新型抗氧化剂U74389G对小鼠甘油所致急性肾衰保护作用的初…

观察了新型抗氧化剂Ｕ７４３８９Ｇ对甘油所致急性肾衰小鼠肾功能的保护作用。结果显示，该药能明显减少小鼠血清和肾皮质中氧自由基终末产物丙二醛的含量，减轻肾脏受损程度，治疗组小鼠的血清肌酐显著低于对照组，间质和肾小管的病理损伤也明显轻于对照组，提示该药对甘油所致急性肾衰小鼠的肾功能有显著的保护作用。     

三七总皂甙对兔急性肾衰早期肾功能和结构的保护作用

本文探讨三七总皂甙对甘油所致急性肾功能衰竭（ＡＲＦ）初发期的保护作用，实验兔分为三组；正常对照组（ｎ＝６）ＡＲＦ对照组（ｎ＝１６）和三七组（ｎ＝１０），观察注射甘油后第２４小时兔肾功能和肾组织结构的改变。结果表明，与对照组比较，三七组家活率明显提高，其肾功能指标得到显著改善，肾组织病学改变也减轻。上述结果提示，三七总皂甙甘油所致兔ＡＲＦ早期有明显的保护作用。     

Augiostatin对小鼠肺腺癌LA795的生长抑制作用

为探讨ａｎｇｉｏｓｔａｔｉｎ（血管抑制因子）对小鼠肺腺癌ＬＡ７９５的治疗作用,将４×１０５ＬＡ７９５细胞接种于Ｔ７３９小鼠皮下,细胞接种后第１４ｄ,治疗组给予ａｎｇｉｏｓｔａｔｉｎ腹腔注射,对照组注射等量ＰＢＳ液,共１４ｄ,观察肿瘤大小及动物生存期。结果：治疗组肿瘤直径由２．３５±０．１６ｃｍ缩小至０．９７±０．３４ｃｍ,而对照组瘤体直径由２．２６±０．１２ｃｍ增至４．０２±０．３６ｃｍ,两者之间差异显著（Ｐ＜０．０１）。治疗组平均生存期７６．６ｄ,对照组为３８．４ｄ,统计学分析差异显著（Ｐ＜０．０１）。结论：ａｎｇｉｏｓｔａｔｉｎ可以抑制ＬＡ７９５细胞所致小鼠实验性肿瘤的生长,显著延长动物生存期,可能是治疗恶性肿瘤的一种全新的有效物质。     

肾康灌肠液延缓慢性肾衰进展的临床研究

慢性肾衰（ＣＲＦ）是多种肾脏疾病晚期的综合症候群，临床任何治疗的主要目的均是近期内改善临床症状，延缓病程的进展速度。笔者在严格饮食管理、排除肾功能恶化因素的影响下，以中药肾康灌肠液为主治疗慢性肾衰１６例，观察中医药对慢性肾衰病程进展的影响，获效较为满意。现总结如下。１　材料和方法１．１　病例选择与分组　３０例非透析ＣＲＦ住院病人病因均为慢性肾炎，入院时随机分为两组。对照组１４例，血清肌酐（Ｓｃｒ）６００±２４０μｍｏｌ／Ｌ，采用低蛋白饮食（ＬＰＤ）、对症等治疗；观察组１６例，Ｓｃｒ５９０±２１０…     

中药合剂免疫调节作用的实验研究

研究了自拟中药合剂对小鼠血象及巨噬细胞Ｃ＿３ｂ和Ｆｃ受体活性的影响，以探讨该合剂的免疫调节作用。结果表明，该合剂可使受化疗损伤小鼠ＷＢＣ明显提高，与化疗对照组相比具有显著的差异（Ｐ＜０．０５）；使用该中药合剂的小鼠腹腔巨噬细胞ＹＣ花环形成率（２３．１０±７．０５％）和ＥＡ花环形成率（２５．４７±５．５５％）较化疗对照组均有明显提高（Ｐ＜０．０１）。认为该中药合剂具有升高ＷＢＣ的作用和免疫调节作用     

五灵丸对兔和大小鼠肝损伤保护作用的实验研究

五灵丸对四氯化碳和醋氨酚所致兔与大小鼠血清ＡＬＴ，ＡＳＴ酶升高有明显地降低作用，且能降低胆固醇，甘油三酯与ＭＤＡ；提高肝内ＧＳＨ－ＰＸ为１．７４±０．６０Ｕ／ｍｇ治疗组为３．５６±０．７６Ｕ／ｍｇ（Ｐ＜０．０１），对照组大鼠ＧＳＨ－ＰＸ为１．９５±０．４４Ｕ／ｍｇ；治疗组２．４３±０．３０Ｕ／ｍｇ（Ｐ＜０．０１）。提高大鼠免疫功能，对照组刺激指数为０．９９±０．１９；治疗组为２．２８±１．４８...     

银耳枸杞煎剂抗衰老作用的实验研究

为研究银耳枸杞煎剂的抗衰老作用，用化学比色法测定了给药前后小鼠血清中超氧化物歧化酶（ＳＯＤ）活力，丙二醛（ＭＤＡ）含量，测定了给药前后小鼠耐缺氧存活时间，游泳存活时间。给药前ＳＯＤ活力为８１．８３±１６．１６ＶＵ／ｍｌ，ＭＤＡ含量为４．８２±１．８４ｎＭ／ｍｌ，耐缺氧存活时间为６．４４±１．０２分，游泳存活时间为１．９１±０．６６分；给药后ＳＯＤ活力为１０８．８５±２１５２，ＭＤＡ含量为３．０５±１．２１，耐缺氧存活时间为７．６８±１．０９游泳存活时间为２．６６±０．７２。各项指标给药前后行ｔ检验，均存在显著差别。     

硫普罗宁对实验性肝损伤的保护作用

目的：观察硫普罗宁（ＭＰＧ）对小鼠实验性肝损伤的保护作用。方法：采用Ｄ－氨基半乳糖（ＤＡＧ）和乙醇（Ｅｔｈ）所致动物肝损伤的方法来研究ＭＰＧ的保肝作用。结果：ＭＰＧ灌胃剂量为１２５ｍｇ／ｋｇ·ｄ及２５０ｍｇ／ｋｇ·ｄ，对ＤＡＧ和Ｅｔｈ所致的小鼠急性肝损伤有明显的保护作用。表现在ＭＰＧ能使血清中ＡＬＴ、ＡＳＴ明显降低，甘油三酯蓄积量减少，肝细胞变性坏死明显减轻。结论：ＭＰＧ具有防治由ＤＡＧ和Ｅｔｈ所致的肝损伤作用。     

白血病环腺苷一磷酸水平,腺苷酸环化酶和磷酸二酯酶比活化的比较

采用同位素测定法分别对急性和慢性白血病患者血浆环腺苷一磷酸（ｃＡＭＰ）、腺苷酸环化酶（ＡＣ）和环腺苷酸磷酸二酯酶（ｃＡＰＤＥ）三项指标进行了检测，并与非白血病患者和正常献血员进行比较，发现白血病组的ｃＡＭＰ水平分别为１０．２３±１．６４和１２．５７±１．３８ｐｍｏｌ／ｍｌ，较正常对照组２９．７８±２．６６ｐｍｏｌ／ｍ显著降低（Ｐ〈０．０１），ＡＣ比活力分别为０．３２±０．０２和０．４４±０．０６Ｕ     

荷人肺腺癌裸小鼠血清癌胚抗原含量与瘤重的关系

用放射免疫双抗体法测定了荷第１１代人肺腺癌移植瘤裸小鼠的血清癌胚抗原（ＣＥＡ）含量。结果表明，荷瘤裸小鼠血清ＣＥＡ含量比对照组显著增加（分别为７７．３３±３５．１１ｎｇ／ｍｌ、９．６８±４．７４ｎｇ／ｍｌ），在瘤重量小于１ｇ时，ＣＥＡ含量与瘤重是非常显著的正相关（ｒ＝０．９０，Ｐ＜０．０１），在瘤重大于１ｇ时，血清ＣＥＡ含量增加缓慢。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.