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1.
p53基因是一种肿瘤抑制基因,当其缺失或突变时则成为许多肿瘤的始因之一,而肿瘤的转移能力与CD44的异常表达明显相关,研究表明,p53蛋白和CD44变异体在喉癌组织中的表达有助于了解喉癌的发生、发展、分化程度及预后,对喉癌的治疗提供了生物学依据。 相似文献
2.
p53,c-myc,nm23癌基因蛋白在喉复发癌的表达及其意义 总被引:6,自引:1,他引:6
目的:研究p53,c-myc,nm23癌基因蛋白的表达与喉癌复发的关系,对预后作出估计。方法:回顾分析喉癌手术标本574例,术后随访3年,确诊术后复发56例。正常喉对照标本39例。标本制成组织芯片,采用免疫组化SABC法对芯片标本进行染色。结果:p53,nm23、c-myc在喉癌组织中有表达,其阳性率各有不同,其中c-myc在喉癌复发组的表达明显增强,与未复发组的表达差异有极显著性意义(P<0.01);而nm23在喉癌复发组的表达明显减弱,与未复发组的表达差异有极显著性意义(P<0.01)。p53在喉癌复发组与复发组的表达差异无显著性意义(P>0.05)。c-myc的表达与喉癌复呈正相关,nm23癌基因蛋白的表达与喉癌复发呈负相关。结论:c-myc,nm23癌基因蛋白的表达可作为预测喉癌术后复发的指标。 相似文献
3.
喉癌中环氧化酶-2和p53的表达及相关性研究 总被引:2,自引:2,他引:2
目的 :了解喉癌组织中环氧化酶 2 (Cox 2 )及p5 3的表达 ,探讨Cox 2在喉癌发生发展中的作用及与p5 3之间的关系。 方法 :采用免疫组织化学方法检测 6 3例喉癌组织中Cox 2与 p5 3的表达情况。 结果 :6 3例喉癌标本中Cox 2阳性表达率为 5 8.7% (37/6 3) ,患者的不同临床分期的表达差异有统计学意义 (P <0 .0 5 )。p5 3的阳性表达率为 6 3.5 % (4 0 /6 3) ,不同组织病理学分级的 p5 3阳性表达的差异有统计学意义 (P <0 .0 5 ) ;Cox 2与p5 3之间存在等级相关性。结论 :Cox 2能促进喉癌的生长、增殖与浸润 ,应用Cox 2抑制剂可望对喉癌的发展起到抑制作用 相似文献
4.
目的:了解喉乳头状瘤组织内HPV16/18的感染与抑癌基因p53变异的关系,以及HPV感染在喉乳头状瘤发病中的作用。方法:采用PCR和免疫组化技术,检测35例喉乳头状瘤组织中HPV16/18DNA及p53蛋白的表达。结果:24例组织中检出HPV16/18DNA(68.6%);19例p53蛋白呈过度表达(54.3%);在12例中同时检出HPV16/18DNA和p53蛋白过度表达(34.3%)。结论: 相似文献
5.
本研究采用免疫组化法 ,观察 p2 1和 p53癌基因产物在不同部位、不同临床分期和不同细胞分化及有随访资料的喉鳞癌 ( LSC)中的表达情况 ,以探讨 LSC发生过程中癌基因的表达规律及与预后的关系。1 材料和方法1 .1 材料来源全部组织选自本院病理科 1 987~ 1 996年有完整随访 相似文献
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喉癌前病变p53过度表达的研究 总被引:5,自引:0,他引:5
采用标记链霉菌素-生物素免疫酶技术结合微波炉处理方法,对15例成人型喉头状瘤,10例喉部不典型增生,2例喉原位癌,10例声带息肉以及6例正常喉的组织标本进行了检测,结果发现:在73.3%喉乳头状瘤,40%喉不典型增生和50%喉原位癌中检测到p53蛋白过度表达,而10例声带息肉和6例中正常组织无一例出现p53的过度表达。 相似文献
7.
目的 :探讨鼻咽癌 (NPC)组织中主要热应激蛋白 (HSPs)和 p5 3表达水平及其临床意义。 方法 :用Westernblot方法检测 38例NPC组织和 17例鼻咽慢性炎症组织中HSPs和 p5 3的表达。 结果 :HSP 2 7和 p5 3在NPC组织中表达升高 ,与鼻咽炎症组织比较 ,差异有显著性意义 (P <0 .0 5 ) ;但在NPC的组织分型和临床分期之间 ,差异无显著性意义 (P >0 .0 5 )。HSP 90和HSP 70在NPC组织和慢性炎症组织间差异无显著性意义 (P>0 .0 5 )。结论 :HSPs和 p5 3在NPC组织中存在一定程度的异常表达 ,提示其在NPC的发病机制中可能发挥一定作用 相似文献
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目的 探讨p16蛋白表达在甲状腺癌发生、发展中的作用及其临床意义。方法采用免疫组织化学SP法检测p16蛋白在甲状腺癌组织中的表达或缺失。结果p16蛋白在甲状腺乳头癌和甲状腺滤泡癌组织中的表达率明显高于甲状腺髓样癌和未分化甲状腺癌组织的表达率(P<0.05)。Ⅰ期p16蛋白的表达率明显高于Ⅲ、Ⅳ期(P<0.05)。无淋巴结转移组p16蛋白的表达率(92.6%)高于有淋巴结转移组(64.7%)(P<0.05)。高分化组p16蛋白的表达率(90.3%)高于低分化组(61.5%)(P<0.05)。结论p16蛋白参与了甲状腺癌的发生和发展,与甲状腺癌的临床分期、分化程度、淋巴结转移有关,可作为临床判定预后的参考指标之一。 相似文献
10.
Pin Dong Xiao-yan Li Guang-zhong Lu Zheng-hua Zhu Zi-wei Yu Bin Jin Jiang-cai Zhu 《中华耳鼻咽喉科杂志》2004,39(11):683-686
OBJECTIVE: To evaluate the predictive role of fibronectin, p81 (Ezrin protein) and p53 gene in primary laryngeal carcinoma, it's relationship with epidemiology(smoking), histological grading, surgical treatment, TNM stage and prognosis were studied by the tissuechip technology. METHODS: The expression of fibronectin, p53 gene and p81 (Ezrin protein) on a series of 85 primary laryngeal carcinoma patients treated in our hospital between 1992 and 2000 was studied with tissuechip technology. The correlation of each score according to the intensity and percentage of labeled cells or intercellular substance with relevant clinical dada was statistically analyzed. RESULTS: Some cases were lost or boasted no tumor tissue in our tissuechip. Among the 70 cases available, 45.71% (32/70) of the specimens' basal membrane and extracellular matrix were strongly stained with fibronectin; there is statistical significance (P < 0.05) between primary tumor grading groups. Ezrin protein expressing rate is 87.3%, and the average percentage of its labeled cells is 53.68% (ranging from 0% to 100%, median is 58. 69%). There were significant difference between tumor grading groups, clinical early and late stages and 3-year survival rates (P < 0.05) after chi-square test. But no relation with smoking, gender, age and histological classes (P > 0.05). The average percentage of p53 positive cells is 21.6% (ranging from 0% to 90.3%, median is 5.85%) and 46.8% showed positive stains in our research. There was no statistical prominence in p53 protein demonstration between TNM stages, lymph node metastasis, 3-year survival rate, smoking, gender, age and histological classes (P > 0.05). CONCLUSIONS: The tissue microarray technique spent shorter time and less expense, and showed higher consistency in our essays. And the present study suggests fibronectin and p81 (Ezrin protein) could be the clinical discriminators in laryngeal carcinoma. 相似文献
11.
J.‐P. Jeannon J. Soames J. Lunec S. Awwad V. Ashton J. A. Wilson 《Clinical otolaryngology》2000,25(1):23-27
The prognostic value of the expression of the cyclin‐dependent kinase inhibitor p21 and the p53 tumour suppressor gene was examined using immunohistochemistry in 60 patients with laryngeal cancer. Multivariate analysis using Cox's proportional hazard method, showed that p21 expression (P = 0.02) and advanced T stage (P = 0.003) significantly predicted survival. It was concluded that p21 expression may be a useful prognostic indicator in laryngeal cancer. 相似文献
12.
Yi Li Bo Li Bo Xu Bo Han Hui Xia Qian-Ming Chen Long-Jiang Li 《International journal of oral science》2015,7(3):161-168
The present study evaluated the expression of key molecules and the status of DNA in both oral squamous cell carcinoma (OSCC) and adjacent tissues to establish a molecular surgical boundary and provide a cancer progression model. Biopsy samples from 50 OSCC patients were divided into T (cancer), P1 (0–0.5 cm), P2 (0.5–1 cm), P3 (1–1.5 cm) and P4 (1.5–2 cm) groups based on the distances from the visible boundary of the primary focus. Twenty samples of normal mucosa were used as controls. We used immunohistochemical staining and flow cytometry to evaluate p53, p21
CIP1/WAF1
, eIF4E and Ki-67 expression and to determine DNA status, respectively. Sub-mucosal invasion was present in the P1 and P2 groups as determined by haematoxylin and eosin staining. Mutant p53 expression decreased gradually from cancerous to normal mucosae, whereas p21
CIP1/WAF1
expression displayed an opposite trend. eIF4E expression decreased from cancerous to normal mucosae. Ki-67 expression, the heteroploidy ratio, S-phase fraction and proliferative index decreased gradually with the distance from the tumour centre. Based on these results, we suggest that the resection boundary in OSCC surgery should be beyond 2 cm from the tumour. Additionally, the adjacent tissues of the primary focus could be used as a model for assessing cancer progression. 相似文献
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目的探讨p16基因及增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)在喉鳞状细胞癌中的表达及其临床意义。方法应用免疫组织化学技术SP法检测抑癌基因p16蛋白及PCNA在40例喉鳞状细胞癌中的表达,并与癌旁组织和正常喉组织对比,结合喉鳞状细胞癌的临床病理特征进行分析。结果在喉鳞状细胞癌中p16阳性率(50.0%)明显低于癌旁组织(76.0%)和正常组织(100.0%,P<0.05);Ⅰ、Ⅱ期喉癌中p16阳性率明显高于Ⅲ、Ⅳ期。喉鳞状细胞癌中PCNA阳性率为55.0%,明显高于癌旁组织(32.0%)和正常组织(10.0%,P<0.05);Ⅰ、Ⅱ期喉鳞状细胞癌中PCNA表达明显低于Ⅲ、Ⅳ期(P<0.05)。p16阳性与阴性患者生存期≥5年的比率分别为66.7%和33.3%,P<0.05;PCNA阳性与阴性患者生存期≥5年的比率分别为37.5%和62.5%,P<0.05。结论p16和PCNA分别是喉鳞状细胞癌进展及预后判断的独立指标;p16和PCNA联合检测对喉鳞状细胞癌的预后评估可能更有价值。 相似文献
15.
Prognostic value of the expression of p53 and bcl-2 in patients with laryngeal carcinoma 总被引:3,自引:0,他引:3
J. Klatka 《European archives of oto-rhino-laryngology》2001,258(10):537-541
Previous studies have yielded inconsistent results on the prognostic significance of p53 and bcl-2 in head and neck cancer.
The aim of this study was the evaluation of p53 and bcl-2 protein expression in laryngeal squamous cell carcinoma and to clarify
the relationship between them. All patients with laryngeal carcinoma were treated during the period 1991–1993. In the present
study, p53 and bcl-2 expression in paraffin sections from 50 cases of laryngeal squamous cell carcinoma were analysed and
correlated with routine clinico-pathological parameters. The expressions of p53 and bcl-2 were examined by immunohistochemical
staining. Immunoreactivity for p53 was observed in 45 (90%) of carcinomas and bcl-2 immunoreactivity in 7 (14%). No significant
correlation between the p53 or bcl-2 expression and patients’ T- or N-stage, histological grading, or overall survival was
found.
Received: 2 May 2001 / Accepted: 7 June 2001 相似文献
16.
目的 观察喉癌中maspin和p53蛋白的表达,探讨maspin和p53在喉癌发生、发展中的作用。方法 采用免疫组化法检测78例喉鳞癌组织中maspin和p53蛋白。结果 在喉癌中maspin阳性表达率为55.1%(43/78),maspin表达下降与病理分化程度低、淋巴结转移有关。p53阳性表达率为58.9%(46/78)。在maspin阳性表达的43例喉癌中,p53阳性表达率为79.1%,maspin阴性表达的喉癌中,p53阳性表达率为34.3%,差异有统计学意义(P<0.05)。Spearman等级相关分析显示,maspin在喉癌组织中的表达与p53呈负相关性,有统计学意义(rs=-0.381, P=0.006)。结论 maspin在喉癌的发生发展中起一定抑制作用。喉癌中maspin表达与p53表达呈负相关,maspin可能是p53的效应基因。 相似文献
17.
喉癌中p53蛋白与VEGF表达的相关性研究 总被引:7,自引:3,他引:4
目的检测、比较分析喉癌中p53蛋白与VEGF的表达及平均瘤内血管密度的相关性.方法采用S-P免疫组织化学的方法检测62例喉癌术后标本中p53蛋白和VEGF的表达,并计算其平均瘤内血管密度;同时采用SPSS统计软件分析p53蛋白与VEGF的表达及瘤内血管密度的相关性.结果 62例喉癌中,33例p53蛋白阳性,38例VEGF阳性,平均瘤内血管密度为(34.58±11.23)mv;p53蛋白阳性的33例中,25例VEGF表达阳性,平均瘤内血管密度为(39.65±10.66)mv;29例p53蛋白阴性的病例中,13例VEGF表达阳性,平均瘤内血管密度为(30.45±11.76)mv;统计学处理发现p53蛋白阳性的病例中,VEGF表达的阳性率高于p53阴性的病例,平均瘤内血管密度高于p53阴性的病例.结论喉癌中VEGF的表达、平均瘤内血管密度与p53蛋白的表达呈正相关,mut-p53基因可能具有促进喉癌中VEGF表达、促进喉癌中微血管形成,进而促进喉癌细胞的侵袭与转移的作用. 相似文献