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1.
Zhang Xiping Wu Dijiong Liang Jianfeng Cheng Qihui Ye Jing Jia Penghui Yuan Meijuan Zhou Ninni 《Inflammation》2009,32(4):218-232
To observe the protective effects of Salvia miltiorrhizae injection on multiple organs of rats with SAP or OJ. Two hundred
eighty-eight rats were used for SAP-associated experiments and OJ-associated experiments. The rats were randomly divided into
sham-operated, model control and treated group. According to the different time points after operation, the SAP rats in each
group were subdivided into 3, 6 and 12 h groups while the OJ rats were divided into 7, 14, 21 and 28 days groups. The mortality
rates, pathological changes and ICAM-1 (only in lung), TLR4 (only in liver), Bax and NF-κB proteins expression in multiple
organs (liver, kidney, lung, intestinal mucosa, spleen, thymus and lymph nodes) were observed, respectively. The mortality
rates of treated groups decreased in both SAP and OJ experiments. Compared to model control group, the pathological changes
can be seen in treated groups including; (1) the pathological changes of multiple organs in SAP and OJ experiments were improved,
the pathological severity scores of kidney (at 6 h), intestinal mucosa (at 12 h), spleen (at 6 and 12 h), thymus (at 3 and
6 h) and lymph nodes (at 3 and 6 h) in SAP experiment and of liver (on 21 and 28 days), lung (21 days), intestinal mucosa
(on 21 and 28 days) and spleen (on 21 and 28 days) in OJ experiment significantly declined (P < 0.05 or P < 0.01); (2) the positive rate of Bax protein in pancreas (at 3 h) and liver (on 6 and 12 h) in SAP experiment and in liver
(on 28 days), kidney (on 21 days), and thymus (on 28 days) in OJ experiment were significantly lower (P < 0.05 or P < 0.01); (3) the positive rate of NF-κB in liver (at 12 h) and kidney (at 6 h) in SAP experiment and in liver (on 21 days),
kidney (on 28 days), intestinal mucosa (on 28 days) and thymus (on 14 days) in OJ experiment significantly decreased (P < 0.05). (4) the positive rate of ICAM-1 in lungs (at 12 h) in SAP experiment was significantly less (P < 0.05). Salvia miltiorrhizae injection can protect multiple organs of SAP or OJ rats and inhibit the expression of ICAM-1,
TLR4, NF-κB and regulate Bax proteins. Salvia miltiorrhizae may relieve the inflammation response and enhance the immunity
function of SAP and OJ rats.
Supported by technological foundation project of Traditional Chinese Medicine Science of Zhejiang province (NO.2003C130; NO.2004C142),
foundation project for medical science and technology of Zhejiang province (No.2003B134), grave foundation project for technological
and development of Hangzhou (NO.2003123B19), intensive foundation project for technology of Hangzhou (NO.2004Z006), foundation
project for medical science and technology of Hangzhou (No.2003A004) and foundation project for technology of Hangzhou (No.2005224)
Note: We claimed that this paper was original and would not have any financial interest in a company or its competitor, and
that all authors meet criteria for authorship. We abided the ethics in this animal experiment study. The ethics committee
approval of our hospital was secured for the animal study reported, and all rats have not been abused and executive mercy
killing when the observing time in this study was over. 相似文献
2.
Zhang Xiping Fu Jun Wu Chengjun Ma Meili Yan Ping Ye Jing Yang Qijun Zhu Feibo Ying Rongcao 《Inflammation》2009,32(5):287-295
To investigate the effect of apoptosis about Salvia miltiorrhizae injection on the lungs of SAP and OJ rats. Total 288 rats
were used for SAP-associated experiments and OJ-associated experiments, respectively. The rats were randomly divided into
sham-operated, model control and treated group. According to the difference of time points after operation, the SAP rats in
each group were subdivided into 3, 6 and 12 h groups while the OJ rats were divided into 7, 14, 21 and 28 days groups. The
pathological changes, expression levels of Bax protein and apoptotic indexes in the lungs of SAP or OJ rats were observed,
and the mortality rates of SAP or OJ rats were recorded, respectively. The numbers of dead SAP and OJ rats in treated groups
declined. The pathological changes in the lungs of SAP or OJ rats in treated groups were relieved to varying degrees. There
was no marked difference in pathological severity scores and the positive staining intensity of Bax protein between treated
groups and model control groups (all P > 0.05). Salvia miltiorrhizae has some protective effect on the lungs of rats with SAP or OJ which may be related apoptosis
although our results can not find significant difference between treated groups and model control groups. 相似文献
3.
Zhang Ruiping Zhang Xiping Zhang Jie Wu Junsheng Ye Qian Xu Rujun Ye Jing Fang Xin Jin Lu He Jiao Yuan Wenqin 《Inflammation》2009,32(2):109-119
To study the efficacy and mechanism of Salvia miltiorrhizae injection in the treatment of severe acute pancreatitis. SAP rat
models were prepared and randomly divided into model control group and treated group. The sham-operated group was also set.
At 3, 6 and 12 h after operation, the mortality rate, ascitic volumes, pathological changes in the pancreas, contents of amylase
and endotoxin in plasma as well as IL-6, IL-18, ET-1 and NO in serum, the staining intensity of Bax and NF-κB p56 proteins,
and the changes in apoptosis index of pancreatic cells in rats in each group were observed. The pathological severity scores
(at 3, 6 and 12 h after operation), contents of plasma endotoxin (at 6 and 12 h after operation) and serum IL-6 (at 6 and
12 h after operation) were significantly lower than those in model control group (P < 0.05, P < 0.01 and P < 0.01, respectively); the staining intensity and the product of the staining intensity and positive staining rate of Bax
protein in the pancreas were significanly higher than those in model control group (P < 0.01). Salvia miltiorrhizae is able to reduce the contents of plasma endotoxin and serum IL-6, promote the expression of
Bax protein in pancreas, improve the pathological changes in the pancreas, and decrease the mortality rate of rats, thereby
showing therapeutic effect on rats with SAP.
Supported by technological foundation project of Traditional Chinese Medicine Science of Zhejiang province (no. 2003C130;
no. 2004C142), foundation project for medical science and technology of Zhejiang province (no. 2003B134), grave foundation
project for technological and development of Hangzhou (no. 2003123B19), intensive foundation project for technology of Hangzhou
(no. 2004Z006), foundation project for medical science and technology of Hangzhou (no. 2003A004) and foundation project for
technology of Hangzhou (no. 2005224)
We claimed that this paper was original and would not have any financial interest in a company or its competitor, and that
all authors meet standard for authorship. We abided the ethics in this animal experiment study. The ethics committee approval
of our hospital was secured for the animal study reported, and all rats have not been abused and executive mercy killing when
the observing time in this study was over. 相似文献
4.
5.
Zhang Xiping Pan Yan Huang Xinmei Feng Guanghua Ma Meili Ni Jie Zhang Fangjie 《Inflammation》2010,33(4):259-266
To observe the protecting effects and mechanisms of Dexamethasone and Salviae miltiorrhizae on intestinal mucosa and immune
organs (spleen, thymus and lymph node) in rats with severe acute pancreatitis (SAP). The rats were randomly divided into sham-operated,
model control, Dexamethasone treated group and Salviae miltiorrhizae treated group. At 3, 6 and 12 h after operation, the
mortality rate, pathological changes of intestinal mucosa and immune organs as well as the contents of serum PAF, IL-1β and
sIL-2R were observed, respectively. The mortality rate and the contents of PAF (at 3 and 6 h), IL-1β (at all time points)
and sIL-2R (at 3 and 6 h) as well as the pathological scores of thymus (at all time points) and spleen (at 3 h) in Dexamethasone
treated group were significantly lower than those in model contrlo groups (P < 0.05). The contents of PAF (at 3 and 12 h), IL-1β (at 6 and 12 h) and sIL-2R (at 3 and 6 h) as well as the pathological
scores of thymus (at all time points) and spleen (at 3 and 12 h) in Salviae miltiorrhizae treated group were markedly lower
than those in model contrlo groups (P < 0.05). Since both Dexamethasone and Salvia miltiorrhizae can reduce the contents of serum PAF, sIL-2R and IL-1β, mitigate
the pathological changes in the small intestine, spleen and thymus and reduce the mortality rate of SAP rats, they show good
therapeutic effects on SAP rats. 相似文献
6.
Influence of Baicalin and Octreotide on NF-κB and P-Selectin Expression in Liver and Kidney of Rats with Severe Acute Pancreatitis 总被引:1,自引:0,他引:1
To observe the influence of Baicalin and Octreotide on liver and kidney of rats with severe acute pancreatitis (SAP) and discuss
the related mechanism. SAP rats were randomly divided into model control, Baicalin treated and Octreotide treated group (n = 45). The same number of normal rats were included in sham-operated group (n = 45). In all groups, the mortality rate, pathological changes as well as expression levels of NF-κB p65 and P-selectin protein
in liver and kidney were observed at 3, 6 and 12 h after operation. The survival rate of treated group was 100% at 12 h significantly
higher than that of model control group (P < 0.05). The pathological changes of liver and kidney in treated groups were alleviated to different degrees, the NF-κB protein
expression levels and pathological severity scores in liver and kidney of treated groups were significantly lower than those
of model control group (P < 0.05 or P < 0.001). The hepatic P-selectin protein expression level in Baicalin treated group was significantly lower than that of
model control group at 3 h (P < 0.01), and renal P-selectin expression level in Baicalin treated group at 3 and 6 h were significantly lower than those
of model control group and Octreotide treated group (P < 0.01). (1) Early treatment with Baicalin or Octreotide have obvious protecting effects on liver and kidney injuries in
SAP with their mechanisms associated to inhibiting NF-κB and P-selectin expression of liver and kidney. (2) Comparing the
pharmacologic effects of Octreotide and Baicalin, we believe Baicalin as a new drug with its protecting effects on liver and
kidney of SAP rats similar to Octreotide is worth further studying. (3) The advantages of tissue microarrays in pathological
examination include time and energy saving and highly efficient. But the restriction of small diameter weakens the representation
of tissues to various extents, which may lead to the deviation of analysis.
Supported by Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province, NO. 2003C130 and
NO. 2004C142; Foundation Project for Medical Science and Technology of Zhejiang province, NO. 2003B134; Grave Foundation Project
for Technological and Development of Hangzhou, NO. 2003123B19; Intensive Foundation Project for Technology of Hangzhou, NO.
2004Z006; Foundation Project for Medical Science and Technology of Hangzhou, NO.2003A004; and Foundation Project for Technology
of Hangzhou, NO. 2005224
Note: We claimed that this paper was original and would not have any financial interest in a company or its competitor, and
that all authors meet criteria for authorship. We abided the ethics in this animal experiment study. The ethics committee
approval of our hospital was secured for the animal study reported, and all rats have not been abused and executive mercifully
killing when the observing time in this study was over. 相似文献
7.
The aim of this study was to observe scutellarin parenteral solution’s therapeutic effects and mechanisms in rats with severe
acute pancreatitis (SAP). We divided SD rats into four groups randomly: (1) sham-operated group, (2) model control group,
(3) scutellarin-treated group, and (4) Salvia miltiorrhiza-treated group. All of those rats in the abovementioned groups are randomly subdivided into 6 and 12 h subgroups, respectively,
according to the postoperative time. Rats have been mercifully killed at different time after operation, and then detected
their serum amylase, contents of ALT, AST, BUN, and Cr and observed the pathologic changes of multiple organs (pancreas, liver,
kidneys, and lungs). We found that the survival rates have no marked differences (P < 0.05) between model control group and two treated groups at any time points. AST and BUN serum contents have no marked
difference (P > 0.05). ALT serum contents in S. miltiorrhiza-treated group (6 and 12 h) and scutellarin-treated group (12 h) are obviously less than those in model control group (P < 0.05). The serum contents of Cr and amylase in scutellarin-treated group (6 h) are obviously less than those in model control
group (P < 0.05). There is a different degree of relief on the pathologic changes of multiple organs in the two treated groups compared
with those in model control group, of which pancreas and liver’s pathologic severity scores in scutellarin-treated group (6
and 12 h) have reduced (P < 0.01) significantly compared with those in the model control group. However, there are no significant differences between
scutellarin-treated group and S. miltiorrhiza-treated group (P > 0.05). We think the scutellarin parenteral solution has a certain protective effect on SAP rats’ multiple organ injuries. 相似文献
8.
9.
10.
Ikechukwu R. Obidike Lawrence O. Aka Wilfred S. Ezema 《Comparative clinical pathology》2011,20(6):625-630
This study investigated the effects of caffeine extract from kola nut on body weight, hematological indices, serum enzyme
activities, and testicular morphology and function in male albino rats. Ten rats from a total of 58 rats used for this study
were used for the assessment of baseline body weight, hematological indices, serum enzyme, and sperm reserve values. Later,
three groups of 12 rats (groups A, B, and C) received 10, 20, and 30 mg/kg body weight dose levels of caffeine extract daily,
respectively, via the intraperitoneal route for 30 days. The group D rats (n = 12) served as the control and received no caffeine extract treatment. Administration of caffeine extract from kola nut
in rats led to significant (P < 0.05) reduction in mean body weight, packed cell volume, erythrocyte count, mean corpuscular hemoglobin concentration,
and hemoglobin concentration. Mean total leukocyte count did not differ significantly (P > 0.05) in the three treatment groups relative to the control. Increasing the dose levels of the caffeine extract (10, 20,
and 30 mg/kg body weight) significantly (P < 0.05) elevated the mean aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase values. Exposing
rats to graded dose levels of caffeine extract led to a significant (P < 0.05) reduction in both gonadal and extra-gonadal sperm reserves. Liver sections of rats that received 30 mg/kg body weight
of caffeine extract revealed enlarged portal ducts with cellular proliferation around the portal duct. The results of this
study have not only shown the adverse effects of caffeine extract from kola nut in rats but have also provided additional
knowledge and information to the existing pathophysiological implication of caffeine intake. 相似文献
11.
Few data are available on the kinetic of the airways’ inflammation induced by inhaled endotoxin in a given subject. The purpose
of this study was to evaluate in healthy subjects the time-related endotoxin-induced airways’ inflammation. The cells counts
from the induced-sputum were evaluated before, 6 and 24 h, and 7 days after an exposure to 20 mcg inhaled endotoxin, in eight
pre-selected volunteers. To avoid interference of the induced-sputum procedure on the response to endotoxin, each time-point
was evaluated in randomized order at 2-weeks interval after three separate inhalations of endotoxin. A significant rise of
the relative number of lymphocytes (p < 0.05) and polymorphonuclear neutrophils (PMN; p < 0.02) and of the absolute number of PMN (p < 0.05) occurring at 6 h, followed by an increase of the absolute number of the total viable cells (p < 0.01), macrophages (p < 0.001), neutrophils (p < 0.01), and lymphocytes (p < 0.05) at 24 h after endotoxin inhalation. The inflammatory response recovered totally after 7 days. In human beings, the
inhalation of endotoxin induced a transient airway inflammation after 6 h, peaked at 24 h and recovered after 7 days. When
repeated endotoxin inhalations are used as a model of inflammation, a wash-out period of at least 7 days should be applied
between each exposure in each subject. 相似文献
12.
Samantha W. M. Lun C. K. Wong Fanny W. S. Ko David S. C. Hui Christopher W. K. Lam 《Journal of clinical immunology》2009,29(3):330-342
Background We investigated the expression profile of toll-like receptors (TLRs) and TLR ligand-activated production profile of asthma-related
inflammatory cytokines in asthmatic patients. The expression of TLR1–8 on monocytes, CD4+ T helper lymphocytes, CD8+ T cytotoxic
lymphocytes, CD19+ B lymphocytes, and dendritic cells, and ex vivo production of cytokines from peripheral blood mononuclear
cells activated by TLR ligands were measured by flow cytometry.
Discussion Ex vivo productions of TNF-α, IL-10, and IL-1β by TLR4 and TLR5 ligand LPS and flagellin were significantly lower in asthmatic
patients (all P < 0.05). Expression of TLR4 and TLR5 was also found to be significantly lower in asthmatic patients when compared to that
of control subjects (all P < 0.05). Therefore, the decreased activation of TLR4 and TLR5 in asthmatic patients might contribute to the immunopathological
mechanisms of asthma by reducing the release of Th1 and anti-inflammatory cytokines.
Samantha W. M. Lun and C. K. Wong contributed equally to this study. 相似文献
13.
Behzad Mehdi Khabazian Abbass Ghanbari-Niaki Ali reza Safarzadeh-Golpordesari Mehdi Ebrahimi Fatemeh Rahbarizadeh Hossein Abednazari 《European journal of applied physiology》2009,107(3):351-358
The purpose of this study was to investigate liver and intestinal ABCA1 expression and plasma HDL-C level in response to treadmill-running
training in rats. Twenty adult Wistar male rats (17–18 weeks old, 300–322 g) were divided into control (n = 10) and Training (n = 10) groups. Training group trained at 25 m/min (0% grade) for 60 min/day, 5 days/week for 12 weeks. Rats were killed 48 h
after the last session of training. The intestinal and liver ABCA1 mRNA expression was found to be significantly higher in
trained compared to control group (P < 0.006 and P < 0.024, respectively). Intestine and liver ATP concentrations remained unchanged. Plasma HDL-C, HDL2-C, Apo A-1, pre-β HDL-C
concentration, LCAT activity, TC/HDL-C and LDL-C/HDL-C ratio significantly increased in trained group (P < 0.01, P < 0.006, P < 0.001, P < 0.001 P < 0.067, P < 0.02, and P < 0.03, respectively). However, other lipoprotein concentrations were unchanged. In conclusion, we found that endurance training
induced significant elevation in plasma HDL-C and HDL2-C concentrations, accompanied by higher plasma Apo A-1, pre-β HDL-C
concentrations, LCAT activity and ABCA1 mRNA expressions in rat intestine, and liver. 相似文献
14.
Catta-Preta M Mendonca LS Fraulob-Aquino J Aguila MB Mandarim-de-Lacerda CA 《Virchows Archiv : an international journal of pathology》2011,459(5):477-485
The issue of adequately quantitatively evaluating hepatic steatosis is still unresolved. Therefore, we compared three methods
of quantitative assessment. Two groups of mice (n = 10 each) were fed standard chow (10% fat, SC group) or a high-fat diet (60% fat, HF group) for 16 weeks, and hepatic triglyceride
(HT) and liver tissue were then studied. Paraplast-embedded tissues stained by hematoxylin and eosin (H-E) were compared to
frozen sections stained by Oil Red-O (ORO). In addition, the volume density of steatosis (Vv[steatosis, liver]) was measured
by point counting (P-C, sections H-E or ORO) or by image analysis (I-A, sections ORO). HT was significantly higher in the
HF group (104% greater, P = 0.0004) than in the SC group. With P-C and H-E, Vv[steatosis, liver] was 4.80 ± 0.90% in the SC group and 33.50 ± 3.17%
in the HF group (600% greater, P < 0.0001). With P-C and ORO, Vv[steatosis, liver] was 4.86 ± 0.89% in the SC group and 25.21 ± 1.27% in the HF group (420%
greater, P < 0.0001). With I-A and ORO, Vv[steatosis, liver] was 4.17 ± 0.85% in the SC group and 23.35 ± 1.58% in the HF group (460%
greater, P < 0.0001). Correlations between Vv[steatosis, liver] and HT were strong and significant in all methods. In conclusion, all
methods were appropriate and reproducible. In P-C and H-E, there is a slight overestimation of steatosis in the HF animals
in comparison to frozen sections and ORO; in frozen sections, differences between P-C and I-A are insignificant. 相似文献
15.
I. O. Igbokwe L. B. Buratai U. L. Ubah A. Aromnde N. A. Igbokwe 《Comparative clinical pathology》2009,18(2):191-195
Trypanosoma brucei, Federe strain, caused an acute infection in rats after intraperitoneal inoculation of 106 trypanosomes. Parasitemia occurred from day 3 post-infection (pi) with peak parasitemia from day 7 pi. Anemia was observed
between days 7 and 11 pi. The serum triglyceride concentration was comparable with the control value on day 7 pi, but increased
(P < 0.05) above the control value on day 11 pi. The serum total cholesterol, high density lipoprotein (HDL), and low density
lipoproteins (LDL) cholesterol concentrations decreased (P < 0.0.05) when compared with the control values on days 7 and 11 pi. The LDL cholesterol decreased more on day 11 than day 7
pi. The liver content of triglycerides and total cholesterol decreased (P < 0.05) during the infection from control values on days 7 and 11 pi. The decrease in hepatic triglyceride concentration
was more on day 11 than day 7 pi, while the hepatic total cholesterol content decreased to comparable extents on days 7 and
11 pi. Hepatic LDL cholesterol content was unaffected on day 7 pi, but decreased (P < 0.05) on day 11 pi. The content of HDL cholesterol in the liver did not vary (P > 0.05) significantly during the infection. It was concluded that the decreased hepatic contents of these lipids were consistent
with the serum lipid concentration, which did not seem to favor lipid uptake by hepatocytes. 相似文献
16.
Aloysius MM Zaitoun AM Beckingham IJ Neal KR Aithal GP Bessell EM Lobo DN 《Virchows Archiv : an international journal of pathology》2007,451(5):943-948
FOLFOX-4 (folinic acid/5-fluorouracil/oxaliplatin) chemotherapy is used to treat patients with colorectal liver metastases. We aimed to assess hepatic histopathological
responses to neoadjuvant FOLFOX-4 chemotherapy in patients with colorectal liver metastases. We selected all patients (n = 54) treated with FOLFOX-4 for colorectal liver metastases between June 2002 and June 2005. Only 25 underwent hepatectomy
and formed the study group. Histological responses were assessed in the study group and a matched control group (n = 25) that did not receive neoadjuvant chemotherapy. The median (IQR) body mass index in the study and control groups was
24 (22–26) and 24 (23–25) kg/m2, respectively, (P = NS). Complete histological resolution of tumour occurred in six (24%) patients in the study group. Median residual tumour
cellularity was less (35 vs 70%) and fibrosis greater (50 vs 5%) in patients in the study group when compared with controls
(P < 0.001). The liver surrounding the tumour was steatotic in 17 (68%) patients in the study group and five (20%) controls
(P = 0.001). Hepatic sinusoidal dilatation was more pronounced in patients in the study group than in controls (P < 0.001). The response to FOLFOX-4 was associated with tumour necrosis, fibrosis and inflammation. More than two thirds of
patients undergoing hepatectomy after FOLFOX-4 had steatosis despite being non-obese. 相似文献
17.
Bahcecioglu IH Koca SS Poyrazoglu OK Yalniz M Ozercan IH Ustundag B Sahin K Dagli AF Isik A 《Inflammation》2008,31(4):215-221
To assess the effect of infliximab, an anti-tumor necrosis factor (TNF)-α agent, on the carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Rats were randomized into three groups (n = 9). The control group received only intraperitoneal (i.p.) olive oil. Hepatic fibrosis was induced by repeated i.p. injections
of 1.5 ml/kg CCl4 (1:3 mixture with olive oil) for 5 weeks in the remaining two groups which were also injected subcutaneous saline or 2 mg/kg
infliximab. Infliximab reduced the levels of aspartate aminotransferase and alanine aminotransferase (p < 0.05 for both). The scores of hepatic necrosis, inflammation and fibrosis, and expression of α-smooth muscle actin were
lower in the infliximab-treated group than the CCI4-treated group (p < 0.01, p < 0.001, p < 0.01, p < 0.001, respectively). However, there was no significant difference in terms of liver tissue and plasma malondialdehyde,
and serum TNF-α levels, while infliximab relatively reduced the level of transforming growth factor-β1 (373.0 ± 153.1 vs. 280.8 ± 127.1 pg/ml). Treatment with infliximab attenuated the necro-inflammation and fibrogenesis in
the CCI4-induced hepatic fibrosis, and thus it might be effective as a therapeutic anti-fibrotic agent. 相似文献
18.
An experimental study was performed to determine the effect of gasoline inhalation on sex hormones and offspring sex ratio
in male rats. Twenty two Sprague–Dawley adult male rats were divided into two groups. In the first group (n = 11), animals were exposed to gasoline vapor for 6 h daily for 30 consecutive days. The second group served as control without
gasoline exposure. In the end of the study, sex hormones including total testosterone, luteinizing hormone and, follicle-stimulating
hormone were determined using radioimmunoassay methods. Then, animals in both groups were mated with healthy unexposed female
rats and sex of offspring was determined after birth. Results showed that the level of all sex hormones was significantly
decreased in test group compared with control ones (P < 0.02). Offspring sex ratio was 0.39 (38/96) in the test group, significantly lower than 0.54 (60/101) in the control group
(P = 0.03). The potential of manipulating offspring sex ratio through a simple, safe, and practical method would be an interesting
subject. The results of the present study could provide a baseline for future research on this subject. 相似文献
19.
In vivo evaluation of the improved MCMS-0102 pacemaker with a rapid pacing mode for induction of experimental heart failure in animals 总被引:2,自引:0,他引:2
Kuniyoshi Watanabe MD Hideo Kuroda MD PhD Eiichi Sato PhD Hideo Makino PhD 《Journal of artificial organs》2006,9(2):84-89
The MCMS-0102 cardiac pacemaker for rapid ventricular pacing to induce heart failure in animals has been improved in terms
of miniaturization and performance. To determine the performance of the new MCMS-0102, six devices were implanted in beagle
dogs, and two of these devices were reimplanted for continued pacing in a total of eight beagle dogs. The hearts were paced
at 260 beats per minute for 4 weeks (P group: n = 8). The hemodynamic status of the P group was examined and compared with nonpaced dogs (NP group: n = 8). The neurohumoral status of the P group was evaluated before and after rapid pacing. Stable operation of the six devices
during rapid pacing was confirmed using the telemetry system. Postmortem examinations revealed features similar to clinical
heart failure characterized by massive ascites, pleural effusion, cardiomegaly, and liver congestion in all the paced dogs.
Cardiac output was 1.1 ± 0.2 l/min in the NP group and 0.5 ± 0.1 l/min in the P group (P < 0.0001). The left atrial pressure and the central venous pressure of the P group and the NP group were 23 ± 6 versus 6
± 2 mmHg (P < 0.0001) and 10 ± 3 versus 4 ± 3 mmHg (P < 0.001), respectively. In the paced dogs, plasma renin activity increased from 0.5 ± 0.4 to 8.5 ± 7.4 ng/ml/h (P < 0.05) and atrial natriuretic peptide levels increased from 69 ± 41 to 229 ± 72 pg/ml (P < 0.001). The improved MCMS-0102 was successfully implanted in beagle dogs and it succeeded in inducing the congestive heart
failure model. 相似文献
20.
The aim of our study was to evaluate the pro- and anti-inflammatory cytokine response during acute pancreatitis and its predictive
value on severity of disease. A hospital-based prospective clinical study was conducted. Twenty patients with acute pancreatitis
were enrolled during a 12-month period. Plasma concentrations of TNF-α, IL-1β, IL-6, and IL-10 were determined at days 1,
2, 3, 6, and 9. The patient population was analyzed by type of acute pancreatitis. Severity was defined according to the Atlanta
criteria for assessing severity of acute pancreatitis. Clinical variables were recorded to patients classified in one of two
groups: severe acute pancreatitis (SAP group) and mild acute pancreatitis (MILD group). Patients with SAP had significantly
higher average levels of IL-6 compared to the MILD group patients (539.2 pg/L vs. 23.4 pg/L, p < 0.0001). Also, the values of IL-10 were significantly higher in patients with SAP (242.4 pg/L vs. 8.1 pg/L, p = 0.003). The values of TNF-α were not significantly different in both groups. The value of IL-6 and IL-10 showed a positive
correlation (r = 0.7964, p < 0.0001). Although a relatively small sample of patients was used, we can conclude that the determination of the value of
IL-6 and IL-10 can help in the clinical assessment of disease severity. 相似文献