Effect of Xiongshao Capsule (芎芍胶囊) on Vascular Remodeling in Porcine Coronary Balloon Injury Model

Ｉｔｗａｓｋｎｏｗｎｉｎｇｅｎｅｒａｌｔｈａｔｔｈｅｃｅｎｔｒａｌｋｅｙｏｆｃｏｒｏｎａｒｙｒｅｓｔｅｎｏｓｉｓｆｏｒｍａｔｉｏｎａｆｔｅｒｐｅｒｃｕｔａｎｅｏｕｓｔｒａｎｓ ｌｕｍｉｎａｌｃｏｒｏｎａｒｙａｎｇｉｏｐｌａｓｔｙ（ＰＴＣＡ）ｉｓｔｈｅｉｎｔ     

Protective Effects of Xiongshao Capsule (芎芍胶囊) on Anti-inflammatory Function of High-Density Lipoprotein in An Atherosclerosis Rabbit Model

Objective: To observe the effects of Xiongshao Capsule(芎芍胶囊, XSC) on anti-inflammatory properties of high-density lipoprotein(HDL), myeloperoxidase(MPO) and paraoxonase 1(PON1) in serum of atherosclerosis(AS) rabbit model and explore the anti-inflammatory protective effects of XSC on HDL. Methods: Sixty rabbits were randomized into the control, the model, XSC low-, medium-and high-dose(Rhizoma Chuanxiong + Radix Paeoniae rubra : 0.6+0.3, 1.2+0.6, 2.4+1.2 g·kg-1·day-1, respectively), and simvastatin(1 g·kg-1·day-1) groups. The model rabbits were fed with high-fat diet and respective drugs for 15 weeks. The blood and thoracic aortas samples were collected at the end of 15 weeks. The levels of serum MPO and PON1 as well as total cholesterol(TC) and free cholesterol(FC) in aorta wall cells were tested by enzyme linked immunosorbent assay. Results: TC and FC in the model group were significantly higher than those in the control group(P0.01). Compared with the model group, TC and FC in the XSC groups were significantly lower(P0.05 or P0.01), so was simvastatin group(P0.01). There was no significant difference in PON1 level between groups(P0.05), even between model and control groups(P0.05). The serum MPO level in the model group was significantly higher than that in the control group(P0.05), which was significantly lower in XSC groups as well as simvastatin group(P0.05 or P0.01), and no difference was found between XSC groups and simvastatin group(P0.05). Conclusions: XSC can reduce the serum MPO level in AS rabbits to protect the anti-inflammatory function of HDL, maintaining the normal lipid transport function. TC and FC levels in aorta cells decline, and this process initiated by XSC plays an anti-AS role.     

Effects of Quyu Xiaoban Capsule(祛瘀消斑胶囊) on Vascular Endothelial Function in Patients with Atherosclerosis

Objective: To evaluate the therapeutic effects of Quyu Xiaoban Capsule (祛瘀消斑胶囊 ,QYXBC) on endothelial dependent vascular relaxation (EDVR) function in patients with atherosclerosis (AS)with ultrasonic technique. Methods: Tested were the endothelial function and blood lipids of 42 patients with AS in the treated group and 30 healthy volunteers in the control group. And re-examination of these parameters was carried out on the AS patients after they had been treated with QYXBC for 10 months. Results: Before treatment, the reactive hyperemia induced changes in artery diameter in the treated group was significantly lower than that in the control group (P＜0.01), while insignificant difference was found between the two groups in response to nitroglycerin. In the treated group after treatment, with D%-R improved significantly (P＜0.01), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)decreased by 16.3%, 5.6%, 10.2% respectively and high-density lipoprotein cholesterol (HDL-C) increased by 7.5%. EDVR was correlated negatively with the serum TC, LDL-C concentrations and the baseline brachial diameter (D0) (r = -0.41, -0.66, -0.59, respectively, all P＜0.01), but correlated positively with HDL-C (r = 0.62, P＜0.05). The ameliorative extent of EDVR was correlated positively to the decreased magnitude of TC and LDL-C concentrations ( r = 0.67,0.59, both P＜0.01 ). Conclusion: QYXBC can lower the level of blood lipids and improve significantly EDVR function.     

Study on Effect of Zhixinkang Capsule （脂欣康胶囊）in Treating Unstable Effort Angina and Hyperlipidemia and Its Function in Vascular Endothelium Protection

Objective:To observe the clinical effect and protection of vascular endothelium of Zhixin-kang Capsule (ZXKC) in middle-aged and old people with unstable effort angina and hyperlipidemia. Methods: Sixty-five patients with unstable effort angina were randomly divided into ZXKC group (34 cases) and control group (31 cases). Conventional western medical therapy was given to both groups, with ZXKC group receiving additional ZXKC treatment. Data of 20 healthy persons were taken as normal group. Forty-eight patients with hyperlipidemia were divided into ZXKC group treated with ZXKC (31 cases) and control group treated with Yixintong (17 cases). The changes of clinical symptoms and laboratory indexes in all the patients were observed before and after treatment. Results: In patients with unstable effort angina, the efficacy of treatment of ZXKC, the withdrawal rate of nitroglycerin, the relieving of symptoms, the improvement of the electrocardiogram, the counts of circulating endothelial cells, the content of p     

Effect of Captopril on Antithrombus Function of Endothelium

ＥｆｆｅｃｔｏｆＣａｐｔｏｐｒｉｌｏｎＡｎｔｉｔｈｒｏｍｂｕｓＦｕｎｃｔｉｏｎｏｆＥｎｄｏｔｈｅｌｉｕｍＸＩＯＮＧＹｉ－ｌｉ（熊一力）；ＺＨＡＯＨｕａ－ｙｕｅ（赵华月）（ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉｃｉｎｅ，ＴｏｎｇｊｉＨｏｓｐｉｔａｌ，Ｔｏｎｇ...     

Effects of Erigeron Injection on Vascular Endothelium in Patients

Objective： To observe the levels of serum C-reactive protein （CRP）, endothelin-1 （ET-1）, nitric oxide （NO）, and superoxide dismutase （SOD） in patients after intracoronary stenting （ICS）, and the effects of Erigeron Injection （E1） on them. Methods： Seventy-two patients, who received ICS and had symptoms of chest stuffiness, palpitation and chest pain, were randomly divided into two groups, with 36 patients in the control group treated with Plavix alone for anti-platelet aggregation, and the other 36 patients in the treated group treated with Plavix and El in combination. CRP, ET-1, NO and SOD were determined and compared before and 1, 2 and 3 weeks after treatment. Results： As compared with those in the control group, improvement of symptoms in the treated group was significantly better, with the levels of CRP and ET-1 lower and levels of SOD and NO higher or approaching to normal ranges and significant difference was shown between the two groups （P〈0.01）. Conclusion： El could alleviate uncomfortable feelings such as chest stuffiness in patients after lOS, and improve the function of vascular endothelium.     

Clinical Study of Xiongshao Capsule (芎芍胶囊) in Preventing Restenosis after Coronary Interventional Treatment

Ｓｉｎｃｅｐｅｒｃｕｔａｎｅｏｕｓｔｒａｎｓｌｕｍｉｎａｌｃｏｒｏｎａｒｙａｎｇｉｏｐｌａｓｔｙ（ＰＴＣＡ）ａｎｄ／ｏｒｃｏｒｏｎａｒｙｓｔｅｎｔｉｎｇｃａｎｒｅｃｏｎｓｔｒｕｃｔｃｏｒｏｎａｒｙｂｌｏｏｄｃｉｒｃｕｌａｔｉｏｎｗｉｔｈ ｏｕｔｔｈｏｒａｃ     

Clinical Observation on Effect of Kaixin Capsule （开心胶囊） in Treating 40 Patients with Diabetic Myocardial Ischemia

Objective： To observe the effect of Kaixin Capsule （开心胶囊, KXC） on myocardial ischemia and plasma endothelin （ET） level in patients with diabetic heart disease （DHD）. Methods： The 72 subjects for observation were randomly selected from inpatients whose diagnosis fit to the standard of DHD. The 32 patients allocated in the control group were treated with conventional Western medicine, and the 40 patients in the treated group were treated with conventional Western medicine in combination with KXC, with the therapeutic course for both groups as 60 days. Results： On ECG, the total effective rate and markedly ef- fective rate in the treated group was 85. 0% and 37. 5% respectively, higher than those in the control group＇s 68.7% and 28. 1% respectively, and showing significant difference between the two groups （P〈 0.05）. The level of ET in patients in both groups was significantly higher than normal range, after treatment, but reduced to different extent, and the comparison between them also showed that the difference was significant （P〈0.05）. Conclusion： KXC might, by way of inhibiting and blocking the release of ET, lower its level in plasma so as to improve the myocardial ischemic condition of patients with DHD.     

Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on Abnormal Methylation in Rabbits with Atherosclerosis

Objective: To investigate the effects of Zhizi Chuanxiong Capsule (ZCC, 栀子川芎胶囊) on abnormal DNA methylation in a rabbit model of atherosclerosis (AS). Methods: After 1 week of adaptive feeding, 48 New Zealand white rabbits were randomly divided into 4 groups: a control group (n=12) fed with normal diet for 22 weeks; a model group (n=12) fed with high fat diet for 14 weeks followed by 8 weeks of normal diet feeding; a low-dose ZCC group (n=12) fed with high fat diet and low-dose ZCC for 14 weeks, followed by 8 weeks of normal diet and low-dose drug; a high-dose ZCC group (n=12) fed with high fat diet and high-dose drug for 14 weeks, followed by 8 weeks of normal diet and high-dose drug. After 22 weeks of feeding, blood samples were taken from the rabbit ear vein, and the genomic DNA was extracted for methylation immunoprecipitation sequencing (Medip-seq). The aorta tissues were collected for hematoxylin-eosin (HE) staining. Results: Eight rabbits died during the feeding process. HE staining showed that the size of the lipid deposition on vessel wall and atherosclerotic plaque formation were reduced in both low- and high-dose group. The Medip-seq results showed that there were 146 abnormally methylated genes (including both hypermethylated gene and hypomethylated genes) in the model group, compared with the control group. Gene Ontology (GO) and Pathway analysis showed that these abnormally methylated genes were found to be involved in multiple AS-related functions and pathways, such as protein kinase C activity, cholesterol transport, mitogen-activated protein kinase (MAPK) signaling pathway, peroxisome proliferater-activated receptor signaling pathway, vascular smooth muscle contraction, inflammation and so on. The abnormal methylated genes in AS model group were altered in both low- and high-dose groups: low-dose ZCC could change 72 of the 146 abnormally methylated genes, high-dose ZCC could change 71. Through GO and Pathway analysis, these altered methylated genes were involved in protein kinase C activity, inflammatory pathway, MAPK signaling pathway, vascular endothelial growth factor signaling pathway, etc. Conclusion: ZCC could treat AS through regulating the abnormal hypermethylated and hypomethylated genes in AS rabbit model.     

Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on Abnormal Methylation in Rabbits with Atherosclerosis

Objective: To investigate the effects of Zhizi Chuanxiong Capsule(ZCC, 栀子川芎胶囊) on abnormal DNA methylation in a rabbit model of atherosclerosis(AS). Methods: After 1 week of adaptive feeding, 48 New Zealand white rabbits were randomly divided into 4 groups: a control group(n=12) fed with normal diet for 22 weeks; a model group(n=12) fed with high fat diet for 14 weeks followed by 8 weeks of normal diet feeding; a low-dose ZCC group(n=12) fed with high fat diet and low-dose ZCC for 14 weeks, followed by 8 weeks of normal diet and low-dose drug; a high-dose ZCC group(n=12) fed with high fat diet and high-dose drug for 14 weeks, followed by 8 weeks of normal diet and high-dose drug. After 22 weeks of feeding, blood samples were taken from the rabbit ear vein, and the genomic DNA was extracted for methylation immunoprecipitation sequencing(Medip-seq). The aorta tissues were collected for hematoxylin-eosin(HE) staining. Results: Eight rabbits died during the feeding process. HE staining showed that the size of the lipid deposition on vessel wall and atherosclerotic plaque formation were reduced in both low-and high-dose group. The Medip-seq results showed that there were 146 abnormally methylated genes(including both hypermethylated gene and hypomethylated genes) in the model group, compared with the control group. Gene Ontology(GO) and Pathway analysis showed that these abnormally methylated genes were found to be involved in multiple AS-related functions and pathways, such as protein kinase C activity, cholesterol transport, mitogen-activated protein kinase(MAPK) signaling pathway, peroxisome proliferater-activated receptor signaling pathway, vascular smooth muscle contraction, inflammation and so on. The abnormal methylated genes in AS model group were altered in both low-and high-dose groups: low-dose ZCC could change 72 of the 146 abnormally methylated genes, highdose ZCC could change 71. Through GO and Pathway analysis, these altered methylated genes were involved in protein kinase C activity, inflammatory pathway, MAPK signaling pathway, vascular endothelial growth factor signaling pathway, etc. Conclusion: ZCC could treat AS through regulating the abnormal hypermethylated and hypomethylated genes in AS rabbit model.     

Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF

Objective  

To evaluate the angiogenic effect of the Xiongshao capsule (芎芍胶囊, XSC) in human umbilical vein endothelial cells (HUVEC), and to investigate the possible molecular mechanisms mediating its biological effect.     

Clinical Study of Effect of Yizhi Capsule(益智胶囊)on Senile Vascular Dementia

Ｃｌｉｎｉｃａｌ　Ｓｔｕｄｙ　ｏｆ　Ｅｆｆｅｃｔ　ｏｆ　Ｙｉｚｈｉ　Ｃａｐｓｕｌｅ（益智胶囊）ｏｎ　Ｓｅｎｉｌｅ　Ｖａｓｃｕｌａｒ　ＤｅｍｅｎｔｉａＣＨＥＮＫａｉ（陈揩），ＣＨＥＮＫｅ－ｊｉ（陈可冀），ＺＨＯＵＷｅｎ－ｑｕａｎ（周文泉），ｅｔａｌ（Ｇ...     

Effects of Erigeron Injection on Vascular Endothelium in Patients after Intracoronary Stenting

Objective: To observe the levels of serum C-reactive protein (CRP), endothelin-1 (ET-1), nitric oxide (NO), and superoxide dismutase (SOD) in patients after intracoronary stenting (ICS), and the effects of Erigeron Injection (El) on them. Methods:Seventy-two patients, who received ICS and had symptoms of chest stuffiness, palpitation and chest pain, were randomly divided into two groups, with 36 patients in the control group treated with Plavix alone for anti-platelet aggregation, and the other 36 patients in the treated group treated with Plavix and El in combination. CRP, ET-1, NO and SOD were determined and compared before and 1, 2 and 3 weeks after treatment. Results: As compared with those in the control group, improvement of symptoms in the treated group was significantly better, with the levels of CRP and ET-1 lower and levels of SOD and NO higher or approaching to normal ranges and significant difference was shown between the two groups (P<0.01). Conclusion: El could alleviate uncomfortable feelings such as chest stuffiness in patients after ICS, and improve the function of vascular endothelium.     

Clinical Study on Effect of Yuantong Capsule in Treating Vascular Dementia

Objective: To observe the clinical effect of Yuantong Capsule (YTC) in treating vascular dementia (VD). Methods: Eighty-three patients of VD were randomized on ratio of 2 : 1 into two groups, the 54 patients in the treated group were treated with YTC orally administered, 3 times a     

Clinical Study on Protective Effect of Xinmaitong Capsule on Damage of Vascular Endothelial Cells

Clinical Study on Protective Effect of Xinmaitong Capsule on Damage of Vascular Endothelial Cells@丘瑞香@贺敬波@蓝军     

Effect of Ligustrazine Injection on Vascular Endothelial Cell of The Patients with Arteriosclerosis Obliterans

Effect of Ligustrazine Injection on Vascular Endothelial Cell of The Patients with Arteriosclerosis Obliterans@李尚珠 @黄平平 @刘春华 @王书桂 @钱冠清     

Effect of Xuefu Zhuyu Pill on Patients with Carotid Atherosclerosis by Colour Doppler Ultrasonography

Ｅｆｆｅｃｔ　ｏｆ　Ｘｕｅｆｕ　Ｚｈｕｙｕ　Ｐｉｌｌ　ｏｎ　Ｐａｔｉｅｎｔｓ　ｗｉｔｈ　Ｃａｒｏｔｉｄ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ｂｙ　Ｃｏｌｏｕｒ　Ｄｏｐｐｌｅｒ　ＵｌｔｒａｓｏｎｏｇｒａｐｈｙＬＩＹａｎ－ｍｅｉ；ＣＨＥＮＫｅ－ｊｉ；ＺＨＡＮ...     

Effect of Taizhi''''an Capsule (泰脂安胶囊)Combined with Simvastatin on Hyperlipidemia in Diabetic Patients

Diabetes mellitus ( DM) patients arelikely to develop lipoprotein abnormalitieswith the incidence as high as of 50 %,or e-ven higher in subjects with poor glycemiccontrol(1).Hyperlipidemiais extremely harm-ful on the function of both pancreatic isletsand vascular endothelial cells . Some studiesshowed that the prognosis after acute myo-cardial infarction was worsein diabetics thannon-diabetics(2). The Adult Treat ment PanelⅢ(ATPⅢ) guidelines in the third report byAmerican National Choles…     

Clinical Study on Protective Effect of Xinmaitong Capsule(心脉通胶囊) on Damage of Vascular Endothelial Cells

Ｅｎｄｏｔｈｅｌｉｎ（ＥＴ）ａｎｄｃａｌｃｉｔｏｎｉｎｇｅｎｅｒｅｌａｔｅｄｐｅｐｔｉｄｅ（ＣＧＲＰ）ａｒｅａｐａｉｒｏｆｎｅｗｌｙｆｏｕｎｄｖａｓｃｕｌａｒａｃｔｉｖａｔｉｎｇｍｅｄｉａｗｉｔｈｏｐｐｏｓｉｔｉｖｅａｃｔｉｏｎ．ＥＴｉｓａｖａｓｏｃｏｎｓｔｒｉｃｔｉｖｅｐｅｐｔｉｄｅｐｒｏｄｕｃｅｄｂｙｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｓ（ＶＥＣ）ｗｉｔｈｓｔｒｏｎｇａｎｄｐｒｏｔｒａｃｔｅｄｅｆｆｅｃｔ,ｔｈｅｓｔｒｏｎｇｅｓｔｖａｓｏｃｏｎｓｔｒｉｃｔｏｒｉｎｔｈｅｈｕｍａｎｂ…     

Clinical Study on Effect of Xianlong Capsule (仙龙胶囊) in Treating Senile Vascular Dementia

Ｖａｓｃｕｌａｒｄｅｍｅｎｔｉａ（ＶＤ）ｉｓｏｎｅｏｆｔｈｅｃｈｉｅｆｔｙｐｅｓｏｆｓｅｎｉｌｅｄｅｍｅｎｔｉａ．Ｉｔｉｓａｌｓｏｎａｍｅｄ“ｉｎｖｅｒｓｉｂｌｅｄｅ ｍｅｎｔｉａ”ｄｕｅｔｏｉｔｓｒａｔｈｅｒｃｌｅａｒｐａｔｈｏｇｅｎｅｓｉｓａｓｗｅｌｌ