白藜芦醇下调NF-κB介导的MMP-9表达抑制人NCI-H446细胞的侵袭迁移

目的 观察白藜芦醇对肺癌细胞基质金属蛋白酶9(MMP-9)表达的影响,并探讨其可能的分子机制.方法 体外培养人小细胞肺癌NCI-H446细胞,用不同浓度的白藜芦醇刺激.小室侵袭实验检测NCI-H446细胞的迁移与侵袭;Western blot检测MMP-9表达以及NF-κB p65亚基核转位;荧光素酶报告基因分析MMP-9和NF-κB活性. 结果 50 nM白藜芦醇孵育24 h后,其迁移与侵袭率分别降低了37％和33％;Western blot显示,白藜芦醇能抑制p65核转位;此外,白藜芦醇能以剂量依赖性方式抑制MMP-9的转录活性,NF-κB突变后报告基因活性显著降低.结论 白藜芦醇可通过抑制NF-κB介导的MMP表达从而抑制NCI-H446细胞侵袭迁移.     

不同粒径汽车尾气颗粒物对A549细胞TNF-α等炎性因子及NF-κB的影响

目的 探讨并比较不同粒径汽车尾气颗粒物对人肺癌上皮细胞A549肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、核转录因子-κB(NF-κB)的mRNA及NF-κB蛋白表达的影响.方法 以不同粒径(0.18～3.2 μm)汽车尾气颗粒物混悬液(1 00 μg/ml)染毒A549细胞,48 h后RT-PCR法检测细胞TNF-α 、IL-6及NF-κB(P65)基因表达水平,Western Blot法检测胞浆及胞核内NF-κB(P65)蛋白表达水平.结果 与对照组相比,各染毒组细胞的TNF-α、IL-6及NF-κB(P65) mRNA表达水平均上升(P＜0.05),由高到低依次为0.56～、0.32～、0.18～、1.0～和1.8～3.2 μm组,两两之间(除0.18～和0.32～μ m组之间、1.0～和1.8～3.2 μm组之间)差异均有统计学意义(P＜0.05);各组TNF-α、IL-6 mRNA水平与NF-κB(P65) mRNA水平显著相关(r=0.944,0.890,P＜0.05).与对照组相比,除1.8～3.2 μm组无统计学意义外,其余各组胞浆NF-κB (P65)蛋白水平均降低(P＜0.05),由高到低依次为1.0～、0.18～、0.32～、0.56～μm组;而胞核NF-κB( P65)蛋白水平均升高(P＜0.05),由低到高依次为1.0～、0.18～、0.32～、0.56～μm组.结论 不同粒径汽车尾气颗粒物作用于A549细胞后均可使TNF-α、IL-6、NF-κB mRNA表达升高、NF-κB活化,活化的NF-κB由细胞质进入细胞核发挥其调控炎症反应的作用.各染毒组中以0.56～μm组炎性损伤效应最强,粒径是影响颗粒物毒性作用大小的重要因素之一.     

二氧化硅致人单核细胞THP-1核因子-κB活化定位改变的研究

目的 探讨矽肺发病过程中SiO2诱导对人单核细胞株THP-1核因子-κB(NF-κB)活化的影响。方法 采用间接免疫荧光细胞化学法结合激光共聚焦显微镜(LSCM，Zeiss 510)检测THP-1细胞NF-κB p65亚基(NF-κB／p65)定位；Western-blot法测定THP-1细胞核蛋白中NF-κB／p65水平。结果 免疫荧光分析显示，异硫氰酸荧光素(FTTC)标记的NF-κB／p65在胞浆区为绿色荧光，而进入了丙亚基碘(PI)红色荧光标记的胞核区时则红绿两色叠加成黄色荧光。正常细胞NF-κB／p65主要分布于胞浆区，核区很少，而100μg/mlSiO2刺激30min后NF-κB／p65荧光标记集聚于核区，胞浆区少见。Westem-blot结果显示，对照组(0μg/mlSiO2)THP-1细胞核蛋白中有低水平NF-κB／p65表达,100μg/ml和200μg/ml SiO2作用15min和30min，THP-1细胞核蛋白中NF-κB／p65表达增加，并随着剂量的增加和时间的延长，NF-κB／p65水平相对增加。NF-κB／p65激活剂脂多糖(LPS)处理的THP-1细胞NF-κB／p65定位情况和核蛋白水平与上述结果相似。结论 SiO2刺激可引起THP-1单核细胞NF-κB核转位活化。     

硒对甲醛致A549细胞激活蛋白-1和核转录因子-κB表达的影响

为探讨硒对甲醛致A549细胞激活蛋白-1(AP-1)和核转录因子-κB(NF-κB)表达的影响,以A549细胞株为实验对象,设对照组、0.1 mmol/L甲醛组、1.25 mg/L硒+0.1 mmol/L甲醛组、2.5 mg/L硒+0.1 mmol/L甲醛组、5.0 mg/L硒+0.1 mmol/L甲醛组、10.0 mg/L硒+0.1 mmol/L甲醛组分别进行染毒,采用蛋白免疫印记法检测核蛋白中AP-1、NF-κB的表达。结果显示,对照组A549细胞生长活跃,1.25、2.5、5.0、10.0 mg/L硒+0.1 mmol/L甲醛组染毒12、24、48 h后细胞生长受到抑制,且抑制率随硒浓度和染毒时间的增加而升高;0.1 mmol/L甲醛可使A549细胞核蛋白中AP-1和NF-κB的表达增加,而2.5、5.0、10.0 mg/L硒+0.1 mmol/L甲醛组可抑制AP-1和NF-κB的过度表达,与单纯甲醛染毒组比较,差异有统计学意义(P0.05)。提示硒在一定浓度下能够拮抗甲醛致A549细胞AP-1和NF-κB的表达。     

还原型谷胱甘肽对60Co γ射线激活核转录因子-кB影响研究

目的 探讨还原型谷胱甘肽(GSH)对<'60>Co γ射线引起的人肺腺癌A549细胞中p65蛋白表达情况的影响.方法 按处理方法不同将人肺腺癌A549细胞分为假照组、单纯照射组、GSH组、GSH+照射组.免疫印迹法检测各组细胞质和细胞核中p65蛋白的表达情况;免疫荧光法检测各组细胞中p65在细胞质和细胞核中的定位变化.结果 <'60>Co γ射线照射后,p65蛋白在细胞核中的表达量随着吸收剂量的增加逐渐增多;GSH+照射组与单纯照射组相比,细胞核中p65的表达明显减少;但p65在细胞总蛋白中的表达没有明显变化.免疫荧光法检测到单纯照射组与假照组比较,细胞核内p65的表达量增多;GSH+照射组细胞核内p65的表达量相对单纯照射组明显减少.结论 γ射线照射后人肺腺癌A549细胞核内p65的表达量增多,但GSH可以减少p65在细胞核内的表达,抑制p65的入核.     

泰斯巴汀对肺炎链球菌诱导的肺泡上皮细胞A549损伤的保护作用及机制研究

目的探讨泰斯巴汀对肺炎链球菌(SP)诱导的肺泡上皮细胞A549损伤的影响和其作用机制。方法将肺泡上皮细胞A549分为3组,依次为Control组、SP组和SP+泰斯巴汀组。MTT法检测细胞活力,流式细胞术检测细胞凋亡情况,Western blot检测凋亡相关蛋白(Bax和Bcl2)、炎性因子(IL-6和IL-10)以及NF-κB信号通路相关蛋白(NF-κB p65、NF-κB p-p65和TNF-α)的表达水平。结果 SP感染可抑制A549细胞存活,泰斯巴汀可减轻SP对A549细胞存活的抑制作用,并呈一定的浓度依赖性。SP组A549细胞凋亡率、Bax、IL-6、NF-κB p65、NF-κB p-p65和TNF-α的表达水平显著高于Control组,而Bcl2和IL-10的表达水平显著低于Control组。SP+泰斯巴汀组A549细胞凋亡率、Bax、IL-6、NF-κB p65、NF-κB p-p65和TNF-α的表达水平显著低于SP组,而Bcl2和IL-10的表达水平显著高于SP组,差异均有统计学意义(P0.05)。结论泰斯巴汀通过抑制NF-κB信号通路,能够抑制肺炎链球菌诱导的肺泡上皮细胞凋亡和促炎因子表达,对肺炎链球菌诱导的肺泡上皮细胞损伤具有保护作用。     

还原型谷胱甘肽对~(60)Coγ射线激活核转录因子-κB影响研究

目的探讨还原型谷胱甘肽(GSH)对60Coγ射线引起的人肺腺癌A549细胞中p65蛋白表达情况的影响。方法按处理方法不同将人肺腺癌A549细胞分为假照组、单纯照射组、GSH组、GSH+照射组。免疫印迹法检测各组细胞质和细胞核中p65蛋白的表达情况;免疫荧光法检测各组细胞中p65在细胞质和细胞核中的定位变化。结果 60Coγ射线照射后,p65蛋白在细胞核中的表达量随着吸收剂量的增加逐渐增多;GSH+照射组与单纯照射组相比,细胞核中p65的表达明显减少;但p65在细胞总蛋白中的表达没有明显变化。免疫荧光法检测到单纯照射组与假照组比较,细胞核内p65的表达量增多;GSH+照射组细胞核内p65的表达量相对单纯照射组明显减少。结论γ射线照射后人肺腺癌A549细胞核内p65的表达量增多,但GSH可以减少p65在细胞核内的表达,抑制p65的入核。     

核内巨噬细胞集落刺激因子对宫颈癌细胞增殖和侵袭能力的影响

目的研究细胞核内巨噬细胞集落刺激因子(M-CSF)对宫颈癌细胞增殖能力和侵袭能力的影响及其可能机制。方法以构建的核内稳定表达M-CSF的HeLa细胞系为模型, 溴脱氧尿苷(BrdU)标记的脱氧核糖核酸(DNA)复制分析法检测细胞增殖能力, Transwell小室检测细胞侵袭能力;蛋白免疫印迹(Western blot)检测细胞核蛋白核内转录因子κB p65(NF-κB p65)和细胞总蛋白基质金属蛋白酶2(MMP-2)的表达情况。结果核内稳定表达M-CSF的HeLa细胞的增殖能力和侵袭能力均明显增强(P<0.05, P<0.01), 且NF-κB p65、MMP-2表达增多(均P<0.05)。结论核内M-CSF可促进宫颈癌细胞的增殖和侵袭能力, 其机制与上调NF-κB p65和MMP-2表达有一定的关系。     

Trends in meningococcal disease in Italy in 1988

Meningococcal disease in Italy decreased 15% in 1988 from the previous year (290 vs. 342 cases). The decline was particularly evident in military cases (1.7/100,000 in 1988 vs. 5/100,000 in 1987) reflecting the full coverage of bivalent serogroup (A + C) meningococcal polysaccaride vaccine in army recruits, achieved since January 1988. The highest proportion of cases was seen in people older than 25 years of age (25%). Serogroup C constituted 60% of the isolates, while 19% belonged to serogroup B. The proportion of strains resistant to sulphonamides was 45%, while 15% were resistant to Minocycline and none to Rifampin. Out of the five military cases, only one (due to serogroup C) was attributable to the vaccine failure. A single coprimary case, but no secondary cases occurred among civilians. These findings are consistent with the trends reported in Italy in the previous years.     

Benzene in blood and phenol in urine in monitoring benzene exposure in industry

Determinations of benzene concentration in blood and of phenol in urine were made by head-space gas chromatography techniques on samples taken near the end of the work day from two groups of workers potentially exposed to low levels of benzene in the work-place atmosphere. Preliminary results suggest that benzene in blood is more reliable than phenol tests for assessing both exposure and uptake of benzene. Normal values of phenol in urine (10 mg/liter or less) were found in nearly all those cases in which benzene was detected in the blood.     

2015年郑州市饮用水放射性水平状况调查

目的了解郑州市不同地区、不同季节、不同水源类型的放射性水平。方法分别于丰水期和枯水期采集郑州市区黄河水源水、井水源水、丹江口水源水、出厂水和末梢水各一份;以县为单位,每单位采集出厂水、末梢水、水库水、河水、井水各1份,按《生活饮用水生活标准检验方法》(GB/T 5750.13-2006)检测饮用水中总α和总β放射性水平,依据《生活饮用水卫生标准》(GB 5749-2006)进行判定。结果丰水期、枯水期水体总α、总β放射性水平均低于国家标准限值。结论郑州市不同水源类型均处于正常的天然放射性本底水平。     

Occupational stress in nurses in psychiatric institutions in Taiwan

Nurses are known to be exposed to occupational stress. However, occupational stress is not well documented for nurses in psychiatric institutions in Taiwan. A cross-sectional study was conducted to explore the work-related stress and risk factors of nurses in psychiatric institutions in Taiwan. A structured questionnaire was distributed to nurses at five state-owned psychiatric hospitals in Taiwan in 2001. Demographic information, working environment, and personal health status were inquired. Occupational stress was assessed based on the Chinese version of Job Content Questionnaire (JCQ). General health status and mental health were evaluated by the International Quality of Life Assessment Short Form-36 (IQOLA SF-36). A total of 573 questionnaires were disseminated to nurses and 518 (90.4%) were satisfactorily completed by nurses, including 408 female full-time nurses who had been in their current work for more than 6 months. In the past one month, 17.2% of nurses reported being under significant stress often or always. Assault episodes were reported by 45.1% of nurses in the past 6 months. Among the nurses, 16.9%, 25.2%, 50.0%, and 7.8% belong to the "High strain", "Low strain", "Active", and "Passive" groups, respectively. Perceived occupational stress was associated with young age, widowed/divorced/separated marital status, high psychological demand, low workplace support, and threat of assault at work. Lower general health score was associated with low job control, high psychological demand, and perceived occupational stress. A lower mental health score was associated with low job control, high psychological demand, low workplace support, and perceived occupational stress. We concluded that nurses in psychiatric institutions are under significant stress related to work factors.     

Changes in nursing administration in supporting transplantation in Brazil

This historical and bibliographic study aimed to understand how Nursing was organized to support care in transplantation. The HISA, LILACS, BDENF, PERIENF and DEDALUS databases were consulted, and thirteen references were found, ten of which were scientific articles, two were master's dissertations and one was a doctoral thesis. The span of time chosen for study ranges from the date of the first kidney transplant in Brazil (1965), to the date of publication of the last scientific article found in the databases mentioned above (2003). After reading these articles, the ones that were similar in topic were grouped together, thus creating the thematic axis for the presentation of the results. The results showed that the Nursing profession has played an important and active role in transplants ever since the first procedure in 1965.     

2019年中国肿瘤流行病学研究进展

恶性肿瘤已成为中国居民的主要死因之一。近些年来,中国政府积极推进肿瘤预防和控制领域的研究工作,取得了很大的进步。本文简要汇总中国在肿瘤流行病学领域的研究进展,具体包括2019年的肿瘤负担、癌症危险因素及其干预、筛查和早期发现、癌症防治专项行动(2019-2022年)方案等方面,以期为我国肿瘤防治工作的有效开展提供技术支撑和理论依据。     

2012年绵阳市蔬菜中农药残留状况

目的 了解绵阳市2012年市售蔬菜中农药残留情况,为食用蔬菜监管提供依据.方法 全部样品均按照GB/T 5009-2003的方法进行农药的残留检测.依据GB 2763-2005《食品中农药最大残留限量》进行判定.结果 2012年共检测蔬菜165份,农药检出率和超标率分别为72.12％和23.64％.豆类、根茎类、叶菜类、瓜果菜类和食用菌类蔬菜检测农药残留超标率分别为8.00％、23.53％、36.00％、5.26％和27.03％,差异有统计学意义(x2=11.41,P＜0.01).不同种类农药超标率差异有统计学意义(x2 =62.42,P＜0.01).夏季和冬季蔬菜农药超标率差异无统计学意义(x2=3.44,P＞0.05).结论 绵阳市叶菜类蔬菜中农药残留情况较为严重,应采取有效措施,加强蔬菜的监督管理,从源头禁止高毒农药的使用,加大蔬菜种植、销售环节的监测,确保市民食用蔬菜的安全.