复方丹参片指纹图谱的质量评价研究

目的：全面评价复方丹参片等复方丹参制剂的质量。方法：HPLC指纹图谱法。结果：利用指纹图谱技术。对市场上的多种复方丹参制剂指纹图谱进行了比较研究，并对其中的主要活性成分进行了定量分析。结论：不同厂家生产的复方丹参片的指纹图谱相差较大，质量各异。     

复方丹参片质量标准的沿革与质控技术的研究进展

综述复方丹参片制法工艺与质量控制标准的沿革,以及近年来对复方丹参片质量控制技术的研究进展;进一步分析其存在问题,并提出应提倡丹参多成分的检测、增加三七指标成分的测定、补充冰片的质量控制标准及加强指纹图谱研究等改进建议。     

止痛化癥胶囊水提中间体指纹图谱研究

目的建立中药复方止痛化癥胶囊水提中间体的HPLC指纹图谱,对不同批次间样品进行质量控制。方法 Agilent ZORBAX SB-Aq C_(18)色谱柱(4.6 mm×250 mm,5 μm);以乙腈-水(含体积分数为 0.05%的磷酸)为流动相进行梯度洗脱,流速:1.0 mL/min;进样量:20 μL;柱温:40 ℃;检测波长:280 nm;利用国家药典委员会发布的"中药色谱指纹图谱相似度评价系统2004A 版"对指纹图谱进行相似度评价,并使用 SPSS25.0软件进行聚类分析。结果建立了中药复方止痛化癥胶囊水提中间体HPLC指纹图谱,确定了30个共有色谱峰,指认出8个色谱峰,10批止痛化癥胶囊水提中间体指纹图谱与对照指纹图谱相似度值均在0.95以上,通过聚类分析可将 10 批样品聚为 4 类。所建立的中药复方止痛化癥胶囊水提中间体HPLC指纹图谱测定方法精密度、重现性及稳定性良好。结论首次建立中药复方止痛化癥胶囊水提中间体的HPLC指纹图谱,从样品生产中间环节控制药品质量,对止痛化癥胶囊的质量评价具有重要的意义。     

复方血栓通颗粒HPLC指纹图谱的研究

目的 建立复方血栓通颗粒的HPLC指纹图谱分析方法,对该制剂进行质量控制.方法 采用Thermo ODS-2 C18色谱柱（4.6 mm×150 mm,5μm）,以乙腈-0.1％（体积分数）H3PO4为流动相进行梯度洗脱,检测波长为270 nm,检测时间为60 min.采用2009年版《中药色谱指纹图谱相似度评价系统》软件,对15批次复方血栓通颗粒指纹图谱的相似度进行评价.结果 15批次复方血栓通颗粒指纹图谱中有14个共有峰,15批样品指纹图谱与对照指纹图谱的相似度均大于0.90.结论 该方法简便、稳定、重复性好,可用于复方血栓通颗粒的质量控制.     

基于指纹图谱及多指标成分定量的丹参提取工艺对比研究

目的 对已上市制剂中丹参不同提取工艺的提取效果进行对比分析.方法 按照冠心丹参胶囊、复方丹参片、复方丹参滴丸、丹参片4种代表制剂的提取工艺制备丹参提取液,研究建立同时测定提取液指纹图谱及多指标含量的HPLC方法,测定不同丹参提取液的丹参素钠、丹酚酸B和丹参酮ⅡA含量及指纹图谱,采用中药色谱指纹图谱相似度评价软件计算指纹图谱的相似度.结果 4种丹参提取液中丹参酮ⅡA、丹参素钠、丹酚酸B含量分别为0.27~1.25、1.44~4.46、33.77~93.92 mg·g-1;冠心丹参胶囊与丹参片、复方丹参滴丸、复方丹参片的指纹图谱相似度分别为0.781、0.853、0.758,复方丹参滴丸与复方丹参片的指纹图谱相似度为0.879.结论 4种代表制剂丹参提取工艺的丹参酮ⅡA、丹参素钠、丹酚酸B含量及指纹图谱均存在明显差异,针对相同的临床适应症,需深入研究以实现丹参工艺的标准化、规范化.     

丹参破壁饮片的质量研究

目的:对丹参破壁饮片质量进行研究,为丹参破壁饮片质量标准的建立提供实验依据。方法:采用BT-9300HT型激光粒度分析仪测定丹参破壁饮片的粒径分布;采用薄层色谱法进行定性鉴别;采用高效液相色谱法测定其丹参酮、丹酚酸的含量,并结合"中药色谱指纹图谱相似度评价系统2012版"软件对各批次丹参破壁饮片指纹图谱进行分析。结果:十批丹参破壁饮片粒径分布范围在30.38～63.44μm之间;薄层鉴别中,各批次样品所显主斑点的颜色和位置与对照药材一致;各批次丹参破壁饮片中三种丹参酮总含量为1.0%～3.5%,丹酚酸B含量为3.0%～4.5%;各批次丹参破壁饮片的指纹图谱考察相似度在0.967～1.000之间。结论:本文所建立的方法稳定、可靠、重复性好,可用于丹参破壁饮片的质量控制和评价。     

牛黄解毒丸红外指纹图谱的研究

目的建立可用于鉴别牛黄解毒丸的红外指纹特征图谱。方法对所有样品采用统一的提取条件,采用丙酮提取并测试其提取物的红外指纹图谱。结果不同厂家多批次牛黄解毒丸具有独特而稳定的红外指纹特征图谱,并与伪品区别明显。结论经数据平均后的牛黄解毒丸的红外指纹特征图谱可以作为其质量控制和真伪鉴别的根据。     

中药指纹图谱研究概况

<正>中药材的栽培种植,中药的生产加工中质量标准的规范化、现代化研究是中药走向世界的前提。采用现代先进手段开展中药的质量评价,应全面反映中药的内在质量,而指纹图谱作为中药材单味药和复方全组成及其提取物的质量控制方法,已经成为国际公认的控制中药和天然药物质量的最有效手段。现将中药指纹图谱的研究概况作简单介绍。1定义及其特点:中药指纹图谱借用DNA指纹图谱发展而来,广义上包括中药材DNA指纹图谱和中药材化学成分指     

中药指纹图谱的研究进展

目前 ,指纹图谱作为中药材单味药和复方全组成及其提取物的质量控制方法 ,已经成为国际公认的控制中药或天然药物质量的最有效手段。现将中药指纹图谱的研究进展作简单介绍。1　中药指纹图谱的发展背景中药指纹图谱 (Fingerprinting)借用DNA指纹图谱发展而来 ,广义的中药指纹图谱包括中药材DNA指纹图谱和中药材化学 (成分 )指纹图谱 ,狭义的中药指纹图谱仅指后者 ,是表达中药代谢产物化学特征的指纹图 ,包括光谱、波谱和色谱指纹图。色谱指纹图谱发展最早 ,也是迄今为止最为重要的指纹图谱。按谢培山先生的定义[1 ] ,中药色谱指纹图谱是…     

中药复方体内指纹图谱研究进展

中药复方的物质基础及作用机制较为复杂,无明确完整的质量控制标准,临床应用时有较高的不良反应发生率,这导致其很难在国际上流通。为此,国内许多学者开始着手研究中药及其复方的质量标准,以期为中药走向世界找到科学的质量控制标准。经过大量的实践证明,中药指纹图谱可以较好地解决中药及其复方制剂的稳定性、药效成分复杂等问题：因此,中药指纹图谱逐渐成为目前国际较为认可的中药及其复方质量控制的评价模式,但其仍不能完全有效表征中药在体内的生物活性及其作用机制。而将指纹图谱技术和中药血清药物化学、中药血清药理学相结合则有助于明确中药复方在体内的作用成分和作用机制。为此,国内外许多学者开始运用体内指纹图谱来研究中药复方制剂在体内的有效成分和作用机制。本文就近年来中药复方体内指纹图谱的研究进展作一综述。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.