骨碎补中的化学成分

目的研究骨碎补（Drynaria fortunei）大孔吸附树脂柱色谱以体积分数50％乙醇洗脱部分的化学成分。方法利用硅胶、Sephadex LH-20、ODS柱色谱及制备型HPLC等方法进行分离纯化，根据化合物的理化性质及波谱数据鉴定结构。结果与结论从骨碎补大孔吸附树脂柱色谱50％乙醇洗脱部分分离鉴定了7个已知化学成分。分别为：山柰酚7-O-α-呋喃阿拉伯糖（1）、紫云英苷（2）、阿福豆苷（3）、北美圣草索（4）、3-乙酰胺基-4-羟基苯甲酸（5）、5-乙氧基-2-羟基苯甲酸乙酯（6）、β-谷甾醇（7）。化合物1～6为首次从该属植物中分离得到。     

骨碎补化学成分和药理作用研究进展

骨碎补为常用中药,化学成分类型多样,主要涉及黄酮、三萜、酚酸及其苷类等,具有良好的促进骨折愈合、抗骨质疏松、抗炎等活性,研发前景广阔。本文对骨碎补的化学成分和药理作用进行综述,为其进一步的研究与开发奠定基础。     

中药连翘的化学成分研究

目的:对中药连翘的化学成分进行研究。方法:利用大孔吸附树脂、硅胶柱色谱、Sephadex LH-20柱色谱、ODS柱色谱和制备液相等色谱手段进行分离纯化,根据化合物的理化性质和波谱学等方法鉴定其结构。结果:从木犀科连翘属植物连翘的95%乙醇提取物中分离得到6个化合物,分别鉴定为对羟基苯甲酸(1),对乙醇基苯甲醛(2),对羟基苯乙酸(3),对香豆酸(4),对羟基苯基乙酸乙酯(5),1-(4-羟基苯基)-2,3-二羟基丙酮(6)。结论:化合物2、5、6为首次从连翘属(Forsythia)中分离得到。     

益母草化学成分的分离与鉴定

目的分离、鉴定益母草(Leonurus heterophyllusSweet.)提取物中的化学成分。方法采用硅胶柱色谱法、SephadexLH-20柱色谱法等分离,并通过1H-NMR、13C-NMR等谱学技术确定其结构。结果分离得到7个化合物,分别鉴定为薰衣草叶苷(lavandulifolioside,1)、芦丁(rutin,2)、苯甲酸(benzoic acid,3)、邻羟基苯甲酸(salicylic acid,4)、丁香酸(syringic acid,5)、腺苷(adenosine,6)、豆甾醇(stigmasterol,7)。结论其中化合物3、4、6为首次从益母草属中分离得到,1、2、5为首次从本种植物中分离得到。     

山楂核化学成分的分离与鉴定

目的对山楂(Crataegus pinnatifida Bge.)核中的化学成分进行研究。方法运用硅胶﹑Seph-adex LH-20、ODS柱色谱,制备HPLC,重结晶等分离手段进行分离纯化。根据理化性质及波谱数据鉴定其结构。结果从山楂核体积分数为70%乙醇提取物中分离得到4个化合物,分别鉴定为threo-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(1)、erythro-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-pro-panediol(2)、3-(4-hydroxy-3-methoxyphenyl)-3-methoxypropane-1,2-diol(3)、2-[4-(3-hydroxyprop-yl)-2-methoxyphenoxy]propane-1,3-diol(4)。结论化合物1～4为首次从山楂属植物中分离得到。     

芫花花蕾化学成分的分离与鉴定

目的对中药生品芫花(Daphne genkwaSieb.et Zucc.)花蕾中的化学成分进行研究。方法运用硅胶、Sephadex LH-20柱色谱、ODS柱色谱、制备HPLC、重结晶等分离手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果从芫花花蕾体积分数95%乙醇提取物中分离得到10个化合物。分别鉴定为8-甲氧基山奈酚(8-methoxykaempferol,1)、柚皮素(naringenin,2)、芫花素(genkwanin,3)、5,4′-二羟基-7,3′-二甲氧基黄酮(velutin,4)、芫花素-5-O-β-D-葡萄糖苷(genkwanin-5-O-β-D-glucoside,5)、芫花素-5-O-β-D-茜黄樱草糖苷(genkwanin-O-β-D-pri-meveroside,6)、(-)-松脂素((-)-pinoresinol,7)、(-)-落叶松脂素((-)-lariciresinol,8)、(-)-双氢芝麻脂素((-)-dihydrosesamin,9)、瑞香烷型二萜酯-7(daphnane-type diterpene ester-7,10)。结论化合物1为首次从瑞香属植物中分离得到,化合物2,9,10为首次从芫花中分离得到。     

藜的化学成分研究

目的：对藜Chenopodium olbumL.的抗过敏活性成分进行研究。方法：利用色谱的方法分离成分。利用光谱和化学方法鉴定其化学结构。结果：从藜的种子中分离得到3个化合物。经光谱和化学分析，鉴定为28-氧-β-D-吡喃葡萄糖基-齐墩果酸-3-氧-β-D-吡喃葡萄糖醛酸甙（1），20-羟基蜕皮酮（2）和芦丁（3）。结论：化合物1，2和3均为首次从该植物分离得到。     

萱草根化学成分的分离与结构鉴定

目的 研究萱草根的化学成分。方法 通过理化性质和光谱数据的分析鉴定了12个化合物的结构。结果与结论 自萱草根[Hemerocallis fulva(L.)L.]中分离得到12个化合物。分别鉴定为7-hydroxynaphthalide(1)，3α-乙酰基-11-氧代-12-乌苏烯-24-羧酸(2)，3-氧代羊毛甾-8，24-二烯-21-羧酸(3)，3β-羟基羊毛甾-8，24-二烯-21-羧酸(4)，3α-羟基羊毛甾-8，24-二烯-21-羧酸(5)，β-谷甾醇(6)，25(R)-螺甾烷-4-烯-3，12-二酮(7)，2’，4，6’-三羟基-4’-甲氧基-3’-甲基二氢查耳酮(8)，α-乳香酸(9)，β-乳香酸(10)，11α-羟基-3-已酰基-β-乳香酸(11)及脂肪族化合物(12)。除化合物1，6，8，12外其余均为首次从该属植物中分得。化合物2，9，10，11的^13C-NMR数据为首次报道。     

毛金竹叶提取物化学成分的分离与鉴定

目的分离、鉴定毛金竹(Phyllostachys nigra(Lodd.exLindl.)Munro var.henonis(Mitf.)Stepfex Rendle)竹叶提取物的化学成分。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱、开放ODS柱色谱、制备薄层色谱等方法进行分离纯化,并通过理化性质和光谱数据鉴定其化学结构。结果从其乙酸乙酯萃取层和正丁醇萃取层分离得到12个化合物,分别鉴定为β-谷甾醇(β-sitosterol,1)、苜蓿素(tricin,2)、胡萝卜苷(daucosterol,3)、丁二酸(butanedioic acid,4)、胸腺嘧啶(5-methylu-racil,5)、苜蓿素-7-O-β-D-葡萄糖苷(tricin-7-O-β-D-glucopyranoside,6)、牡荆苷(vitexin,7)、苜蓿素-7-O-新橙皮糖苷(tricin-7-O-neohesperidoside,8)、荭草苷(orientin,9)、异荭草苷(isoorientin,10)、尿嘧啶(uracil,11)、胸腺嘧啶脱氧核苷(thymidine,12)。结论化合物4~6、8、11、12为首次从该属植物中分离得到。     

南海海绵Spheciospongia vagabunda化学成分的研究(Ⅰ)

从中国南海海绵Spheciospongia vagabunda中分离出4个化合物，应用^1H—NMR，^13C—NMR，IR，MS等光谱分析技术对它们的结构进行了鉴定，确定它们为：胆甾醇(Ⅰ)、24(s)-豆甾-5-烯-3β-醇(Ⅱ)、对羟基苯甲醛(Ⅲ)、邻苯二甲酸二丁酯(Ⅳ)。本文首次报道中国南海该种属海绵化学成分的分离提纯和结构鉴定。     

骨碎补中的苯丙素类成分及其对UMR 106细胞增殖作用的影响

目的研究骨碎补(Drynaria fortunei)中的苯丙素类成分,并讨论它们对大鼠类成骨细胞UMR 106增殖的影响。方法采用硅胶、Sephadex LH-20、ODS柱色谱分离骨碎补根茎的活性成分,应用理化方法及1D-NMR、2D-NMR方法确定化学结构,并检测对UMR 106细胞增殖的影响。结果从骨碎补体积分数为60%乙醇提取物中的大孔吸附树脂柱色谱的体积分数为30%乙醇洗脱部分,分离得到7个苯丙素类化合物和2个苯甲酸类化合物,分别鉴定为:(E)-4-O-β-D-吡喃葡萄糖基反式咖啡酸(1)、(E)-p-松针酸β-D-吡喃葡萄糖苷(2)、阿魏酸β-D-吡喃葡萄糖苷(3)、反式咖啡酸(4)、p-香豆酸4-O-β-D-吡喃葡萄糖苷(5)、二氢异阿魏酸(6)、二氢咖啡酸(7)、香草酸4-O-β-D-吡喃葡萄糖苷(8)、原儿茶酸(9)。并检测了化合物1~9对UMR 106细胞增殖的影响。结论化合物1首次从该种植物中分离得到,化合物2~8首次从该属植物中分离得到。化合物1~4显示了对UMR 106细胞的促增殖作用,而其他化合物则没有显示活性。     

Chemical constituents from Paliurusramosissimus

In the present research, in order to study the chemical constituents of Paliurusramosissimus(Lour.) Poir., the isolation of its ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were elucidated on the basis of combination of mass spectrometry and1D,2DNMRspectroscopy. A total of 8 compounds were obtained, and their structures were identified as 3β-hydroxy-27-(3,4-dihydroxybenzoyl)oxylup-20(29)-en-28-oic acid(1),betulinic acid (2), lupeol(3), 27-O-trans-caffeoylcylicodiscic acid (4), ceanothic acid (5), 24-hydroxyceanothic acid (6), dihydrokaempferol (7), eriodictyol (8). Among them, compound 1 was a new compound. Compounds 3–4, 7–8 were isolated from Paliurus ramosissimus (Lour.) Poir. for the first time.     

Chemical constituents from Teucrium viscidum

A new monoterpenoid, 4,​5,​8-​trihydroxy-​6(7)-en-​decenoic acid γ-​lactone (1) and six known compounds, 3,7-dimethyloct-1-ene-3,6,7-triol (2), 2,8-bornanediol (3), 1-(4-hydroxyphenyl)ethane-1,2-diol (4),2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-diol(5), indole-3-carboxylic acid (6), and 3-hydroxy-benzenemethanol (7) were isolated from the whole parts of Teucrium viscidum. Their structures were established by a combination of spectroscopic data analysis, besides comparison with literature data. Compounds 2–7 described above were isolated from this genus for the first time.     

王瓜化学成分研究（英文）

本文对葫芦科植物王瓜化学成分进行了研究,分离得到1个新化合物, 9个已知化合物。通过波谱解析、理化常数及比对文献数据资料进行结构鉴定,分别为3β-[(E)-caffeoyloxy]-D:C-friedooleana-7,9(11)-dien-29-oicacid(1),cucurbitacin B (2), 23,24-dihydrocucurbitacin B (3), 23,24-dihydrocu-curbitacin D (4), cucurbitacin D (5), cucurbalsaminol A (6),3-epi-isocucurbitacin D (7), curbitacin G (8), cucurbitacin J (9) and cucurbitacin I (10),所有化合物均首次从该植物中分离得到。     

Chemical constituents of Euonymus fortunei

A new flavonol glycoside, kaempferol-3-O-β-d-(2-O-E-p-coumaroyl)-glucopyranosyl-7-O-α-l-rhamnopyranoside (1), along with eleven known compounds including five flavonol glycosides (2–6), one phenolic glycoside (7), two megastigmane glycosides (8 and 9), two triterpenoids (10 and 11) and one alditol (12), was isolated from the aerial parts of Euonymus fortunei. Their structures were determined on the basis of spectroscopic analysis and chemical evidence. Compounds 2–4, 7, 8, and 10–12 were evaluated their antimicrobial activities against Ureaplasma urealyticumin vitro, but all tested compounds have no useful activities against Ureaplasma urealyticum.     

Chemical constituents from Croton crassifolius Geisel

Croton crassifolius Geisel belongs to the genus croton of the family euphorbiaceae. By means of solvent extraction and repeated chromatorgraphy on silica gel, Sephadex LH-20 and HPLC, the ethanol extract of the roots of Croton crassifolius Geisel was chemically investigated, which led to the isolation of nine diterpenoids. By using spectroscopic analysis, their structures were identified as follows: Crassifolin P (1), Crassifolin J (2), 1,5,6-trimethyl-5-(2-(5-oxo-2,5-dihydrofuran-3-yl)ethyl)-1,2,3,4,5,6,7,8-octahydronaphthalene-1-carboxylate (3), Penduliflaworosin (4), isoteucvin (5), Crassifolin G (6), teucvin (7), Chettaphanin I (8), mallotucin D (9). Among of them compound 1 was a new compound.     

Chemical constituents from the stem of Embelia laeta

In the present study, we aimed to investigate the chemical constituents from Embelia laeta. The constituents were separated by various column chromatography, including silica gel, ODS gel, MCI gel, Sephadex LH-20, and semipreparative HPLC. Their structures were elucidated by spectroscopic means. A total of 13 compounds were isolated from E. laeta and identified as (6R,9R)-1-Oxo-α-ionol-9-O-β-D-goucopyranoside (1), (+)-Pinoresinol (2), Leptolepisol D (3), Kaempferol (4), Kaempferol-3-α-L-Ramnopyranoside (5), Kaempferol-3-β-D-(6-O-trans-p-coumaroyl) glucopyranoside (6), Bis(2-ethylhexyl) phthalate (7), 2-Benzenedicarboxylic diisobutyl ester (8), Caffeic acid methyl ester (9), Paldehyde (10), Coniferaldehyde (11), Caffeic acid (12) and 5-Hydroxymethyl furfural (13), respectively. All the compounds were isolated from E. laeta for the first time.     

Chemical constituents isolated from the endophytic fungus Penicillium commune

Phytochemical investigation of the endophytic fungus Penicillium communeled to the isolation of nine known compounds.Based on the spectroscopic and chemical properties, their structures were identified as 6-acetyl-2α,5-dihydroxy-2-(2-hydroxypropyl)-3α,8-dimethylchroman (1), communol B (2), communol E (3), communol G (4), 2-hydroxy-4-phenylquinoline (5), viridicatol (6), 3-O-methylviridicatin (7), trans-ferulic acid (8), 1,2-benzenediol,4-(2-methoxyethenyl) (9). Compounds 1 and 5-9 were isolated from this fungus for the first time.