茶多酚对炎性牙周膜细胞生物活性的影响

目的观察茶多酚对内毒素脂多糖(LPS)作用的人牙周膜细胞增殖和碱性磷酸酶活性的影响,为茶多酚在牙周疾病的防治中提供一定依据。方法体外培养人牙周膜细胞,采用MTT比色法观察茶多酚对LPS作用的人牙周膜细胞增殖活性的影响,通过碱性磷酸酶(ALP)活性测定法观察茶多酚对人牙周膜细胞的分化作用。结果100μg/ml LPS可抑制人牙周膜细胞的增殖活性和碱性磷酸酶活性。加入LPS同时分别给予50～1100μg/ml不同浓度茶多酚,能对抗LPS的抑制作用,增强人牙周膜细胞的增殖活性和碱性磷酸酶活性,其中在800μg/ml作用24h时促进作用达到最强。结论茶多酚可对抗内毒素对人牙周膜细胞的毒性作用,促进细胞的增殖和骨向分化。     

脂多糖对人牙髓细胞的作用

通过测定牙髓细胞的DNA含量、蛋白质含量和碱性磷酸酶活性来评价脂多糖对人牙髓细胞的影响。 作者将新拔人牙冲洗,纵切获牙髓。采用含一定抗生素的Eagle氏MEM液清洗后取少量培养孵化。用热酚—水法从感染根管分离到的3种细菌即牙龈卟啉菌AE_(12),牙髓卟啉菌AE_(51)和核梭杆菌AE_(91)提纯脂多糖,并以大肠埃希氏菌0111:B_4脂多糖作对照。然后再将浓度为1.10和100μg/ml脂多糖各2ml溶液分别加到人牙髓细胞饲养盘上,测定细胞中DNA含量,蛋白质含量和碱性磷酸酶活性。 结果显示3种细菌的脂多糖在1μg/ml浓度时对DNA和蛋白质的含量均无影响,10μg/ml时均引起DNA含量增加,100 μg/ml使DNA含量减少。10μg/     

IGF-1通过PI3K/AKT信号通路促进牙髓细胞增殖及碱性磷酸酶活性的研究

目的:研究IGF-1对牙髓细胞增殖和碱性磷酸酶(ALP)活性及PI3K/AKT信号通路的影响。方法:采用酶消化法分离培养原代人牙髓细胞。Western blot检测牙髓组织中IGF-1蛋白表达,不同浓度的IGF-1处理牙髓细胞7d,CCK8法检测细胞增殖。用IGF-1(100 μg/L)及LY294002(10 μmol/L)分别单独或同时处理牙髓细胞,培养7 d后MTT实验检测细胞增殖;在培养第3、5、7、14天时检测细胞ALP 活性;在培养第7天时用Western blot检测AKT和p-AKT蛋白表达情况。结果:IGF-1在牙髓炎组织中低表达, IGF-1在20~100 μg/L浓度范围内从作用第3天开始能够显著促进牙髓细胞增殖(P<0.05或P<0.01),且具有剂量和时间依赖效应。LY294002能够抑制牙髓细胞的增殖和碱性磷酸酶活性,具有时间依赖性。IGF-1能促进p-AKT蛋白表达,而 LY294002能减低IGF-1对p-AKT蛋白表达的促进作用。结论:IGF-1可以促进牙髓细胞增殖和碱性磷酸酶活性,且具有浓度和时间依赖性,作用机制可能与PI3K/AKT信号通路相关。[关键词] 牙髓细胞 细胞增殖 碱性磷酸酶     

内毒素脂多糖促进人牙髓成纤维细胞凋亡及其对caspase-3活性的影响

目的观察内毒素脂多糖(lipopolysaccharide,LPS)对体外培养的人牙髓成纤维细胞的致凋亡作用及其对凋亡相关的半胱氨酸蛋白酶3(caspase-3)活性的影响。方法不同浓度(0μg、100μg、200μg、400μg/mL)的LPS作用细胞,采用噻唑蓝比色法检测LPS对细胞增殖的影响;Hoechst33258荧光染色法观察细胞凋亡;流式细胞术检测细胞周期及凋亡;化学比色法检测细胞凋亡过程中caspase-3活性的变化。结果不同浓度LPS组细胞的生长与对照组相比均受到显著的抑制(P<0.01),细胞增殖抑制率呈剂量依赖关系;Hoechst33258荧光染色显示,给药组部分细胞呈典型的凋亡表现;流式细胞术检测结果显示,不同浓度的LPS作用细胞48h,细胞的凋亡率分别为9.2%,23.8%,31.4%;caspase-3活性检测显示,其活性呈剂量依赖性增高,LPS浓度达到400μg/mL时,其活性为对照组的3.4倍。结论LPS可显著地抑制人牙髓成纤维细胞增殖、诱导细胞凋亡、升高caspase-3活性,并呈明显的量效关系。     

变形链球菌超声提取物对牙髓细胞增殖和天然免疫受体基因mRNA表达的影响

目的:观察变形链球菌超声提取物对牙髓细胞的增殖活性、天然免疫受体基因mRNA表达的影响。方法:采用组织块法原代培养牙髓细胞,通过不同浓度的变形链球菌超声提取物(1、10、100μg/ml)作用于牙髓细胞,MTT法检测牙髓细胞的增殖活性;荧光定量PCR检测牙髓细胞NOD2、TLR2和TLR4 mRNA的表达水平。结果:1μg/ml变形链球菌超声提取物处理24 h可促进牙髓细胞的增殖;10μg/ml和100μg/ml处理24 h和48 h可明显抑制细胞增殖。变形链球菌超声提取物(10μg/ml)作用于牙髓细胞后,NOD2、TLR2和TLR4 mRNA表达量均增加。结论:变形链球菌超声提取物可抑制牙髓细胞的生长,促进NOD2、TLR2和TLR4 mRNA的表达。     

蜂胶对人牙周膜成纤维细胞作用的实验研究

目的:探讨蜂胶水提液对人牙周膜成纤维细胞(HPDLFs)生长增殖、骨向分化的影响,寻找蜂胶促增殖及分化作用的最佳浓度。方法:将不同浓度的蜂胶水提液加入体外培养的HPDLFs中,设置阳性对照和空白对照,通过检测HPDLFs的增殖(MTT法)、碱性磷酸酶活性及骨钙素含量,观察蜂胶水提取液对HPDLFs的增殖和分化的影响。结果:10~150μg/ml浓度的蜂胶水提液对体外培养HPDLFs的增殖均有促进作用,100μg/ml浓度时促增殖作用最明显。10~200μg/ml蜂胶水提液均可提高HPDLFs的碱性磷酸酶活性、促进HPDLFs的骨钙素合成(P<0.05),其中以100μg/ml蜂胶水提液作用最显著(P<0.01)。结论:低浓度的蜂胶水提液能促进HPDLFs增殖和向成骨细胞方向分化。     

牙髓卟啉单胞菌超声提取物对成骨细胞周期和凋亡的影响

目的:观察牙髓卟啉单胞菌超声提取物对成骨细胞周期和凋亡的影响。方法:用不同浓度牙髓卟啉单胞菌超声提取物(1、10、100μg/ml)作用成骨细胞,MTT法检测细胞增殖情况,流式细胞仪分析细胞周期分布和凋亡变化。结果:牙髓卟啉单胞菌超声提取物显著抑制成骨细胞的增殖。且在一定时间和浓度范围内具有浓度和时间依赖性。10μg/ml和100μg/ml提取物作用24 h时,处于G1期的成骨细胞明显增多;在作用48 h时,牙髓卟啉单胞菌提取物以浓度依赖方式促进成骨细胞凋亡。结论:牙髓卟啉单胞菌超声提取物可阻滞细胞于G1期,并诱导细胞凋亡,抑制成骨细胞增殖。     

内毒素对人牙髓、牙周膜成纤维细胞DNA含量的影响

具核梭杆菌和牙髓类杆菌内毒素脂多糖(LPS)在50μg/ml浓度下可使人牙髓,牙周膜成纤维细胞核面积值和DNA含量升高,而100μg/ml浓度则可使细胞DNA含量降低,内毒素在不同浓度对细胞DNA含成的影响有显著差异。     

牙周优势菌内毒素对人牙周膜细胞增殖和碱性磷酸酶活性的影响

目的：研究牙周优势菌牙龈卟啉菌、中间普氏菌、具核梭杆菌的内毒素对人牙周膜细胞（PDL细胞）增殖和碱性磷酸酶活性（ALP）的影响。方法：采用MTT比色试验及酶动力学方法，测定PDL细胞的增殖和ALP活性。结果：内毒素在10μg/mL、100μg/mL高浓度时，可显著抑制PDL细胞增殖，而在0.01μg/mL、0.1μg/mL低浓度时，则促进PDL细胞增殖；在10μg/mL、100μg/mL可呈浓度依赖性方式抑制PDL细胞ALP活性。结论：内毒素对牙周膜细胞的抑制作用主要和其浓度有关，不同来源内毒素差异并不显著；内毒素可能通过影响PDL细胞功能而影响牙周组织的代谢和修复过程。     

白细胞介素-10对体外培养人牙囊细胞增殖和碱性磷酸酶活性的影响

目的:研究白细胞介素-10(Interleukin-10,IL-10)对体外培养人牙囊细胞增殖和牙囊细胞碱性磷酸酶活性的影响。方法:体外培养人牙囊细胞,取生长状态良好的第5代细胞,用四甲基偶氮唑蓝法(MTT法)检测IL-10对细胞增殖的作用;用碱性磷酸酶试剂盒检测IL-10及其抑制剂对细胞碱性磷酸酶活性的作用。结果:0、1、10、25、50、100ng/mLIL-10对人牙囊细胞的增殖影响无显著性差异(P>0.05);10ng/mLIL-10作用0、1、3、5、7d对人牙囊细胞的增殖影响无显著性差异(P>0.05)。10ng/mLIL-10作用3～7d降低人牙囊细胞的碱性磷酸酶活性(P<0.05),10ng/mLIL-10抑制剂作用5～7d增加人牙囊细胞的碱性磷酸酶活性(P<0.05)。结论:IL-10对人牙囊细胞的增殖无影响。IL-10降低人牙囊细胞的碱性磷酸酶活性,说明IL-10抑制人牙囊细胞向成骨方向分化。     

眶周骨折复视原因分析:附68例报告

目的：总结分析68例眶周骨折出现复视的病例，分析其原因，为临床处理提供参考。方法：68例眶周骨折伴复视的患者，分别由口腔颌面外科、眼科医生检查，通过X线平片或CT片综合分析，明确复视的原因。结果：68例中，男性多于女性，以竖性复视为主，一般都有眶腔结构的改变。结论：上颌骨、颧骨、鼻骨等部位的骨折，复视的原因主要是眶周解剖的改变。临床医生一定要高度重视，及时予以治疗。     

眶周骨折伴复视的CT评价

目的 :探讨外伤性眶周骨折伴复视的CT诊断价值。方法 :回顾性对照分析 68例 ( 70只眼 )眶周骨折伴复视病例的CT图象和专科检查、临床资料 ,进行定位诊断和骨折分型。结果 :70只眼中眶底爆裂性骨折 45只眼 (其中 15只眼合并眶内侧壁骨折 ) ,眶底非爆裂性骨折 10只眼 ,鼻眶骨折 5只眼 ,颧骨骨折 5只眼 ,眶上部骨折 5只眼。CT检查诊断眶周骨折的正确率可达 10 0 %。 68例中 64例临床出现垂直复视 ,4例为水平复视。结论 :CT可以正确诊断眶周骨折部位并分型 ,帮助鉴别产生复视的原因 ,为临床提供科学依据     

114 例眼眶骨折分析

目的:探讨眼眶骨折的诊断和治疗原则。方法:回顾分析了114例眼眶骨折的患者,分类统计其临床表现和治疗方法。结果:眼球运动障碍、复视和眼球内陷发生率分别为63.2%、62.3%和59.6%;视神经损伤、泪道损伤和眼球破裂的发生率分别为15.8%、15.8%和12.3%。95.6%的患者接受了眶壁整复和人造骨植入术;72.8%的患者进行了骨折复位内固定术;部分患者进行了视神经减压术、眼球修补术和泪道手术。结论:眼球运动障碍、复视和眼球内陷是眼眶骨折的主要临床表现,视神经损伤、眼球破裂和泪道损伤亦不能忽视;眶壁整复和人造骨植入术是眼眶骨折治疗的主要术式,非单纯性眼眶骨折还需行骨折复位内固定术。     

Minimization of zygomatic complex fracture treatment.

The aims of this non-randomized prospective study were to establish and justify minimized therapy for zygomatic complex fractures. Fifty-two consecutive patients were examined and classified with conventional routine radiographs. Preoperative symptoms were recorded. Treatment of zygomatic fractures was by percutaneous hook reduction and miniplate fixation along the frontozygomatic suture. Exploration of the orbital floor was carried out only in cases of primary diplopia or comminuted fractures. Postoperatively, patients were followed for 12 months. Clinical and radiologic assessment of reduction was symmetric and stable in all cases. Preoperative symptoms disappeared completely except for sensitivity disturbance in five patients. Patients with no post-trauma diplopia did not develop eye motility disturbances or enophthalmos. The treatment of a zygomatic fracture is possible by the described technique. Exploration of the orbital floor is indicated only in cases of preoperative diplopia. Therefore, a patient with a zygomatic fracture and diplopia should be classified as having a orbitozygomatic fracture. In cases of comminuted fractures, exploration of the orbital floor remains mandatory.     

128例眶-上颌骨-颧骨复合骨折的临床分析

目的：讨论眶-上颌骨-颧骨（orbital maxillary zygoma,OMZ）复合骨折的特点,以及治疗方法的选择,总结影响OMZ骨折疗效的因素。方法：对华西口腔医院1989年以来收治的128例OMZ骨折进行临床回顾性研究。结果：128例眶-上颌骨-颧骨复合性骨折中,25.8%伴有眶部并发症,对症处理后症状大多消失,8例复视患者眶底或眶周行自体骨整复或Medicon种植体整复,症状消失。83例采用微型夹板坚强内固定,其中4例采用可吸收小夹板。11例陈旧性骨折采用正颌外科技术恢复其面形及咬功能。结论：OMZ骨折应早期复位、坚强内固定,恰当的软组织处理等是影响治疗效果的主要因素。     

下睑缘切口在眶下壁骨折内固定术中的应用

目的:评价下睑缘切口在眶下壁骨折内固定术中的应用价值.方法:在下睑缘睫毛下约2mm处平行于下睑缘作弧形切口,于眼轮匝肌深面与眶隔浅面之间向眶下缘分离,暴露眶下壁骨折部位,解除骨折线内嵌顿软组织,复位后用弧形钛板固定,细线缝合皮肤.结果:103例眶下壁骨折患者骨折愈合良好,无复视和眼球内陷等并发症.89例切口瘢痕平整、隐蔽、无缺陷;5例轻度下睑外翻,9例局部小皱褶.结论:下睑缘切口在眶下壁骨折内固定术中切口隐蔽,操作简便,创伤小,术野清晰,便于复位和固定.     

Straightforward factors for predicting the prognosis of blow-out fractures

In blow-out fractures, some nonoperative cases have a poor outcome, and a method for accurate prognosis is required. To address this need, we retrospectively reviewed blow-out fractures presenting at Teikyo University Hospital between July 2004 and May 2007 and conducted a survey regarding diplopia and enophthalmos for nonoperative cases. Computed tomographic scan findings were divided according to fracture width and the degree of protrusion of the inferior rectus muscle into the maxillary sinus. We had 106 patients presenting with blow-out fractures, and 89 patients had been treated nonoperatively. In medial orbital wall fractures, no patient had diplopia, and 1 patient had enophthalmos after nonoperative treatment. In punched-out orbital floor fractures, all cases had diplopia when the fracture width was less than half the diameter of the globe, and the protrusion of the inferior rectus muscle into the maxillary sinus was half or more of its section. Even if the fracture width was less than half the diameter of the globe, 2 of 3 patients had enophthalmos when the protrusion of the inferior rectus muscle into the maxillary sinus was half or more of its section. Among the linear orbital floor fractures, 1 case required an emergency operation. We suggest a new algorithm for treatment of blow-out fractures based on computed tomographic scan findings that can also contribute to making a prognosis.     

Endoscopic Assisted Combined Transantral and Subciliary Approach in Treatment of Orbital Floor Fracture: A Case Report

Management of orbital floor fracture remains the most debated topic in maxillofacial field. There are many approaches to reconstruct orbital floor fractures and restore orbital position and function, but many have the drawback of incomplete visualization, especially of the posterior part of the orbit. Pain, diplopia and enophthalmos are the most common presenting symptoms in patients who sustained orbital blow out fracture. The main aim in treating orbital fracture is to reduce the prolapsed orbital tissue and reconstruct the floor which will improve diplopia and enophthalmos. As minimally invasive surgical techniques are gaining popularity, it is possible to reconstruct the orbital fracture defects using endoscopes. Endoscopic assisted combined transantral and subciliary technique provides better surgical access and outcome in the treatment of orbital floor fracture.     

Less common orbital fracture patterns: the role of computed tomography in the management of depression of the inferior oblique origin and lateral rectus involvement in blow-in fractures.

During the past decade, advances in radiographic imaging have made it possible for the surgeon managing orbital fractures to adopt a rational therapeutic strategy based on a knowledge of alterations in surgical anatomy secondary to traumatic injury. To illustrate the value of computed tomography in the surgeon's armamentarium for management of orbital fractures, cases are presented in which imaging proved decisive in planning a course of therapy. Two patients presented with two types of isolated lateral blow-in fracture, an uncommon fracture pattern. The other cases underscore the value of defining involvement of the inferior oblique origin and lateral rectus muscles in imaging complex orbital fractures, issues not emphasized in earlier literature. Although diplopia alone does not always warrant surgical intervention, diplopia in the context of computed tomography-defined muscle entrapment or muscle origin displacement justifies operative therapy. These cases demonstrate the value of computed tomography in directing surgical therapy with resolution of diplopia and prevention and correction of enophthalmos.     

Evaluation of a computed-tomography-based assessment scheme in treatment decision-making for isolated orbital floor fractures

Introduction

Treatment decisions for fractures of the orbital floor are based on clinical appearance, ophthalmological examination, and computed tomography (CT) scans. In extensive fractures, decisions are easily made between conservative and surgical treatment. However, objective parameters are rare in inconclusive cases.