联合治疗Ⅲ型前列腺炎的疗效观察

目的探讨Ⅲ型前列腺炎更有效的治疗方法。方法选择80例临床诊断为慢性前列腺炎／慢性盆腔疼痛综合征患者,随机分为2组：联合组为体外电场热疗联合坦索罗辛及抗生素治疗,对照组为单用药物治疗组,观察治疗前后变化并对比疗效分析。结果治疗后2组患者均有明显疗效,联合组总有效率92．5％（37／40）,对照组总有效率82．5％（33／40）,联合组与对照组比较差异有显著性意义（P〈0．05）。结论体外电场热疗联合坦索罗辛及抗生素治疗疗效优于单用药物治疗组,值得临床进一步推广应用。     

穴位注射配合耳穴贴压治疗妇女更年期综合征107例观察

目的:观察穴位注射配合耳穴贴压治疗妇女更年期综合征的疗效。方法:选择207例更年期综合征患者分为3组,对照组1(50例)采用穴位注射疗法,对照组2(50例)采用耳穴贴压治疗,观察组采用穴位注射配合耳穴贴压治疗,观察并比较3组疗效。结果:观察组总有效率为96.3%,优于对照组1(54.0%)和对照组2(78.0%)。结论:穴位注射配合耳穴贴压治疗妇女更年期综合征疗效好、简便、经济、患者易接受且无副作用,值得临床推广应用。     

耳穴贴压结合心理护理对患者失眠状态的影响

目的:观察耳穴贴压结合心理护理治疗患者失眠的临床疗效。方法:选择失眠患者172例,随机分为治疗组和对照组,每组86例,治疗组予耳穴贴压治疗和心理护理;对照组予艾司唑仑或安定治疗和常规护理;治疗2个月后,比较两组患者的临床疗效。结果:治疗组总有效率为97.67%,对照组总有效率为74.42%,两组比较差异有统计学意义(P<0.05)。结论:耳穴贴压结合心理护理对患者失眠有效。     

复方玄驹胶囊联合坦索罗辛治疗慢性非细菌性前列腺炎的临床观察

目的观察复方玄驹胶囊联合盐酸坦索罗辛治疗慢性非细菌性前列腺炎的临床疗效。方法将162例慢性非细菌性前列腺炎患者随机分为观察组（81例）和对照组（81例）。对照组给予前列康片口服,治疗组给予复方玄驹胶囊和盐酸坦索罗辛口服。两组均以4周为1个疗程,治疗前、后分别采用美国国立卫生研究院慢性前列腺炎症状指数评分（NIH-CPSI）对症状进行评测和比较。结果两组患者经过治疗后,在疼痛或不适症状评分、排尿症状评分、生活质量评分及总分4项上均有明显改善（P〈0.05或P〈0.01）,观察组疗效（87.7%）较对照组（64.2%）显著。结论复方玄驹胶囊联合坦索罗辛治疗慢性非细菌性前列腺炎安全有效。     

耳穴贴压疗法对妊娠剧吐患者胃动素水平的影响

目的观察耳穴贴压疗法治疗妊娠剧吐的临床疗效,并研究其对患者胃动素(MOT)水平的影响。方法将2012年本院收治的64例妊娠剧吐患者随机分为耳穴贴压组及常规治疗组,并设30例正常早孕为对照组。耳穴贴压组采用耳穴贴压疗法联合常规治疗,常规治疗组仅给予常规西医支持治疗。观察比较2种治疗方法的临床疗效及治疗前后MOT水平的变化。结果耳穴贴压组治愈率明显高于常规治疗组,差异具有统计学意义(P0.05),但2组总有效率比较,差异无统计学意义。治疗前,耳穴贴压组、常规治疗组的血清MOT水平均低于对照组(P0.05),而耳穴贴压组与常规治疗组比较差异无统计学意义(P0.05);治疗后,耳穴贴压组及常规治疗组MOT水平均升高(P0.05),耳穴贴压组升高更显著,与常规治疗组相比差异有统计学意义(P0.05)。结论妊娠剧吐的发生可能与患者MOT水平下降有关。耳穴贴压联合西医支持治疗妊娠剧吐效果显著,这可能与其能升高患者MOT水平,加强胃肠运动,改善进食欲望有关。     

耳穴贴压治疗溃疡性结肠炎精神心理症状疗效观察

目的：耳穴贴压治疗溃疡性结肠炎精神心理症状的疗效观察。方法：将50例病例随机分为两组,分别采用常规治疗护理措施、耳穴贴压配合常规治疗护理措施的方法。结果 ：经治疗1个疗程后,常规治疗组（对照组）总有效率为68%;耳穴贴压组（干预组）总有效率为84%。结论：干扰组的疗效优于对照组,且具有统计学意义（P〈0.05）。     

督脉灸联合耳穴贴压治疗心虚型失眠的临床观察

目的 观察督脉灸联合耳穴贴压治疗失眠的临床疗效。方法 符合纳入标准的180例心虚型失眠患者,采用SAS程序产生随机分配方案分为观察组和对照组各90例,观察组采用督脉灸联合耳穴贴压,对照组单纯用耳穴贴压疗法,疗效观察采用匹兹堡睡眠质量指数（Pittsbrugh sleep quality index,PSQI）,对比观察2组治疗后的疗效及睡眠效率。结果 观察组总有效率为79%,对照组总有效率为47%,观察组优于治疗组（P<0.01,Z=-4.09）;在睡眠效率这一指标上,观察组在治疗前后的比较有统计学意义（P<0.01,t=-1.17）,而对照组治疗前后的比较无统计学差异。结论 督脉灸联合耳穴贴压治疗失眠简单有效,其可明显改善失眠患者的睡眠效率。     

前列腺内药物注射治疗慢性前列腺炎的疗效观察和护理要点

目的观察前列腺内药物注射治疗慢性前列腺炎的疗效。方法将92例慢性前列腺炎患者随机分为两组,观察组48例前列腺内注射抗生素和中药,并相应给与针对性的健康教育和心理护理;对照组44例口服抗前列腺炎药物,临床治疗4周,观察两组的疗效。结果观察组治疗有效率为87.5%,与对照组相比,合用中药注射治疗慢性前列腺炎比口服抗前列腺炎药物更为有效（P〈0.05）。结论前列腺内注射抗生素和中药是治疗慢性前列腺炎的有效方法,治疗同时给予相应针对性的健康教育和心理护理措施是有效的辅助手段。     

坦索罗辛联合前列欣治疗慢性前列腺炎临床疗效研究

目的探讨坦索罗辛联合前列欣治疗慢性前列腺炎的临床疗效,为治疗该病探求更优化的治疗方法。方法纳入95例慢性前列腺炎患者作为研究对象,将其随机分为对照组和治疗组,采用单中心、随机、对照研究。对照组给予常规药物及坦索罗辛治疗;治疗组在常规治疗的基础上,加用前列欣治疗,总治疗时间28 d。观察两组患者治疗前、后临床疗效。结果治疗后,治疗组总有效率为96.3%,显著优于对照组(74.4%),治疗过程中未见严重不良反应。结论在基础治疗上加坦索罗辛和前列欣治疗慢性前列腺炎疗效显著,值得临床推广运用。     

中药穴位贴敷辅助治疗慢性阻塞性肺病的临床疗效观察

目的：探讨中药穴位贴敷辅助治疗COPD的临床疗效。方法：120例COPD患者随机分为对照组和治疗组各60例,对照组采用常规治疗给予祛痰剂、抗生素、支气管扩张剂等,治疗组在对照组基础上,增加中药穴位贴敷治疗,比较两组患者的临床疗效和肺功能改善情况。结果：治疗组总有效例数为55例,总有效率91.7%;对照组总有效例数为46例,总有效率为76.7%;治疗组总有效率显著高于对照组（X^2=5.07,P〈0.05）。而治疗组FEV1、FEV1/FVC和FEV1/PR等肺功能指标的改善程度均显著大于对照组（P〈0.05）。结论：中药穴位贴敷辅助治疗慢性阻塞性肺病（COPD）具有良好的临床效果,可以更好的改善患者肺功能,值得临床推广应用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.