探讨临床尿液常规检验分析前质量控制对策与效果

目的 探讨临床尿液常规检验分析前质量控制对策与效果,为临床提供重要参考.方法 选取2018年3月至2019年3月本院接收的200例行尿液常规检验的住院患者,通过随机数字表法分为对照组和实验组,各100例.实验组患者给予分析前质量控制,对照组患者给予尿液检查常规控制.比较两组患者检验标本检验不合格情况、检验满意度、分析不...     

显微镜与尿分析仪在尿常规检查中的结果分析

目的：探讨尿液分析仪法与尿沉渣显微镜检测法在尿常规检查中的价值。方法：采集2010年5月～2012年2月住院患者的新鲜晨尿1 000份,用Mejer-600型尿11项分析仪和尿沉渣显微镜检测法同时进行检测,并对结果进行比较分析。结果：1 000例尿液检验标本经尿液分析仪分析与光学显微镜检查,两者结果均为阴性890例,相符率89%;两者结果不相符110例,占11%。结论：尿沉渣显微镜检查仍然是一种方法简便、结果最可靠的检查方法,也是最主要的参考方法。尿液分析仪法和尿沉渣显微镜检测法相结合在尿检中可提高检测的准确率。     

优利特URIT-1280全自动尿液分析系统与显微镜检测法在尿常规检验中的临床应用价值

目的探讨全自动尿液分析仪与显微镜检测法在尿常规检验中的临床应用价值。方法选择2017年1月在我院接受尿常规检验的367例门诊及住院患者为研究对象,留取患者晨尿为送检标本,分别应用全自动尿液分析仪、显微镜镜检、尿干化学法进行检测,统计分析3种检查结果。结果与全自动尿液分析仪和显微镜检查相比,干化学法在红细胞和白细胞阳性检出率较低,差异有统计学意义(P<0.05);尿沉渣全自动分析仪与显微镜检测法的检查结果比较,差异无统计学意义(P>0.05)。结论进行尿常规检查时,应用全自动尿液分析仪与显微镜检查有较高的临床应用价值,可将2种检测方法联合应用,以提高日常尿常规检验水平。     

尿沉渣与尿常规在尿液检验中的相关性分析

目的对尿沉渣与尿常规在尿液检验中的相关性进行分析探讨。方法选取2017年4月至2017年10月本院采集的200份尿液标本作为研究对象,均接受尿沉渣检查(采用显微镜检测方法)以及尿常规检查(采用尿液分析仪进行检测),对两种检查方法的结果进行比较分析,探讨尿沉渣与尿常规检查方法在尿液检验中的相关性。结果两种检查方法的结果显示,有181份的检查结果相符,均为阴性,符合率为90.5%,其余19例的检验结果不符,占比为9.5%。结论尿液分析仪操作简单,效率高,但是容易受到多种因素的影响而使检测结果的准确率受到影响,因此还不能完全取代尿沉渣检查法,为了提高检验结果的准确性,需要二者结合使用,从而为临床医生提供更为准确的参考依据。     

临床尿液常规检验分析前质量控制对策与效果观察

目的：探讨临床尿液常规检验分析前质量控制对策与效果。方法选择2014年1月—2015年12月间,我院收集到的尿常规检验标本146份,将其按照时间顺序分成2组,每组73份,2014年1月—12月间收集的标本定义为对照组,2015年1月—12月间收集的标本定义为观察组。对照组标本进行常规尿液检查控制,观察组标本在尿液常规检验分析前进行质量控制,对2组检验结果进行对比分析。结果所有标本均顺利完成检查,检验结果可靠,观察组检验结果符合率为98.63%,较对照组的89.04%显著升高（P<0.05）。结论在临床尿液常规检验中实施分析前质量控制,可使检验结果准确性得到显著提高,值得关注并推广。     

研究尿液检验中尿沉渣与尿常规的相关性

目的研究分析尿液检验中尿沉渣和尿常规的相关性。方法选择我院2016年1月至12月采集的尿液标本400例,选择Uritest-200B尿11项分析仪实施尿常规检查,同时选择显微镜进行尿沉渣检测,对尿常规检查结果和尿沉渣检查结果进行对比分析。结果在尿蛋白,红细胞,白细胞的阴性率、阳性率方面,尿沉渣检查、尿常规检查比较差异无统计学意义(P0.05);综合尿沉渣和尿常规的检查结果后,红细胞,白细胞,尿蛋白的符合率分别为96.0%(384/400)、99.0%(396/400)、98.0%(392/400),均具有较高的符合率。结论在尿液检验中,尿沉渣检查、尿常规检查的相关性表现为检查准确率基本保持一致,各自具有自身的优点和不足,有效结合尿沉渣检查和尿常规检查,能让检查的准确率显著提高,值得临床推广和应用。     

阴道分泌物对尿常规检查结果的影响分析

目的 分析阴道分泌物对尿常规检查结果的影响,为临床检验工作提供有价值的参考信息.方法 选取接受尿常规检查妇科患者98例,采用随机数字表法分成对照组与观察组,分别采用常规尿液采集法和采取0.02%呋喃西林溶液对会阴部实施清洗后实施标本采集.对2组患者的尿常规检查结果进行对比分析.结果 观察组患者白细胞和红细胞的检出率均较对照组显著降低(P<0.05);观察组患者尿液标本中均未检出上皮细胞、蛋白质,对照组中上皮细胞、蛋白质检出率分别为4.08%和22.45%.结论 阴道分泌物会对尿常规检验结果产生一定程度的影响,从而增加了检测阳性率,在今后的临床检验工作中应对其给予足够的重视.     

尿液潜血临床检验分析

目的 用尿液分析仪潜血反应和尿液显微镜检查红细胞两种方法 检验尿中红细胞的数量与形态的结果 进行分析对比.方法 对400例患者尿液标本进行尿常规检测和尿沉渣显微镜检查.结果 两种检查结果 有一定的差异.结论 临床工作中尿液分析仪潜血反应与显微镜检查红细胞应联合应用,对于肾损伤的确定,鉴别及病程中动态观察有一定意义.     

浅谈临床尿液常规检验分析前的质量控制

目的探讨分析临床尿液常规检验分析前的质量控制。方法将我院收治的360例患者的尿常规检验标本作为研究对象,收集与回顾性分析门诊患者和住院患者的尿常规检验标本,统计与分析患者尿常规检验标本数量、检验不合格率以及不合格标本产生原因及分布。结果接受尿常规检验标本总数为360例,不合格标本10例(2.78%),门诊患者接受尿常规检验标本为308例,不合格标本4例(1.30%),住院患者接受尿常规检验标本为52例,不合格标本6例(11.54%);患者尿常规检验不合格标本产生原因中,主要原因为标本数量少4例(1.11%),其中主要为住院患者2例(3.85%)。结论临床尿液常规检验不合格标本多来自于住院患者,标本数量少是最主要原因,次要原因包括标识错误、申请单填写不详、标本污染以及容器错误等,应加强临床尿液常规检验分析前的质量控制,以减小尿常规检验不合格率。     

阴道分泌物对尿常规临床检查结果的影响及其处理方法探讨

目的 分析女性阴道分泌物对尿常规检查结果的影响,探讨合理有效的尿常规检查操作方法.方法 回顾分析120例患者尿常规临床资料,所有患者均采集2次尿液,2次采集尿液的方法不同,根据采集尿液的方法分为对照组和试验组,每组120例.对照组由患者本人用常规尿液采集方法对中段尿液进行采集,试验组在责任护士协助下通过采取合理方法阻止阴道分泌物流出后,再进行中段尿液采集,比较2组标本的检验结果.结果 试验组检查结果中蛋白质、上皮细胞、白细胞、红细胞等各项临床检验指标检出率低于对照组,差异有统计学意义(P＜0.05).结论 女性阴道内分泌物对尿常规检查会产生较大影响;妇科进行临床尿常规检查时需采取有效措施阻止阴道分泌物的流出.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.