老年宫颈鳞癌组织中COX-2和MMP-9的表达变化及意义

目的探讨环氧化酶(COX)-2和基质金属蛋白酶(MMP)-9的表达在老年宫颈鳞癌中,发生发展中的作用评价。方法应用免疫组化SP法检测60例宫颈鳞癌(观察组)、60例正常宫颈组织(对照组)中COX-2和MMP-9的表达水平,并分析COX-2和MMP-9的表达与临床病理特征的关系。结果观察组COX-2和MMP-9表达的阳性率明显高于对照组(P0.01);观察组中COX-2和MMP-9的阳性率与淋巴结转移、肿瘤最大直径及Ki67的表达密切相关。结论 COX-2和MMP-9的高表达在宫颈癌的发生、发展中均起作用,联合检测可能与子宫颈鳞癌的预后密切相关。     

基质金属蛋白酶-2和-9在食管鳞状细胞癌中的过表达

目的 探讨人食管鳞癌组织中基质金属蛋白酶(MMP)-2、-9的表达及其临床意义.方法 采用免疫组织化学法,检测57例食管鳞癌及10例食管正常黏膜石蜡标本中MMP-2、-9蛋白的表达情况.结果 食管鳞癌组织中MMP-2及MMP-9阳性表达率(40.3%,61.4%)显著高于正常组织(0.0%,10.0%)(P＜0.05).食管鳞癌组织中MMP-2及MMP-9阳性表达与淋巴结转移和癌组织浸润深度有显著相关性(P＜0.05),而与患者的性别、年龄和组织分化程度无显著相关性(P＞0.05).结论 MMP-2和MMP-9在食管癌中显著高表达,与食管癌的转移及侵袭有关,其异常表达可能共同参与食管癌的发生、发展过程,检测MMP-2、-9可作为食管癌病理学特点的参考指标.     

COX-2、MMP-9、VEGF在甲状腺肿瘤中的表达

目的 探讨COX-2、MMP-9、VEGF在甲状腺肿瘤中的表达及其临床意义.方法 免疫组化法检测COX-2、MMP-9、VEGF在甲状腺癌、甲状腺瘤、结节性甲状腺肿及正常甲状腺组织中的表达情况,对临床病理特征及其相关性进行对比分析.结果 COX-2、MMP-9、VEGF在甲状腺恶性肿瘤组织中的蛋白表达均明显高于甲状腺良性病变,并且在甲状腺癌中与年龄、包膜完整性、肿瘤大小、临床分期显著相关(P<0.05),COX-2的表达与淋巴结转移之间有显著意义(P<0.05),均与性别无明显关系(P>0.05).COX-2、MMP-9在甲状腺瘤中的表达高于结节性甲状腺肿及正常组织(P<0.05).结论 COX-2、MMP-9和VEGF可作为甲状腺癌重要的生物学参考指标,联合检测有利于提高早期甲状腺癌的诊断率.     

胰腺癌组织中MMP-2、MMP-9的表达

目的 检测胰腺癌组织基质金属蛋白酶( MMP-2,MMP-9)的表达,分析其与临床病理特征的关系.方法 应用免疫组化SP法检测30例胰腺癌及配对癌旁胰腺组织以及11例慢性胰腺炎、6例正常胰腺组织中MMP-2、MMP-9的表达,运用统计软件SPSS 12.0分析其与临床病理特征的相关性.结果 正常胰腺组织均无MMP-2及MMP-9的表达.慢性胰腺炎组织MMP-2、MMP-9的阳性表达率均为18.2％ (2/11).胰腺癌组织MMP-2、MMP-9的阳性表达率分别为63.3％ (19/30)和56.7％ (17/30);配对癌旁组织的阳性表达率分别为23.3％ (7/30)和40.0％( 12/30).胰腺癌组织的MMP-2及MMP-9阳性表达率均显著高于配对癌旁组织(P＜0.05);胰腺癌组织及癌旁组织中MMP-2及MMP-9的表达均显著高于慢性胰腺炎组织和正常胰腺组织(P值均＜0.05).MMP-2、MMP-9的表达与胰腺癌临床分期、肿瘤大小、分化程度及淋巴结转移相关.结论 胰腺癌组织中MMP-2、MMP-9呈高表达,其高表达与肿瘤的恶性程度相关.     

Ang-2在食管鳞癌血管生成中的作用

应用免疫组化SP法检测食管鳞癌及食管断端正常鳞状上皮中促血管生成素（Ang）-2、血管内皮生长因子（VEGF）的表达,以及CD105标记的微血管密度（MVD）。结果显示,Ang-2蛋白表达主要定位于肿瘤细胞的胞质和胞膜,尤其是癌灶边缘血管重建区。食管鳞癌组织中Ang-2和VEGF蛋白表达显著高于食管断端正常鳞状上皮（P〈0．01）,癌组织中Ang-2表达程度与VEGF表达程度及MVD呈明显正相关（P〈0．01,〈0．05）。提示食管鳞癌组织中Ang-2高水平表达可能促进肿瘤新生血管形成．促进食管鳞癌的发生发展。     

基质金属蛋白酶-9和组织金属蛋白酶抑制剂-1在食管鳞癌中的表达

目的探讨基质金属蛋白酶-9（MMP-9）和组织金属蛋白酶抑制剂-1（TIMP-1）在食管鳞癌中的表达及其临床意义。方法用免疫组化和Western blot法分别检测41例食管鳞癌患者的癌及相应正常组织中MMP-9和TIMP-1的表达变化。结果食管鳞癌组织中MMP-9阳性表达率与食管癌淋巴结及静脉转移有关；MMP-9的阳性表达率与表达量均显著高于TIMP-1；MMP-9和TIMP-1的表达呈负相关。结论MMP-9与食管鳞癌的侵袭转移有关，其机制可能与食管鳞癌组织中的MMP-9／TIMP-1平衡失调有关；MMP-9与TIMP-1联合检测有助于食管鳞癌生物学行为的判断。     

Angiopoietin-2,MMP-9和TIMP-1在胰腺癌中的表达及其与胰腺癌侵袭转移的关系

目的观察血管生成素-2(Ang-2),基质金属蛋白酶-9(MMP-9)及其组织抑制因子-1(TIMP-1)在胰腺癌中的表达,探讨3者的表达与胰腺癌侵袭转移间的关系及3者间的联系。方法应用免疫组化染色法检测40例胰腺癌患者癌组织,20例癌旁组织中Ang-2、MMP-9及TIMP-1的表达。结果胰腺癌组织中Ang-2、MMP-9及TIMP-1阳性表达率分别为45%,67·5%及47·5%,均显著高于癌旁组织中表达水平。结论Ang-2、MMP-9在胰腺癌的侵袭与转移过程中起重要作用,并且它们之间可能存在互相诱导或是协同效应,其共位表达可能作为判断预后的指标。TIMP-1的表达随胰腺癌浸润深度增加而减少。     

食管癌组织环氧化酶-2的表达与血管生成的关系

目的:探讨环氧化酶-2(COX-2)在食管癌组织的表达及其与肿瘤血管生成的关系．方法:免疫组化法检测食管鳞癌手术切除标本90例和癌旁正常黏膜34例中COX-2表达,采用抗CD34抗体标记微血管内皮细胞,计算微血管密度(MVD)．分析COX-2表达与MVD及其与食管癌主要临床病理特征的相关性．结果:食管癌组织COX-2阳性表达率为84．4％显著高于癌旁正常黏膜的20．6％(x2=45．47,P =0．00)．COX-2表达与肿瘤细胞分化程度、临床TNM分期和淋巴结转移密切相关,TNM分期中Ⅲ Ⅳ期的食管鳞癌组织中COX-2表达率为92．9％,显著高于Ⅰ Ⅱ期的70．6％(x2= 7．99,P=0．005)．高、中分化的食管鳞癌组织中COX-2表达率为92．9％,显著高于低分化的 70．6％(x2=7．99,P=0．005)．伴有淋巴结转移的食管鳞癌组织中COX-2表达率为94．3％,显著高于无淋巴结转移的70．3％(x2=9．61,P= 0．002)．食管癌组织MVD值为29．68±3．81, 显著高于癌旁正常黏膜的15．12±2．80(t= 20．28,P=0．00)．MVD与肿瘤的TNM分期和淋巴结转移密切相关,TNM分期中Ⅲ Ⅳ期的食管鳞癌组织中MVD值为31．46±3．52,显著高于Ⅰ Ⅱ期的26．74±2．06(t=-7．09,P=0．00)．伴有淋巴结转移的食管鳞癌组织中MVD为 31．72±3．43,显著高于无淋巴结转移的26．76 ±2．01(f=-7．90,P=0．00)．Spearman等级相关分析表明,MVD与COX-2表达呈显著正相关(r =0．607．P=0．00)．结论:COX-2异常表达及其诱导的血管生成在食管癌的侵袭和淋巴结转移中起重要作用．     

甲状腺乳头状癌组织中COX-2、MMP-9的表达及意义

目的检测甲状腺乳头状癌组织中环氧化酶（COX）-2、基质金属蛋白酶（MMP）-9的表达,并探讨其临床意义。方法采用免疫组织化学SP法检测74例甲状腺乳头状癌（有淋巴结转移者39例,无淋巴结转移者35例）患者肿瘤组织及15例癌旁正常甲状腺组织中的COX-2和MMP-9蛋白。结果COX-2和MMP-9蛋白在有淋巴结转移组甲状腺乳头状癌细胞中阳性表达率分别为86．0％和89．7％,在无淋巴结转移组甲状腺乳头状癌细胞中的阳性表达率分别为57．1％和60．0％,二组阳性表达率相比P均〈0．05。结论甲状腺乳头状癌中COX-2和MMP-9蛋白表达上升,且其表达与肿瘤淋巴道转移相关;检测这两种蛋白表达将有助于判断甲状腺乳头状癌的转移潜能及预后。     

COX-2和MMP-2的表达与食管癌侵袭转移的关系

目的探讨COX－2和MMPZ的表达与食管癌侵袭和转移的关系。方洁应用兔疫组织化学（S法）检测COX．2、 ＊＊kZ在45例食管鳞癌，癌旁组织中的表达。结果＊ox之和＊＊P之在食管癌组织中表达的阳性率显著高于癌旁组织（P＜ 0．05）O COLZ和＊M卜2在有外膜浸润组和淋巳结转移组阳性表达率明显高于无外膜浸润组和无淋巴结转移组（P＜0．0到。C012 和＊＊＊2在食管癌组织中的表达密切相关（P＜0刀别。结论食管癌组织中C0lz和＊M卜2的高表达促进了食管癌的侵袭和转 移，COX．2利MMPZ在食管癌组织中的表达有相关性。提示COX．2和MMP毛可作为判断预后的指标和化学治疗的靶点。     

Clinical significance of matrix metalloproteinase-9 expression in esophageal squamous cell carcinoma

AIM: To evaluate the expression of matrix metalloproteinase-9 (MMP-9) and its clinical significance in esophageal squamous cell carcinoma (ESCC). METHODS: The expression of MMP-9 in 208 cases of ESCC was detected by immunohistochemistry (IHC) and its clinical significance in ESCC especially the relationship with the clinicopathological parameters was analyzed. RESULTS: The percentage of positive cases for MMP-9 detected by IHC was 49.0%. MMP-9 was mainly expressed in the cytoplasm of cancer cells especially in the invasive front. Only weak expression was detected in the stromal cells and no expression in non-cancerous mucosa. The expression of MMP-9 was positively correlated with poorer differentiation (P= 0.001<0.01), existence of vessel permeation (P= 0.027<0.05) and lymph node metastasis (P=0.027<0.05). CONCLUSION: The expression of MMP-9 correlates with the cancer cell differentiation, vessel permeation and lymph node metastasis. It may be a novel biomarker for the diagnosis and treatment of ESCC.     

Effects of cyclooxygenase-2 on human esophageal squamous cell carcinoma

AIM:To study the relationship between the cyclooxy-genase(COX)-2 gene and the proliferation and apopto-sis of esophageal squamous carcinoma EC109 cells.METHODS:The techniques of RNA interference(RNAi)and cell transfection,as well as the levels of oncogenic-ity in nude mice,were used to study the role of COX-2 in the esophageal squamous carcinoma cell(ESCC)line EC109.Following RNAi and transfection,Western blot-ting analysis was used to determine the expression of the COX-2 protein.The 3-(4,5-dimethylthiazol...     

MMP-2和MMP-9蛋白在结肠癌中的表达

目的:分析基质金属蛋白酶-2(MMP-2)和MMP-9蛋白与结肠癌病理因素的关系,探讨MMP蛋白在结肠癌发生中的临床意义.方法:用SP免疫组化法检测31例结肠癌中MMP-2和MMP-9蛋白表达情况,并用SPSS10.0forWindows软件统计分析MMP蛋白与临床病理因素的关系.以20例溃疡性结肠炎、21例结肠腺瘤和10例正常结肠黏膜作为对照组.结果:MMP-2除结肠癌组和正常结肠黏膜组间相比具有显著性差异(10.0%vs54.8%,P<0.05)外,其余各组间比较差异无统计学意义,MMP-9各组间比较差异无统计学意义,从溃疡性结肠炎、结肠腺瘤到结肠癌中MMP-2和MMP-9蛋白表达阳性率不同且具有递增趋势.MMP-2和MMP-9蛋白表达与结肠癌的Duke’s分期及有无淋巴结转移显著相关(A B期:38.9%和27.8%;C D期:76.9%和84.6%;无淋巴结转移:38.9%和27.8%;有淋巴结转移:76.9%和84.6%,P<0.05).结论:MMP-2和MMP-9蛋白过度表达对结肠癌的诊断、Duke's分期及有无淋巴结转移判断具有重要的临床意义.     

5-脂氧合酶mRNA在食管鳞癌中的表达及与血管生成的关系

目的探讨5-脂氧合酶（5-LOX）和微血管密度（MVD）在食管鳞状上皮细胞癌（ESCC）组织中的表达及相关关系，及5-LOX与肿瘤浸润和转移的关系。方法用半定量RT-PCR法测定35例ESCC患者癌组织（T）和相邻癌旁组织（N）中5-LOX mRNA的表达，求得T／N值。同时用免疫组织化学法测定癌组织和癌旁正常组织中微血管密度（MVD）的T／N值。结果在35例ESCC患者中，33例5-LOX mRNA的T／N值〉1．0，占94．3％，34例MVD的T／N值〉1．0，占97．1％。5-LOX mRNA和MVD与食管鳞癌的肿瘤大小、浸润深度和淋巴结转移、肿瘤分化程度有关。5-LOX mRNA的T／N值与MVD的T／N值呈正相关。结论5-LOX与食管鳞癌血管生成和肿瘤浸润转移密切相关，其高表达可能促进肿瘤血管生成，从而促进ESCC的浸润和转移。     

Clinicopathologic significance of expression of cyclooxygenase-2 in human esophageal squamous cell carcinoma

BACKGROUND/AIMS: The focus of studies on cyclooxygenase-2 (COX2) have been on its ability to mediate the biological behavior of human tumors including tumorigenesis, tumor progression, apoptosis and differentiation. The aim of the current study was to elucidate a further finding on the clinicopathologic significance of immunohistochemical expression of COX2 in esophageal squamous cell carcinoma. METHODOLOGY: The immunohistochemical expression of COX2 was examined for 68 specimens of esophageal squamous cell carcinoma (ESCC) and the correlation of COX2 expression with clinicopathologic features was examined. COX2 immunoreactivity was weak in 27 (40%) and strong in 41 (60%) of the carcinomas. RESULTS: The proportion of poorly differentiated SCCs among tumors with a strong expression of COX2 (34%, 14 of 41) was significantly higher than among tumors with a weak expression of COX2 (19%, 3 of 14, p = 0.02). The depth of the tumors (p = 0.0004), lymph node metastasis (p = 0.009) and the stage of the tumors (p = 0.001) were advanced significantly more progressively in ESCCs with a strong COX2 expression. Moreover, survival was significantly reduced (p = 0.02) among patients with strong COX2 expression when compared with the COX2 weak group. CONCLUSIONS: This study showed that strong expression of COX2 was correlated with tumor progression and poor differentiation in ESCC.     

缺氧诱导因子-1α与EphA2在食管鳞状细胞癌中的表达及其相关性研究

目的 检测缺氧诱导因子-1α (HIF-1α) 蛋白与EphA2蛋白在食管鳞状细胞癌中的表达定位并探讨二者表达的相关性.方法 采用细胞免疫荧光法检测HIF-1α与EphA2在食管鳞状细胞癌细胞株Eca109中的表达与定位;采用免疫组化法检测HIF-1α与EphA2在41例食管鳞状细胞癌和25例正常食管组织中的表达;常氧和缺氧条件下,Western 印迹法检测食管鳞状细胞癌细胞株Eca109和TE13经RNA干扰(RNAi)技术特异性沉默HIF-1α后EphA2表达的变化.结果 ①细胞免疫荧光结果显示HIF-1α和EphA2均在Eca109细胞胞质表达.②免疫组化结果显示HIF-1α在食管鳞状细胞癌和正常食管组织中的阳性表达率分别为70.73%(29/41)和0%(0/25),两者比较差异有统计学意义(P<0.05).EphA2在食管鳞状细胞癌和正常食管组织中的阳性表达率分别为78.04%(32/41)和28%(7/25),两者比较差异有统计学意义(P<0.05).相关性检验提示HIF-1α和EphA2在食管鳞状细胞癌中表达呈正相关性(r=0.5654,χ~2=13.11,P<0.05).③ Western印迹结果显示在食管鳞状细胞癌细胞株Eca109和TE13中,EphA2表达随HIF-1α的沉默而受抑制.结论 HIF-1α与EphA2在食管鳞状细胞癌组织中呈高表达,且二者表达呈正相关;EphA2表达受HIF-1α的调控,可能为HIF-1α的靶基因.     

Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma

AIM: To investigate the expression of vascular endothelial growth factor-c (VEGF-C) mRNA and microvessel density (MVD) in human esophageal squamous cell carcinoma (ESCC) and its relationship with clinical significance. METHODS: Specimens obtained from 43 patients undergoing surgical resection for ESCC were used in this study. The expression of VEGF-C mRNA was examined by in situ hybridization. Tumor MVD was determined immunohistochemically with anti-CD31 antibody and estimated by image analysis. Ten sections of adjacent normal mucosa were also examined. RESULTS: VEGF-C mRNA expression was detected in cytoplasm of carcinoma cells. Of the 43 ESCC patients studied, 18 cases (41.9%) were positive for VEGF-C mRNA. No VEGF-C mRNA expression was observed in normal esophageal mucosa. VEGF-C mRNA expression correlated significantly with lymph node metastasis, TNM stage and depth of invasion (P < 0.05). Furthermore, histological grade (differentiation) tended to correlate with VEGF-C mRNA expression, but was not statistically significant (P > 0.05). In tumor lesions, the MVD was significantly greater than that in normal esophageal mucosa. MVD correlated significantly with lymph node metastasis, TNM stage and depth of invasion (P < 0.05), but not with histological grade (differentiation) (P > 0.05). Lesions with VEGF-C mRNA expression had a significantly higher MVD than that of those without VEGF-C mRNA expression (P < 0.05). CONCLUSION: VEGF-C plays a role in lymphatic metastasis via lymphangiogenesis and angiogenesis in ESCC. VEGF-C is one of the important predictors of the biological behavior in ESCC.     

Expression and clinical significance of CtBP2 in human esophageal squamous cell carcinoma

<正>Objective To explore the expression of C-terminal binding protein 2(Ct BP2)in human esophageal carcinoma and its relationship with clinicopathological parameters and survival.Methods The expression levels of CtBP2 in eight cases of fresh frozen specimens of esophageal squamous cell carcinoma(ESCC)and the adjacent e-