海马三肾丸补肾壮阳作用实验研究

目的:观察海马三肾丸的补肾壮阳作用,为临床用药提供科学依据.方法:通过对去势雄性大鼠各附性器官重量、雄性大鼠交配能力、阳虚症小鼠抗寒抗疲劳影响的实验研究,观察药物的补肾壮阳作用.结果:药效学实验结果表明海马三肾丸能明显增加阳虚症雄性小鼠耐寒、抗疲劳能力,对去势雄性大鼠各附性器官有明显增重作用,对正常雄性大鼠扑提、射精潜伏期有明显缩短作用.结论:海马三肾丸具有补肾壮阳作用,为临床用药提供科学依据.     

八仙丸胶囊的制备及其药效学实验

目的:考察八仙丸胶囊的壮阳作用.方法:采用果蝇及大、小白鼠对八仙丸胶囊壮阳作用进行药效学研究.结果:八仙丸胶囊能提高果蝇交配率,明显缩短去势大鼠电刺激诱发阴茎勃起的潜伏期,能增强耐力和耐缺氧能力.结论:八仙丸具有提高机体免疫力和补肾壮阳的作用.     

回春至宝口服液的药理作用研究

回春至宝口服液具有温肾壮阳起萎、补益气血、抗衰老功效。药理研究表明．本品能显提高小白鼠抗疲劳、耐缺氧的能力，明显增加“阳虚”小白鼠的肾上腺、精囊腺重量及升高血清睾酮的含量．急性毒性试验表明．相当于临床剂量300倍时．对小白鼠未发现任何毒性反应。     

国产鸸鹋蛋壳粉影响雄性小鼠性功能的初步观察

目的观察鸸鹋蛋壳粉对小鼠性功能的影响,对国产鸸鹋蛋壳的药理作用进行初步的实验研究。方法给成年雄性小鼠连续服药21d后采用小鼠交配试验测其交配的潜伏期及交配次数。给幼龄雄性小鼠连续服药30 d后检测其附性器官的脏器指数。以重复悬吊应激复制小鼠性行为障碍模型,观察雄性小鼠的交配能力。结果鸸鹋蛋壳粉能增加成年雄性小鼠的交配次数,促进未成年雄性小鼠生殖器官的生长发育,对重复悬吊应激引起的雄性小鼠性功能障碍具有一定的促进功能恢复的作用。结论国产鸸鹋蛋壳粉具有一定的增强小鼠性功能,提高小鼠性器官生长发育的作用。     

国产鸸鹋蛋壳粉影响雄性小鼠性功能的初步观察

目的观察鸸鹋蛋壳粉对小鼠性功能的影响,对国产鸸鹋蛋壳的药理作用进行初步的实验研究。方法给成年雄性小鼠连续服药21d后采用小鼠交配试验测其交配的潜伏期及交配次数。给幼龄雄性小鼠连续服药30 d后检测其附性器官的脏器指数。以重复悬吊应激复制小鼠性行为障碍模型,观察雄性小鼠的交配能力。结果鸸鹋蛋壳粉能增加成年雄性小鼠的交配次数,促进未成年雄性小鼠生殖器官的生长发育,对重复悬吊应激引起的雄性小鼠性功能障碍具有一定的促进功能恢复的作用。结论国产鸸鹋蛋壳粉具有一定的增强小鼠性功能,提高小鼠性器官生长发育的作用。     

三子强肾混悬颗粒补肾壮阳及抗疲劳作用的研究

目的：考察三子强肾混悬颗粒的补肾壮阳及抗疲劳作用,为其临床用药提供实验依据。方法：通过采用大鼠交配模型和对雄性去势大鼠模型的试验,观察其对大鼠交配能力和时去势大鼠的附性器官、性激素水平的影响。采用氢化可的松致小鼠贤阳虚模型观察其抗疲劳作用。结果：三子强肾混悬颗粒组与模型对照组比较,可显著缩短去势大鼠阴茎勃起潜伏期（P〈0.01）和显著增加大鼠血清睾酮水平（P〈0.01）,可显著增加精囊腺-前列腺,提肛肌质量（P〈0.01）,三子强肾混悬颗粒高剂量组可显著提高正常雄性大鼠的交配能力（P〈0.01）。中、高剂量组均有显著增加肾阳虚小鼠体重及自主活动能力和游泳时间的作用（P〈0.01）。结论：三子强肾混悬颗粒具有较好的补肾壮阳作用及抗疲劳作用。     

海洛因对小白鼠生育的影响

目的:研究海洛因依赖小白鼠的生育情况。方法:先腹腔注射海洛因,6天后腹腔注射盐酸纳洛酮进行催促实验,海洛因依赖模型建立后,分成二组:一组继续注射海洛因,一组停止注射海洛因,在d14,将已对海洛因产生依赖的♀♂小白鼠各10只,作为海洛因依赖组;已脱毒的♀♂小白鼠各10只,作为海洛因戒断组;已对海洛因产生依赖的♀小白鼠10只与NS组的♂小白鼠10只,作为海洛因♀依赖组;已对海洛因产生依赖的♂小白鼠10只与NS组的♀小白鼠10只,作为海洛因♂依赖组;剩余的NS对照组♀♂小白鼠各10只,作为NS对照组。各组♀♂分别配对,观察不同组别小白鼠生育情况。结果:海洛因依赖组、海洛因戒断组、海洛因♂依赖组、海洛因♀依赖组与NS对照组比较,有极显著差异(P<0.001),海洛因依赖组与海洛因戒断组比较有显著性差异(P<0.05),海洛因♂依赖组与海洛因♀依赖组比较,无显著性差异(P>0.05)。结论:海洛因对♀♂小白鼠的生育能力均有明显影响。     

二十八味补肾胶囊补肾壮阳作用研究

目的观察二十八味补肾胶囊补肾壮阳作用。方法通过雌鼠阴栓观察小鼠交配射精能力;采用去势制作大鼠肾虚模型,观察二十八味补肾胶囊的补肾壮阳作用。结果二十八味补肾胶囊能提高正常雄性小鼠的交配射精能力。对去势大鼠能缩短阴茎勃起潜伏期,增加包皮腺重量,对精囊腺和前列腺、提肛肌重量影响无显著性差异。结论二十八味补肾胶囊有一定的补肾壮阳作用。     

壮腰健肾液壮阳作用的实验研究

目的观察壮腰健肾液对大、小鼠的壮阳作用及其与剂量的关系,证实壮腰健肾液有壮阳作用,为临床应用提供实验依据。方法通过对去势小鼠性器官的影响、对大鼠性器官的影响;对棉油性致大鼠精虫数低下时精虫含量的影响以及对大鼠丘脑-腺垂体-肾上腺皮质轴的影响等实验,观察壮腰健肾液的壮阳作用。结果壮腰健肾液能显著增加去势小鼠性器官的重量和大鼠性器官的重量;显著提高棉油致大鼠精虫数低下时精虫的数量。结论壮腰健肾液有较强的壮阳作用。     

盐酸阿扑吗啡对大鼠性功能的影响

目的　探讨盐酸阿扑吗啡对大鼠性功能的影响。方法　将♂大鼠随机分组 ,分别注射不同剂量的盐酸阿扑吗啡(4 32、2 1 6、1 0 8mg·kg- 1 ) ,于d 1和d 8将♂大鼠单独投入观察笼中与♀大鼠进行交配实验 ,观察♂鼠的扑捉、射精潜伏期及 2 0min内扑捉♀鼠次数及射精次数 ,并在给药后d4和d 8取静脉血用放免法测定血中黄体生成素 (LH)、垂体催乳素 (PRL)、睾酮 (T)、及雌二醇 (E2 )。结果　盐酸阿扑吗啡 (4 32mg·kg- 1 和 2 1 6mg·kg- 1 )d 1、d 8♂鼠交配能力明显增强 ,合笼后扑捉雌鼠潜伏期及射精潜伏期明显缩短 ,2 0min内完成的扑捉及射精次数增加 ,对血中LH、PRL、T、E2值没有明显影响。结论　盐酸阿扑吗啡能明显增强♂大鼠的性交功能     

不同牛奶消费量对雄性ICR小鼠生殖系统的影响

目的研究不同牛奶消费量对雄性ICR小鼠生殖器官的影响。方法取ICR小鼠(21d)96只,雌雄各半。随机将实验动物分为4组:5ml牛奶组,10ml牛奶组,15ml牛奶组,对照组。小鼠在给予牛奶12周后进行组内雌雄1∶1同笼配对繁殖,同笼6d后处死亲代雄鼠,待子代小鼠断奶后处死亲代雌鼠。喂饲期观察ICR小鼠一般情况、体重、饲料摄入量、交配率、怀孕率,喂饲结束后处死小鼠,观察以下指标:精囊腺、附睾、睾丸的湿重与组织学观察,精子计数,放射免疫法检测血清中雌二醇、雌三醇、睾酮浓度。结果雄性ICR小鼠生殖器官未发现明显的增重或减重,生育能力及病理学观察也未见异常。结论牛奶对雄性ICR小鼠生殖系统无明显影响。     

干姜软膏的制备

目的：研究干姜软膏的制备。方法用水蒸气蒸馏法提取干姜的挥发油与樟脑,通过乳化方法制成O／W的软膏。结果该软膏细腻,稳定性好,易于涂布。结论该软膏制备工艺可行,性质稳定,具有一定的经济效益。     

Effects of cannabinoids given orally and reduced appetite on the male rat reproductive system

Chronic oral treatment of young adult male Fischer rats with delta 9-tetrahydrocannabinol (THC), 1, 5 and 25 mg/kg/day, or crude marihuana extract (CME), 3, 15 and 75 mg/kg/day, suppresses growth of accessory sex organs and body weight gain in a dose-related manner. Animals pair fed with the THC (25 mg/kg) group gained slightly more in body weight than the THC group, but their relative accessory sex organ weights were intermediate between THC and ad libitum-fed control group weights. These latter differences may be due to altered serum androgen levels since these levels 2-6 h after last treatment were 0.15, 0.77 and 3.33 ng/ml for THC, pair-fed and ad libitum-fed groups, respectively. 24 h after the last treatment all groups were within normal levels. Thus, chronic cannabinoid treatment suppresses accessory sex organ weights and serum androgen levels greater than the suppression caused by reduced food intake alone.     

TOXICITY EVALUATION OF PETROLEUM BLENDING STREAMS: REPRODUCTIVE AND DEVELOPMENTAL EFFECTS OF LIGHT CATALYTIC REFORMED NAPHTHA DISTILLATE IN RATS

A distillate of light catalytic reformed naphtha (CAS number 64741-63-5, LCRN-D) administered by inhalation was tested for reproductive and developmental toxicity in Sprague-Dawley rats, following a modified OECD Guideline 421, Reproductive/Developmental Toxicity Screening protocol. LCRN-D was administered as a vapor, 6 h/d, 7 d/ wk at target concentrations of 0, 750, 2500 or 7500 ppm to female rats for approximately 6 wk from 2 wk prior to mating, during mating through gestational d 19, and to males beginning 2 wk prior to mating for approximately 7 consecutive weeks. Dams and litters were sacrificed on postnatal d 4 and males were sacrificed within the week after the last litter was necropsied. Parental systemic effects observed at the 7500 ppm exposure level included slightly lower body weights for males throughout the study. Increased kidney to body weight and increased liver to body weight ratio in male rats exposed to 7500 ppm LCRN-D may be related to slightly lower final mean body weights. Body and organ weight data for female rats in all exposure groups were comparable to controls. No test-material-related microscopic changes were observed in the reproductive organs or nasal turbinate tissue of either sex. Reproductive performance was unaffected by exposure to LCRN-D. The mating and fertility indices were 100% in all groups. There were no significant exposure-related differences in implantation sites or live pups per litter, and no gross abnormalities were observed in pups from treated dams. Pups born from LCRN-D-exposed dams showed comparable body weights and weight gain to control pups. The viability index on postpartum d 4 was 97%. Under conditions of this study, the no-observed-adverse-effect level (NOAEL) for exposure to light catalytic reformed naphtha distillate for parental effects was 2500 ppm and the NOAEL for reproductive and developmental toxicity was 7500 ppm.     

竹黄软膏的制备与治疗尿布皮炎的临床应用

目的：研制竹黄软膏，并观察其临床疗效。方法：黄柏和淡竹叶粉碎并回流提取后，加入自制乳膏基质搅匀，制备竹黄软膏，建立其质量控制方法，并进行临床疗效观察。结果：制备工艺简单可行，质量控制方法简便、可靠，临床应用总有效率97.7%。结论：该制剂疗效肯定，使用方便。     

Toxicity evaluation of petroleum blending streams: reproductive and developmental effects of light catalytic reformed naphtha distillate in rats

A distillate of light catalytic reformed naphtha (CAS number 64741-63-5, LCRN-D) administered by inhalation was tested for reproductive and developmental toxicity in Sprague-Dawley rats, following a modified OECD Guideline 421, Reproductive/Developmental Toxicity Screening protocol. LCRN-D was administered as a vapor, 6 h/d, 7 d/wk at target concentrations of 0, 750, 2500 or 7500 ppm to female rats for approximately 6 wk from 2 wk prior to mating, during mating through gestational d 19, and to males beginning 2 wk prior to mating for approximately 7 consecutive weeks. Dams and litters were sacrificed on postnatal d 4 and males were sacrificed within the week after the last litter was necropsied. Parental systemic effects observed at the 7500 ppm exposure level included slightly lower body weights for males throughout the study. Increased kidney to body weight and increased liver to body weight ratio in male rats exposed to 7500 ppm LCRN-D may be related to slightly lower final mean body weights. Body and organ weight data for female rats in all exposure groups were comparable to controls. No test-material-related microscopic changes were observed in the reproductive organs or nasal turbinate tissue of either sex. Reproductive performance was unaffected by exposure to LCRN-D. The mating and fertility indices were 100% in all groups. There were no significant exposure-related differences in implantation sites or live pups per litter, and no gross abnormalities were observed in pups from treated dams. Pups born from LCRN-D-exposed dams showed comparable body weights and weight gain to control pups. The viability index on postpartum d 4 was > or =97%. Under conditions of this study, the no-observed-adverse-effect level (NOAEL) for exposure to light catalytic reformed naphtha distillate for parental effects was 2500 ppm and the NOAEL for reproductive and developmental toxicity was 7500 ppm.     

阿昔洛韦眼膏的制备及质量控制

目的:制备阿昔洛韦眼膏并建立质量控制方法。方法:以凡士林,羊毛脂,液体石蜡为基质制备阿昔洛韦眼膏,用反相高效液相色谱法测定阿昔洛韦的含量。结果:测定阿昔洛韦的平均回收率为98.47％,RSD为1.20％(n=5);制剂无刺激,稳定性好。结论:本制剂工艺简单,质量稳定、可控,能适应临床需要,以反相高效液相色谱法测定阿昔洛韦的含量准确、快速。     

Effect of isepamicin (HAPA-B) on reproduction. I. Fertility study in rats (intramuscular administration)

Effect of isepamicin (HAPA-B), a new aminoglycoside antibiotic, on fertility and reproductive performance in Wistar rats was studied. The drug was injected intramuscularly to rats at a daily dose of 25, 100 or 200 mg/kg. Male rats were treated for 63 days before mating and during the mating period, and female rats were treated for 2 weeks and through the mating period to the 7th day after mating. In male rats, an inhibition of body weight gain and a development of a pale color and hypertrophy of the kidney were observed in 100 and 200 mg/kg dose-groups. In female rats, inhibition of body weight gain was observed in 100 and 200 mg/kg dose-groups and a pale color of the kidney was seen in the 200 mg/kg group. No significant differences were observed in mating and fertility performance of male and female rats between the control and treated groups. Numbers of corpora lutea, implantation sites and living fetuses in the 200 mg/kg group decreased significantly. No external, visceral and skeletal anomalies due to the treatment of HAPA-B were observed in the live fetuses. No effect dose levels of HAPA-B found in this study were 25 mg/kg on parent rats and 100 mg/kg on reproduction ability and their fetuses.