两种血细胞分离机分离外周血造血干细胞效果比较

目的　比较FenwalCS 3000plus及CobeSpectra两种血细胞分离机分离外周血造血干细胞的效果。方 法　共有76名供者入组。FenwalCS 3000plus组31例,平均年龄(39.1±12.9)岁,共循环38次;CobeSpectra组 45例,平均年龄(38.2±11.2)岁,共循环65次。比较两组采集物白细胞总数、单核细胞百分率、采集物单核细胞中 CD34+细胞百分率。结果　两组采集物的白细胞计数无明显统计学差异;FenwalCS 3000plus组单核细胞百分率 高于CobeSpectra组。两组采集物中CD34+细胞百分率无明显统计学差异。CobeSpectra组采集物中单核细胞百 分率与白细胞数负相关。两组单核细胞百分率、两组白细胞数均与CD34细胞百分率无相关性。结论　本组资料 显示在分离外周血干细胞方面FenwalCS 3000plus与CobeSpectra之间没有差异。     

3种血细胞分离机采集外周血造血干细胞的安全性与有效性研究

目的 初步探讨3种血细胞分离机在采集外周血造血干细胞中的应用,并分析影响其安全性与有效性的相关因素.方法 总结2005年10月～2012年1月,浙江省血液中心与浙江大学附属第一医院自/异体外周血造血干细胞采集129例,比较并探讨CS-3000 Plus、COM.TEC与COBE Spectra MNC3种血细胞分离机的采集效率与影响因素,评估3种仪器对供者血小板与血红蛋白的影响.结果 129例供者中,CS-3000 Plus血细胞分离机采集47例,共70次;COM.TEC血细胞分离机采集22例,共33次;COBE Spectra MNC血细胞分离机采集60例,共89次.每位供者平均采集1.5次.3种仪器采集的产品CD34+细胞总数无显著性差异,COBE Spectra MNC组体外总循环量与采集产品容量最低,对供者的血小板影响程度最低(P ＜0.005);COM.TEC组对供者血红蛋白的影响最小(P ＜0.005);采集产品中CD34+细胞总数与采集前供者MNC计数和处理血量均呈正相关关系(r =0.771 4,0.397 1,P＜0.001).结论 根据不同供者的循环血量、动员情况、血色素、血细胞计数、患者经济状况等因素选择合适的单采仪器与方法,经过1～2次的单采都可以安全地采集到高浓度的、能满足临床需要的外周血造血干细胞.相比之下,COM.TEC血细胞分离机采集外周血造血干细胞产品容量大,对供者血小板影响也大.     

Amicus与CS-3000血细胞分离机采集外周血造血干细胞效果比较

目的 探讨Amicus与CS-3000血细胞分离机采集外周血造血干细胞的效果.方法 供者均给予G-CSF动员,分别采用Amicus血细胞分离机的MNC程序和CS-3000血细胞分离机的单个核细胞程序采集供者外周血干细胞;用流式细胞仪检测所采集干细胞的CD34+细胞数.供者52例,共采集62次,其中Amicus血细胞分离...     

CS-3000PLUS血细胞分离机采集37名供者外周血造血干细胞效果的分析

近年来，异基因外周血造血干细胞移植技术发展迅速并广泛应用于临床，是治疗多种血液病和实体瘤的有效方法，但采集物的特性和数量直接影响移植的效果。我们对CS-3000plus血细胞分离机应用单个核细胞（MNC）采集程序采集干细胞效果进行了观察和分析，现报告如下。     

血细胞分离机采集干细胞效果观察

目的探讨Amicus血细胞分离机的MNC程序采集外周血干细胞的效果。方法用Amicus血细胞分离机的MNC程序采集健康供者和肿瘤患者外周血干细胞;供者给予G-CSF动员,患者采用化疗加G-CSF动员。用流式细胞仪检测CD34~+抗原表达细胞数。供患者共采集了53次,处理(8±2)个循环,处理抗凝全血(11420±2401)ml,时间(236±28)min,抗凝剂(957±195)ml。结果采集CD34+细胞(235.26±298.53)×10~6,MNC的采集效率为(53.05±39.03)%;患/供者采集前外周血CD34~+计数>0.04×10~9/L(n=28),采集CD34~+细胞为(4.94±4.57)×10~6/kg;而当患/供者采集前外周血CD34~+计数<0.04×10~9/L(n=25),采集CD34~+细胞为(1.07±0.64)×10~6/kg;所采集的干细胞制品中血小板含量为(5.57±4.26)×10~(10)/袋。单采后患/供者Plt、Hb、Hct分别下降14.79%,12.21%和12.23%,所有程序没有观察到严重的副反应。结论Amicus血细胞分离机的MNC采集程序能安全地采集到血小板含量低、高产量的异体和自体CD34~+细胞。     

血细胞分离机采集外周血干细胞的相关影响因素分析

<正>自体外周血干细胞(Autologous peripheral blood stemcell,APBSC)移植已成为治疗多种白血病和实体瘤以及组织修复治疗的有效方法。用于血液病移植治疗时细胞用量大,干细胞动员时间长;用于再生修复治疗时的细胞数量相对较少,这类患者骨髓功能无异常,对动员剂较敏感,可视个体情况而定。针对如何提高采集效率,探索采集的有效方法,总结分析影响因素,现报告如下。     

2种血细胞分离机外周血干细胞采集效率的比较

目的 比较CS-3000 plus和COM.TEC血细胞分离机采集外周造血干细胞的效率.方法 56例检测标本均来自本院2011年自体外周血干细胞移植患者或异基因移植供者,其中CS-3000血细胞分离机采集33例,COM.TEC血细胞分离机采集23例.比较2组采集前供/患者的白细胞计数、红细胞压积;产品中白细胞数、单个核细胞比例、体外总循环量、产品体积、产品中MNC(单个核细胞总数)及单位循环量MNC、CD34+细胞总数及单位循环量CD34+.结果 CS-3000 plus与COM.TEC血细胞分离机采集产品中单个核细胞的比例有一定差异(P＜0.05),CS-3000 plus中单个核细胞比例高于COM.TEC血细胞分离机;CS-3000 plus产品体积显著小于COM.TEC 血细胞分离机产品体积(P＜0.05);按照单位循环量采集的细胞数,无论是以MNC还是CD34+细胞计算,2种机器采集效率的差异无统计学意义(P＞0.05),提示2种血细胞分离机均可采集足够的造血干细胞.结论 CS-3000plus和COM.TEC血细胞分离机在单个核细胞采集效率和CD34+细胞采集效率的差异无统计学意义.     

间断流动离心式血细胞分离机采集自体外周血造血干细胞9例分析

20世纪90年代以来，自体外周血干细胞移植治疗实体瘤几乎完全取代自体骨髓移植，在白血病的自体移植中也占据越来越重要的地位。国内已对此进行了多项研究探讨，但所用分离机多为连续流动式血细胞分离机，有关间断流动离心式血细胞分离机采集、冻存外周血造血干细胞的资料甚     

Peripheral blood stem cell collection with a blood cell separator

Forty-three patients with malignant nonmyeloid diseases underwent peripheral blood stem cell collections on an apheresis system (Spectra, COBE BCT, Lakewood, CO). Collections took place during the white cell (WBC) recovery phase following conditioning chemotherapy. One hundred two procedures were done after chemotherapy alone, and 72 procedures after chemotherapy plus granulocyte-colony-stimulating factor (G-CSF). Four centrifugal separation factors were tested. One and one-half patient blood volumes were processed in each procedure. The mean volume of the collected component was 158 +/− 16 mL. After chemotherapy alone, the procedures provided a mean of 0.8 × 10(8) WBCs per kg and 2.3 × 10(4) colony-forming units-granulocyte macrophage (CFU-GM) per kg of recipient body weight. The mononuclear cell percentage in the components increased with the centrifugal separation factor from 85 to 96 percent. In parallel, platelet contamination increased from 2.1 to 3.8 × 10(11). The collect hematocrit ranged from 1.0 to 2.5 percent (0.01-0.025). The collection efficiency for mononuclear cells and CFU- GM also increased with the centrifugal separation factors from 52 to 70 percent for mononuclear cells and from 55 to 68 percent for CFU-GM. Collections performed after G-CSF-stimulated mobilization were characterized by a higher neutrophil contamination independent of centrifugal separation factor, which gave a mean mononuclear cell percentage of 64 percent in the collected component. The average yield for these procedures was 2 × 10(8) WBCs per kg and 28 × 10(4) CFU-GM per kg.(ABSTRACT TRUNCATED AT 250 WORDS)     

间断流动离心式血细胞分离机在治疗性血液成分单采术中的应用

治疗性血液成分单采术是指分离和去除患者血液中某些病理性成分 ,还输其正常成分 ,以达到治疗疾病的目的。根据去除成分的不同 ,可分为治疗性血细胞单采术和治疗性血浆单采术。后者因在操作过程中需要一定量的溶液和正常人血浆补充交换已去除的病理性血浆 ,又称血浆置换术[1] 。国内已对此进行了研究 ,但所用分离机多为连续流动式血细胞分离机 [2 ,3 ] ,间断流动离心式血细胞分离机应用于治疗性血液成分单采资料甚少。笔者自 2 0 0 2年 5～ 8月应用间断流动式血细胞分离机进行 5例治疗性血液成分单采 ,现报告如下。材料与方法1　病例　患者 …     

Ex vivo evaluation of PBMNCs collected with a new cell separator

BACKGROUND: This study reports on an evaluation of the ability of a cell separator (Amicus, Baxter Healthcare) and the integral MNC computer software program to collect a variety of MNC subsets. The collection efficiency (CE) of the Amicus for these MNC subsets was compared to that of another cell separator (CS-3000 Plus, Baxter). The collected MNCs were also assayed ex vivo to determine if these cells remained functional. STUDY DESIGN AND METHODS: Healthy volunteer blood donors were recruited to provide PBMNCs for the isolation of CD3+, CD4+, CD8+, CD19+, NK, and gammadelta+ cells and monocytes. Cells were collected with an Amicus (test arm; n = 16) or a CS-3000 Plus (control arm; n = 11) cell separator. Cells were counted on a flow cytometer and CEs were calculated. For functional studies, the Amicus-collected MNC data were compared to CS-3000 Plus historical data. Functional studies performed included surface antigen expression assays (CD8+), proliferation assays (CD4+ and CD8+ cells), NK cytotoxicity assays for K562 and HUVE cells, and E-selectin induction on endothelial cells through NK+ contact dependency. Dendritic cells (DCs) were generated from CD34+ cells collected on the Amicus, positively selected by the use of antibody-bound, magnetic bead technology, and then cultured ex vivo with a combination of growth factors to generate the DCs. RESULTS: CEs were higher on the Amicus than on the CS-3000 Plus for CD3+ (68 vs. 54%), CD4+ (70 vs. 56%), CD8+ (68 vs. 52%), and CD19+ (60 vs. 48%) cells (p<0.05). For the two separators, CEs were equivalent for monocytes, NK+, and gammadelta+ cells. The Amicus separator collected significantly fewer platelets than did the CS-3000 Plus (p<0.00001). CD4+, CD8+, and NK cells proliferated normally. NK cells appropriately stimulated E-selectin expression on endothelial cells. Culture-generated DCs obtained by using Amicus-collected CD34+ cells expressed appropriate cell surface markers. CONCLUSION: The Amicus separator is acceptable for the collection of PBMNC subsets. The device collects CD3+, CD4+, CD8+, and CD19+ T- and B-cell subsets with greater efficiency and collects MNCs with significantly fewer contaminating platelets than does the CS-3000 Plus. Cells collected on the Amicus are suitable for use in a variety of research and clinical immunobiologic studies.     

Peripheral blood stem cell mobilization by cytokines

Hematopoietic stem cells circulate in the peripheral blood. These cells can be collected by apheresis techniques either in the unperturbed state, after mobilization following the administration of cytokines like G-CSF or GM-CSF, or during the phase of early blood count recovery following chemotherapy-induced myelosuppression. The number of cells collected following mobilization is greater than that obtained after apheresis in the unperturbed state. There are, however, qualitative differences between unperturbed and mobilized cells. Chemotherapy related mobilization can be potentially dangerous in that severe myelosuppression necessary to achieve mobilization can have serious consequences. There are no controlled studies that evaluate the relative merits of each method of collection. Regardless of the techniques employed peripheral blood stem cells can reliably accelerate hematologic recovery after potentially myeloblative therapy and provide an alternative to bone marrow support. © 1992 Wiley-Liss, Inc.     

Extended storage of single-donor platelet concentrate collected by a blood cell separator

Use of a sealless blood pathway in a blood cell separator (CS-3000, Fenwal) permits collection of platelets in a "closed system" when saline and anticoagulant solutions are integrally attached; this in turn allows storage of instrument-collected platelet concentrates (PCs) beyond 24 hours. To evaluate extended storage of high yield PCs, cells collected with the instrument were stored (200 ml plasma) for 8 days (flatbed agitation) in either 3-liter polyvinylchloride (PL 146) containers (n = 6), polyolefin bags (PL 732) (n = 8), or two 1-liter polyolefin (double PL 732) containers (n = 8). A mean of 4.45, 4.09, and 3.94 X 10(11) platelets were stored in PL 146, single PL 732, and double PL 732, respectively; total white cells per container averaged 0.3, 0.2, and 0.2 X 10(9) for the three container systems. By day 1, platelet pO2 dropped to 14 and 16 torr in PL 146 and PL 732 PCs (pCO2, 127, and 82 torr). In contrast, double PL 732 maintained high pO2 (approximately equal to 80 torr) and low pCO2 (approximately equal to 30 torr) through day eight. Glucose declined at faster rates in PL 146 and single PL 732 containers, while lactate increased more rapidly (338 and 197 mg/dl of lactate on day four vs. 116 mg/dl for double PL 732 units). Morphology scores dropped from 400 to 98 (PL 146) and 216 (PL 732) at day four (pH values of 6.3 and 7.0), while a score of 330 was seen in double PL 732 PCs.(ABSTRACT TRUNCATED AT 250 WORDS)     

5-day storage of platelets collected on a blood cell separator

One of the earliest devices available for plateletpheresis is the Haemonetics system; this machine has been updated recently to permit the use of software in a closed system and thus storage of the collected platelets beyond 24 hours. The authors examined the in vitro and in vivo function of platelets collected on the Haemonetics AutoSurge machine and stored for 5 days in two separate 1000-mL CLX bags. The average count per bag was 1.8 +/- 0.4 x 10(11) in approximately 200 mL of plasma. Immediately following collection, the platelet response to ADP and epinephrine represented 78 and 35 percent, respectively, of the preapheresis values. Aggregation to single stimuli subsequently decreased to 29 and 0 percent, respectively by Day 5. This response is equal to or better than the response reported with the Fenwal CS-3000, the only other plateletpheresis device routinely used for long-term storage. The pH of the preparations was well maintained throughout storage, and there was little alteration in hypotonic shock response or serotonin uptake. Serotonin release decreased consistently. The morphology scores indicated good maintenance of shape immediately following collection; this subsequently decreased after 5 days of storage. Bacterial cultures were negative in all instances. The 51Cr in vivo survival and recovery was good with 65.5 +/- 7.1 percent recovery and an average survival of 7.3 +/- 1.3 days (multiple hit; n = 5). The data indicate that storage of the Haemonetics plateletpheresis product is feasible and that the product is as good as others currently available.     

应用Cs-3000PLUS血细胞分离机采集外周血干细胞病人的护理

自体外周血干细胞移植（APBSCT）因具有造血和免疫功能重建快、肿瘤细胞污染少等优点,成为治疗多种恶性肿瘤并使病人长期生存的有效治疗方法。APBSCT成功的基础是获得足够数量的自体外周血造血干细胞（APBSC）,除了病人自身因素、动员方法和动员剂外,采集的时机及各参数的设定也影响造血干细胞采集的数量。我院应用Cs-3000PLUS血细胞分离机对42例病人进行自体外周造血干细胞采集分离,均取得了成功。现将采集过程的护理干预介绍如下。     

应用Cs-3000PLUS血细胞分离机采集外周血干细胞病人的护理

自体外周血干细胞移植(APBSCT)因具有造血和免疫功能重建快、肿瘤细胞污染少等优点,成为治疗多种恶性肿瘤并使病人长期生存的有效治疗方法.