性,而B、C组MMP-9阳性表达明显减少;A组子宫内膜细胞超微结构无显著变化,B、C组细胞超微结构均遭受不同程度的损伤。结论NDGA可能通过对子宫内膜细胞直接毒性作用干扰MMP-9的表达,抑制胚泡植入。 相似文献
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Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice 总被引:1,自引:0,他引:1
Objective: To explore the therapeutic mechanism of Bushen Yiqi Hexue recipe (BYHR), and observe its effect on pinopodes expression
on endometrial surface in mifepristone induced embryo implantation dysfunctional mice.Methods: Pregnant Kunming mice were randomly divided into the normal group, the control group and the treated group. Mice in the
treated group were administered with BYHR, and those in the normal group and the control group were administered with normal
saline, starting from the very first day of pregnancy (Pd1). On the Pd4, mifepristone (RU486) was subcutaneously injected
into mice in the control group and the treated group. Pinopodes expression on endometrial surface at 09:30–10:00 pm on Pd4
(regarded as time point 1, T1) and at 09:30–10:00 am on Pd5 (as time point 2, T2) was determined by scanning electron microscopy.
Pregnancy rate and average implanted embryos were observed on Pd7.Results: Pregnancy rate and average implanted embryos in the control group were obviously lower than those in the normal group (allP<0.01), while the two parameters were significantly higher in the treated group (P<0.05,P<0.01, respectively) than those in the control group. In the normal group, abundant developing pinopodes were distributed
over the whole endometrial surface in T1, and they were altered to a great deal of fully developed pinopodes in T2. But in
the control group, only a few pinopodes were expressed locally on endometrial surface in T1, showing a nonsynchronous figure
of development, and they completely disappeared in T2. In the treated group, a lot of developing pinopodes were expressed
like those in the normal group but somewhat lagged behind, whereas many fully developed pinopodes were expressed in T2.Conclusion: The decrease of the pinopodes in Tl and the cleaning up of them in T2 is possibly the partial mechanism of mifepristone
in inducing embryo implantation dysfunction. It is indicated that BYHR could improve pinopodes expression on endometrial surface,
eventually better the uterine receptivity and improve the embryo implantation.
Project supported by the National Natural Science Foundation of China (No. 30171193) 相似文献
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目的:研究ME491/CD63对小鼠胚胎体外发育及着床的影响,以及ME491/CD63mRNA及蛋白在假孕D1~8小鼠子宫中的表达规律.方法:①将小鼠8-细胞胚胎培养于含不同浓度ME491/CD63抗体的培养液中,观察胚胎发育,记录囊胚形成及脱透明带的情况.②妊娠D4小鼠宫角注射ME491/CD63抗体,于妊娠D8观察小鼠胚胎植入数量.③用RT—PCR及免疫组织化学技术观察ME491/CD63mRNA及蛋白在假孕小鼠子宫的表达规律.结果:①ME491/CD63抗体可影响胚胎在体外的发育.与对照组相比,在ME491/CD63抗体浓度为1:400及以上时,囊胚的形成率明显降低(P〈0.05);在抗体浓度为1:800及以上时,胚胎脱带率显著下降(P〈0.05),ME491/CD63抗体对胚胎发育的影响具有浓度依赖性.②宫角注射一定量的ME491/CI)63抗体可以显著提高小鼠胚胎的着床数[(5.8±2.3)vs(4.1±1.8),P〈0.05].③ME491/CI)63mRNA在假孕D1~8小鼠子宫中均有表达,于D6表达最丰富.MFA91/CD63蛋白在假孕D1—8的子宫腺上皮细胞均有表达,假孕D1~5腔上皮细胞无表达,假孕D6—8,子宫腔上皮细胞阳性表达.结论:①ME491/CD63在小鼠胚胎发育和着床过程中发挥重要作用.②ME491/CD63在妊娠小鼠子宫的表达是非胚胎依赖性的,其表达量的变化可能与胚胎存在与否有一定关系. 相似文献