子宫内膜异位症组织中Survivin蛋白表达与凋亡的关系

目的研究子宫内膜异位症(EM)患者在位、异位内膜组织以及正常子宫内膜中的Survivin蛋白表达和细胞凋亡状况,探讨它们在子宫内膜异位症发生中的作用及临床意义。方法39例子宫内膜异位患者的39份异位子宫内膜及21份在位内膜为研究组;26例非子宫内膜异位症患者的子宫内膜为对照组。Survivin蛋白表达用免疫组化法检测;细胞凋亡用TUNEL法检测。结果EM组在位及异位内膜增生期Survivin蛋白表达水平分别明显高于同期正常子宫内膜水平(P<0.05)。EM组在位及异位内膜分泌期Survivin蛋白表达水平分别明显高于同期正常子宫内膜水平(P<0.05)。EM组在位及异位内膜增生期凋亡指数(AI)分别明显低于同期正常子宫内膜(P<0.05)。EM组在位及异位内膜分泌期AI分别明显低于同期正常子宫内膜(P<0.05)。EM组在位及异位内膜Survivin蛋白表达水平均与AI负相关(P<0.05)。结论子宫内膜异位症患者子宫内膜组织细胞凋亡水平下降。凋亡水平的下降导致经期脱落的子宫内膜细胞异位生存和种植的能力增强。子宫内膜异位症患者Survivin蛋白表达上调是导致其凋亡水平下降的机制之一。     

Survivin和MMP-9在子宫内膜异位症中的表达及相关性分析

目的通过检测Survivin（凋亡抑制基因）及MMP-9（基质金属酶蛋白9）在子宫内膜异位症（endom-otriosis,EMS）患者在位内膜、异位内膜组织中的表达,探讨其在子宫内膜异位症发生发展中的作用及两者的相关性。方法采用免疫组化二步法检测40例患者异位子宫内膜组织、在位内膜组织及40例正常子宫内膜（单纯子宫肌瘤）组织中Survivin及MMP-9的表达情况,Survivin及MMP-9二者相关性检验采用Spearman等级相关。结果 Survivin在子宫内膜异位症患者异位内膜、在位内膜及正常子宫内膜组织中阳性表达率分别为77.5%、60.0%和7.5%;MMP-9在三组子宫内膜阳性表达率分别为72.5%、62.5%和20.0%;两种蛋白在三组中的表达差异具有统计学意义（P〈0.05）。Survivin与MMP-9在子宫内膜异位症中表达呈正相关（P〈0.05）。结论 Survivin蛋白和MMP-9在子宫内膜异位症患者的在位及异位内膜均高表达,二者在子宫内膜异位症的发生发展中有协同作用。     

Survivin在子宫内膜异位症的表达及意义

目的:探讨凋亡抑制基因survivin在子宫内膜异位症中的表达及意义.方法:应用免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法(SP法),检测40例子宫内膜异位症异位子宫内膜组织中survivin蛋白的表达,并与正常在位子宫内膜组织25例进行对照.结果:子宫内膜异位症异位内膜组织中survivin蛋白阳性表达率为67.5%,明显高于正常在位内膜组织的阳性表达率4.2%(P＜0.01),并且异位内膜组织中survivin蛋白表达与月经周期无相关性(P＞0.05).结论:survivin在子宫内膜异位症异位内膜中高表达.并且可能通过抑制异位内膜细胞凋亡,对子宫内膜异位症的发生、发展起作用.     

妇科 子宫内膜异位症

061907MMP-2、TIMP-2在子宫内膜异位症组织中的表达及其意义/刘维琴…∥现代妇产科进展·—2006,15(8)·—589~591为研究基质金属蛋白酶-2(MMP-2)及其抑制因子-2(TIM-2)mRNA在子宫内膜异位症异位和在位内膜组织中的表达及临床意义。分别选取子宫内膜异位症异位内膜组织50位、在位内膜组织26例和对照组正常子宫内膜组织22例,用RT-PCR法检测MMP-2、TIMP-2mRNA的表达。结果:(1)MMP-2mRNA在异位症异位内膜组织中相对表达量明显高于正常子宫内膜组织和在位内膜(P<0·01,P<0·01);TIMP-2mRNA在子宫内膜异位症异位内膜和在位内…     

Survivin在子宫内膜异位症的表达及意义

目的:探讨凋亡抑制基因survivin在子宫内膜异位症中的表达及意义.方法:应用免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法(SP法),检测40例子宫内膜异位症异位子宫内膜组织中survivin蛋白的表达,并与正常在位子宫内膜组织25例进行对照.结果:子宫内膜异位症异位内膜组织中survivin蛋白阳性表达率为67.5%,明显高于正常在位内膜组织的阳性表达率4.2%(P＜0.01),并且异位内膜组织中survivin蛋白表达与月经周期无相关性(P＞0.05).结论:survivin在子宫内膜异位症异位内膜中高表达;并且可能通过抑制异位内膜细胞凋亡,对子宫内膜异位症的发生、发展起作用.     

凋亡抑制蛋白Survivin和Bcl-2在子宫内膜异位症中的表达及相关性研究

目的了解凋亡抑制蛋白Survivin和Bcl-2在子宫内膜异位症（EMs）中的表达及相关性。方法应用SP免疫组化方法检测在位内膜、异位内膜、及正常子宫内膜分别在增生期、分泌期凋亡抑制蛋白Survivin和Bcl-2的阳性率。结果凋亡抑制蛋白Survivin、Bcl-2分别在正常子宫内膜中无论是增生期还是分泌期均未见阳性表达,但凋亡抑制蛋白Survivin、Bcl-2在在位子宫内膜、异位子宫内膜中无论是增生期还是分泌期均存在阳性表达,且凋亡抑制蛋白Survivin、Bcl-2在异位子宫内膜中无论是增生期还是分泌期的阳性率均分别高于在位内膜的阳性率．差异具有统计学意义（P〈0．05））。但Survivin、Bcl-2分别在异位内膜的增生期与分泌期阳性率比较,差异无统计学意义（P〉0．05）。子宫内膜异位症组织中survivin蛋白表达与Bcl-2表达呈正相关（r=0．785,P=0．000）。结论Survivin、Bcl-2二者在异位内膜中共同协作抑制了异位内膜的凋亡,从而参与了子宫内膜异位症的发病过程。     

Survivin、Bcl-2、Bax在子宫腺肌病组织中的表达及意义

目的 检测Survivin、Bcl-2、Bax在子宫腺肌病(adenomyosis)组织中的表达,探讨这三者为核心的细胞增殖及凋亡异常在子宫腺肌病发病机制中的作用及相关意义.方法 采用免疫组织化学S-P法检测45例子宫腺肌病异位及在位内膜组织中及对照组10例正常在位子宫内膜组织中Survivin、Bcl-2、Bax的表达.结果 子宫腺肌病异位内模组织中survivin蛋白阳性表达率为80%.显著高于正常在位内膜组织的表达率20%(P<0.05).Survivin蛋白表达与Bcl-2蛋白呈正相关(p<0.05).而与Bax蛋白呈负相关(P<0.05).结论 Survivin可通过抑制异位内膜细胞凋亡,对子宫腺肌病的发生、发展起着重要作用,可能成为子宫腺肌病基因治疗的新靶点.Survivin基因可能与凋亡相关基因Bcl-2、bax在子宫腺肌病的发生、发展中分别起协同和拮抗作用.     

Survivin在子宫腺肌病的表达及其与Bcl-2、Bax相关性的研究

目的 探讨凋亡抑制基因Survivin在子宫腺肌病的表达及其与Bcl-2、Bax的相关性.方法 应用免疫组织化学S-P法,检测46例子宫腺肌病异位及在位内膜组织中Survivin、Bcl-2、Bax蛋白的表达,并与正常在位子宫内膜组织30例进行对照.结果 子宫腺肌病异位内膜组织中Survivin蛋白阳性表达率为69.57%,明显高于正常在位内膜组织的表达率6.67%(P＜0.05),并且异位内膜组织中Survivin蛋白表达与月经周期无相关性(P＜0.05);Survivin蛋白表达与Bel-2蛋白呈正相关(P＜0.01),而与Bax蛋白呈负相关(P＜0.01).结论 Survivin可通过抑制异位内膜细胞凋亡,对子宫腺肌病的发生、发展起作用;Survivin与Bcl-2、Bax在子宫腺肌病的发生、发展中分别起协同和拮抗作用.     

Survivin在子宫内膜异位症的表达及其与bcl-2、bax相关性的研究

目的 探讨凋亡抑制基因survivin在子宫内膜异位症的表达,及其与bcl-2、bax的相关性. 方法应用免疫组织化学S-P法,检测40例子宫内膜异位症异位内膜组织中survivin、bcl-2、bax蛋白的表达,并与正常在位子宫内膜组织25例进行对照.结果 子宫内膜异位症异位内膜组织中survivin蛋白阳性表达率为67.5%,明显高于正常在位内膜组织的表达率4.2%(P＜0.05),并且异位内膜组织中survivin蛋白表达与月经周期无相关性(P＜0.05);survivin蛋白表达与bcl-2蛋白呈正相关(P＜0.05),而与bax蛋白呈负相关(P＜0.05). 结论 survivin可通过抑制异位内膜细胞凋亡,对子宫内膜异位症的发生、发展起作用;survivin与bcl-2、bax在子宫内膜异位症的发生、发展中分别起协同和拮抗作用.     

MMP-9及TIMP-1在子宫内膜异位症中的表达及意义

目的 :观察MMP 9和TIMP 1在子宫内膜异位症中的表达 ,明确二者之间的相关性探讨他们在子宫内膜异位症发病机制中的作用 .方法 :利用免疫组化SABC法检测MMP 9和TIMP 1在 84例正常子宫内膜、4 8例内异症的在位内膜、79例内异症的异位内膜组织中的表达 ,检测结果利用SPLM软件进行统计学分析 .结果 :①MMP 9在内异症在位内膜中的阳性表达率为 62 .5 0 % ,内异症异位内膜中阳性表达率为 72 .15% ,与正常内膜组织的表达 (阳性率为 4 0 .4 8% )相比有显著性差异 (P <0 .0 5 ) ;②TIMP 1在异位症在位内膜中的阳性表达率为 70 .83 % ,在异位症异位内膜中阳性表达率为 74 .68% ,与正常内膜组织的表达 (阳性率为 76.19% )相比未见显著性差异 (P >0 .0 5 ) ;③在正常子宫内膜组织中MMP 9与TIMP 1之间具有负向相关关系 ,在异位症的在位内膜组织中以及异位症的异位内膜组织中二者间不具有相关性 .结论 :MMP 9的高表达以及MMP 9/TIMP 1间的比例失调 ,在内异症的病程发展进程中起着重要作用 ,是内异症来源的在位内膜及异位内膜细胞具有更强的侵袭和异位种植能力的原因之一     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.