IGSS和Dot—IGSS检测SLE病人血清中抗体的临床意义

目的 探讨免疫金银染色法和斑点免疫金银染色法检测系统性红斑狼疮病人的血清抗体临床意义。方法 用免疫金银染色法检测抗核抗体，用斑点免疫金银染色法检测抗双链ＤＮＡ抗体和抗肾小球基底膜抗体，检测４０例ＳＬＥ病人和正常人血清。结果 患者血清中抗核抗体、抗双链ＤＮＡ抗体和抗肾小球基底膜抗体阳性率分别为１００％、８７．５％和７２．５％，正常人无１例阳性。结论 ２种方法检测性红斑狼疮病人血清抗体有高度特异性和和     

副肿瘤性天疱疮患者的脱发机制

目的探讨副肿瘤性天疱疮(PNP)患者血清中抗桥粒芯蛋白(DSG)抗体与头皮脱发之间的关系.方法收集我科收治的2例PNP,2例大疱性类天疱疮患者和5例正常人血清,及2例正常人头皮组织.采用酶联免疫吸附(ELISA)方法检测PNP患者血清中DSG 1和3抗体水平,间接免疫荧光法检测PNP患者血清与正常人头皮毛囊结合情况,同时采用正常人血清和大疱性类天疱疮患者血清作对照.结果2例PNP患者中,有1例抗DSG 3抗体强阳性,抗DSG1抗体弱阳性;另1例患者两种抗体均为阴性.2例患者血清均可与正常人头皮毛囊结合,表皮细胞间和毛囊的外毛跟鞘均可见荧光信号.大疱性类天疱疮患者血清只能与表皮基底膜带结合,正常人血清在基底膜带、表皮细胞间和毛囊均无任何荧光信号.结论PNP患者出现脱发可能与患者血清中抗DSG3抗体水平有关.     

丙型肝炎患者肝特异性自身抗体的研究

目的:检测丙型肝炎患者血清肝特异性自身抗体并分析其临床意义.方法:以HEP-2细胞、猴肝、大鼠胃和大鼠肾组织为抗原,采用间接免疫荧光法对67例丙型肝炎患者血清、32例甲型肝炎、50例其它疾病和30例正常人血清作抗核抗体、抗平滑肌抗体、抗线粒体抗体和抗肝肾微粒体抗体检测.结果:丙型肝炎患者自身抗体总阳性率为25.4%,均高于其余3组对照组(P＜0.05).自身抗体以低滴度为主,主要为抗核抗体.自身抗体的阳性与患者的性别无关(P＞0.05),与年龄有关(P＜0.05).结论:HCV感染能诱导自身免疫反应使患者血清出现多种自身抗体,检测其自身抗体及其滴度对丙型肝炎患者的治疗有一定的参考价值.     

高血压病肾损害血清抗AT_1、α_1、β_1、M_2受体自身抗体临床研究

目的探讨血清AT1、α1、β1、M2-受体自身抗体与高血压病肾损害的关系及干预治疗效果。方法检测58例高血压病肾损害患者、60例高血压病无肾损害患者、50例正常人AT1、α1、β1、M2-受体自身抗体及早期肾功能损害指标,高血压患者均给予氯沙坦治疗。结果肾损害患者血清AT1、α1、β1、M2-受体自身抗体阳性率明显高于无肾损害患者,氯沙坦降压作用明显,能显著改善肾功能。结论血清抗AT1、α1、β1、M2-受体自身抗体可能与高血压病肾损害有关,氯沙坦能降低血压,可减轻和延缓高血压病引起的肾损害。     

血清抗果蝇唾腺染色体抗体测定及其临床意义

以果蝇唾腺染色体作为基质,用间接免疫荧光法检测血清抗果蝇唾腺染色体抗体(AchrA)。系统性红斑狼疮(SLE)患者44例,AchrA阳性者占16例(86.4％),而正常人及其它风湿性疾病(ORDs)患者共151例,均为阴性。提示AchrA对SLE有高度特异性及一定的敏感性。对比SLE患者的血清AchrA及抗核抗体(ANA).双阳性者占15例(84.1％)。提示与两种抗体起反应的核成分不尽相同。     

风湿性疾病的抗核抗体谱检测及意义

已知自身抗核抗体对不同风湿性疾病的诊断与鉴别诊断具有重要意义。本文采用免疫印迹技术（IBT），从分子水平对245例各种风湿性疾病和38份正常人血清进行抗可提取性核抗原（ENA）抗体检测，并同时测定抗双链DNA抗体，抗核抗体作对照。结果表示，抗Sm,抗核糖体，抗双链DNA抗体是系统红斑狼疮的具有高度特异性的自身抗体；而抗Scl-70,抗Jo-1,抗SSB抗体则分别是进行性系统性硬化症，多发性肌炎，皮肌炎，干燥综合征的标记性抗体。抗核抗体在抗Sm抗体阴性的红斑狼疮患者中，阳性率显著高于抗双链DNA抗体，所以对这类患者的诊断有所帮助。     

自身抗体谱在肺癌患者诊断中的临床意义

目的 了解肺癌患者体内自身抗体阳性率、细胞内定位及荧光模式特点,分析自身抗体谱与肺癌的相关性.方法 收集36例病理诊断为原发性肺癌患者的血清,其中16例尚未行手术及化疗药物治疗,20例已行手术切除且正处于化疗阶段;另外收集47例正常人血清用于对照.采用间接免疫荧光法检测抗核抗体,蛋白质印迹法检测抗ENA抗体谱.结果 原发性肺癌患者组血清自身抗体阳性检出率高于对照组(P＜0.05);术前肺癌患者和术后肺癌患者自身抗体阳性检出率无统计学差异(P＞0.05);肺癌患者和正常对照组在自身抗体荧光模式、抗ENA抗体谱上均存在差异.结论 肺癌患者血清中存在自身抗体,不论是手术切除前还是术后化疗阶段均可检出;其自身抗体谱与正常人生理性自身抗体谱有一定差异,进一步研究其靶抗原本质及生物学意义,有助于研究肺癌患者自身免疫现象,并可能用于肺癌的辅助诊断.     

抗ANA多肽抗体谱检测对系统性红斑狼疮的诊断意义

目的探讨抗可提取性核抗原(ANA)多肽抗体谱检测对系统性红斑狼疮的诊断价值。方法对50例SLE患者和20例正常人血清标本采用实免疫印迹法检测抗ANA抗体。结果50例SLE患者血清中存在的自身抗体主要有抗dsDNA、抗ulRNP、抗Sm、抗SSA、抗Ro-52、抗SSB等6种抗体,阳性率分别为70.0%、34.0%、46.0%、58.0%、52.0%和42.0%。结论抗ANA抗体阳性谱的分布有一定的倾向性,其抗体谱检测对提高系统性红斑狼疮的诊断水平有重要意义。而且具有操作简便快捷,重复性好等优点,适宜在各级医院推广应用。     

天然DNA和细胞因子对SLE患者淋巴细胞体外分泌抗双链DNA抗体的影响

我们检测了6例活动期、6例缓解期SLE患者和10例正常人血清和外周血单个核细胞(PBMC)自发及经天然DNA(nDNA)、rIL—2、rIL—6和rIFN—γ诱导后分泌IgG类抗双链DNA(dsDNA)特异性抗体水平,观察到活动期患者血清和自发分泌量显著高于缓解期,后者又显著高于正常人,且体外细胞分泌平与血清中的含量密切相关(r=0.9882)。nDNA能特异地激发SLE患者的FBMC分泌抗dsDNA抗体,但对正常人无作用。rIL—2和rIFN—γ皆能增强SLE患者PBMC产生抗dsDNA抗体,表明即使缓解期病人,血中仍有表达IL—2受体的激活的B细胞存在。nDNA和rIL—2联合应用时,对活动期患者PBMC分泌抗dsDNA抗体有协同作用。本文未见外源性rIL—6对抗dsDNA抗体分泌的影响。     

ENA抗核抗体谱的检测在临床中的应用

目的 :了解抗可提取性核抗原的检测在临床上的意义。方法 :本文采用免疫印迹技术 ,对 2 17例各种疾病和 4 0份正常人血清进行抗可提取性核抗原抗体检测。结果 :某些抗体对疾病有高度特异性。如抗 Sm抗体对系统性红斑狼疮高度特异 ;而抗 Scl- 70、抗 Jo- 1、抗 SSB抗体则分别是系统性硬化症、多发性肌炎和皮肌炎、干燥综合征的标记抗体。结论 :ENA抗核抗体谱的检测对不同风湿疾病的诊断和鉴别诊断具有重要意义 ,通过反复检测随访追踪有利于发现早期不典型病例     

A NEW METHOD FOR DETERMINATION OF ANTI-DOUBLE STRAND DNA ANTIBODIES TRYPANOSOMA EQUIPERDUM IMMUNOFLUORESCENT METHOD

A new method of determining anti-double strand DNA antibody by blood smears of mice infected with Trypanosoma equiperdum used as a substrate for immunofluorescent technic is described. Sera from 547 persons, including 62 patients with systemic lupus erythematosus (SLE), 68 with other connective tissue diseases (CTD), 288 with miscellaneous diseases, and 129 healthy medical workers and blood donors were determined. The results were all 20 patients with active SLE showed strongly positive reac- tions; only 4 0f 42 patients with inact.ive SLE showed weakly positive react.ions; all other CTD patients except l and all patient.s with miscel- laneous diseases as well as healthy controls showed negative reactions. This test demonstrates very good correla- tion in comparison with the Farr assay and has some advantages over it, such as simplicity of performance, reliability and constancy of results and SLE specificity. seems applicable to the Moreover, the method detection of antinuclear antibody (ANA) under certain conditions. It is of value in diagnosis and follow up of SLE.     

海南岛流行性出血热血清学调查

目的 :调查海南岛一般人群中流行性出血热 (EHF)的隐性感染率 ,以了解人群对 EHF的免疫力。方法 :应用 m IFA法分别对采自海南各地区一般人群血清 3 71份、不明热患者血清 3 5份进行流行性出血热 (EHF) Ig G抗体检测。结果 :一般人群血清中只有 1份阳性 (滴度 :1∶ 80 )。阳性率为0 .2 7% ,不明热患者血清中有 2份阳性 (滴度 :1∶ 2 0 )。阳性率为 5 .71 %。结论 :海南岛一般人群隐性感染率低 ,人群缺乏对 EHF的免疫力。海南岛是否存在 EHF的易感因素需要进一步研究。     

Clinical and laboratory characteristics of patients with speckled pattern antinuclear antibodies

Fifty patients whose sera contained a speckled antinuclear antibody (ANA) were interviewed and examined to determine if there was any relationship between their clinical manifestations and the presence of certain serological markers. The results suggest that speckled ANA is usually found in patients with definite connective tissue diseases, but a significant minority have incomplete or early stages of these diseases. Characterisation of the antibody to extractable nuclear antigen (ENA) and other serological markers does not normally assist in making a clinical diagnosis, but the detection of a speckled ANA should prompt further investigation and careful follow-up.     

Immunization status of thirty patients with sickle cell disease five years post hepatitis B vaccination. A comparison with normal controls

In order for hepatitis B immunization programmes to be cost effective and clinically beneficial, vaccinated persons should maintain an immunity threshold titre of antibodies to hepatitis B surface antigen greater than 10 IU/l. Those who fall below this level should be boosted in order to be covered against the risk for which the vaccine was administered. Persons with sickle cell disease are included in the group for whom hepatitis B immunization is routinely prescribed. Antibody to hepatitis B surface antigen was measured in paired sera of thirty patients with sickle cell disease compared with a control group of healthy medical staff, five years post vaccination. There was no significant difference between patients with sickle cell disease and normal controls in the levels of antibody maintained or numbers that required booster vaccination. Recommendations for the maintenance of protection via revaccination should be the same for persons with sickle cell disease as for healthy persons.     

SEROEPIDEMIOLOGIC STUDY OF EPIDEMIC HEMORRHAGIC FEVER WITH RENAL SYNDROME IN CHINA

Sera from 193 cases of epidemic hemorrhagic fever renal syndrome (EHFRS) and 173 normal persons and patients with other diseases in endemic and nonendemic areas were examined for immunofluorescent (IF) antibodies to Korean hemorrhagic fever (KHF) antigen by indirect immunofluorescent antibody (lFA) test. 76 0f 78 (97.4470) paired sera from EHFRS patients were KHF' antibody positive in l or both samples. Of 109 acute stage sera samples 81 (74.31To) were KHF IgM antibody positive whereas of 162 con- valescent sera samples 156 (96.2970) were KHF IgG antibody positive, indicating that the EHFRS agent is immunologically related, if not identical with KHF. The earliest date of IF IgM antibody ap- pearance was l day after onset of illness a.nd IF IgG antibodies could be found in the patient convalescent serum 16 years after the acute at- tack. There was no immunologic relationship between the EHFRS agent and Japanese B en- cephalitis (JBE) or influenza A3 viruses.     

Antineutrophil cytoplasmic antibody in patients with antinuclear antibody-positive chronic hepatitis C

Hepatitis C viral (HCV) infection has been shown to lead to autoimmune phenomena. Antineutrophil cytoplasmic antibodies (ANCA) have been known to be present in several autoimmune liver diseases. This study aimed to evaluate the prevalence of ANCA in sera of patients with antinuclear antibody (ANA)-positive chronic hepatitis C (CH-C) and to characterize ANCA antigens by Western blot compared with HCV-negative autoimmune hepatitis (AIH). METHODS: Ninety sera obtained from 20 patients with ANA-positive CH-C, 20 patients with ANA-negative CH-C, 20 patients with AIH, 6 patients with primary screlosing cholangitis (PSC) and 24 healthy controls were tested using an indirect immunofluorescence assay, cell ELISA and Western blotting to detect ANCA. RESULTS: In the indirect immunofluorescence assay, ANCA was found in 60% (12/20) of patients with ANA-positive CH-C, 100% (20/20) of patients with AIH and 33.3% (2/6) of patients with PSC, but in none of patients with ANA-negative CH-C (n = 20) or healthy controls (n = 24). The staining pattern observed in sera of these patients was a peculiar mixture of both cytoplasmic and perinuclear patterns. The mean ANCA titer by cell ELISA was significantly (p < 0.01) higher in patients with AIH compared with ANA-positive or ANA-negative CH-C patients or healthy controls. However, no correlation was found between ANCA titers and ANA titers, serum alanine amino transferase (ALT) or IgG levels in either ANA-positive CH-C or AIH. Western blots of ANCA-positive sera from ANA-positive CH-C patients revealed two bands corresponding to molecular weights of 72 and 46 kDa and there was no differencies in ANCA antigens compared with that from AIH patients. CONCLUSIONS: We found an increased incidence of ANCA in ANA-positive CH-C patients. ANCA in ANA-positive CH-C patients is thought to be one of autoimmune phenomena lead by HCV infection because no difference was observed in the epitope of ANCA antigens between patients with CH-C and AIH.     

牛丝虫成虫抗原间接荧光抗体试验诊断丝虫病的实验观察

本文进一步用牛丝虫成虫抗原IFAT检测马来丝虫微丝蚴血症者69例,晚期病人42例,其IFAT阳性率分别为88.1％和85.7％。223例非丝虫病流行区健康者假阳性率是3.8％,对肠蠕虫感染者,过敏性哮喘,荨麻疹,过敏性疾病伴肠蠕虫感染者及急性肝炎者有一定的交叉反应。牛丝虫成虫抗原IFAT的敏感性高于寄生虫学检查,可作丝虫病的辅助诊断。     

血清唾液酸含量对恶性肿瘤诊断价值的探讨

本文通过对９７例恶性肿瘤患者及５０例正常人的血清唾液酸含量进行测定分析，血清唾液酸含量在恶性肿瘤患者有明显增高，手术后明显降低。提示血清唾液酸是恶性肿瘤的一种非特异性标志物，它可以用于癌症的普查、诊断、疗效的动态观察，是判断恶性肿瘤预后的辅助指示。     

间接免疫金银染色检测血清抗核抗体

应用间接免疫金银染色（ⅡGSS）检测了系统性红斑狼疮等自身免疫性疾病病人及正常人血清中的抗核抗体（ANA），并与间接荧光抗体试验（IFAT）作了比较，在149份血清中ⅡGSS检出45份阳性，IFAT只检出29份阳性。IFAT有32份为可疑，ⅡGSS只有2份为可疑，且ANA滴度以ⅡGSS为高，核型显示以ⅡGSS更清楚，结果表明，ⅡGSS检测血清ANA特异性高，较IFAT更敏感、可靠、并能反映ANA组分。     

Antinuclear antibodies and HLA class II alleles in Jamaican patients with systemic lupus erythematosus

OBJECTIVE: The relationship between human leukocyte antigens class II (HLA) and antinuclear antibodies was investigated in Jamaican patients with Systemic Lupus Erythematosus (SLE). METHODS: Samples of blood of 82 patients with SLE and 75 healthy controls were tested for antinuclear antibodies using the fluorescent antinuclear antibody (FANA) test, counterimmunoelectrophoresis (CIEP) and the Crithidia luciliae immunofluorescence test (CL-IFT). A DNA-based HLA typing method was used to determine the frequencies of alleles of HLA-DRB1, DRB3, DRB4 and DRB5 in patients and healthy controls. RESULTS: The FANA test was positive in all of the sera from patients with SLE. Anti-dsDNA antibodies were present in 49% (40/82), anti-Sm/RNP 44% (36/82) and anti-Ro/La 43% (35/82) of the sera from SLE patients. The frequency of HLA-DR4 was significantly lower in SLE patients than in healthy controls (2/82, 2% vs 15/75, 20%; RR = 0.12; p = 0.0004; CP = 0.005) but no other HLA-DRB1 SLE associations were found. A positive HLA-DR3 anti-Ro/La antibody association was found in the patients with SLE (9/21, 43% vs 5/55, 9%; odds ratio (OR) = 7.5; CP = 0.01). In contrast, possession of HLA-DR6 was negatively associated with the absence of anti-dsDNA antibodies (9/32, 28% vs 27/44, 61%; OR = 0.2; CP = 0.05). CONCLUSION: The HLA-DR6 allele is associated with the absence of antinuclear antibodies and HLA-DR3 with the presence of anti-Ro/La antibodies in Jamaican patients with SLE. However, these results and those of previous studies of Jamaican patients suggest that the HLA-DR3 association with the development of SLE reported in other populations might in fact reflect the association of HLA-DR3 with anti-Ro/La antibodies. Further investigations are needed to determine whether HLA-DRB antinuclear antibody associations define clinical subsets of SLE in Jamaican patients.